Rroxscaffold_6G00418220

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000345
Physical Location & Seq
Reverse (-)
39780719 .. 39781409
691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00418220.1

Sequence Viewer

Length: 504 bp
ATGGCCGGCTACATTCTGCTATCAAAATCCGGGTTGTGTCCGGAATCCCCCGCGGAATACTCTTACCATTCATGGCCTTTGGCCAAACTATTGTTTCTGCCGCAAGCATGGCCCCAGTTAATTGCTCCTTTCACCGACATGCAATTCTGGCAGACCGAATATGACAAGCACGGTACCTGCTCGGAGAACAAACTCAAGCAGACGGAGTATTTTAGGACAGCTCACTGGGCGTGGGTTCGATTCAATGCATATTTTCTGTTCGCACACTCGCCTCTCCAAATCCACCCTGGTAATTACTATCATAAAGTTGACCTTGAACAAGCCATTCGGGCAGTCACTACTAAGGACCCTCTGCTTATGTGCAAGAAGATCAAACCCATGGGCATTGATTCATGGCTATTGCAGGAAGTCGTCATCTGTCTTGATGTTTTGGGAGTCCAACCGGTCTCTTGTCGCCCTCGATTGTCGAGTTGCGGCGGTCCAATTATATATTATGGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

167

Amino Acids

19.26

Weight (kDa)

8.18

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 32 - 151 1.8e-17 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
Acc65I GGTACC 1 cut(s) 173
AccB1I GGYRCC 1 cut(s) 173
AccII CGCG 1 cut(s) 53
AccIII TCCGGA 1 cut(s) 40
AciI CCGC 5 cut(s) 51, 53, 101, 474, 477
AcoI YGGCCR 2 cut(s) 3, 81
AfaI GTAC 1 cut(s) 175
AgeI ACCGGT 1 cut(s) 442
AgsI TTSAA 2 cut(s) 244, 317
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 1 cut(s) 221
AluI AGCT 1 cut(s) 221
Alw26I GTCTC 1 cut(s) 451
Aor13HI TCCGGA 1 cut(s) 40
AoxI GGCC 4 cut(s) 3, 74, 81, 110
AsiGI ACCGGT 1 cut(s) 442
Asp718I GGTACC 1 cut(s) 173
AspS9I GGNCC 3 cut(s) 111, 346, 479
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 124
AvaII GGWCC 2 cut(s) 346, 479
BalI TGGCCA 1 cut(s) 83
BanI GGYRCC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 288
BcnI CCSGG 1 cut(s) 31
BcoDI GTCTC 1 cut(s) 451
BfuAI ACCTGC 1 cut(s) 185
BglI GCCNNNNNGGC 1 cut(s) 329
BisI GCNGC 2 cut(s) 101, 475
BlsI GCNGC 2 cut(s) 102, 476
Bme1390I CCNGG 2 cut(s) 31, 288
Bme18I GGWCC 2 cut(s) 346, 479
BmgT120I GGNCC 3 cut(s) 111, 346, 479
BmiI GGNNCC 3 cut(s) 113, 175, 348
BmrFI CCNGG 2 cut(s) 31, 288
BmrI ACTGGG 2 cut(s) 109, 235
BmuI ACTGGG 2 cut(s) 109, 235
BpuEI CTTGAG 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 31
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 3 cut(s) 51, 286, 378
BsaWI WCCGGW 2 cut(s) 40, 442
Bse118I RCCGGY 2 cut(s) 5, 442
Bse1I ACTGG 2 cut(s) 115, 230
BseAI TCCGGA 1 cut(s) 40
BseBI CCWGG 1 cut(s) 288
BseDI CCNNGG 3 cut(s) 51, 286, 378
BseNI ACTGG 2 cut(s) 115, 230
Bsh1236I CGCG 1 cut(s) 53
BshFI GGCC 4 cut(s) 5, 76, 83, 112
BshNI GGYRCC 1 cut(s) 173
BshTI ACCGGT 1 cut(s) 442
BsiSI CCGG 4 cut(s) 6, 30, 41, 443
BsmAI GTCTC 1 cut(s) 451
BsnI GGCC 4 cut(s) 5, 76, 83, 112
Bso31I GGTCTC 1 cut(s) 451
Bsp13I TCCGGA 1 cut(s) 40
Bsp143I GATC 1 cut(s) 369
Bsp19I CCATGG 1 cut(s) 378
BspACI CCGC 5 cut(s) 51, 53, 101, 474, 477
BspANI GGCC 4 cut(s) 5, 76, 83, 112
BspEI TCCGGA 1 cut(s) 40
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 3 cut(s) 113, 175, 348
BspMI ACCTGC 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 451
BsrFI RCCGGY 2 cut(s) 5, 442
BsrI ACTGG 2 cut(s) 115, 230
BssAI RCCGGY 2 cut(s) 5, 442
BssECI CCNNGG 3 cut(s) 51, 286, 378
BssMI GATC 1 cut(s) 369
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 7, 105
BstDEI CTNAG 1 cut(s) 342
BstDSI CCRYGG 2 cut(s) 51, 378
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 1 cut(s) 372
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 1 cut(s) 369
BstMWI GCNNNNNNNGC 4 cut(s) 109, 148, 227, 329
BstNI CCWGG 1 cut(s) 288
BstNSI RCATGY 1 cut(s) 142
BstSCI CCNGG 2 cut(s) 29, 286
BstUI CGCG 1 cut(s) 53
BsuRI GGCC 4 cut(s) 5, 76, 83, 112
BtgI CCRYGG 2 cut(s) 51, 378
BtsIMutI CAGTG 1 cut(s) 223
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 2 cut(s) 7, 105
Cfr10I RCCGGY 2 cut(s) 5, 442
Cfr13I GGNCC 3 cut(s) 111, 346, 479
Cfr42I CCGCGG 1 cut(s) 54
Csp6I GTAC 1 cut(s) 174
CspAI ACCGGT 1 cut(s) 442
CviAII CATG 5 cut(s) 72, 108, 139, 379, 393
CviJI RGCY 8 cut(s) 5, 9, 76, 83, 112, 221, 323, 397
CviKI_1 RGCY 8 cut(s) 5, 9, 76, 83, 112, 221, 323, 397
CviQI GTAC 1 cut(s) 174
DdeI CTNAG 1 cut(s) 342
DpnI GATC 1 cut(s) 371
DpnII GATC 1 cut(s) 369
EaeI YGGCCR 2 cut(s) 3, 81
Eco130I CCWWGG 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 451
Eco47I GGWCC 2 cut(s) 346, 479
EcoO109I RGGNCCY 1 cut(s) 346
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 378
EcoT22I ATGCAT 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 5 cut(s) 75, 111, 142, 382, 396
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FatI CATG 5 cut(s) 71, 107, 138, 378, 392
FauI CCCGC 1 cut(s) 58
Fnu4HI GCNGC 2 cut(s) 101, 475
Fsp4HI GCNGC 2 cut(s) 101, 475
GluI GCNGC 2 cut(s) 101, 475
HaeIII GGCC 4 cut(s) 5, 76, 83, 112
HapII CCGG 4 cut(s) 6, 30, 41, 443
Hin1II CATG 5 cut(s) 75, 111, 142, 382, 396
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 4 cut(s) 44, 240, 389, 435
HpaII CCGG 4 cut(s) 6, 30, 41, 443
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 1 cut(s) 184
Hpy188III TCNNGA 2 cut(s) 41, 422
Hpy8I GTNNAC 1 cut(s) 310
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4V TGCA 4 cut(s) 142, 248, 363, 403
HpyF10VI GCNNNNNNNGC 4 cut(s) 109, 148, 227, 329
HpyF3I CTNAG 1 cut(s) 342
Hsp92II CATG 5 cut(s) 75, 111, 142, 382, 396
Kpn2I TCCGGA 1 cut(s) 40
KpnI GGTACC 1 cut(s) 177
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
KspI CCGCGG 1 cut(s) 54
Kzo9I GATC 1 cut(s) 369
LmnI GCTCC 1 cut(s) 130
MaeIII GTNAC 2 cut(s) 334, 497
MalI GATC 1 cut(s) 371
MboI GATC 1 cut(s) 369
MboII GAAGA 1 cut(s) 379
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 4 cut(s) 120, 143, 292, 483
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 1 cut(s) 444
MmeI TCCRAC 1 cut(s) 463
MnlI CCTC 3 cut(s) 282, 360, 468
Mox20I TGGCCA 1 cut(s) 83
Mph1103I ATGCAT 1 cut(s) 250
MroI TCCGGA 1 cut(s) 40
MroNI GCCGGC 1 cut(s) 5
MscI TGGCCA 1 cut(s) 83
MseI TTAA 1 cut(s) 119
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 4 cut(s) 6, 30, 41, 443
MspR9I CCNGG 2 cut(s) 31, 288
MvaI CCWGG 1 cut(s) 288
MvnI CGCG 1 cut(s) 53
MwoI GCNNNNNNNGC 4 cut(s) 109, 148, 227, 329
NaeI GCCGGC 1 cut(s) 7
NciI CCSGG 1 cut(s) 31
NcoI CCATGG 1 cut(s) 378
NdeII GATC 1 cut(s) 369
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 5 cut(s) 75, 111, 142, 382, 396
NlaIV GGNNCC 3 cut(s) 113, 175, 348
NmuCI GTSAC 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 250
NspI RCATGY 1 cut(s) 142
PdiI GCCGGC 1 cut(s) 7
PfeI GAWTC 3 cut(s) 44, 240, 389
PinAI ACCGGT 1 cut(s) 442
PkrI GCNGC 2 cut(s) 102, 476
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
PpuMI RGGWCCY 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 1 cut(s) 286
PspGI CCWGG 1 cut(s) 286
PspN4I GGNNCC 3 cut(s) 113, 175, 348
PspPI GGNCC 3 cut(s) 111, 346, 479
PspPPI RGGWCCY 1 cut(s) 346
RsaI GTAC 1 cut(s) 175
RsaNI GTAC 1 cut(s) 174
RseI CAYNNNNRTG 1 cut(s) 137
SacII CCGCGG 1 cut(s) 54
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 2 cut(s) 101, 475
Sau3AI GATC 1 cut(s) 369
Sau96I GGNCC 3 cut(s) 111, 346, 479
SchI GAGTC 1 cut(s) 444
ScrFI CCNGG 2 cut(s) 31, 288
SetI ASST 3 cut(s) 179, 223, 315
Sfr303I CCGCGG 1 cut(s) 54
SgrBI CCGCGG 1 cut(s) 54
SinI GGWCC 2 cut(s) 346, 479
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
Sse9I AATT 4 cut(s) 120, 143, 292, 483
SsiI CCGC 5 cut(s) 51, 53, 101, 474, 477
StyD4I CCNGG 2 cut(s) 29, 286
StyI CCWWGG 1 cut(s) 378
TaaI ACNGT 1 cut(s) 173
TaqI TCGA 3 cut(s) 238, 460, 467
TaqII GACCGA 1 cut(s) 170
TasI AATT 4 cut(s) 120, 143, 292, 483
TauI GCSGC 2 cut(s) 103, 477
TfiI GAWTC 3 cut(s) 44, 240, 389
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 1 cut(s) 230
TseFI GTSAC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 2 cut(s) 60, 381
TspGWI ACGGA 1 cut(s) 218
TspRI CASTG 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 346, 479
XceI RCATGY 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
Zsp2I ATGCAT 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.