Rw3G006100

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000345
Physical Location & Seq
Reverse (-)
5514505 .. 5514948
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G006100.1

Sequence Viewer

Length: 444 bp
ATGTTTTCAAATCAAACATTGGTAAACGACCTGACTCGCTCTTGGCCGGACCTGAGAAATGGGAACCATAGAAGGTTTTGGATAGAGCAGTGGGATAAACATGGCCAATGCTCACAACAGACATATACACAAACGCGATACTTCCAGCGCTCCATCGAGATGTGGAACCAATTCGATATTACTAATATCTTTACTGCCGCTGGCGTCGTATCACAGCCAGGGACGCAACCACGTATAGTCAACAAGAGCGACTTAGAAACACGCATTCAAGCAGTAACTCATGCGATACCTGTCCTTCGGTGCAGAAAAGCTACAGTGCAGAACGTTCAACAGCCAGTTGATTTAATTTATGAAGTGGGCATTTGTTACGATTATCTTGGAACAAATCTGTTTCCTTGTAATCCCAAACAAGGAAAATGCACCGGAAAAGATATTTACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

147

Amino Acids

17.15

Weight (kDa)

8.88

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 5 - 133 3.2e-21 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 136
AciI CCGC 1 cut(s) 198
AclI AACGTT 1 cut(s) 324
AcoI YGGCCR 2 cut(s) 44, 103
AcyI GRCGYC 1 cut(s) 204
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 410
AgsI TTSAA 3 cut(s) 9, 269, 329
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 1 cut(s) 311
AluI AGCT 1 cut(s) 311
Aor51HI AGCGCT 1 cut(s) 149
AoxI GGCC 2 cut(s) 44, 103
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 150
AspS9I GGNCC 1 cut(s) 49
AvaII GGWCC 1 cut(s) 49
BalI TGGCCA 1 cut(s) 105
BccI CCATC 1 cut(s) 161
BciT130I CCWGG 1 cut(s) 219
BfmI CTRYAG 1 cut(s) 312
BfoI RGCGCY 1 cut(s) 151
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 1 cut(s) 219
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 2 cut(s) 65, 167
BmrFI CCNGG 1 cut(s) 219
BsaAI YACGTR 1 cut(s) 233
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 218
BsaWI WCCGGW 1 cut(s) 422
Bsc4I CCNNNNNNNGG 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 335
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 218
BseLI CCNNNNNNNGG 1 cut(s) 410
BseMII CTCAG 1 cut(s) 44
BseNI ACTGG 1 cut(s) 335
BsgI GTGCAG 2 cut(s) 322, 338
Bsh1236I CGCG 1 cut(s) 136
BshFI GGCC 2 cut(s) 46, 105
BsiSI CCGG 2 cut(s) 47, 423
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 1 cut(s) 410
BsmFI GGGAC 1 cut(s) 235
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 2 cut(s) 46, 105
BspACI CCGC 1 cut(s) 198
BspANI GGCC 2 cut(s) 46, 105
BspCNI CTCAG 1 cut(s) 45
BspFNI CGCG 1 cut(s) 136
BspLI GGNNCC 2 cut(s) 65, 167
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 1 cut(s) 218
BssNI GRCGYC 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 316
BstACI GRCGYC 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 2 cut(s) 53, 253
BstFNI CGCG 1 cut(s) 136
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 1 cut(s) 150
BstMWI GCNNNNNNNGC 1 cut(s) 223
BstNI CCWGG 1 cut(s) 219
BstSCI CCNGG 1 cut(s) 217
BstSFI CTRYAG 1 cut(s) 312
BstUI CGCG 1 cut(s) 136
BsuRI GGCC 2 cut(s) 46, 105
BtsI GCAGTG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 95, 321
Cac8I GCNNGC 1 cut(s) 202
CfoI GCGC 1 cut(s) 150
Cfr13I GGNCC 1 cut(s) 49
CseI GACGC 2 cut(s) 193, 232
CviAII CATG 2 cut(s) 101, 281
CviJI RGCY 5 cut(s) 46, 105, 217, 311, 334
CviKI_1 RGCY 5 cut(s) 46, 105, 217, 311, 334
DdeI CTNAG 2 cut(s) 53, 253
EaeI YGGCCR 2 cut(s) 44, 103
Eco47I GGWCC 1 cut(s) 49
Eco47III AGCGCT 1 cut(s) 149
EcoRII CCWGG 1 cut(s) 217
FaeI CATG 2 cut(s) 104, 284
FaiI YATR 7 cut(s) 69, 102, 124, 126, 236, 282, 351
FalI AAGNNNNNCTT 2 cut(s) 236, 268
FaqI GGGAC 1 cut(s) 235
FatI CATG 2 cut(s) 100, 280
Fnu4HI GCNGC 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 198
GlaI GCGC 1 cut(s) 149
GluI GCNGC 1 cut(s) 198
HaeII RGCGCY 1 cut(s) 151
HaeIII GGCC 2 cut(s) 46, 105
HapII CCGG 2 cut(s) 47, 423
HgaI GACGC 2 cut(s) 193, 232
HhaI GCGC 1 cut(s) 150
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 104, 284
Hin6I GCGC 1 cut(s) 148
HinP1I GCGC 1 cut(s) 148
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 1 cut(s) 34
HpaII CCGG 2 cut(s) 47, 423
Hpy166II GTNNAC 2 cut(s) 25, 241
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 2 cut(s) 25, 241
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 66, 305
HpyCH4III ACNGT 1 cut(s) 316
HpyCH4IV ACGT 2 cut(s) 232, 324
HpyCH4V TGCA 3 cut(s) 303, 319, 420
HpyF10VI GCNNNNNNNGC 1 cut(s) 223
HpyF3I CTNAG 2 cut(s) 53, 253
HpySE526I ACGT 2 cut(s) 232, 324
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 104, 284
HspAI GCGC 1 cut(s) 148
LmnI GCTCC 1 cut(s) 155
MaeII ACGT 2 cut(s) 232, 324
MaeIII GTNAC 2 cut(s) 274, 365
MlsI TGGCCA 1 cut(s) 105
MluCI AATT 2 cut(s) 170, 345
MluNI TGGCCA 1 cut(s) 105
MlyI GAGTC 1 cut(s) 28
Mox20I TGGCCA 1 cut(s) 105
MroXI GAANNNNTTC 1 cut(s) 170
MscI TGGCCA 1 cut(s) 105
MseI TTAA 1 cut(s) 344
MslI CAYNNNNRTG 1 cut(s) 158
Msp20I TGGCCA 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 2 cut(s) 47, 423
MspR9I CCNGG 1 cut(s) 219
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 1 cut(s) 219
MvnI CGCG 1 cut(s) 136
MwoI GCNNNNNNNGC 1 cut(s) 223
NlaIII CATG 2 cut(s) 104, 284
NlaIV GGNNCC 2 cut(s) 65, 167
PctI GAATGC 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 170
PkrI GCNGC 1 cut(s) 199
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
Ppu21I YACGTR 1 cut(s) 233
Psp1406I AACGTT 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 217
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 65, 167
PspPI GGNCC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 1 cut(s) 344
SatI GCNGC 1 cut(s) 198
Sau96I GGNCC 1 cut(s) 49
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 1 cut(s) 219
SetI ASST 7 cut(s) 33, 54, 77, 235, 292, 313, 327
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 2 cut(s) 170, 345
SsiI CCGC 1 cut(s) 198
StyD4I CCNGG 1 cut(s) 217
TaaI ACNGT 1 cut(s) 316
TaiI ACGT 2 cut(s) 235, 327
TaqI TCGA 2 cut(s) 156, 174
TasI AATT 2 cut(s) 170, 345
TauI GCSGC 1 cut(s) 200
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TscAI CASTG 2 cut(s) 95, 321
TspDTI ATGAA 1 cut(s) 366
TspRI CASTG 2 cut(s) 95, 321
VpaK11BI GGWCC 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.