Rmu_sc0008973.1_g000002

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008973.1
OG0000345
Physical Location & Seq
Reverse (-)
7851 .. 10391
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008973.1_g000002.1.cds

Sequence Viewer

Length: 651 bp
atgtccgcgaactaccgtcttcaaaccatattgctcccatcctcgtggagaagctaccttatcgctgtctccaacacaaacttctggcagactgagtataacaaacatggtacatgttcggagctcaatttgccacagacggaatacttcaccacaacccattggctatggatggtcaacaacatttatagtatattttcatcttcggtaaacatacaaggatatccaatcttgccgggtttcatctatgactacaccgatcttcttgccgctgttcaactaggaatcggtggcgtcactcccgtgcttacttgcatggtcataaataaccactacgttcttcacgaagtggtcggtcagacggtgcagccgcagaagacgaagaagaagaagagcatcgagaccaaggctaccgactccacggctgtgaccatcgaccagacgacgtcgtccaccacgatcgacaggctcagctccggcaaccaactccggggggtccaccccatcgatgaccggaatgatgacgtgaaccgccaccaggacatttctaagcttctagtctcgacttcgagtaggttttgtaaaagagaggggggcgggtggcagcccggctggtttggtacgtatttatatagggtaggaagatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

216

Amino Acids

24.52

Weight (kDa)

8.72

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 448
AatII GACGTC 1 cut(s) 449
AccII CGCG 1 cut(s) 8
AciI CCGC 5 cut(s) 6, 270, 371, 532, 597
AcyI GRCGYC 2 cut(s) 294, 446
AdeI CACNNNGTG 1 cut(s) 349
AfaI GTAC 2 cut(s) 112, 622
AfiI CCNNNNNNNGG 2 cut(s) 490, 538
AflIII ACRYGT 1 cut(s) 113
AgsI TTSAA 2 cut(s) 23, 278
AjiI CACGTC 1 cut(s) 526
AjnI CCWGG 1 cut(s) 537
AjuI GAANNNNNNNTTGG 2 cut(s) 65, 97
AleI CACNNNNGTG 2 cut(s) 302, 425
AluBI AGCT 4 cut(s) 54, 124, 474, 553
AluI AGCT 4 cut(s) 54, 124, 474, 553
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 3 cut(s) 73, 395, 565
ApeKI GCWGC 2 cut(s) 367, 604
AspS9I GGNCC 1 cut(s) 496
AsuC2I CCSGG 3 cut(s) 237, 491, 609
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 1 cut(s) 496
BanII GRGCYC 1 cut(s) 126
BauI CACGAG 1 cut(s) 43
BbsI GAAGAC 2 cut(s) 11, 383
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 379, 616
BccI CCATC 5 cut(s) 46, 166, 440, 512, 639
BceAI ACGGC 1 cut(s) 438
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 1 cut(s) 539
BcnI CCSGG 3 cut(s) 237, 491, 609
BcoDI GTCTC 3 cut(s) 73, 395, 565
BfaI CTAG 2 cut(s) 281, 557
BisI GCNGC 4 cut(s) 270, 368, 371, 605
BlpI GCTNAGC 1 cut(s) 470
BlsI GCNGC 4 cut(s) 271, 369, 372, 606
Bme1390I CCNGG 4 cut(s) 237, 491, 539, 609
Bme18I GGWCC 1 cut(s) 496
BmgBI CACGTC 1 cut(s) 526
BmgT120I GGNCC 1 cut(s) 496
BmiI GGNNCC 1 cut(s) 497
BmrFI CCNGG 4 cut(s) 237, 491, 539, 609
BmsI GCATC 1 cut(s) 405
BpiI GAAGAC 2 cut(s) 11, 383
Bpu1102I GCTNAGC 1 cut(s) 470
BpuMI CCSGG 3 cut(s) 237, 491, 609
Bsa29I ATCGAT 1 cut(s) 507
BsaAI YACGTR 1 cut(s) 624
BsaHI GRCGYC 2 cut(s) 294, 446
BsaI GGTCTC 1 cut(s) 395
BsaJI CCNNGG 3 cut(s) 405, 420, 490
BsaWI WCCGGW 1 cut(s) 513
Bsc4I CCNNNNNNNGG 2 cut(s) 490, 538
BseBI CCWGG 1 cut(s) 539
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 3 cut(s) 405, 420, 490
BseGI GGATG 2 cut(s) 38, 177
BseLI CCNNNNNNNGG 2 cut(s) 490, 538
BseMII CTCAG 2 cut(s) 84, 484
BseXI GCAGC 2 cut(s) 379, 616
BsgI GTGCAG 1 cut(s) 386
Bsh1236I CGCG 1 cut(s) 8
Bsh1285I CGRYCG 1 cut(s) 462
BshVI ATCGAT 1 cut(s) 507
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 5 cut(s) 236, 477, 490, 514, 609
BslI CCNNNNNNNGG 2 cut(s) 490, 538
BsmAI GTCTC 3 cut(s) 73, 395, 565
Bso31I GGTCTC 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 259, 459
Bsp1720I GCTNAGC 1 cut(s) 470
BspACI CCGC 5 cut(s) 6, 270, 371, 532, 597
BspCNI CTCAG 2 cut(s) 85, 483
BspDI ATCGAT 1 cut(s) 507
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 497
BspQI GCTCTTC 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 395
BssECI CCNNGG 3 cut(s) 405, 420, 490
BssMI GATC 2 cut(s) 259, 459
BssNI GRCGYC 2 cut(s) 294, 446
BssSI CACGAG 1 cut(s) 43
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 43
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 2 cut(s) 17, 364
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 2 cut(s) 294, 446
BstBAI YACGTR 1 cut(s) 624
BstDEI CTNAG 3 cut(s) 93, 470, 549
BstDSI CCRYGG 1 cut(s) 420
BstF5I GGATG 2 cut(s) 38, 177
BstFNI CGCG 1 cut(s) 8
BstKTI GATC 2 cut(s) 262, 462
BstMAI GTCTC 3 cut(s) 73, 395, 565
BstMBI GATC 2 cut(s) 259, 459
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 1 cut(s) 539
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 4 cut(s) 235, 489, 537, 607
BstSNI TACGTA 1 cut(s) 624
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 2 cut(s) 379, 616
BstV2I GAAGAC 2 cut(s) 11, 383
BstXI CCANNNNNNTGG 1 cut(s) 45
Bsu15I ATCGAT 1 cut(s) 507
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 1 cut(s) 420
BtrI CACGTC 1 cut(s) 526
BtsCI GGATG 2 cut(s) 38, 177
Cfr13I GGNCC 1 cut(s) 496
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 1 cut(s) 283
Csp6I GTAC 2 cut(s) 111, 621
CviAII CATG 3 cut(s) 107, 114, 316
CviQI GTAC 2 cut(s) 111, 621
DdeI CTNAG 3 cut(s) 93, 470, 549
DpnI GATC 2 cut(s) 261, 461
DpnII GATC 2 cut(s) 259, 459
DraIII CACNNNGTG 1 cut(s) 349
DrdI GACNNNNNNGTC 1 cut(s) 448
DseDI GACNNNNNNGTC 1 cut(s) 448
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Ecl136II GAGCTC 1 cut(s) 124
Eco105I TACGTA 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 405
Eco24I GRGCYC 1 cut(s) 126
Eco31I GGTCTC 1 cut(s) 395
Eco32I GATATC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 537
EcoRV GATATC 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 405
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 3 cut(s) 110, 117, 319
FatI CATG 3 cut(s) 106, 113, 315
FauI CCCGC 1 cut(s) 590
Fnu4HI GCNGC 4 cut(s) 270, 368, 371, 605
FokI GGATG 2 cut(s) 25, 184
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 4 cut(s) 270, 368, 371, 605
FspBI CTAG 2 cut(s) 281, 557
GluI GCNGC 4 cut(s) 270, 368, 371, 605
HapII CCGG 5 cut(s) 236, 477, 490, 514, 609
HgaI GACGC 1 cut(s) 283
Hin1I GRCGYC 2 cut(s) 294, 446
Hin1II CATG 3 cut(s) 110, 117, 319
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 2 cut(s) 285, 416
HpaII CCGG 5 cut(s) 236, 477, 490, 514, 609
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 5 cut(s) 178, 211, 453, 499, 529
Hpy188I TCNGA 2 cut(s) 121, 360
Hpy188III TCNNGA 3 cut(s) 344, 400, 562
Hpy8I GTNNAC 5 cut(s) 178, 211, 453, 499, 529
Hpy99I CGWCG 2 cut(s) 448, 451
HpyCH4III ACNGT 2 cut(s) 17, 364
HpyCH4IV ACGT 4 cut(s) 336, 446, 525, 623
HpyCH4V TGCA 2 cut(s) 315, 367
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 3 cut(s) 93, 470, 549
HpySE526I ACGT 4 cut(s) 336, 446, 525, 623
Hsp92I GRCGYC 2 cut(s) 294, 446
Hsp92II CATG 3 cut(s) 110, 117, 319
Kzo9I GATC 2 cut(s) 259, 459
LguI GCTCTTC 1 cut(s) 386
LmnI GCTCC 3 cut(s) 39, 121, 479
Lsp1109I GCAGC 2 cut(s) 379, 616
LweI GCATC 1 cut(s) 405
MaeI CTAG 2 cut(s) 281, 557
MaeII ACGT 4 cut(s) 336, 446, 525, 623
MaeIII GTNAC 2 cut(s) 295, 427
MalI GATC 2 cut(s) 261, 461
MboI GATC 2 cut(s) 259, 459
MboII GAAGA 9 cut(s) 11, 195, 254, 332, 388, 394, 397, 400, 403
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 1 cut(s) 127
MlyI GAGTC 1 cut(s) 410
MmeI TCCRAC 1 cut(s) 96
MnlI CCTC 2 cut(s) 52, 583
MslI CAYNNNNRTG 3 cut(s) 43, 302, 425
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 5 cut(s) 236, 477, 490, 514, 609
MspR9I CCNGG 4 cut(s) 237, 491, 539, 609
MvaI CCWGG 1 cut(s) 539
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 1 cut(s) 130
NciI CCSGG 3 cut(s) 237, 491, 609
NdeII GATC 2 cut(s) 259, 459
NlaIII CATG 3 cut(s) 110, 117, 319
NlaIV GGNNCC 1 cut(s) 497
NmuCI GTSAC 2 cut(s) 295, 427
NspI RCATGY 1 cut(s) 117
OliI CACNNNNGTG 2 cut(s) 302, 425
PciI ACATGT 1 cut(s) 113
PciSI GCTCTTC 1 cut(s) 386
PcsI WCGNNNNNNNCGW 2 cut(s) 342, 455
PfeI GAWTC 1 cut(s) 285
PflFI GACNNNGTC 2 cut(s) 445, 448
PkrI GCNGC 4 cut(s) 271, 369, 372, 606
Ple19I CGATCG 1 cut(s) 462
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
Ppu21I YACGTR 1 cut(s) 624
PscI ACATGT 1 cut(s) 113
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 1 cut(s) 497
PspPI GGNCC 1 cut(s) 496
PsyI GACNNNGTC 2 cut(s) 445, 448
PvuI CGATCG 1 cut(s) 462
RsaI GTAC 2 cut(s) 112, 622
RsaNI GTAC 2 cut(s) 111, 621
RseI CAYNNNNRTG 3 cut(s) 43, 302, 425
SacI GAGCTC 1 cut(s) 126
SapI GCTCTTC 1 cut(s) 386
SatI GCNGC 4 cut(s) 270, 368, 371, 605
Sau3AI GATC 2 cut(s) 259, 459
Sau96I GGNCC 1 cut(s) 496
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 4 cut(s) 237, 491, 539, 609
SduI GDGCHC 1 cut(s) 126
SfaNI GCATC 1 cut(s) 405
SinI GGWCC 1 cut(s) 496
SmiMI CAYNNNNRTG 3 cut(s) 43, 302, 425
SnaBI TACGTA 1 cut(s) 624
Sse9I AATT 1 cut(s) 127
SsiI CCGC 5 cut(s) 6, 270, 371, 532, 597
SspMI CTAG 2 cut(s) 281, 557
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 4 cut(s) 235, 489, 537, 607
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 2 cut(s) 17, 364
TaiI ACGT 4 cut(s) 339, 449, 528, 626
TaqI TCGA 6 cut(s) 399, 435, 462, 507, 563, 569
TaqII GACCGA 1 cut(s) 344
TasI AATT 1 cut(s) 127
TauI GCSGC 2 cut(s) 272, 373
TfiI GAWTC 1 cut(s) 285
TseFI GTSAC 2 cut(s) 295, 427
TseI GCWGC 2 cut(s) 367, 604
Tsp45I GTSAC 2 cut(s) 295, 427
TspDTI ATGAA 2 cut(s) 189, 232
TspGWI ACGGA 1 cut(s) 155
Tth111I GACNNNGTC 2 cut(s) 445, 448
VpaK11BI GGWCC 1 cut(s) 496
XceI RCATGY 1 cut(s) 117
XspI CTAG 2 cut(s) 281, 557
ZraI GACGTC 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.