FvH4_1g19170

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000345
Physical Location & Seq
Forward (+)
11366421 .. 11375880
9460 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g19170.t1

Sequence Viewer

Length: 600 bp
ATGATGAAGAATTCAGTCTTCGTTTTCTTTCTGTCAGTCTATGTCCTTGTCCAACTTATCAAGCTTTCAAATGCTGAACCATTTGAGTACATGCAGTTTGTGCTCCAGTACCCACTTGGAGTTTGTTATGGTACACAAAAAGGTGATTCCAAATCACCTCTGCCTACCAAGTTTACCGTTCACAGCATCTGGCCGTCAAACTTCTCCGATACACGAGTCGTTTGTAGGCAAGCACTGATAAGTCACCCCTTTAATAATGCGCAGATGACGTCGTCTCTGCAGACTGATTTGACAAACTCATGGCCATCTGTGATAACAAAAAAAAGCGACATGCAGTTTTGGCAACATGAGTATGAGGAGCATGGCGCTTGTTCAGTAGACAAGGGTGTACCACTGTTCACCCAGAAGTCCTACTCTGAGAGAGGAAACCAGCTCTGGAACCAATATGACATCCATGCACTGCTTGATCAAAGCAATATCAAACCGGATTCTTCAAAGCCATGCAAAATGACTGAGATTGTAGCTGCAATCCAAAAGAAAATCGGGAAAAACACACTCCTGTACGAAGGTGCAAGGAAGATAACAAAATTTACATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

200

Amino Acids

22.81

Weight (kDa)

8.95

Isoelectric Point (pI)

28.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 185 1.5e-30 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 272
Acc16I TGCGCA 1 cut(s) 261
AccI GTMKAC 1 cut(s) 378
AcoI YGGCCR 2 cut(s) 191, 302
AcsI RAATTY 2 cut(s) 10, 587
AcyI GRCGYC 1 cut(s) 269
AfaI GTAC 5 cut(s) 89, 110, 133, 390, 563
AgsI TTSAA 2 cut(s) 69, 495
AluBI AGCT 3 cut(s) 64, 433, 524
AluI AGCT 3 cut(s) 64, 433, 524
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 1 cut(s) 279
AlwNI CAGNNNCTG 1 cut(s) 189
AoxI GGCC 2 cut(s) 191, 302
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 2 cut(s) 10, 587
AspLEI GCGC 2 cut(s) 262, 368
AsuHPI GGTGA 4 cut(s) 147, 155, 236, 391
BaeI ACNNNNGTAYC 2 cut(s) 201, 234
BalI TGGCCA 1 cut(s) 304
BauI CACGAG 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 511
BccI CCATC 1 cut(s) 313
BceAI ACGGC 1 cut(s) 178
BclI TGATCA 1 cut(s) 466
BcoDI GTCTC 1 cut(s) 279
BfmI CTRYAG 1 cut(s) 278
BfoI RGCGCY 1 cut(s) 369
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
BmiI GGNNCC 1 cut(s) 440
BmsI GCATC 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 10
BpmI CTGGAG 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 269
BsaWI WCCGGW 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 106
BseGI GGATG 1 cut(s) 450
BseMII CTCAG 2 cut(s) 408, 504
BseNI ACTGG 1 cut(s) 106
BseRI GAGGAG 1 cut(s) 371
BseXI GCAGC 1 cut(s) 511
BshFI GGCC 2 cut(s) 193, 304
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 1 cut(s) 485
BsmAI GTCTC 1 cut(s) 279
BsmBI CGTCTC 1 cut(s) 279
BsnI GGCC 2 cut(s) 193, 304
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 1 cut(s) 466
BspANI GGCC 2 cut(s) 193, 304
BspCNI CTCAG 2 cut(s) 409, 505
BspLI GGNNCC 1 cut(s) 440
BspMAI CTGCAG 1 cut(s) 282
BsrI ACTGG 1 cut(s) 106
BssMI GATC 1 cut(s) 466
BssNI GRCGYC 1 cut(s) 269
BssSI CACGAG 1 cut(s) 213
Bst2BI CACGAG 1 cut(s) 213
Bst4CI ACNGT 2 cut(s) 178, 396
BstACI GRCGYC 1 cut(s) 269
BstAPI GCANNNNNTGC 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 2 cut(s) 417, 513
BstF5I GGATG 1 cut(s) 450
BstH2I RGCGCY 1 cut(s) 369
BstHHI GCGC 2 cut(s) 262, 368
BstKTI GATC 1 cut(s) 469
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 2 cut(s) 100, 340
BstNSI RCATGY 2 cut(s) 94, 334
BstSFI CTRYAG 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 1 cut(s) 10
BsuRI GGCC 2 cut(s) 193, 304
BtsCI GGATG 1 cut(s) 450
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 3 cut(s) 233, 392, 458
Cac8I GCNNGC 1 cut(s) 231
CaiI CAGNNNCTG 1 cut(s) 189
CfoI GCGC 2 cut(s) 262, 368
Csp6I GTAC 5 cut(s) 88, 109, 132, 389, 562
CviAII CATG 7 cut(s) 91, 300, 331, 347, 362, 455, 501
CviJI RGCY 6 cut(s) 64, 193, 304, 433, 499, 524
CviKI_1 RGCY 6 cut(s) 64, 193, 304, 433, 499, 524
CviQI GTAC 5 cut(s) 88, 109, 132, 389, 562
DdeI CTNAG 2 cut(s) 417, 513
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
EaeI YGGCCR 2 cut(s) 191, 302
EcoRI GAATTC 1 cut(s) 10
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 7 cut(s) 94, 303, 334, 350, 365, 458, 504
FatI CATG 7 cut(s) 90, 299, 330, 346, 361, 454, 500
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 1 cut(s) 437
Fsp4HI GCNGC 1 cut(s) 525
FspI TGCGCA 1 cut(s) 261
GlaI GCGC 2 cut(s) 261, 367
GluI GCNGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 89
HaeII RGCGCY 1 cut(s) 369
HaeIII GGCC 2 cut(s) 193, 304
HapII CCGG 1 cut(s) 485
HhaI GCGC 2 cut(s) 262, 368
Hin1I GRCGYC 1 cut(s) 269
Hin1II CATG 7 cut(s) 94, 303, 334, 350, 365, 458, 504
Hin6I GCGC 2 cut(s) 260, 366
HinP1I GCGC 2 cut(s) 260, 366
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 3 cut(s) 146, 216, 488
HpaII CCGG 1 cut(s) 485
HphI GGTGA 4 cut(s) 147, 155, 236, 391
Hpy166II GTNNAC 6 cut(s) 134, 174, 181, 379, 389, 399
Hpy188I TCNGA 2 cut(s) 208, 418
Hpy188III TCNNGA 2 cut(s) 436, 544
Hpy8I GTNNAC 6 cut(s) 134, 174, 181, 379, 389, 399
Hpy99I CGWCG 1 cut(s) 274
HpyAV CCTTC 1 cut(s) 560
HpyCH4III ACNGT 2 cut(s) 178, 396
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 7 cut(s) 94, 280, 334, 458, 504, 527, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 340
HpyF3I CTNAG 2 cut(s) 417, 513
HpySE526I ACGT 1 cut(s) 269
Hsp92I GRCGYC 1 cut(s) 269
Hsp92II CATG 7 cut(s) 94, 303, 334, 350, 365, 458, 504
HspAI GCGC 2 cut(s) 260, 366
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 1 cut(s) 466
LmnI GCTCC 2 cut(s) 108, 358
LpnPI CCDG 7 cut(s) 119, 175, 416, 421, 443, 498, 572
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 1 cut(s) 195
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 1 cut(s) 242
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 4 cut(s) 10, 19, 483, 589
MhlI GDGCHC 1 cut(s) 105
MlsI TGGCCA 1 cut(s) 304
MluCI AATT 2 cut(s) 10, 587
MluNI TGGCCA 1 cut(s) 304
MlyI GAGTC 1 cut(s) 225
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 3 cut(s) 168, 349, 416
Mox20I TGGCCA 1 cut(s) 304
MscI TGGCCA 1 cut(s) 304
MseI TTAA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 351
Msp20I TGGCCA 1 cut(s) 304
MspI CCGG 1 cut(s) 485
MwoI GCNNNNNNNGC 2 cut(s) 100, 340
NdeII GATC 1 cut(s) 466
NlaIII CATG 7 cut(s) 94, 303, 334, 350, 365, 458, 504
NlaIV GGNNCC 1 cut(s) 440
NmuCI GTSAC 1 cut(s) 242
NsbI TGCGCA 1 cut(s) 261
NspI RCATGY 2 cut(s) 94, 334
PfeI GAWTC 2 cut(s) 146, 488
PflFI GACNNNGTC 1 cut(s) 271
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 1 cut(s) 224
PpsI GAGTC 1 cut(s) 224
PspN4I GGNNCC 1 cut(s) 440
PstI CTGCAG 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 189
PsyI GACNNNGTC 1 cut(s) 271
RsaI GTAC 5 cut(s) 89, 110, 133, 390, 563
RsaNI GTAC 5 cut(s) 88, 109, 132, 389, 562
RseI CAYNNNNRTG 1 cut(s) 351
SaqAI TTAA 1 cut(s) 252
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 1 cut(s) 466
SchI GAGTC 1 cut(s) 225
SduI GDGCHC 1 cut(s) 105
SetI ASST 7 cut(s) 66, 145, 160, 272, 435, 526, 571
SfaNI GCATC 1 cut(s) 195
SfcI CTRYAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 2 cut(s) 10, 587
TaaI ACNGT 2 cut(s) 178, 396
TaiI ACGT 1 cut(s) 272
TasI AATT 2 cut(s) 10, 587
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 2 cut(s) 146, 488
Tru1I TTAA 1 cut(s) 252
Tru9I TTAA 1 cut(s) 252
TscAI CASTG 3 cut(s) 240, 399, 465
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 1 cut(s) 242
TspDTI ATGAA 1 cut(s) 20
TspRI CASTG 3 cut(s) 240, 399, 465
Tth111I GACNNNGTC 1 cut(s) 271
XapI RAATTY 2 cut(s) 10, 587
XceI RCATGY 2 cut(s) 94, 334
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
XmiI GTMKAC 1 cut(s) 378
ZraI GACGTC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.