Rh7DG374000

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000192
Physical Location & Seq
Forward (+)
50749680 .. 50752340
2661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG374000.1

Sequence Viewer

Length: 1077 bp
ATGGAAATGGAGATGCTTATCCTTTCTGTGCTAGTTCTCTTTGTTTCTCTCTACTATGTTTTTCGTATGTTGAATAGAAATGCTAGCAAAAATGAAGTTCATCATCATCATCAGCTTCAGCTCCCACCAGGAAGCTCAGGTTGGCCCATCATCGGTGAAGGCCTTGCTTTTTTGATGACAGCCAAAAGGGGTGTCCCGGAGAAGTTCATAGGAGAAAGGAGGATCAAGTACTCATCACCAGATTCCAAAAGCAAAAGCAGCAGCTGCAAGGTGTTCACCACATCGTTGCTGCGTGAATCCATGGCGGTGCTATGTTCGGCCGCTGGAAACAAGTTCCTGTTTTCGAACGAGAACAAACTCGTCAAGTCATGGTGGCCTGCTAACATCGACACCATCTTTGCCAGCTCCAGCAAAACAAACACCGCCGAGGAGTCCATTCGTCACCGCAAAGTTCTATCTCCGTTTCTCAGCCCCGATTCTCTCCGAAAGTACTTAGGGATCATGGATGCTGTCACCAAACGTCATCTGGACCTGTTCTGGTCGGATAAAAAACAAGTCAAAGTTCTAGCCCTAACAAAGATGCACACCTTCACATTGGTCTGTAAAATCTTCTTAAACGTGGAGGATCCAGCAGTGATTGCCAAACTAGAGAAGTCAATTCAGCATATTGCAGCAGGCTTCATTTCATTGCCTATAAATCTGCCTGGCACAGCATTCAACCGAGCCATCCAAGCATCCAAGCAGGTCAGGAAGGACCTTGAAAAAATGGTTAAACAAAGGAGAATAGATCTAGATACAGCTGTGGCAGCAGATGAAAATAATAATAATCAAGATTTAATGTCTAAGTTGCTGCTGGAGAAATATAGCGACGGCCAACAAATGAACGAAACCGACATCGCCAACAAGTTAAATGGTCTACTCATTGCTGCTTATGACAATATATTCATCACCTTGTGTTCTATCATCATGTATCTATCGGAGCTTCCTACAGTTTATGATGCCGTCCTTAAAGGTACCTACAGATTAAAAGCATTGTTTTTCTTTTTTGGACAATCTGGCTACATATTTCAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

358

Amino Acids

40.45

Weight (kDa)

9.4

Isoelectric Point (pI)

44.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 90 - 335 1.7e-12 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000192)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36110 AT5G36110 AT5G36130 AT5G36140
fragaria_vesca FvH4_2g39160 FvH4_3g15030 FvH4_3g38021 FvH4_3g38080 FvH4_4g00250 FvH4_4g01400 FvH4_5g18260 FvH4_5g18260 FvH4_5g29490 FvH4_7g03260 FvH4_7g12550 FvH4_7g12560 FvH4_7g14310 FvH4_7g14330
malus_domestica MD04G1002500.v1.1 MD11G1006100.v1.1 MD11G1157200.v1.1 MD11G1157300.v1.1 MD13G1116400.v1.1 MD13G1219600.v1.1 MD13G1284800.v1.1 MD16G1278300.v1.1 MD17G1219500.v1.1
prunus_persica Prupe.1G002500_v2.0.a1 Prupe.6G120400_v2.0.a1
pyrus_communis pycom04g00270 pycom10g20130 pycom11g12750 pycom16g24900 pycom17g22420 pycom420g00590
rosa_chinensis RchiOBHm_Chr1g0325391 RchiOBHm_Chr1g0340921 RchiOBHm_Chr1g0355301 RchiOBHm_Chr1g0355311 RchiOBHm_Chr1g0355331 RchiOBHm_Chr1g0355341 RchiOBHm_Chr2g0153901 RchiOBHm_Chr4g0384961 RchiOBHm_Chr4g0384991 RchiOBHm_Chr4g0393781 RchiOBHm_Chr4g0433801 RchiOBHm_Chr5g0024681 RchiOBHm_Chr5g0024711 RchiOBHm_Chr5g0024721 RchiOBHm_Chr5g0024791 RchiOBHm_Chr7g0177321 RchiOBHm_Chr7g0177351 RchiOBHm_Chr7g0177361
rosa_laevigata RLG00000001838 RLG00000005543 RLG00000010261 RLG00000028154 RLG00000028157 RLG00000028158 RLG00000028162 RLG00000030142 RLG00000032812 RLG00000032813 RLG00000032814
rosa_multiflora Rmu_co8235405.1_g000001 Rmu_co8395679.1_g000001 Rmu_co8439995.1_g000001 Rmu_sc0000999.1_g000011 Rmu_sc0001171.1_g000026 Rmu_sc0002766.1_g000009 Rmu_sc0003822.1_g000014 Rmu_sc0004135.1_g000001 Rmu_sc0004135.1_g000004 Rmu_sc0004135.1_g000006 Rmu_sc0004135.1_g000012 Rmu_sc0004135.1_g000017 Rmu_sc0004135.1_g000020 Rmu_sc0004637.1_g000001 Rmu_sc0005310.1_g000005 Rmu_sc0006151.1_g000002 Rmu_sc0007192.1_g000013 Rmu_sc0008633.1_g000002 Rmu_sc0020285.1_g000001
rosa_roxburghii Rroxscaffold_1G00054590 Rroxscaffold_2G00098180 Rroxscaffold_3G00234050 Rroxscaffold_3G00236820 Rroxscaffold_3G00275940 Rroxscaffold_4G00300500 Rroxscaffold_4G00300510 Rroxscaffold_5G00332950 Rroxscaffold_7G00188110
rosa_rugosa Rorug01G0051800 Rorug01G0051900 Rorug01G0052200 Rorug01G0052300 Rorug01G0052300 Rorug01G0246500 Rorug01G0246500 Rorug01G0246600 Rorug02G0411000 Rorug03G0298400 Rorug03G0298400 Rorug06G0404900 Rorug06G0405200 Rorug07G0209500 Rorug07G0227800
rosa_samantha Rh1AG066900 Rh1AG258800 Rh1AG259000 Rh1BG055200 Rh1BG055300 Rh1BG228400 Rh1BG228500 Rh1BG228600 Rh1BG228700 Rh1CG068300 Rh1CG242400 Rh1CG242600 Rh1CG242800 Rh1CG242900 Rh1CG243000 Rh1DG073000 Rh1DG256200 Rh1DG256300 Rh1DG256400 Rh1DG256500 Rh4AG002300 Rh4AG326300 Rh4BG001800 Rh4CG002200 Rh4DG001800 Rh5BG173900 Rh5CG190900 Rh5DG175500 Rh7AG004800 Rh7AG004900 Rh7AG349600 Rh7AG375800 Rh7BG004900 Rh7BG362800 Rh7CG004700 Rh7CG366800 Rh7CG394300 Rh7DG004600 Rh7DG004700 Rh7DG374000
rosa_wichuraiana Rw0G008010 Rw0G008840 Rw1G022840 Rw1G022850 Rw4G000710 Rw4G028240 Rw4G028290 Rw5G015960 Rw7G000400 Rw7G029670 Rw7G031540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 733
Acc65I GGTACC 1 cut(s) 1013
AccB1I GGYRCC 1 cut(s) 1013
AccI GTMKAC 1 cut(s) 916
AciI CCGC 4 cut(s) 305, 321, 423, 445
AclWI GGATC 4 cut(s) 230, 506, 620, 633
AcoI YGGCCR 2 cut(s) 318, 871
AcuI CTGAAG 1 cut(s) 101
AdeI CACNNNGTG 1 cut(s) 954
AfaI GTAC 3 cut(s) 230, 491, 1015
AfiI CCNNNNNNNGG 1 cut(s) 152
AgsI TTSAA 4 cut(s) 73, 718, 761, 1070
AjnI CCWGG 2 cut(s) 127, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 124, 156
AluBI AGCT 7 cut(s) 115, 121, 135, 264, 405, 800, 982
AluI AGCT 7 cut(s) 115, 121, 135, 264, 405, 800, 982
AlwI GGATC 4 cut(s) 230, 506, 620, 633
AlwNI CAGNNNCTG 1 cut(s) 264
AoxI GGCC 5 cut(s) 143, 160, 318, 374, 871
ApeKI GCWGC 8 cut(s) 258, 261, 264, 289, 671, 806, 850, 926
ArsI GACNNNNNNTTYG 4 cut(s) 177, 209, 380, 412
Asp718I GGTACC 1 cut(s) 1013
AspS9I GGNCC 3 cut(s) 144, 529, 754
AsuC2I CCSGG 1 cut(s) 197
AsuHPI GGTGA 6 cut(s) 167, 228, 268, 434, 505, 940
AsuII TTCGAA 1 cut(s) 344
AsuNHI GCTAGC 1 cut(s) 83
AvaII GGWCC 2 cut(s) 529, 754
BaeI ACNNNNGTAYC 2 cut(s) 786, 819
BamHI GGATCC 1 cut(s) 625
BanI GGYRCC 1 cut(s) 1013
BbvI GCAGC 8 cut(s) 251, 270, 273, 276, 683, 818, 837, 913
BccI CCATC 3 cut(s) 155, 401, 734
BceAI ACGGC 2 cut(s) 886, 986
BciT130I CCWGG 2 cut(s) 129, 705
BcnI CCSGG 1 cut(s) 197
BfaI CTAG 5 cut(s) 32, 84, 566, 647, 791
BfmI CTRYAG 2 cut(s) 987, 1018
BfuAI ACCTGC 1 cut(s) 733
BglII AGATCT 1 cut(s) 787
BisI GCNGC 9 cut(s) 259, 262, 265, 290, 321, 672, 807, 851, 927
BlsI GCNGC 9 cut(s) 260, 263, 266, 291, 322, 673, 808, 852, 928
BmcAI AGTACT 2 cut(s) 230, 491
Bme1390I CCNGG 3 cut(s) 129, 197, 705
Bme18I GGWCC 2 cut(s) 529, 754
BmgT120I GGNCC 3 cut(s) 144, 529, 754
BmiI GGNNCC 2 cut(s) 627, 1015
BmrFI CCNGG 3 cut(s) 129, 197, 705
BmsI GCATC 5 cut(s) 3, 496, 570, 743, 988
BmtI GCTAGC 1 cut(s) 87
BpmI CTGGAG 2 cut(s) 391, 875
Bpu10I CCTNAGC 1 cut(s) 136
Bpu14I TTCGAA 1 cut(s) 344
BpuMI CCSGG 1 cut(s) 197
BsaBI GATNNNNATC 1 cut(s) 17
BsaJI CCNNGG 2 cut(s) 300, 426
Bsc4I CCNNNNNNNGG 1 cut(s) 152
Bse3DI GCAATG 2 cut(s) 686, 921
Bse8I GATNNNNATC 1 cut(s) 17
BseBI CCWGG 2 cut(s) 129, 705
BseDI CCNNGG 2 cut(s) 300, 426
BseGI GGATG 3 cut(s) 511, 726, 734
BseJI GATNNNNATC 1 cut(s) 17
BseLI CCNNNNNNNGG 1 cut(s) 152
BseMI GCAATG 2 cut(s) 686, 921
BseMII CTCAG 2 cut(s) 150, 481
BseRI GAGGAG 1 cut(s) 443
BseX3I CGGCCG 1 cut(s) 318
BseXI GCAGC 8 cut(s) 251, 270, 273, 276, 683, 818, 837, 913
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 5 cut(s) 145, 162, 320, 376, 873
BshNI GGYRCC 1 cut(s) 1013
BsiEI CGRYCG 1 cut(s) 321
BsiSI CCGG 1 cut(s) 197
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 152
BsmFI GGGAC 1 cut(s) 179
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 5 cut(s) 145, 162, 320, 376, 873
Bsp119I TTCGAA 1 cut(s) 344
Bsp143I GATC 4 cut(s) 222, 498, 625, 787
Bsp19I CCATGG 1 cut(s) 300
BspACI CCGC 4 cut(s) 305, 321, 423, 445
BspANI GGCC 5 cut(s) 145, 162, 320, 376, 873
BspCNI CTCAG 2 cut(s) 149, 480
BspLI GGNNCC 2 cut(s) 627, 1015
BspMI ACCTGC 1 cut(s) 733
BspOI GCTAGC 1 cut(s) 87
BspPI GGATC 4 cut(s) 230, 506, 620, 633
BspT104I TTCGAA 1 cut(s) 344
BspT107I GGYRCC 1 cut(s) 1013
BsrDI GCAATG 2 cut(s) 686, 921
BssECI CCNNGG 2 cut(s) 300, 426
BssMI GATC 4 cut(s) 222, 498, 625, 787
BssT1I CCWWGG 1 cut(s) 300
Bst2UI CCWGG 2 cut(s) 129, 705
Bst4CI ACNGT 1 cut(s) 991
BstAPI GCANNNNNTGC 2 cut(s) 264, 638
BstBI TTCGAA 1 cut(s) 344
BstC8I GCNNGC 4 cut(s) 85, 378, 403, 676
BstDEI CTNAG 4 cut(s) 136, 467, 493, 843
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 3 cut(s) 511, 726, 734
BstKTI GATC 4 cut(s) 225, 501, 628, 790
BstMBI GATC 4 cut(s) 222, 498, 625, 787
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 5 cut(s) 258, 264, 638, 731, 806
BstNI CCWGG 2 cut(s) 129, 705
BstSCI CCNGG 3 cut(s) 127, 195, 703
BstSFI CTRYAG 2 cut(s) 987, 1018
BstV1I GCAGC 8 cut(s) 251, 270, 273, 276, 683, 818, 837, 913
BstX2I RGATCY 2 cut(s) 625, 787
BstYI RGATCY 2 cut(s) 625, 787
BstZI CGGCCG 1 cut(s) 318
BsuRI GGCC 5 cut(s) 145, 162, 320, 376, 873
BtgI CCRYGG 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 880
BtsCI GGATG 3 cut(s) 511, 726, 734
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 1 cut(s) 639
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 4 cut(s) 85, 378, 403, 676
CaiI CAGNNNCTG 1 cut(s) 264
Cfr13I GGNCC 3 cut(s) 144, 529, 754
Csp6I GTAC 3 cut(s) 229, 490, 1014
CviAII CATG 4 cut(s) 301, 369, 502, 967
CviQI GTAC 3 cut(s) 229, 490, 1014
DdeI CTNAG 4 cut(s) 136, 467, 493, 843
DpnI GATC 4 cut(s) 224, 500, 627, 789
DpnII GATC 4 cut(s) 222, 498, 625, 787
DraIII CACNNNGTG 1 cut(s) 954
EaeI YGGCCR 2 cut(s) 318, 871
EagI CGGCCG 1 cut(s) 318
EclXI CGGCCG 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 162
Eco47I GGWCC 2 cut(s) 529, 754
Eco52I CGGCCG 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 754
EcoRII CCWGG 2 cut(s) 127, 703
EcoT14I CCWWGG 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 4 cut(s) 304, 372, 505, 970
FaqI GGGAC 1 cut(s) 179
FatI CATG 4 cut(s) 300, 368, 501, 966
FblI GTMKAC 1 cut(s) 916
Fnu4HI GCNGC 9 cut(s) 259, 262, 265, 290, 321, 672, 807, 851, 927
FokI GGATG 3 cut(s) 518, 713, 721
Fsp4HI GCNGC 9 cut(s) 259, 262, 265, 290, 321, 672, 807, 851, 927
FspBI CTAG 5 cut(s) 32, 84, 566, 647, 791
GluI GCNGC 9 cut(s) 259, 262, 265, 290, 321, 672, 807, 851, 927
GsuI CTGGAG 2 cut(s) 391, 875
HaeIII GGCC 5 cut(s) 145, 162, 320, 376, 873
HapII CCGG 1 cut(s) 197
Hin1II CATG 4 cut(s) 304, 372, 505, 970
HinfI GANTC 4 cut(s) 242, 296, 431, 476
HpaII CCGG 1 cut(s) 197
HphI GGTGA 6 cut(s) 167, 228, 268, 434, 505, 940
Hpy166II GTNNAC 2 cut(s) 276, 917
Hpy188I TCNGA 3 cut(s) 485, 544, 979
Hpy188III TCNNGA 4 cut(s) 527, 748, 791, 830
Hpy8I GTNNAC 2 cut(s) 276, 917
Hpy99I CGWCG 1 cut(s) 872
HpyAV CCTTC 3 cut(s) 152, 598, 745
HpyCH4III ACNGT 1 cut(s) 991
HpyCH4IV ACGT 2 cut(s) 520, 618
HpyCH4V TGCA 3 cut(s) 267, 583, 671
HpyF10VI GCNNNNNNNGC 5 cut(s) 258, 264, 638, 731, 806
HpyF3I CTNAG 4 cut(s) 136, 467, 493, 843
HpySE526I ACGT 2 cut(s) 520, 618
Hsp92II CATG 4 cut(s) 304, 372, 505, 970
KpnI GGTACC 1 cut(s) 1017
Kzo9I GATC 4 cut(s) 222, 498, 625, 787
LmnI GCTCC 3 cut(s) 126, 410, 979
Lsp1109I GCAGC 8 cut(s) 251, 270, 273, 276, 683, 818, 837, 913
LweI GCATC 5 cut(s) 3, 496, 570, 743, 988
MaeI CTAG 5 cut(s) 32, 84, 566, 647, 791
MaeII ACGT 2 cut(s) 520, 618
MaeIII GTNAC 2 cut(s) 440, 511
MalI GATC 4 cut(s) 224, 500, 627, 789
MboI GATC 4 cut(s) 222, 498, 625, 787
MboII GAAGA 1 cut(s) 601
MflI RGATCY 2 cut(s) 625, 787
MluCI AATT 1 cut(s) 657
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 3 cut(s) 213, 421, 616
MseI TTAA 6 cut(s) 614, 771, 836, 908, 1008, 1025
MslI CAYNNNNRTG 1 cut(s) 305
MspA1I CMGCKG 3 cut(s) 264, 323, 800
MspI CCGG 1 cut(s) 197
MspR9I CCNGG 3 cut(s) 129, 197, 705
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 2 cut(s) 129, 705
MwoI GCNNNNNNNGC 5 cut(s) 258, 264, 638, 731, 806
NciI CCSGG 1 cut(s) 197
NcoI CCATGG 1 cut(s) 300
NdeII GATC 4 cut(s) 222, 498, 625, 787
NheI GCTAGC 1 cut(s) 83
NlaIII CATG 4 cut(s) 304, 372, 505, 970
NlaIV GGNNCC 2 cut(s) 627, 1015
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 2 cut(s) 440, 511
NspV TTCGAA 1 cut(s) 344
PceI AGGCCT 1 cut(s) 162
PctI GAATGC 1 cut(s) 713
PfeI GAWTC 3 cut(s) 242, 296, 476
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 9 cut(s) 260, 263, 266, 291, 322, 673, 808, 852, 928
PleI GAGTC 1 cut(s) 439
PpsI GAGTC 1 cut(s) 439
PpuMI RGGWCCY 1 cut(s) 754
Psp5II RGGWCCY 1 cut(s) 754
Psp6I CCWGG 2 cut(s) 127, 703
PspGI CCWGG 2 cut(s) 127, 703
PspN4I GGNNCC 2 cut(s) 627, 1015
PspPI GGNCC 3 cut(s) 144, 529, 754
PspPPI RGGWCCY 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 264
PsuI RGATCY 2 cut(s) 625, 787
PvuII CAGCTG 2 cut(s) 264, 800
RsaI GTAC 3 cut(s) 230, 491, 1015
RsaNI GTAC 3 cut(s) 229, 490, 1014
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 6 cut(s) 614, 771, 836, 908, 1008, 1025
SatI GCNGC 9 cut(s) 259, 262, 265, 290, 321, 672, 807, 851, 927
Sau3AI GATC 4 cut(s) 222, 498, 625, 787
Sau96I GGNCC 3 cut(s) 144, 529, 754
ScaI AGTACT 2 cut(s) 230, 491
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 129, 197, 705
SfaNI GCATC 5 cut(s) 3, 496, 570, 743, 988
SfcI CTRYAG 2 cut(s) 987, 1018
SfuI TTCGAA 1 cut(s) 344
SinI GGWCC 2 cut(s) 529, 754
SmiMI CAYNNNNRTG 1 cut(s) 305
Sse9I AATT 1 cut(s) 657
SseBI AGGCCT 1 cut(s) 162
SsiI CCGC 4 cut(s) 305, 321, 423, 445
SspMI CTAG 5 cut(s) 32, 84, 566, 647, 791
StuI AGGCCT 1 cut(s) 162
StyD4I CCNGG 3 cut(s) 127, 195, 703
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 1 cut(s) 991
TaiI ACGT 2 cut(s) 523, 621
TaqI TCGA 2 cut(s) 344, 387
TasI AATT 1 cut(s) 657
TatI WGTACW 2 cut(s) 228, 489
TauI GCSGC 1 cut(s) 323
TfiI GAWTC 3 cut(s) 242, 296, 476
Tru1I TTAA 6 cut(s) 614, 771, 836, 908, 1008, 1025
Tru9I TTAA 6 cut(s) 614, 771, 836, 908, 1008, 1025
TscAI CASTG 1 cut(s) 639
TseFI GTSAC 2 cut(s) 440, 511
TseI GCWGC 8 cut(s) 258, 261, 264, 289, 671, 806, 850, 926
Tsp45I GTSAC 2 cut(s) 440, 511
TspDTI ATGAA 8 cut(s) 89, 108, 196, 670, 675, 828, 896, 934
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 1 cut(s) 639
VpaK11BI GGWCC 2 cut(s) 529, 754
XbaI TCTAGA 1 cut(s) 790
XcmI CCANNNNNNNNNTGG 1 cut(s) 523
XmiI GTMKAC 1 cut(s) 916
XspI CTAG 5 cut(s) 32, 84, 566, 647, 791
ZrmI AGTACT 2 cut(s) 230, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.