RchiOBHm_Chr6g0293901

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000345
Physical Location & Seq
Forward (+)
56433051 .. 56434070
1020 bp
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UTR
Exon/CDS
Intron
PRQ26374

Sequence Viewer

Length: 678 bp
ATGACGATCCTGAATGGGGTGGCTCTTGCATTTGTTCTTTCCTTGTGTATCCCCATGTGCACCGGTTCATATGATCATTTTAAATTTGTACAACAATGGCCACCGGCCGTCTGTGATACGTCTGGCTGCAAGAGATCGGGGTTATCACGCTTCACGATCCATGGCCTTTGGCCAAATAATAAAACGTATGTGAAAAATCAACCGACTTGTCTTACCAATCGGGCCAACTCAATTAAGGCTACGATACTCACTAGTTCGAATTTGGTAAGCAAGCTGAGCACCTCTTGGCCCGATGTGAAAAATGCAAATGATAATGATTTTTGGAACAAACAATGGGACAAGCATGGCACATGTTCCTTGCAGACATACGACCAAGCACAATACTTCGAGCGGTCATACAACATGTGGAAAGAAACCAACCTGACAAAAACCTTGGATAGCCTCATCAAACAGACACCGAGACAACAGAACGTCACAGATATAGAACAACTCATTCAAGGAGTAACTGCAACTGAAAAGAAACCCCTCCTCCGTTGTGAACAATCTCATAGTAATGCCAGTAAGTCTCTCTTGAAAGAGATTGTCATTTGCTATGCACACAACGGGATTACTGTGATCGATTGCGTCAGTCGACCAAAATCCACCTTCGAATGCTATGACGGATTTTGGTTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

225

Amino Acids

25.59

Weight (kDa)

8.77

Isoelectric Point (pI)

41.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 24 - 208 1e-36 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 391
AccI GTMKAC 1 cut(s) 631
AciI CCGC 1 cut(s) 391
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 3 cut(s) 98, 105, 170
AcsI RAATTY 2 cut(s) 83, 259
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 16
AflIII ACRYGT 2 cut(s) 350, 402
AgeI ACCGGT 1 cut(s) 62
AgsI TTSAA 2 cut(s) 497, 574
AhlI ACTAGT 1 cut(s) 251
AluBI AGCT 1 cut(s) 274
AluI AGCT 1 cut(s) 274
Alw21I GWGCWC 2 cut(s) 62, 281
Alw26I GTCTC 2 cut(s) 454, 570
Alw44I GTGCAC 1 cut(s) 58
AlwI GGATC 1 cut(s) 151
AoxI GGCC 6 cut(s) 98, 105, 163, 170, 222, 287
ApaLI GTGCAC 1 cut(s) 58
ApeKI GCWGC 1 cut(s) 126
ApoI RAATTY 2 cut(s) 83, 259
AsiGI ACCGGT 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 222, 288
AsuII TTCGAA 2 cut(s) 257, 648
BaeGI GKGCMC 1 cut(s) 62
BalI TGGCCA 2 cut(s) 100, 172
Bbv12I GWGCWC 2 cut(s) 62, 281
BbvI GCAGC 1 cut(s) 113
BceAI ACGGC 1 cut(s) 92
BciVI GTATCC 1 cut(s) 59
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 2 cut(s) 454, 570
BcuI ACTAGT 1 cut(s) 251
BfaI CTAG 1 cut(s) 252
BfuI GTATCC 1 cut(s) 59
BisI GCNGC 1 cut(s) 127
BlpI GCTNAGC 1 cut(s) 275
BlsI GCNGC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 222, 288
Bpu1102I GCTNAGC 1 cut(s) 275
Bpu14I TTCGAA 2 cut(s) 257, 648
Bsa29I ATCGAT 1 cut(s) 618
BsaJI CCNNGG 2 cut(s) 160, 432
BsaWI WCCGGW 1 cut(s) 62
BsaXI ACNNNNNCTCC 2 cut(s) 513, 543
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse118I RCCGGY 2 cut(s) 62, 103
Bse1I ACTGG 1 cut(s) 558
BseCI ATCGAT 1 cut(s) 618
BseDI CCNNGG 2 cut(s) 160, 432
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 1 cut(s) 558
BseRI GAGGAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 62
BseX3I CGGCCG 1 cut(s) 105
BseXI GCAGC 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 6 cut(s) 100, 107, 165, 172, 224, 289
BshTI ACCGGT 1 cut(s) 62
BshVI ATCGAT 1 cut(s) 618
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 62, 281
BsiSI CCGG 2 cut(s) 63, 104
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 1 cut(s) 16
BsmAI GTCTC 2 cut(s) 454, 570
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 1 cut(s) 656
BsnI GGCC 6 cut(s) 100, 107, 165, 172, 224, 289
Bsp119I TTCGAA 2 cut(s) 257, 648
Bsp1286I GDGCHC 2 cut(s) 62, 281
Bsp1407I TGTACA 1 cut(s) 88
Bsp143I GATC 5 cut(s) 6, 73, 134, 156, 615
Bsp1720I GCTNAGC 1 cut(s) 275
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 1 cut(s) 391
BspANI GGCC 6 cut(s) 100, 107, 165, 172, 224, 289
BspCNI CTCAG 1 cut(s) 267
BspDI ATCGAT 1 cut(s) 618
BspPI GGATC 1 cut(s) 151
BspT104I TTCGAA 2 cut(s) 257, 648
BsrBI CCGCTC 1 cut(s) 391
BsrFI RCCGGY 2 cut(s) 62, 103
BsrGI TGTACA 1 cut(s) 88
BsrI ACTGG 1 cut(s) 558
BssAI RCCGGY 2 cut(s) 62, 103
BssECI CCNNGG 2 cut(s) 160, 432
BssMI GATC 5 cut(s) 6, 73, 134, 156, 615
BssT1I CCWWGG 2 cut(s) 160, 432
Bst4CI ACNGT 1 cut(s) 613
BstAUI TGTACA 1 cut(s) 88
BstBI TTCGAA 2 cut(s) 257, 648
BstC8I GCNNGC 1 cut(s) 272
BstDEI CTNAG 1 cut(s) 275
BstDSI CCRYGG 1 cut(s) 160
BstKTI GATC 5 cut(s) 9, 76, 137, 159, 618
BstMAI GTCTC 2 cut(s) 454, 570
BstMBI GATC 5 cut(s) 6, 73, 134, 156, 615
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstNSI RCATGY 2 cut(s) 354, 406
BstSLI GKGCMC 1 cut(s) 62
BstV1I GCAGC 1 cut(s) 113
BstZI CGGCCG 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 618
BsuI GTATCC 1 cut(s) 59
BsuRI GGCC 6 cut(s) 100, 107, 165, 172, 224, 289
BsuTUI ATCGAT 1 cut(s) 618
BtgI CCRYGG 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 272
Cfr10I RCCGGY 2 cut(s) 62, 103
Cfr13I GGNCC 2 cut(s) 222, 288
ClaI ATCGAT 1 cut(s) 618
CseI GACGC 1 cut(s) 613
Csp6I GTAC 1 cut(s) 89
CspAI ACCGGT 1 cut(s) 62
CviAII CATG 5 cut(s) 55, 161, 344, 351, 403
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 1 cut(s) 275
DpnI GATC 5 cut(s) 8, 75, 136, 158, 617
DpnII GATC 5 cut(s) 6, 73, 134, 156, 615
DraI TTTAAA 1 cut(s) 82
EaeI YGGCCR 3 cut(s) 98, 105, 170
EagI CGGCCG 1 cut(s) 105
EclXI CGGCCG 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 160, 432
Eco52I CGGCCG 1 cut(s) 105
EcoT14I CCWWGG 2 cut(s) 160, 432
ErhI CCWWGG 2 cut(s) 160, 432
FaeI CATG 5 cut(s) 58, 164, 347, 354, 406
FalI AAGNNNNNCTT 2 cut(s) 554, 586
FaqI GGGAC 1 cut(s) 350
FatI CATG 5 cut(s) 54, 160, 343, 350, 402
FauNDI CATATG 1 cut(s) 70
FbaI TGATCA 1 cut(s) 73
FblI GTMKAC 1 cut(s) 631
Fnu4HI GCNGC 1 cut(s) 127
Fsp4HI GCNGC 1 cut(s) 127
FspBI CTAG 1 cut(s) 252
GluI GCNGC 1 cut(s) 127
HaeIII GGCC 6 cut(s) 100, 107, 165, 172, 224, 289
HapII CCGG 2 cut(s) 63, 104
HgaI GACGC 1 cut(s) 613
Hin1II CATG 5 cut(s) 58, 164, 347, 354, 406
HincII GTYRAC 1 cut(s) 632
HindII GTYRAC 1 cut(s) 632
HpaII CCGG 2 cut(s) 63, 104
Hpy166II GTNNAC 3 cut(s) 60, 539, 632
Hpy188III TCNNGA 3 cut(s) 10, 154, 571
Hpy8I GTNNAC 3 cut(s) 60, 539, 632
HpyAV CCTTC 1 cut(s) 655
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 3 cut(s) 119, 185, 471
HpyCH4V TGCA 7 cut(s) 29, 60, 129, 305, 361, 509, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 1 cut(s) 275
HpySE526I ACGT 3 cut(s) 119, 185, 471
Hsp92II CATG 5 cut(s) 58, 164, 347, 354, 406
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 5 cut(s) 6, 73, 134, 156, 615
LpnPI CCDG 6 cut(s) 23, 76, 108, 117, 434, 571
Lsp1109I GCAGC 1 cut(s) 113
MaeI CTAG 1 cut(s) 252
MaeII ACGT 3 cut(s) 119, 185, 471
MaeIII GTNAC 2 cut(s) 472, 502
MalI GATC 5 cut(s) 8, 75, 136, 158, 617
MbiI CCGCTC 1 cut(s) 391
MboI GATC 5 cut(s) 6, 73, 134, 156, 615
MhlI GDGCHC 2 cut(s) 62, 281
MlsI TGGCCA 2 cut(s) 100, 172
MluCI AATT 3 cut(s) 83, 231, 259
MluNI TGGCCA 2 cut(s) 100, 172
MnlI CCTC 4 cut(s) 292, 452, 536, 539
Mox20I TGGCCA 2 cut(s) 100, 172
MscI TGGCCA 2 cut(s) 100, 172
MseI TTAA 2 cut(s) 81, 234
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 2 cut(s) 100, 172
MspI CCGG 2 cut(s) 63, 104
Mva1269I GAATGC 1 cut(s) 656
MwoI GCNNNNNNNGC 1 cut(s) 276
NcoI CCATGG 1 cut(s) 160
NdeI CATATG 1 cut(s) 70
NdeII GATC 5 cut(s) 6, 73, 134, 156, 615
NlaIII CATG 5 cut(s) 58, 164, 347, 354, 406
NmuCI GTSAC 1 cut(s) 472
NspI RCATGY 2 cut(s) 354, 406
NspV TTCGAA 2 cut(s) 257, 648
PciI ACATGT 2 cut(s) 350, 402
PctI GAATGC 1 cut(s) 656
PinAI ACCGGT 1 cut(s) 62
PkrI GCNGC 1 cut(s) 128
PscI ACATGT 2 cut(s) 350, 402
PspPI GGNCC 2 cut(s) 222, 288
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 1 cut(s) 552
SalI GTCGAC 1 cut(s) 630
SaqAI TTAA 2 cut(s) 81, 234
SatI GCNGC 1 cut(s) 127
Sau3AI GATC 5 cut(s) 6, 73, 134, 156, 615
Sau96I GGNCC 2 cut(s) 222, 288
SduI GDGCHC 2 cut(s) 62, 281
SetI ASST 8 cut(s) 122, 188, 276, 284, 423, 434, 474, 647
SfuI TTCGAA 2 cut(s) 257, 648
SmiMI CAYNNNNRTG 1 cut(s) 552
SpeI ACTAGT 1 cut(s) 251
Sse9I AATT 3 cut(s) 83, 231, 259
SsiI CCGC 1 cut(s) 391
SspMI CTAG 1 cut(s) 252
StyI CCWWGG 2 cut(s) 160, 432
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 3 cut(s) 122, 188, 474
TaqI TCGA 5 cut(s) 257, 387, 618, 631, 648
TasI AATT 3 cut(s) 83, 231, 259
TatI WGTACW 1 cut(s) 88
Tru1I TTAA 2 cut(s) 81, 234
Tru9I TTAA 2 cut(s) 81, 234
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 1 cut(s) 126
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 1 cut(s) 57
TspGWI ACGGA 2 cut(s) 521, 675
VneI GTGCAC 1 cut(s) 58
XapI RAATTY 2 cut(s) 83, 259
XceI RCATGY 2 cut(s) 354, 406
XmiI GTMKAC 1 cut(s) 631
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.