RchiOBHm_Chr3g0457381

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000345
Physical Location & Seq
Forward (+)
6533526 .. 6534335
810 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42413

Sequence Viewer

Length: 681 bp
ATGGCATTCCTAGCAGTTAGGACACTAATTGTTCTTATGCTTATTTCTACATTTGCTAAAGCCGCAAACAAATATGACTATCTACAACTAGTGCAACAATGGCCTAAAACGTTCTGCCATAATAACCGAGCTTGCATTCAAGGTGCAGTCCTCCCGGAGCTGTTCTCGATACATGGTATGTGGCCATCTAATTTCTCCGGCCAGAACGACGCTTGTGTTGGAACTCGGTTCAGCATGAGAGAGATGCGTCGACACAATCATCTCGAAACTCAATTGCTGTCATCCTCATGGCCAAGCTACACTGGTCTGTCCAACCTGCACTTTTGGGAGTATGAGTATAACAAGCACGGCACTTGTTCAGAGAATAACCTCAAACAGACGGACTATTTCACCAGAGCCGACGCTTTGTGGAGGCGCTACAATGTTACCAATATGCTTTTAACATCGCGCCACCAAATCTCGCCGGGATCCTCCTATCGTTACGCTGACATTTTGTATGCCATTCGACAAGGAATTGGGGGATACACTCCTTTGATTCTTTGCAAGCAAGATCCGGCAAATAATATTTGGATTCTGCACGAGGTGATAATTTGTTTCAATCCCTTGGGGAATAACGTCATCGCTTGTCGCAGAAGATCGAGATATAGTTGCAACAGCGGGTTGATAGACTATCCTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

226

Amino Acids

26.16

Weight (kDa)

9.23

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 25 - 209 4.4e-36 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 250
AccII CGCG 1 cut(s) 448
AciI CCGC 2 cut(s) 63, 657
AclI AACGTT 1 cut(s) 110
AclWI GGATC 3 cut(s) 462, 475, 545
AcoI YGGCCR 3 cut(s) 182, 199, 290
AdeI CACNNNGTG 1 cut(s) 583
AgsI TTSAA 2 cut(s) 140, 598
AhlI ACTAGT 1 cut(s) 88
AluBI AGCT 3 cut(s) 131, 160, 297
AluI AGCT 3 cut(s) 131, 160, 297
AlwI GGATC 3 cut(s) 462, 475, 545
AoxI GGCC 4 cut(s) 101, 182, 199, 290
AspLEI GCGC 2 cut(s) 417, 450
AsuC2I CCSGG 2 cut(s) 155, 465
AsuHPI GGTGA 2 cut(s) 382, 595
BalI TGGCCA 2 cut(s) 184, 292
BamHI GGATCC 1 cut(s) 467
BauI CACGAG 1 cut(s) 578
BccI CCATC 1 cut(s) 193
BceAI ACGGC 1 cut(s) 364
BciVI GTATCC 1 cut(s) 515
BcnI CCSGG 2 cut(s) 155, 465
BcuI ACTAGT 1 cut(s) 88
BfaI CTAG 2 cut(s) 11, 89
BfoI RGCGCY 1 cut(s) 418
BfuAI ACCTGC 1 cut(s) 324
BfuI GTATCC 1 cut(s) 515
BisI GCNGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 64
Bme1390I CCNGG 2 cut(s) 155, 465
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 2 cut(s) 155, 465
BmsI GCATC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpuMI CCSGG 2 cut(s) 155, 465
BsaJI CCNNGG 1 cut(s) 603
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bse1I ACTGG 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 603
BseGI GGATG 1 cut(s) 281
BseNI ACTGG 1 cut(s) 307
BsgI GTGCAG 3 cut(s) 165, 302, 560
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 4 cut(s) 103, 184, 201, 292
BsiSI CCGG 4 cut(s) 155, 198, 464, 554
BsmI GAATGC 2 cut(s) 5, 135
BsnI GGCC 4 cut(s) 103, 184, 201, 292
Bsp143I GATC 3 cut(s) 467, 550, 635
BspACI CCGC 2 cut(s) 63, 657
BspANI GGCC 4 cut(s) 103, 184, 201, 292
BspFNI CGCG 1 cut(s) 448
BspLI GGNNCC 1 cut(s) 469
BspMI ACCTGC 1 cut(s) 324
BspPI GGATC 3 cut(s) 462, 475, 545
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 603
BssMI GATC 3 cut(s) 467, 550, 635
BssSI CACGAG 1 cut(s) 578
BssT1I CCWWGG 1 cut(s) 603
Bst2BI CACGAG 1 cut(s) 578
BstC8I GCNNGC 2 cut(s) 133, 545
BstDEI CTNAG 1 cut(s) 675
BstF5I GGATG 1 cut(s) 281
BstFNI CGCG 1 cut(s) 448
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 2 cut(s) 417, 450
BstKTI GATC 3 cut(s) 470, 553, 638
BstMBI GATC 3 cut(s) 467, 550, 635
BstMWI GCNNNNNNNGC 3 cut(s) 11, 62, 100
BstSCI CCNGG 2 cut(s) 153, 463
BstUI CGCG 1 cut(s) 448
BstX2I RGATCY 2 cut(s) 467, 550
BstYI RGATCY 2 cut(s) 467, 550
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 4 cut(s) 103, 184, 201, 292
BtgZI GCGATG 2 cut(s) 429, 604
BtsCI GGATG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 133, 545
CfoI GCGC 2 cut(s) 417, 450
CseI GACGC 3 cut(s) 218, 236, 410
CviAII CATG 3 cut(s) 173, 235, 288
CviJI RGCY 9 cut(s) 62, 103, 131, 160, 184, 201, 292, 297, 398
CviKI_1 RGCY 9 cut(s) 62, 103, 131, 160, 184, 201, 292, 297, 398
DdeI CTNAG 1 cut(s) 675
DpnI GATC 3 cut(s) 469, 552, 637
DpnII GATC 3 cut(s) 467, 550, 635
DraIII CACNNNGTG 1 cut(s) 583
EaeI YGGCCR 3 cut(s) 182, 199, 290
Eco130I CCWWGG 1 cut(s) 603
EcoT14I CCWWGG 1 cut(s) 603
ErhI CCWWGG 1 cut(s) 603
FaeI CATG 3 cut(s) 176, 238, 291
FatI CATG 3 cut(s) 172, 234, 287
FauI CCCGC 1 cut(s) 650
FblI GTMKAC 1 cut(s) 250
Fnu4HI GCNGC 1 cut(s) 63
FokI GGATG 1 cut(s) 268
Fsp4HI GCNGC 1 cut(s) 63
FspBI CTAG 2 cut(s) 11, 89
GlaI GCGC 2 cut(s) 416, 449
GluI GCNGC 1 cut(s) 63
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 4 cut(s) 103, 184, 201, 292
HapII CCGG 4 cut(s) 155, 198, 464, 554
HgaI GACGC 3 cut(s) 218, 236, 410
HhaI GCGC 2 cut(s) 417, 450
Hin1II CATG 3 cut(s) 176, 238, 291
Hin6I GCGC 2 cut(s) 415, 448
HinP1I GCGC 2 cut(s) 415, 448
HincII GTYRAC 1 cut(s) 251
HindII GTYRAC 1 cut(s) 251
HinfI GANTC 2 cut(s) 535, 571
HpaII CCGG 4 cut(s) 155, 198, 464, 554
HphI GGTGA 2 cut(s) 382, 595
Hpy166II GTNNAC 1 cut(s) 251
Hpy188I TCNGA 1 cut(s) 361
Hpy188III TCNNGA 3 cut(s) 166, 263, 639
Hpy8I GTNNAC 1 cut(s) 251
Hpy99I CGWCG 3 cut(s) 212, 252, 404
HpyCH4IV ACGT 2 cut(s) 110, 615
HpyCH4V TGCA 7 cut(s) 94, 135, 146, 319, 543, 577, 651
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 62, 100
HpyF3I CTNAG 1 cut(s) 675
HpySE526I ACGT 2 cut(s) 110, 615
Hsp92II CATG 3 cut(s) 176, 238, 291
HspAI GCGC 2 cut(s) 415, 448
Kzo9I GATC 3 cut(s) 467, 550, 635
LmnI GCTCC 1 cut(s) 157
LpnPI CCDG 8 cut(s) 168, 211, 215, 288, 329, 406, 477, 567
LweI GCATC 1 cut(s) 234
MaeI CTAG 2 cut(s) 11, 89
MaeII ACGT 2 cut(s) 110, 615
MaeIII GTNAC 2 cut(s) 424, 479
MalI GATC 3 cut(s) 469, 552, 637
MboI GATC 3 cut(s) 467, 550, 635
MboII GAAGA 1 cut(s) 645
MfeI CAATTG 1 cut(s) 272
MflI RGATCY 2 cut(s) 467, 550
MlsI TGGCCA 2 cut(s) 184, 292
MluCI AATT 5 cut(s) 27, 190, 272, 513, 588
MluNI TGGCCA 2 cut(s) 184, 292
MmeI TCCRAC 2 cut(s) 199, 336
MnlI CCTC 6 cut(s) 161, 295, 380, 405, 481, 574
Mox20I TGGCCA 2 cut(s) 184, 292
MscI TGGCCA 2 cut(s) 184, 292
MseI TTAA 1 cut(s) 440
MslI CAYNNNNRTG 1 cut(s) 286
Msp20I TGGCCA 2 cut(s) 184, 292
MspA1I CMGCKG 1 cut(s) 657
MspI CCGG 4 cut(s) 155, 198, 464, 554
MspR9I CCNGG 2 cut(s) 155, 465
MunI CAATTG 1 cut(s) 272
Mva1269I GAATGC 2 cut(s) 5, 135
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 3 cut(s) 11, 62, 100
NciI CCSGG 2 cut(s) 155, 465
NdeII GATC 3 cut(s) 467, 550, 635
NlaIII CATG 3 cut(s) 176, 238, 291
NlaIV GGNNCC 1 cut(s) 469
PctI GAATGC 2 cut(s) 5, 135
PfeI GAWTC 2 cut(s) 535, 571
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 1 cut(s) 64
Psp1406I AACGTT 1 cut(s) 110
PspN4I GGNNCC 1 cut(s) 469
PsuI RGATCY 2 cut(s) 467, 550
RseI CAYNNNNRTG 1 cut(s) 286
SalI GTCGAC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 1 cut(s) 63
Sau3AI GATC 3 cut(s) 467, 550, 635
ScrFI CCNGG 2 cut(s) 155, 465
SetI ASST 9 cut(s) 113, 133, 145, 162, 299, 318, 372, 585, 618
SfaNI GCATC 1 cut(s) 234
SmiMI CAYNNNNRTG 1 cut(s) 286
SpeI ACTAGT 1 cut(s) 88
Sse9I AATT 5 cut(s) 27, 190, 272, 513, 588
SsiI CCGC 2 cut(s) 63, 657
SspI AATATT 1 cut(s) 565
SspMI CTAG 2 cut(s) 11, 89
StyD4I CCNGG 2 cut(s) 153, 463
StyI CCWWGG 1 cut(s) 603
TaiI ACGT 2 cut(s) 113, 618
TaqI TCGA 5 cut(s) 167, 250, 264, 505, 638
TasI AATT 5 cut(s) 27, 190, 272, 513, 588
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 2 cut(s) 535, 571
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 1 cut(s) 307
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 1 cut(s) 307
XmiI GTMKAC 1 cut(s) 250
XspI CTAG 2 cut(s) 11, 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.