RchiOBHm_Chr4g0398371

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000345
Physical Location & Seq
Forward (+)
14898592 .. 14899257
666 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37055

Sequence Viewer

Length: 570 bp
ATGAGAACACCAGCAGTCTGTCTTATTTTCTTGCTCTCTGTTGGTCTGTTCAAGGGTTCCCATGCAGAGCCATTCGAATACCTACAGTTTGTCTTGCAATACGCTAGAGGTTCCTGTGTTAACGTCAAGAAATGCATTCCACCGGCGAGATTGCCGGCCAAATTCACGGTTCATGGAATTTGGCCCACGAATATTTCCAAGCAGGAGGTGATCGAGTGTACAGCTGCCGTGAAACTACACTCGTTCGACGGAAACCTGATAACACCGTCGCTGAAAACCGATCTTCTCCAATCTTGGCCCAGCGTCCGGAGAGATAAAGATAATATGACTTTTTGGGAACACGAATATAATAAGCATGGGTCCTGTACTGCACCAGCTATCACTCAGACAGCGTATTTTGAGAGAGCTCACAAGTTCTGGAAGGAGTATGATCTCTATTCTGTTTTGGAACAAAAAAAATCAAGCCGGGGCAGTCTCGATGGTATTCACTGGCTGATTTTCAAGCAGCCGTCCAGTCCAAGATTAGTACCGATTATATACCTCTCATCCTGTGCAAGGAGGAAGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

189

Amino Acids

21.68

Weight (kDa)

9.42

Isoelectric Point (pI)

60.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 25 - 152 8.8e-27 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 306
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 161, 177
AfaI GTAC 3 cut(s) 220, 367, 528
AfiI CCNNNNNNNGG 2 cut(s) 306, 555
AgsI TTSAA 2 cut(s) 52, 502
AluBI AGCT 3 cut(s) 224, 377, 407
AluI AGCT 3 cut(s) 224, 377, 407
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 479
Aor13HI TCCGGA 1 cut(s) 306
AoxI GGCC 3 cut(s) 156, 182, 296
ApeKI GCWGC 2 cut(s) 224, 505
ApoI RAATTY 2 cut(s) 161, 177
AspS9I GGNCC 3 cut(s) 183, 297, 360
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 220
AsuII TTCGAA 1 cut(s) 75
AvaII GGWCC 1 cut(s) 360
BanII GRGCYC 1 cut(s) 409
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 2 cut(s) 211, 517
BccI CCATC 1 cut(s) 473
BceAI ACGGC 2 cut(s) 212, 493
BcnI CCSGG 1 cut(s) 467
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 83
BisI GCNGC 2 cut(s) 225, 506
BlsI GCNGC 2 cut(s) 226, 507
Bme1390I CCNGG 1 cut(s) 467
Bme18I GGWCC 1 cut(s) 360
BmgT120I GGNCC 3 cut(s) 183, 297, 360
BmiI GGNNCC 3 cut(s) 58, 112, 361
BmrFI CCNGG 1 cut(s) 467
Bpu14I TTCGAA 1 cut(s) 75
BpuMI CCSGG 1 cut(s) 467
BsaJI CCNNGG 1 cut(s) 466
BsaWI WCCGGW 1 cut(s) 306
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 555
Bse118I RCCGGY 2 cut(s) 142, 154
Bse1I ACTGG 2 cut(s) 494, 513
BseAI TCCGGA 1 cut(s) 306
BseDI CCNNGG 1 cut(s) 466
BseGI GGATG 1 cut(s) 545
BseLI CCNNNNNNNGG 2 cut(s) 306, 555
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 2 cut(s) 494, 513
BseXI GCAGC 2 cut(s) 211, 517
BseYI CCCAGC 1 cut(s) 299
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 3 cut(s) 158, 184, 298
BsiHKAI GWGCWC 1 cut(s) 409
BsiSI CCGG 4 cut(s) 143, 155, 307, 466
BslI CCNNNNNNNGG 2 cut(s) 306, 555
BsmAI GTCTC 1 cut(s) 479
BsmI GAATGC 1 cut(s) 135
BsnI GGCC 3 cut(s) 158, 184, 298
Bsp119I TTCGAA 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 409
Bsp13I TCCGGA 1 cut(s) 306
Bsp1407I TGTACA 1 cut(s) 218
Bsp143I GATC 3 cut(s) 210, 280, 430
BspANI GGCC 3 cut(s) 158, 184, 298
BspCNI CTCAG 1 cut(s) 397
BspEI TCCGGA 1 cut(s) 306
BspLI GGNNCC 3 cut(s) 58, 112, 361
BspT104I TTCGAA 1 cut(s) 75
BsrFI RCCGGY 2 cut(s) 142, 154
BsrGI TGTACA 1 cut(s) 218
BsrI ACTGG 2 cut(s) 494, 513
BssAI RCCGGY 2 cut(s) 142, 154
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 3 cut(s) 210, 280, 430
Bst4CI ACNGT 3 cut(s) 87, 169, 267
BstAUI TGTACA 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 384
BstENI CCTNNNNNAGG 1 cut(s) 553
BstF5I GGATG 1 cut(s) 545
BstKTI GATC 3 cut(s) 213, 283, 433
BstMAI GTCTC 1 cut(s) 479
BstMBI GATC 3 cut(s) 210, 280, 430
BstSCI CCNGG 1 cut(s) 465
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 211, 517
BsuRI GGCC 3 cut(s) 158, 184, 298
BtsCI GGATG 1 cut(s) 545
BtsIMutI CAGTG 1 cut(s) 487
Cac8I GCNNGC 1 cut(s) 156
Cfr10I RCCGGY 2 cut(s) 142, 154
Cfr13I GGNCC 3 cut(s) 183, 297, 360
CseI GACGC 1 cut(s) 292
Csp6I GTAC 3 cut(s) 219, 366, 527
CviAII CATG 3 cut(s) 62, 173, 356
CviQI GTAC 3 cut(s) 219, 366, 527
DdeI CTNAG 1 cut(s) 384
DpnI GATC 3 cut(s) 212, 282, 432
DpnII GATC 3 cut(s) 210, 280, 430
EaeI YGGCCR 1 cut(s) 156
Ecl136II GAGCTC 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 409
Eco47I GGWCC 1 cut(s) 360
Eco53kI GAGCTC 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 553
EcoO109I RGGNCCY 1 cut(s) 360
EcoT22I ATGCAT 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 3 cut(s) 65, 176, 359
FaiI YATR 8 cut(s) 63, 174, 326, 348, 357, 429, 536, 538
FatI CATG 3 cut(s) 61, 172, 355
Fnu4HI GCNGC 2 cut(s) 225, 506
FokI GGATG 1 cut(s) 532
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 2 cut(s) 225, 506
FspBI CTAG 1 cut(s) 105
GluI GCNGC 2 cut(s) 225, 506
GsaI CCCAGC 1 cut(s) 303
HaeIII GGCC 3 cut(s) 158, 184, 298
HapII CCGG 4 cut(s) 143, 155, 307, 466
HgaI GACGC 1 cut(s) 292
Hin1II CATG 3 cut(s) 65, 176, 359
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HpaI GTTAAC 1 cut(s) 121
HpaII CCGG 4 cut(s) 143, 155, 307, 466
HphI GGTGA 1 cut(s) 220
Hpy166II GTNNAC 2 cut(s) 121, 219
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 4 cut(s) 127, 307, 418, 476
Hpy8I GTNNAC 2 cut(s) 121, 219
Hpy99I CGWCG 2 cut(s) 251, 271
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 3 cut(s) 87, 169, 267
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 65, 97, 135, 371, 554
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 3 cut(s) 65, 176, 359
Kpn2I TCCGGA 1 cut(s) 306
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 3 cut(s) 210, 280, 430
Lsp1109I GCAGC 2 cut(s) 211, 517
MaeI CTAG 1 cut(s) 105
MaeII ACGT 1 cut(s) 123
MalI GATC 3 cut(s) 212, 282, 432
MboI GATC 3 cut(s) 210, 280, 430
MboII GAAGA 1 cut(s) 275
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 2 cut(s) 161, 177
MnlI CCTC 4 cut(s) 101, 199, 551, 552
Mph1103I ATGCAT 1 cut(s) 137
MroI TCCGGA 1 cut(s) 306
MroNI GCCGGC 1 cut(s) 154
MseI TTAA 2 cut(s) 120, 568
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 4 cut(s) 143, 155, 307, 466
MspR9I CCNGG 1 cut(s) 467
Mva1269I GAATGC 1 cut(s) 135
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 467
NdeII GATC 3 cut(s) 210, 280, 430
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 3 cut(s) 65, 176, 359
NlaIV GGNNCC 3 cut(s) 58, 112, 361
NsiI ATGCAT 1 cut(s) 137
NspV TTCGAA 1 cut(s) 75
PctI GAATGC 1 cut(s) 135
PdiI GCCGGC 1 cut(s) 156
PkrI GCNGC 2 cut(s) 226, 507
PpuMI RGGWCCY 1 cut(s) 360
Psp124BI GAGCTC 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 360
PspFI CCCAGC 1 cut(s) 299
PspN4I GGNNCC 3 cut(s) 58, 112, 361
PspPI GGNCC 3 cut(s) 183, 297, 360
PspPPI RGGWCCY 1 cut(s) 360
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 3 cut(s) 220, 367, 528
RsaNI GTAC 3 cut(s) 219, 366, 527
SacI GAGCTC 1 cut(s) 409
SaqAI TTAA 2 cut(s) 120, 568
SatI GCNGC 2 cut(s) 225, 506
Sau3AI GATC 3 cut(s) 210, 280, 430
Sau96I GGNCC 3 cut(s) 183, 297, 360
ScrFI CCNGG 1 cut(s) 467
SduI GDGCHC 1 cut(s) 409
SetI ASST 9 cut(s) 84, 112, 126, 210, 226, 258, 379, 409, 543
SfcI CTRYAG 1 cut(s) 83
SfuI TTCGAA 1 cut(s) 75
SgrAI CRCCGGYG 1 cut(s) 142
SinI GGWCC 1 cut(s) 360
Sse9I AATT 2 cut(s) 161, 177
SspI AATATT 1 cut(s) 193
SspMI CTAG 1 cut(s) 105
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 1 cut(s) 465
TaaI ACNGT 3 cut(s) 87, 169, 267
TaiI ACGT 1 cut(s) 126
TaqI TCGA 4 cut(s) 75, 213, 246, 477
TasI AATT 2 cut(s) 161, 177
TatI WGTACW 2 cut(s) 218, 365
Tru1I TTAA 2 cut(s) 120, 568
Tru9I TTAA 2 cut(s) 120, 568
TscAI CASTG 1 cut(s) 494
TseI GCWGC 2 cut(s) 224, 505
TspDTI ATGAA 1 cut(s) 161
TspGWI ACGGA 1 cut(s) 264
TspRI CASTG 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 360
XagI CCTNNNNNAGG 1 cut(s) 553
XapI RAATTY 2 cut(s) 161, 177
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.