FvH4_6g08450

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000345
Physical Location & Seq
Reverse (-)
5008339 .. 5008749
411 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08450.t1

Sequence Viewer

Length: 315 bp
ATGAAAACCCCAGGAATGTGTCTCGTTTTCTTCTTGCTCTCTGATGTTCTATTCATCAAGGATTCCAAAGCGGAATCTTACGAATACCTTCAGTTTGTGCTGCAGTACCCGCGAGGTACCTGTGTTAACGATAAGACATGCAAGCCGACGGCGAGCTTGCCGGCGAAGTTCGCTATGCATGGGATTTGGCCCACGAATATATCCGCGACGTACATCAAATGTATACAGGCAGTGAAACAATACCCGTTCGACAAAAACCTGATAACTCCTTCGCTCAAAGCTGATCTCCTCCAATCTTGGCGTCACGACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

105

Amino Acids

11.89

Weight (kDa)

8.93

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 101 8.1e-09 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 116
AccB1I GGYRCC 1 cut(s) 116
AccI GTMKAC 1 cut(s) 223
AccII CGCG 2 cut(s) 112, 206
AciI CCGC 3 cut(s) 71, 110, 204
AcuI CTGAAG 1 cut(s) 74
AcyI GRCGYC 1 cut(s) 301
AfaI GTAC 3 cut(s) 107, 118, 212
AjnI CCWGG 1 cut(s) 10
AluBI AGCT 2 cut(s) 156, 281
AluI AGCT 2 cut(s) 156, 281
Alw26I GTCTC 1 cut(s) 26
AoxI GGCC 1 cut(s) 188
ApeKI GCWGC 1 cut(s) 100
Asp700I GAANNNNTTC 1 cut(s) 87
Asp718I GGTACC 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 189
BaeI ACNNNNGTAYC 2 cut(s) 108, 141
BanI GGYRCC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 87
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 1 cut(s) 12
BcoDI GTCTC 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
Bme1390I CCNGG 1 cut(s) 12
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 10
Bse118I RCCGGY 1 cut(s) 160
BseBI CCWGG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 10
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 1 cut(s) 87
Bsh1236I CGCG 2 cut(s) 112, 206
BshFI GGCC 1 cut(s) 190
BshNI GGYRCC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 161
BsmAI GTCTC 1 cut(s) 26
BsnI GGCC 1 cut(s) 190
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 3 cut(s) 71, 110, 204
BspANI GGCC 1 cut(s) 190
BspFNI CGCG 2 cut(s) 112, 206
BspLI GGNNCC 1 cut(s) 118
BspMAI CTGCAG 1 cut(s) 105
BspT107I GGYRCC 1 cut(s) 116
BsrFI RCCGGY 1 cut(s) 160
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 1 cut(s) 283
BssNAI GTATAC 1 cut(s) 224
BssNI GRCGYC 1 cut(s) 301
Bst1107I GTATAC 1 cut(s) 224
Bst2UI CCWGG 1 cut(s) 12
BstACI GRCGYC 1 cut(s) 301
BstC8I GCNNGC 4 cut(s) 143, 154, 158, 162
BstFNI CGCG 2 cut(s) 112, 206
BstKTI GATC 1 cut(s) 286
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 2 cut(s) 109, 170
BstNI CCWGG 1 cut(s) 12
BstNSI RCATGY 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 10
BstSFI CTRYAG 1 cut(s) 101
BstUI CGCG 2 cut(s) 112, 206
BstV1I GCAGC 1 cut(s) 87
BstZ17I GTATAC 1 cut(s) 224
BsuRI GGCC 1 cut(s) 190
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 4 cut(s) 143, 154, 158, 162
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 189
CseI GACGC 1 cut(s) 290
Csp6I GTAC 3 cut(s) 106, 117, 211
CviAII CATG 2 cut(s) 138, 179
CviJI RGCY 4 cut(s) 145, 156, 190, 281
CviKI_1 RGCY 4 cut(s) 145, 156, 190, 281
CviQI GTAC 3 cut(s) 106, 117, 211
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
Eco57I CTGAAG 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 10
EcoT22I ATGCAT 1 cut(s) 180
FaeI CATG 2 cut(s) 141, 182
FaiI YATR 5 cut(s) 139, 176, 180, 200, 224
FatI CATG 2 cut(s) 137, 178
FauI CCCGC 1 cut(s) 117
FblI GTMKAC 1 cut(s) 223
Fnu4HI GCNGC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 101
GluI GCNGC 1 cut(s) 101
HaeIII GGCC 1 cut(s) 190
HapII CCGG 1 cut(s) 161
HgaI GACGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 301
Hin1II CATG 2 cut(s) 141, 182
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 2 cut(s) 62, 74
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 127, 224
Hpy188I TCNGA 1 cut(s) 43
Hpy188III TCNNGA 1 cut(s) 305
Hpy8I GTNNAC 2 cut(s) 127, 224
Hpy99I CGWCG 2 cut(s) 151, 211
HpyAV CCTTC 2 cut(s) 98, 279
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 3 cut(s) 103, 141, 178
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 170
HpySE526I ACGT 1 cut(s) 209
Hsp92I GRCGYC 1 cut(s) 301
Hsp92II CATG 2 cut(s) 141, 182
KpnI GGTACC 1 cut(s) 120
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 1 cut(s) 283
LpnPI CCDG 5 cut(s) 24, 133, 174, 212, 272
Lsp1109I GCAGC 1 cut(s) 87
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 1 cut(s) 302
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 1 cut(s) 22
MnlI CCTC 2 cut(s) 107, 299
Mph1103I ATGCAT 1 cut(s) 180
MroNI GCCGGC 1 cut(s) 160
MroXI GAANNNNTTC 1 cut(s) 87
MseI TTAA 1 cut(s) 126
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 12
MvnI CGCG 2 cut(s) 112, 206
MwoI GCNNNNNNNGC 2 cut(s) 109, 170
NaeI GCCGGC 1 cut(s) 162
NdeII GATC 1 cut(s) 283
NgoMIV GCCGGC 1 cut(s) 160
NlaIII CATG 2 cut(s) 141, 182
NlaIV GGNNCC 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 180
NspI RCATGY 1 cut(s) 141
PdiI GCCGGC 1 cut(s) 162
PdmI GAANNNNTTC 1 cut(s) 87
PfeI GAWTC 2 cut(s) 62, 74
PkrI GCNGC 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 10
PspGI CCWGG 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 189
PstI CTGCAG 1 cut(s) 105
RsaI GTAC 3 cut(s) 107, 118, 212
RsaNI GTAC 3 cut(s) 106, 117, 211
SaqAI TTAA 1 cut(s) 126
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 1 cut(s) 283
Sau96I GGNCC 1 cut(s) 189
ScrFI CCNGG 1 cut(s) 12
SetI ASST 7 cut(s) 90, 118, 122, 158, 212, 261, 283
SfcI CTRYAG 1 cut(s) 101
SsiI CCGC 3 cut(s) 71, 110, 204
StyD4I CCNGG 1 cut(s) 10
TaiI ACGT 1 cut(s) 212
TaqI TCGA 1 cut(s) 249
TfiI GAWTC 2 cut(s) 62, 74
Tru1I TTAA 1 cut(s) 126
Tru9I TTAA 1 cut(s) 126
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 1 cut(s) 100
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 2 cut(s) 17, 43
TspRI CASTG 1 cut(s) 237
XceI RCATGY 1 cut(s) 141
XmiI GTMKAC 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 87
Zsp2I ATGCAT 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.