Rh4CG093600

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000345
Physical Location & Seq
Forward (+)
16962004 .. 16963293
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG093600.1

Sequence Viewer

Length: 720 bp
ATGAGAACACCAGCAGTCTGTCTTATTTTCTTGCTCTCTGTTAGTCTGTTCAAGGGTTCCCATGCAGAGCCATACGAATACCTACAGTTTGTCTTGCAATACGCTAGAGGTTCCTGTGTTAACGTCAAGAAATGCATTCCACCGGCGAGCTTGCCGGCCAAATTCACGGTTCACGGAATTTGGCCCACGAATATTTCCAAGCAGGAGGTGATCGAGTGTACAGCTGCCGTGAAACTACACTCGTTCGACGGAAAACTGATAACACGGTCGCTGAAAACCGATCTTCTCCAATCTTGGCCCAGCGTCCGGAGAGATAAAGATAATATGACTTTTTGGGAACACGAATATAATAAGCATGGGTCCTGTACTGCACCAGCTATCACTCAGACAGCGTATTTTGAGAGAGCTCACAAGTTCTGGAAGGAGTATGATCTCTATTCTGTTTTGGAACAAAAAAAAATCAAGCCGGGGCAGTCTCGATGGTATTCACTGGCTGATTTTCAAGCAGCCGTCCAGTCCAAGATTAGTACCGATTATATACCTCTCATCCTGTGCAAGGAGGAAGCATTAACTCGTGGGAGCGGCACAACTGAATTAATTCTGAGGGAAATAGTTATCTGTTTCGATCACCAGGGGACAGATCTGGTCAATTGTACCCGGTCCACGGATTGCAAGATGATGAAGAACACAACTGCAACCAATAACATATACTACGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

239

Amino Acids

27.32

Weight (kDa)

8.77

Isoelectric Point (pI)

53.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 25 - 218 8.8e-38 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 582
AccIII TCCGGA 1 cut(s) 306
AciI CCGC 1 cut(s) 582
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 161, 177
AfaI GTAC 4 cut(s) 220, 367, 529, 655
AfiI CCNNNNNNNGG 3 cut(s) 306, 556, 664
AgsI TTSAA 2 cut(s) 52, 503
AjnI CCWGG 1 cut(s) 630
AluBI AGCT 4 cut(s) 150, 224, 377, 407
AluI AGCT 4 cut(s) 150, 224, 377, 407
Alw21I GWGCWC 1 cut(s) 409
Alw26I GTCTC 1 cut(s) 480
Aor13HI TCCGGA 1 cut(s) 306
AoxI GGCC 3 cut(s) 156, 182, 296
ApeKI GCWGC 2 cut(s) 224, 506
ApoI RAATTY 2 cut(s) 161, 177
AseI ATTAAT 1 cut(s) 596
Asp700I GAANNNNTTC 1 cut(s) 597
AspS9I GGNCC 4 cut(s) 183, 297, 360, 660
AsuC2I CCSGG 2 cut(s) 468, 658
AsuHPI GGTGA 2 cut(s) 220, 620
AvaII GGWCC 2 cut(s) 360, 660
BanII GRGCYC 1 cut(s) 409
BauI CACGAG 1 cut(s) 573
Bbv12I GWGCWC 1 cut(s) 409
BbvI GCAGC 2 cut(s) 211, 518
BccI CCATC 1 cut(s) 474
BceAI ACGGC 2 cut(s) 212, 494
BciT130I CCWGG 1 cut(s) 632
BcnI CCSGG 2 cut(s) 468, 658
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 83
BglII AGATCT 1 cut(s) 640
BisI GCNGC 3 cut(s) 225, 507, 583
BlsI GCNGC 3 cut(s) 226, 508, 584
Bme1390I CCNGG 3 cut(s) 468, 632, 658
Bme18I GGWCC 2 cut(s) 360, 660
BmgT120I GGNCC 4 cut(s) 183, 297, 360, 660
BmiI GGNNCC 3 cut(s) 58, 112, 361
BmrFI CCNGG 3 cut(s) 468, 632, 658
BpuMI CCSGG 2 cut(s) 468, 658
BsaJI CCNNGG 3 cut(s) 467, 631, 663
BsaWI WCCGGW 1 cut(s) 306
Bsc4I CCNNNNNNNGG 3 cut(s) 306, 556, 664
Bse118I RCCGGY 2 cut(s) 142, 154
Bse1I ACTGG 2 cut(s) 495, 514
BseAI TCCGGA 1 cut(s) 306
BseBI CCWGG 1 cut(s) 632
BseDI CCNNGG 3 cut(s) 467, 631, 663
BseGI GGATG 1 cut(s) 546
BseLI CCNNNNNNNGG 3 cut(s) 306, 556, 664
BseMII CTCAG 2 cut(s) 398, 593
BseNI ACTGG 2 cut(s) 495, 514
BseXI GCAGC 2 cut(s) 211, 518
BseYI CCCAGC 1 cut(s) 299
BsgI GTGCAG 1 cut(s) 354
Bsh1285I CGRYCG 1 cut(s) 269
BshFI GGCC 3 cut(s) 158, 184, 298
BsiEI CGRYCG 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 409
BsiSI CCGG 5 cut(s) 143, 155, 307, 467, 658
BslFI GGGAC 1 cut(s) 649
BslI CCNNNNNNNGG 3 cut(s) 306, 556, 664
BsmAI GTCTC 1 cut(s) 480
BsmFI GGGAC 1 cut(s) 649
BsmI GAATGC 1 cut(s) 135
BsnI GGCC 3 cut(s) 158, 184, 298
Bsp1286I GDGCHC 1 cut(s) 409
Bsp13I TCCGGA 1 cut(s) 306
Bsp1407I TGTACA 1 cut(s) 218
Bsp143I GATC 5 cut(s) 210, 280, 430, 625, 640
BspACI CCGC 1 cut(s) 582
BspANI GGCC 3 cut(s) 158, 184, 298
BspCNI CTCAG 2 cut(s) 397, 594
BspEI TCCGGA 1 cut(s) 306
BspLI GGNNCC 3 cut(s) 58, 112, 361
BsrBI CCGCTC 1 cut(s) 582
BsrFI RCCGGY 2 cut(s) 142, 154
BsrGI TGTACA 1 cut(s) 218
BsrI ACTGG 2 cut(s) 495, 514
BssAI RCCGGY 2 cut(s) 142, 154
BssECI CCNNGG 3 cut(s) 467, 631, 663
BssMI GATC 5 cut(s) 210, 280, 430, 625, 640
BssSI CACGAG 1 cut(s) 573
Bst2BI CACGAG 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 3 cut(s) 87, 169, 267
BstAUI TGTACA 1 cut(s) 218
BstC8I GCNNGC 3 cut(s) 148, 152, 156
BstDEI CTNAG 2 cut(s) 384, 602
BstDSI CCRYGG 1 cut(s) 663
BstENI CCTNNNNNAGG 1 cut(s) 554
BstF5I GGATG 1 cut(s) 546
BstKTI GATC 5 cut(s) 213, 283, 433, 628, 643
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 5 cut(s) 210, 280, 430, 625, 640
BstMCI CGRYCG 1 cut(s) 269
BstNI CCWGG 1 cut(s) 632
BstSCI CCNGG 3 cut(s) 466, 630, 656
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 211, 518
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
BsuRI GGCC 3 cut(s) 158, 184, 298
BtgI CCRYGG 1 cut(s) 663
BtsCI GGATG 1 cut(s) 546
BtsIMutI CAGTG 1 cut(s) 488
Cac8I GCNNGC 3 cut(s) 148, 152, 156
Cfr10I RCCGGY 2 cut(s) 142, 154
Cfr13I GGNCC 4 cut(s) 183, 297, 360, 660
CseI GACGC 1 cut(s) 292
Csp6I GTAC 4 cut(s) 219, 366, 528, 654
CviAII CATG 2 cut(s) 62, 356
CviQI GTAC 4 cut(s) 219, 366, 528, 654
DdeI CTNAG 2 cut(s) 384, 602
DpnI GATC 5 cut(s) 212, 282, 432, 627, 642
DpnII GATC 5 cut(s) 210, 280, 430, 625, 640
EaeI YGGCCR 1 cut(s) 156
Ecl136II GAGCTC 1 cut(s) 407
Eco24I GRGCYC 1 cut(s) 409
Eco47I GGWCC 2 cut(s) 360, 660
Eco53kI GAGCTC 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 407
EcoNI CCTNNNNNAGG 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 360
EcoRII CCWGG 1 cut(s) 630
EcoT22I ATGCAT 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 409
FaeI CATG 2 cut(s) 65, 359
FaqI GGGAC 1 cut(s) 649
FatI CATG 2 cut(s) 61, 355
Fnu4HI GCNGC 3 cut(s) 225, 507, 583
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 409
Fsp4HI GCNGC 3 cut(s) 225, 507, 583
FspBI CTAG 1 cut(s) 105
GluI GCNGC 3 cut(s) 225, 507, 583
GsaI CCCAGC 1 cut(s) 303
HaeIII GGCC 3 cut(s) 158, 184, 298
HapII CCGG 5 cut(s) 143, 155, 307, 467, 658
HgaI GACGC 1 cut(s) 292
Hin1II CATG 2 cut(s) 65, 359
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HpaI GTTAAC 1 cut(s) 121
HpaII CCGG 5 cut(s) 143, 155, 307, 467, 658
HphI GGTGA 2 cut(s) 220, 620
Hpy166II GTNNAC 4 cut(s) 121, 172, 219, 663
Hpy188I TCNGA 2 cut(s) 387, 603
Hpy188III TCNNGA 4 cut(s) 127, 307, 418, 477
Hpy8I GTNNAC 4 cut(s) 121, 172, 219, 663
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 415
HpyCH4III ACNGT 3 cut(s) 87, 169, 267
HpyCH4IV ACGT 2 cut(s) 123, 714
HpyCH4V TGCA 7 cut(s) 65, 97, 135, 371, 555, 672, 695
HpyF3I CTNAG 2 cut(s) 384, 602
HpySE526I ACGT 2 cut(s) 123, 714
Hsp92II CATG 2 cut(s) 65, 359
Kpn2I TCCGGA 1 cut(s) 306
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 5 cut(s) 210, 280, 430, 625, 640
LmnI GCTCC 1 cut(s) 579
Lsp1109I GCAGC 2 cut(s) 211, 518
MaeI CTAG 1 cut(s) 105
MaeII ACGT 2 cut(s) 123, 714
MalI GATC 5 cut(s) 212, 282, 432, 627, 642
MbiI CCGCTC 1 cut(s) 582
MboI GATC 5 cut(s) 210, 280, 430, 625, 640
MboII GAAGA 2 cut(s) 275, 694
MfeI CAATTG 1 cut(s) 649
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 409
MluCI AATT 5 cut(s) 161, 177, 593, 597, 649
MnlI CCTC 5 cut(s) 101, 199, 552, 553, 597
Mph1103I ATGCAT 1 cut(s) 137
MroI TCCGGA 1 cut(s) 306
MroNI GCCGGC 1 cut(s) 154
MroXI GAANNNNTTC 1 cut(s) 597
MseI TTAA 3 cut(s) 120, 569, 596
MspA1I CMGCKG 1 cut(s) 224
MspI CCGG 5 cut(s) 143, 155, 307, 467, 658
MspR9I CCNGG 3 cut(s) 468, 632, 658
MunI CAATTG 1 cut(s) 649
Mva1269I GAATGC 1 cut(s) 135
MvaI CCWGG 1 cut(s) 632
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 2 cut(s) 468, 658
NdeII GATC 5 cut(s) 210, 280, 430, 625, 640
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 2 cut(s) 65, 359
NlaIV GGNNCC 3 cut(s) 58, 112, 361
NsiI ATGCAT 1 cut(s) 137
PctI GAATGC 1 cut(s) 135
PdiI GCCGGC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 597
PkrI GCNGC 3 cut(s) 226, 508, 584
PpuMI RGGWCCY 1 cut(s) 360
PshBI ATTAAT 1 cut(s) 596
Psp124BI GAGCTC 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 630
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 1 cut(s) 630
PspN4I GGNNCC 3 cut(s) 58, 112, 361
PspPI GGNCC 4 cut(s) 183, 297, 360, 660
PspPPI RGGWCCY 1 cut(s) 360
PsuI RGATCY 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 4 cut(s) 220, 367, 529, 655
RsaNI GTAC 4 cut(s) 219, 366, 528, 654
SacI GAGCTC 1 cut(s) 409
SaqAI TTAA 3 cut(s) 120, 569, 596
SatI GCNGC 3 cut(s) 225, 507, 583
Sau3AI GATC 5 cut(s) 210, 280, 430, 625, 640
Sau96I GGNCC 4 cut(s) 183, 297, 360, 660
ScrFI CCNGG 3 cut(s) 468, 632, 658
SduI GDGCHC 1 cut(s) 409
SfcI CTRYAG 1 cut(s) 83
SgrAI CRCCGGYG 1 cut(s) 142
SinI GGWCC 2 cut(s) 360, 660
Sse9I AATT 5 cut(s) 161, 177, 593, 597, 649
SsiI CCGC 1 cut(s) 582
SspI AATATT 1 cut(s) 193
SspMI CTAG 1 cut(s) 105
SstI GAGCTC 1 cut(s) 409
StyD4I CCNGG 3 cut(s) 466, 630, 656
TaaI ACNGT 3 cut(s) 87, 169, 267
TaiI ACGT 2 cut(s) 126, 717
TaqI TCGA 4 cut(s) 213, 246, 478, 624
TasI AATT 5 cut(s) 161, 177, 593, 597, 649
TatI WGTACW 2 cut(s) 218, 365
TauI GCSGC 1 cut(s) 585
Tru1I TTAA 3 cut(s) 120, 569, 596
Tru9I TTAA 3 cut(s) 120, 569, 596
TscAI CASTG 1 cut(s) 495
TseI GCWGC 2 cut(s) 224, 506
TspDTI ATGAA 1 cut(s) 695
TspGWI ACGGA 3 cut(s) 189, 264, 680
TspRI CASTG 1 cut(s) 495
VpaK11BI GGWCC 2 cut(s) 360, 660
VspI ATTAAT 1 cut(s) 596
XagI CCTNNNNNAGG 1 cut(s) 554
XapI RAATTY 2 cut(s) 161, 177
XmnI GAANNNNTTC 1 cut(s) 597
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.