Rh2DG224200

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000345
Physical Location & Seq
Forward (+)
21759415 .. 21769533
10119 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG224200.1

Sequence Viewer

Length: 939 bp
ATGGGTGCGGTGGGGCTGCGGTGGCTGACAATGACGGGGTTGGTATATGCACTTGTACAAGTTCTCAACGTTTCAAATGCTGAACCATACGAGTACATGCAATTTGTACTCCAGTACCCACCTGGAGTTTGCTATGACAGATCAAAAGGTTATTGCATCTTACCTCTGCCAACCAAGTTTCATGTACATGGAATATGGCCTTCCAACTTCTCCGATATAGATGTCACTTGTGGTCAAGCACTGACGAATAACCCCTTTGATAACGCGCAGATGACACCTCTGCAGACGGATTTAGTAAACTCATGGCCGTCCGTGTTAACAACAAAGAGCAATATGTGGTTCTGGGAGCATGAGTACGAGAACCATGGCGCATGTACAGTAGAGTCAGGTGTACCACCTTTCACTCAAAAGTCCTACTTTAAGAAAGGACACCAGCTATGGAACCAATATGACATCCATTCAGTGCTTGATCAAAGTGGTATTAAACCGAGTACTGCAAAATCATACACTATGACTCAGCTTGTAACCGCAATCAAAAAGAAAATCGGGAGTAACAATACCCCTTTAATCATGTGCAGGAAGAAAAAACTTGGTTACATACTAAGGGAAGTGATAATCTGTCTGGATCACCAGGCAACAAATGTGATAAGTTGCGCGCTCAGTAAAAGGAAAACAGACTACCTGGATCCTTCGGGCAAGCTAAGGCATCAAGCACATCTTTCTGTTTTGGACAACACAGCTGAAGTTCAGCCATATATAGATGAGCATTTGGAGAGATTGAAACTGGAGCACCCTCAAAAGTCTAAGGCAGAAAATTGGCTTAAAGATGAACATAATCGAAGATTTAGCAATTGGCTGCAACAAAGAGTATGTAGAATAGGGCAGATTCTTCTTCATTCTAGCATTATTGTTTATCTTGTAATAATGTTCAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

312

Amino Acids

35.84

Weight (kDa)

8.88

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 30 - 218 4.9e-40 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 266, 656
AciI CCGC 3 cut(s) 8, 19, 528
AclI AACGTT 1 cut(s) 69
AclWI GGATC 3 cut(s) 633, 680, 693
AcoI YGGCCR 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 762
AfaI GTAC 9 cut(s) 57, 95, 108, 116, 186, 356, 376, 393, 493
AgsI TTSAA 3 cut(s) 75, 781, 931
AjnI CCWGG 3 cut(s) 121, 630, 681
AluBI AGCT 4 cut(s) 436, 520, 700, 740
AluI AGCT 4 cut(s) 436, 520, 700, 740
Alw21I GWGCWC 1 cut(s) 792
AlwI GGATC 3 cut(s) 633, 680, 693
AoxI GGCC 2 cut(s) 197, 305
ApeKI GCWGC 2 cut(s) 16, 856
AspLEI GCGC 4 cut(s) 268, 371, 656, 658
AsuHPI GGTGA 1 cut(s) 620
BaeI ACNNNNGTAYC 2 cut(s) 98, 131
BamHI GGATCC 1 cut(s) 685
Bbv12I GWGCWC 1 cut(s) 792
BbvI GCAGC 2 cut(s) 3, 843
BceAI ACGGC 1 cut(s) 292
BciT130I CCWGG 3 cut(s) 123, 632, 683
BclI TGATCA 1 cut(s) 469
BfaI CTAG 1 cut(s) 900
BfmI CTRYAG 1 cut(s) 281
BisI GCNGC 2 cut(s) 17, 857
BlsI GCNGC 2 cut(s) 18, 858
BmcAI AGTACT 1 cut(s) 493
Bme1390I CCNGG 3 cut(s) 123, 632, 683
BmiI GGNNCC 2 cut(s) 443, 687
BmrFI CCNGG 3 cut(s) 123, 632, 683
BmsI GCATC 2 cut(s) 165, 715
BpmI CTGGAG 3 cut(s) 95, 144, 806
Bpu10I CCTNAGC 1 cut(s) 701
BsaJI CCNNGG 1 cut(s) 364
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bse1I ACTGG 2 cut(s) 112, 789
BseBI CCWGG 3 cut(s) 123, 632, 683
BseDI CCNNGG 1 cut(s) 364
BseGI GGATG 1 cut(s) 453
BseMII CTCAG 2 cut(s) 530, 673
BseNI ACTGG 2 cut(s) 112, 789
BsePI GCGCGC 1 cut(s) 654
BseXI GCAGC 2 cut(s) 3, 843
BsgI GTGCAG 1 cut(s) 595
Bsh1236I CGCG 2 cut(s) 266, 656
BshFI GGCC 2 cut(s) 199, 307
BsiHKAI GWGCWC 1 cut(s) 792
BsnI GGCC 2 cut(s) 199, 307
Bsp1286I GDGCHC 1 cut(s) 792
Bsp1407I TGTACA 3 cut(s) 55, 184, 374
Bsp143I GATC 4 cut(s) 140, 469, 625, 685
Bsp19I CCATGG 1 cut(s) 364
BspACI CCGC 3 cut(s) 8, 19, 528
BspANI GGCC 2 cut(s) 199, 307
BspCNI CTCAG 2 cut(s) 529, 672
BspFNI CGCG 2 cut(s) 266, 656
BspLI GGNNCC 2 cut(s) 443, 687
BspMAI CTGCAG 1 cut(s) 285
BspPI GGATC 3 cut(s) 633, 680, 693
BsrGI TGTACA 3 cut(s) 55, 184, 374
BsrI ACTGG 2 cut(s) 112, 789
BssECI CCNNGG 1 cut(s) 364
BssHII GCGCGC 1 cut(s) 654
BssMI GATC 4 cut(s) 140, 469, 625, 685
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 3 cut(s) 123, 632, 683
Bst4CI ACNGT 1 cut(s) 379
BstAUI TGTACA 3 cut(s) 55, 184, 374
BstC8I GCNNGC 2 cut(s) 656, 698
BstDEI CTNAG 5 cut(s) 516, 602, 659, 701, 804
BstDSI CCRYGG 1 cut(s) 364
BstF5I GGATG 1 cut(s) 453
BstFNI CGCG 2 cut(s) 266, 656
BstHHI GCGC 4 cut(s) 268, 371, 656, 658
BstKTI GATC 4 cut(s) 143, 472, 628, 688
BstMBI GATC 4 cut(s) 140, 469, 625, 685
BstMWI GCNNNNNNNGC 1 cut(s) 22
BstNI CCWGG 3 cut(s) 123, 632, 683
BstNSI RCATGY 2 cut(s) 100, 375
BstSCI CCNGG 3 cut(s) 121, 630, 681
BstSFI CTRYAG 1 cut(s) 281
BstUI CGCG 2 cut(s) 266, 656
BstV1I GCAGC 2 cut(s) 3, 843
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BsuRI GGCC 2 cut(s) 199, 307
BtgI CCRYGG 1 cut(s) 364
BtsCI GGATG 1 cut(s) 453
BtsIMutI CAGTG 2 cut(s) 239, 468
Cac8I GCNNGC 2 cut(s) 656, 698
CfoI GCGC 4 cut(s) 268, 371, 656, 658
Csp6I GTAC 9 cut(s) 56, 94, 107, 115, 185, 355, 375, 392, 492
CviAII CATG 8 cut(s) 97, 182, 188, 303, 350, 365, 372, 571
CviQI GTAC 9 cut(s) 56, 94, 107, 115, 185, 355, 375, 392, 492
DdeI CTNAG 5 cut(s) 516, 602, 659, 701, 804
DpnI GATC 4 cut(s) 142, 471, 627, 687
DpnII GATC 4 cut(s) 140, 469, 625, 685
EaeI YGGCCR 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 762
EcoRII CCWGG 3 cut(s) 121, 630, 681
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 8 cut(s) 100, 185, 191, 306, 353, 368, 375, 574
FalI AAGNNNNNCTT 4 cut(s) 401, 433, 702, 734
FatI CATG 8 cut(s) 96, 181, 187, 302, 349, 364, 371, 570
FbaI TGATCA 1 cut(s) 469
Fnu4HI GCNGC 2 cut(s) 17, 857
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 2 cut(s) 17, 857
FspBI CTAG 1 cut(s) 900
GlaI GCGC 4 cut(s) 267, 370, 655, 657
GluI GCNGC 2 cut(s) 17, 857
GsuI CTGGAG 3 cut(s) 95, 144, 806
HaeIII GGCC 2 cut(s) 199, 307
HhaI GCGC 4 cut(s) 268, 371, 656, 658
Hin1II CATG 8 cut(s) 100, 185, 191, 306, 353, 368, 375, 574
Hin6I GCGC 4 cut(s) 266, 369, 654, 656
HinP1I GCGC 4 cut(s) 266, 369, 654, 656
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HinfI GANTC 3 cut(s) 383, 514, 886
HpaI GTTAAC 1 cut(s) 318
HphI GGTGA 1 cut(s) 620
Hpy166II GTNNAC 3 cut(s) 298, 318, 392
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 2 cut(s) 547, 623
Hpy8I GTNNAC 3 cut(s) 298, 318, 392
HpyAV CCTTC 2 cut(s) 210, 699
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 7 cut(s) 50, 100, 156, 283, 497, 576, 859
HpyF10VI GCNNNNNNNGC 1 cut(s) 22
HpyF3I CTNAG 5 cut(s) 516, 602, 659, 701, 804
HpySE526I ACGT 1 cut(s) 69
Hsp92II CATG 8 cut(s) 100, 185, 191, 306, 353, 368, 375, 574
HspAI GCGC 4 cut(s) 266, 369, 654, 656
Ksp22I TGATCA 1 cut(s) 469
KspAI GTTAAC 1 cut(s) 318
Kzo9I GATC 4 cut(s) 140, 469, 625, 685
LmnI GCTCC 2 cut(s) 346, 787
Lsp1109I GCAGC 2 cut(s) 3, 843
LweI GCATC 2 cut(s) 165, 715
MaeI CTAG 1 cut(s) 900
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 4 cut(s) 223, 523, 551, 593
MalI GATC 4 cut(s) 142, 471, 627, 687
MboI GATC 4 cut(s) 140, 469, 625, 685
MboII GAAGA 4 cut(s) 592, 852, 881, 884
MfeI CAATTG 1 cut(s) 850
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 1 cut(s) 792
MluCI AATT 3 cut(s) 101, 814, 850
MlyI GAGTC 2 cut(s) 392, 508
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 3 cut(s) 174, 288, 804
MseI TTAA 6 cut(s) 317, 420, 483, 566, 822, 937
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 740
MspR9I CCNGG 3 cut(s) 123, 632, 683
MunI CAATTG 1 cut(s) 850
MvaI CCWGG 3 cut(s) 123, 632, 683
MvnI CGCG 2 cut(s) 266, 656
MwoI GCNNNNNNNGC 1 cut(s) 22
NcoI CCATGG 1 cut(s) 364
NdeII GATC 4 cut(s) 140, 469, 625, 685
NlaIII CATG 8 cut(s) 100, 185, 191, 306, 353, 368, 375, 574
NlaIV GGNNCC 2 cut(s) 443, 687
NmuCI GTSAC 1 cut(s) 223
NspI RCATGY 2 cut(s) 100, 375
PauI GCGCGC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 886
PkrI GCNGC 2 cut(s) 18, 858
PleI GAGTC 2 cut(s) 391, 508
PpsI GAGTC 2 cut(s) 391, 508
Psp1406I AACGTT 1 cut(s) 69
Psp6I CCWGG 3 cut(s) 121, 630, 681
PspGI CCWGG 3 cut(s) 121, 630, 681
PspN4I GGNNCC 2 cut(s) 443, 687
PstI CTGCAG 1 cut(s) 285
PsuI RGATCY 1 cut(s) 685
PteI GCGCGC 1 cut(s) 654
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 9 cut(s) 57, 95, 108, 116, 186, 356, 376, 393, 493
RsaNI GTAC 9 cut(s) 56, 94, 107, 115, 185, 355, 375, 392, 492
RseI CAYNNNNRTG 1 cut(s) 186
SaqAI TTAA 6 cut(s) 317, 420, 483, 566, 822, 937
SatI GCNGC 2 cut(s) 17, 857
Sau3AI GATC 4 cut(s) 140, 469, 625, 685
ScaI AGTACT 1 cut(s) 493
SchI GAGTC 2 cut(s) 392, 508
ScrFI CCNGG 3 cut(s) 123, 632, 683
SduI GDGCHC 1 cut(s) 792
SfaNI GCATC 2 cut(s) 165, 715
SfcI CTRYAG 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 186
Sse9I AATT 3 cut(s) 101, 814, 850
SsiI CCGC 3 cut(s) 8, 19, 528
SspMI CTAG 1 cut(s) 900
StyD4I CCNGG 3 cut(s) 121, 630, 681
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 72
TaqI TCGA 1 cut(s) 838
TasI AATT 3 cut(s) 101, 814, 850
TatI WGTACW 6 cut(s) 55, 93, 106, 184, 374, 491
TfiI GAWTC 1 cut(s) 886
Tru1I TTAA 6 cut(s) 317, 420, 483, 566, 822, 937
Tru9I TTAA 6 cut(s) 317, 420, 483, 566, 822, 937
TscAI CASTG 2 cut(s) 246, 468
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 2 cut(s) 16, 856
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 3 cut(s) 170, 843, 884
TspGWI ACGGA 2 cut(s) 301, 302
TspRI CASTG 2 cut(s) 246, 468
XceI RCATGY 2 cut(s) 100, 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 119
XspI CTAG 1 cut(s) 900
ZrmI AGTACT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.