Prupe.8G219600_v2.0.a1

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000345
Physical Location & Seq
Reverse (-)
19985717 .. 19986844
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G219600.1

Sequence Viewer

Length: 606 bp
ATGAAATTCTTGGAGACCATAGTTTTCATGGTTTTAGGTATTGCTCTTTCCTTCAACATTGGGGGAAACATGAGCTGCAATGCTCCTGGACAGTATGACTACTTCCAGTTTGTTCAGGAGTCTCCTTTTCTATTTTGCAAAAATATTAATGGAAAGGGATGCAACATCCTACCTCTGCCACGGATATTTACTATTCATGGCTTGTGGCCAAGCAACTTCACACATCGTCATAAACCTTGCGTTGGTGCACAATTTAGTAGAGATATAAATGAAGCCTCCAATGATGAATTGCGAGCTGATCTGGAGTTGTCATGGCGAAGCTTCATCAGTGGGCGGTCAAATATGGACTTCTGGGAATATGAATACAACAAACACGGCAAGTGCTCCGACGACAAGTTTTCGCAGACGCAATACTTCGACCGTGCCAGGAGTTTGTGGAAGCAATACAAACCTCATGCTCTATTTTCTAATCGCTCGCTTGAACCAGGCAAGTCATATAGTTTTACTGGCCTTGAACAAGCCATCCGATCATTTATAGGCGGGAGCAGACCTCTTCTCCTTTGCGAGCAATCTAAGAGGAAGAAGAACAAGAAGAAGACAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

202

Amino Acids

23.29

Weight (kDa)

9.47

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 334, 540
AcoI YGGCCR 1 cut(s) 206
AcsI RAATTY 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 3 cut(s) 55, 482, 515
AjnI CCWGG 3 cut(s) 85, 425, 484
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
AluBI AGCT 3 cut(s) 75, 296, 321
AluI AGCT 3 cut(s) 75, 296, 321
Alw21I GWGCWC 2 cut(s) 250, 386
Alw26I GTCTC 2 cut(s) 8, 126
Alw44I GTGCAC 1 cut(s) 246
AoxI GGCC 2 cut(s) 206, 508
ApaLI GTGCAC 1 cut(s) 246
ApeKI GCWGC 1 cut(s) 75
ApoI RAATTY 1 cut(s) 5
AseI ATTAAT 1 cut(s) 147
BaeGI GKGCMC 1 cut(s) 250
BalI TGGCCA 1 cut(s) 208
BarI GAAGNNNNNNTAC 2 cut(s) 86, 118
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 2 cut(s) 250, 386
BbvI GCAGC 1 cut(s) 62
BccI CCATC 1 cut(s) 530
BceAI ACGGC 1 cut(s) 391
BciT130I CCWGG 3 cut(s) 87, 427, 486
BcoDI GTCTC 2 cut(s) 8, 126
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 3 cut(s) 87, 427, 486
BmrFI CCNGG 3 cut(s) 87, 427, 486
BmsI GCATC 1 cut(s) 149
BpiI GAAGAC 1 cut(s) 602
BplI GAGNNNNNCTC 2 cut(s) 535, 567
BpmI CTGGAG 1 cut(s) 323
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bsc4I CCNNNNNNNGG 1 cut(s) 242
Bse1I ACTGG 2 cut(s) 106, 511
Bse3DI GCAATG 1 cut(s) 85
BseBI CCWGG 3 cut(s) 87, 427, 486
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 3 cut(s) 164, 165, 522
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMI GCAATG 1 cut(s) 85
BseNI ACTGG 2 cut(s) 106, 511
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 421
BshFI GGCC 2 cut(s) 208, 510
BsiEI CGRYCG 1 cut(s) 421
BsiHKAI GWGCWC 2 cut(s) 250, 386
BslI CCNNNNNNNGG 1 cut(s) 242
BsmAI GTCTC 2 cut(s) 8, 126
BsnI GGCC 2 cut(s) 208, 510
Bso31I GGTCTC 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 250, 386
Bsp143I GATC 2 cut(s) 298, 527
BspACI CCGC 2 cut(s) 334, 540
BspANI GGCC 2 cut(s) 208, 510
BspTNI GGTCTC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 85
BsrI ACTGG 2 cut(s) 106, 511
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 2 cut(s) 298, 527
Bst2UI CCWGG 3 cut(s) 87, 427, 486
Bst4CI ACNGT 2 cut(s) 93, 422
Bst6I CTCTTC 1 cut(s) 558
BstC8I GCNNGC 3 cut(s) 294, 476, 566
BstDEI CTNAG 1 cut(s) 573
BstDSI CCRYGG 1 cut(s) 179
BstF5I GGATG 3 cut(s) 164, 165, 522
BstKTI GATC 2 cut(s) 301, 530
BstMAI GTCTC 2 cut(s) 8, 126
BstMBI GATC 2 cut(s) 298, 527
BstMCI CGRYCG 1 cut(s) 421
BstNI CCWGG 3 cut(s) 87, 427, 486
BstSCI CCNGG 3 cut(s) 85, 425, 484
BstSLI GKGCMC 1 cut(s) 250
BstV1I GCAGC 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 602
BsuRI GGCC 2 cut(s) 208, 510
BtgI CCRYGG 1 cut(s) 179
BtsCI GGATG 3 cut(s) 164, 165, 522
BtsIMutI CAGTG 1 cut(s) 334
Cac8I GCNNGC 3 cut(s) 294, 476, 566
CseI GACGC 1 cut(s) 415
CviAII CATG 5 cut(s) 28, 70, 197, 312, 455
CviJI RGCY 8 cut(s) 75, 201, 208, 275, 296, 321, 510, 521
CviKI_1 RGCY 8 cut(s) 75, 201, 208, 275, 296, 321, 510, 521
DdeI CTNAG 1 cut(s) 573
DpnI GATC 2 cut(s) 300, 529
DpnII GATC 2 cut(s) 298, 527
EaeI YGGCCR 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Eco31I GGTCTC 1 cut(s) 8
EcoRII CCWGG 3 cut(s) 85, 425, 484
FaeI CATG 5 cut(s) 31, 73, 200, 315, 458
FatI CATG 5 cut(s) 27, 69, 196, 311, 454
FauI CCCGC 1 cut(s) 533
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 3 cut(s) 152, 171, 509
Fsp4HI GCNGC 1 cut(s) 76
GluI GCNGC 1 cut(s) 76
GsuI CTGGAG 1 cut(s) 323
HaeIII GGCC 2 cut(s) 208, 510
HgaI GACGC 1 cut(s) 415
Hin1II CATG 5 cut(s) 31, 73, 200, 315, 458
HindIII AAGCTT 1 cut(s) 319
HinfI GANTC 1 cut(s) 119
Hpy166II GTNNAC 1 cut(s) 248
Hpy188I TCNGA 2 cut(s) 388, 527
Hpy188III TCNNGA 2 cut(s) 116, 302
Hpy8I GTNNAC 1 cut(s) 248
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 2 cut(s) 93, 422
HpyCH4V TGCA 4 cut(s) 78, 138, 162, 248
HpyF3I CTNAG 1 cut(s) 573
Hsp92II CATG 5 cut(s) 31, 73, 200, 315, 458
Kzo9I GATC 2 cut(s) 298, 527
LmnI GCTCC 3 cut(s) 88, 389, 543
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 149
MalI GATC 2 cut(s) 300, 529
MboI GATC 2 cut(s) 298, 527
MboII GAAGA 4 cut(s) 545, 592, 595, 604
MhlI GDGCHC 2 cut(s) 250, 386
MlsI TGGCCA 1 cut(s) 208
MluCI AATT 3 cut(s) 5, 251, 287
MluNI TGGCCA 1 cut(s) 208
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 5 cut(s) 183, 286, 462, 561, 570
Mox20I TGGCCA 1 cut(s) 208
MscI TGGCCA 1 cut(s) 208
MseI TTAA 1 cut(s) 147
Msp20I TGGCCA 1 cut(s) 208
MspR9I CCNGG 3 cut(s) 87, 427, 486
MvaI CCWGG 3 cut(s) 87, 427, 486
NdeII GATC 2 cut(s) 298, 527
NlaIII CATG 5 cut(s) 31, 73, 200, 315, 458
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PshBI ATTAAT 1 cut(s) 147
Psp6I CCWGG 3 cut(s) 85, 425, 484
PspGI CCWGG 3 cut(s) 85, 425, 484
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 2 cut(s) 298, 527
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 3 cut(s) 87, 427, 486
SduI GDGCHC 2 cut(s) 250, 386
SetI ASST 8 cut(s) 40, 77, 175, 238, 298, 323, 454, 553
SfaNI GCATC 1 cut(s) 149
Sse9I AATT 3 cut(s) 5, 251, 287
SsiI CCGC 2 cut(s) 334, 540
SspI AATATT 1 cut(s) 145
StyD4I CCNGG 3 cut(s) 85, 425, 484
TaaI ACNGT 2 cut(s) 93, 422
TaqI TCGA 1 cut(s) 417
TasI AATT 3 cut(s) 5, 251, 287
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 1 cut(s) 334
TseI GCWGC 1 cut(s) 75
TspDTI ATGAA 7 cut(s) 16, 17, 185, 285, 300, 313, 375
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 1 cut(s) 334
VneI GTGCAC 1 cut(s) 246
VspI ATTAAT 1 cut(s) 147
XapI RAATTY 1 cut(s) 5
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.