Rh3BG073600

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000345
Physical Location & Seq
Reverse (-)
5409183 .. 5433332
24150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG073600.1

Sequence Viewer

Length: 687 bp
ATGGCAATAGTGACATCGTTGTTCTCTCTTTTTATTGTTACTTTTGCTCTCTTCCGGTGTGCCTCTTTCAGCATCGCGAGCTACCAATATTTTCAGTTTGTGCAGCAATGGCCACCAAACTTCTGCTACGGTAGATATCGTCCTTGCAATAGGCAATCATTCACCATTCATGGCCTGTGGCCAAATAATTTTTCACAAGTTAATTACAATCATATTTGTTCAGGGGCCGCATTTAAAAATCAGATGTATTTCAATCAAACACTGGTAAACGACCTGACTCGCTCTTGGCCGGACCTGAGAAATGGGAACCATCAAAGGTTTTGGGAAGAGCAGTGGGATAAACATGGCAAATGCTCGGATCAGACGTATGGGCAAACGCGATACTTCCAGCGCGCCTTCGAGATGTGGAACCAATTCGATATTACTGATATCTTTACTGCCGCTGGCGTCGTATCTCCGCCAGGGACACAACCAAGTAAAGTTAACGCGAGCGACTTAGAAGCACGCATTCAAGCAGTAACTCATATGGCCCCTATCCTTCGGTGCAGAAAAGTTACAGTGCAGAACGTTCAACGGCCAGTTGAGCTAATTTATGAAGTGGGCATTTGTTACGATTATCATGGAACAAATCTGTATCCTTGTACCAGCACACAAGGAAGATGCGGCGTAAATGATATATACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

228

Amino Acids

26.58

Weight (kDa)

8.56

Isoelectric Point (pI)

24.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 214 1.3e-37 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 77, 379, 393, 488
AciI CCGC 4 cut(s) 228, 441, 458, 663
AclI AACGTT 1 cut(s) 567
AclWI GGATC 1 cut(s) 366
AcoI YGGCCR 4 cut(s) 110, 179, 287, 575
AcyI GRCGYC 1 cut(s) 447
AfaI GTAC 1 cut(s) 643
AgsI TTSAA 3 cut(s) 253, 512, 572
AjnI CCWGG 1 cut(s) 460
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 2 cut(s) 81, 586
AluI AGCT 2 cut(s) 81, 586
AlwI GGATC 1 cut(s) 366
AoxI GGCC 7 cut(s) 110, 172, 179, 225, 287, 528, 575
ApeKI GCWGC 1 cut(s) 103
Asp700I GAANNNNTTC 1 cut(s) 413
AspLEI GCGC 2 cut(s) 393, 395
AspS9I GGNCC 3 cut(s) 225, 292, 529
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 292
BalI TGGCCA 2 cut(s) 112, 181
BbvI GCAGC 1 cut(s) 115
BccI CCATC 1 cut(s) 318
BceAI ACGGC 1 cut(s) 590
BciT130I CCWGG 1 cut(s) 462
BciVI GTATCC 1 cut(s) 645
BfuI GTATCC 1 cut(s) 645
BisI GCNGC 4 cut(s) 104, 228, 441, 664
BlsI GCNGC 4 cut(s) 105, 229, 442, 665
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 3 cut(s) 225, 292, 529
BmiI GGNNCC 4 cut(s) 226, 308, 410, 531
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 81, 650
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 461
BsaWI WCCGGW 1 cut(s) 54
Bse1I ACTGG 2 cut(s) 267, 578
Bse3DI GCAATG 1 cut(s) 113
BseBI CCWGG 1 cut(s) 462
BseDI CCNNGG 1 cut(s) 461
BseMI GCAATG 1 cut(s) 113
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 2 cut(s) 267, 578
BsePI GCGCGC 1 cut(s) 391
BseXI GCAGC 1 cut(s) 115
BsgI GTGCAG 3 cut(s) 122, 565, 581
Bsh1236I CGCG 4 cut(s) 77, 379, 393, 488
BshFI GGCC 7 cut(s) 112, 174, 181, 227, 289, 530, 577
BsiSI CCGG 2 cut(s) 55, 290
BslFI GGGAC 1 cut(s) 478
BsmFI GGGAC 1 cut(s) 478
BsmI GAATGC 1 cut(s) 507
BsnI GGCC 7 cut(s) 112, 174, 181, 227, 289, 530, 577
Bsp143I GATC 1 cut(s) 358
Bsp68I TCGCGA 1 cut(s) 77
BspACI CCGC 4 cut(s) 228, 441, 458, 663
BspANI GGCC 7 cut(s) 112, 174, 181, 227, 289, 530, 577
BspCNI CTCAG 1 cut(s) 288
BspFNI CGCG 4 cut(s) 77, 379, 393, 488
BspLI GGNNCC 4 cut(s) 226, 308, 410, 531
BspPI GGATC 1 cut(s) 366
BspQI GCTCTTC 1 cut(s) 321
BsrDI GCAATG 1 cut(s) 113
BsrI ACTGG 2 cut(s) 267, 578
BssECI CCNNGG 1 cut(s) 461
BssHII GCGCGC 1 cut(s) 391
BssMI GATC 1 cut(s) 358
BssNI GRCGYC 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 462
Bst4CI ACNGT 2 cut(s) 131, 559
Bst6I CTCTTC 2 cut(s) 56, 321
BstACI GRCGYC 1 cut(s) 447
BstC8I GCNNGC 5 cut(s) 79, 393, 445, 490, 505
BstDEI CTNAG 2 cut(s) 296, 496
BstFNI CGCG 4 cut(s) 77, 379, 393, 488
BstHHI GCGC 2 cut(s) 393, 395
BstKTI GATC 1 cut(s) 361
BstMBI GATC 1 cut(s) 358
BstMWI GCNNNNNNNGC 3 cut(s) 78, 109, 583
BstNI CCWGG 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 460
BstUI CGCG 4 cut(s) 77, 379, 393, 488
BstV1I GCAGC 1 cut(s) 115
BsuI GTATCC 1 cut(s) 645
BsuRI GGCC 7 cut(s) 112, 174, 181, 227, 289, 530, 577
BtgZI GCGATG 1 cut(s) 58
BtsI GCAGTG 1 cut(s) 338
BtsIMutI CAGTG 3 cut(s) 260, 338, 564
BtuMI TCGCGA 1 cut(s) 77
Cac8I GCNNGC 5 cut(s) 79, 393, 445, 490, 505
CfoI GCGC 2 cut(s) 393, 395
Cfr13I GGNCC 3 cut(s) 225, 292, 529
CseI GACGC 1 cut(s) 436
Csp6I GTAC 1 cut(s) 642
CviAII CATG 3 cut(s) 170, 344, 620
CviJI RGCY 9 cut(s) 81, 112, 174, 181, 227, 289, 530, 577, 586
CviKI_1 RGCY 9 cut(s) 81, 112, 174, 181, 227, 289, 530, 577, 586
CviQI GTAC 1 cut(s) 642
DdeI CTNAG 2 cut(s) 296, 496
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
DraI TTTAAA 1 cut(s) 235
EaeI YGGCCR 4 cut(s) 110, 179, 287, 575
Eam1104I CTCTTC 2 cut(s) 56, 321
EarI CTCTTC 2 cut(s) 56, 321
EciI GGCGGA 1 cut(s) 447
Eco32I GATATC 2 cut(s) 137, 430
Eco47I GGWCC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 460
EcoRV GATATC 2 cut(s) 137, 430
FaeI CATG 3 cut(s) 173, 347, 623
FaqI GGGAC 1 cut(s) 478
FatI CATG 3 cut(s) 169, 343, 619
FauNDI CATATG 1 cut(s) 525
Fnu4HI GCNGC 4 cut(s) 104, 228, 441, 664
Fsp4HI GCNGC 4 cut(s) 104, 228, 441, 664
GlaI GCGC 2 cut(s) 392, 394
GluI GCNGC 4 cut(s) 104, 228, 441, 664
HaeIII GGCC 7 cut(s) 112, 174, 181, 227, 289, 530, 577
HapII CCGG 2 cut(s) 55, 290
HgaI GACGC 1 cut(s) 436
HhaI GCGC 2 cut(s) 393, 395
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 3 cut(s) 173, 347, 623
Hin6I GCGC 2 cut(s) 391, 393
HinP1I GCGC 2 cut(s) 391, 393
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 1 cut(s) 277
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 2 cut(s) 55, 290
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 2 cut(s) 268, 484
Hpy188I TCNGA 3 cut(s) 243, 358, 363
Hpy188III TCNNGA 2 cut(s) 76, 400
Hpy8I GTNNAC 2 cut(s) 268, 484
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 2 cut(s) 406, 548
HpyCH4III ACNGT 2 cut(s) 131, 559
HpyCH4IV ACGT 2 cut(s) 365, 567
HpyCH4V TGCA 4 cut(s) 103, 147, 546, 562
HpyF10VI GCNNNNNNNGC 3 cut(s) 78, 109, 583
HpyF3I CTNAG 2 cut(s) 296, 496
HpySE526I ACGT 2 cut(s) 365, 567
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 3 cut(s) 173, 347, 623
HspAI GCGC 2 cut(s) 391, 393
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 1 cut(s) 358
LguI GCTCTTC 1 cut(s) 321
Lsp1109I GCAGC 1 cut(s) 115
LweI GCATC 2 cut(s) 81, 650
MaeII ACGT 2 cut(s) 365, 567
MaeIII GTNAC 5 cut(s) 10, 37, 517, 553, 608
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MboII GAAGA 3 cut(s) 43, 338, 669
MlsI TGGCCA 2 cut(s) 112, 181
MluCI AATT 4 cut(s) 187, 202, 413, 588
MluNI TGGCCA 2 cut(s) 112, 181
MlyI GAGTC 1 cut(s) 271
MnlI CCTC 1 cut(s) 73
Mox20I TGGCCA 2 cut(s) 112, 181
MroXI GAANNNNTTC 1 cut(s) 413
MscI TGGCCA 2 cut(s) 112, 181
MseI TTAA 4 cut(s) 201, 234, 483, 685
Msp20I TGGCCA 2 cut(s) 112, 181
MspA1I CMGCKG 1 cut(s) 443
MspI CCGG 2 cut(s) 55, 290
MspR9I CCNGG 1 cut(s) 462
Mva1269I GAATGC 1 cut(s) 507
MvaI CCWGG 1 cut(s) 462
MvnI CGCG 4 cut(s) 77, 379, 393, 488
MwoI GCNNNNNNNGC 3 cut(s) 78, 109, 583
NdeI CATATG 1 cut(s) 525
NdeII GATC 1 cut(s) 358
NlaIII CATG 3 cut(s) 173, 347, 623
NlaIV GGNNCC 4 cut(s) 226, 308, 410, 531
NmuCI GTSAC 1 cut(s) 10
NruI TCGCGA 1 cut(s) 77
PauI GCGCGC 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 321
PcsI WCGNNNNNNNCGW 1 cut(s) 362
PctI GAATGC 1 cut(s) 507
PdmI GAANNNNTTC 1 cut(s) 413
PkrI GCNGC 4 cut(s) 105, 229, 442, 665
PleI GAGTC 1 cut(s) 271
PpsI GAGTC 1 cut(s) 271
Psp1406I AACGTT 1 cut(s) 567
Psp6I CCWGG 1 cut(s) 460
PspGI CCWGG 1 cut(s) 460
PspN4I GGNNCC 4 cut(s) 226, 308, 410, 531
PspPI GGNCC 3 cut(s) 225, 292, 529
PteI GCGCGC 1 cut(s) 391
RruI TCGCGA 1 cut(s) 77
RsaI GTAC 1 cut(s) 643
RsaNI GTAC 1 cut(s) 642
SapI GCTCTTC 1 cut(s) 321
SaqAI TTAA 4 cut(s) 201, 234, 483, 685
SatI GCNGC 4 cut(s) 104, 228, 441, 664
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 3 cut(s) 225, 292, 529
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 1 cut(s) 462
SetI ASST 7 cut(s) 83, 276, 297, 320, 368, 570, 588
SfaNI GCATC 2 cut(s) 81, 650
SinI GGWCC 1 cut(s) 292
Sse9I AATT 4 cut(s) 187, 202, 413, 588
SsiI CCGC 4 cut(s) 228, 441, 458, 663
SspI AATATT 1 cut(s) 89
StyD4I CCNGG 1 cut(s) 460
TaaI ACNGT 2 cut(s) 131, 559
TaiI ACGT 2 cut(s) 368, 570
TaqI TCGA 2 cut(s) 399, 417
TasI AATT 4 cut(s) 187, 202, 413, 588
TauI GCSGC 3 cut(s) 230, 443, 666
Tru1I TTAA 4 cut(s) 201, 234, 483, 685
Tru9I TTAA 4 cut(s) 201, 234, 483, 685
TscAI CASTG 3 cut(s) 267, 338, 564
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 158, 609
TspRI CASTG 3 cut(s) 267, 338, 564
VpaK11BI GGWCC 1 cut(s) 292
XmnI GAANNNNTTC 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.