Rh2AG305200

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000345
Physical Location & Seq
Reverse (-)
38401218 .. 38402288
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG305200.1

Sequence Viewer

Length: 651 bp
ATGCAGAGCCATACGAATACCTACAGTTTGTCTTGCAATACCCTAGAGGTTACTGTACGTGTTAACGACAAGAAATGCATTCCACCGGCGAGCTTGCCAGCCAAATTCACGGTTCATGGAATTTGGCCCACGAATATTTCCGAGCCGATTATCAGCTGTGACAAGGCACAGAAACTACACTCGTTCAACGGAAACCTGATAACTCCGTCGTTGAAAACCGATCTTCTCCAATCTTGGCCCAGCGTCGTGACAAATAAAGATGTTATGTCGTTTTGGGAACACGAATATATTAAGCATGGGTCCTGTACTGCACCAGCTATCACTCAGACAGCGTATTTTGAGAGAGCTCACAAGTTCTGGAAGGAGTATGATCTCTATTCCATTTTGGAACAAAAAAATATCAAGCCGGGGCAATCTCGATGGTATTCACTGGCTGATTTTGAAGCAGCCGTCAAGTCCAAGATCGGTACCGACACAAAACCTCTCATCCTTTGTAAGCAGGACAAACTAACTCGTGGGAGCACAACAACCAACTTCATTTTGAGGGAAATTGTGATCTGTTTCGATCACCAGGGGACAAATCTGGTCAATTATATTGTACCCGATCAACAGATTGTGACCAGAAGCAGAAGATATACTACCCTTAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

216

Amino Acids

24.64

Weight (kDa)

8.97

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 23 - 197 1.5e-34 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AcsI RAATTY 2 cut(s) 104, 120
AfaI GTAC 4 cut(s) 57, 307, 469, 600
AflIII ACRYGT 1 cut(s) 58
AgsI TTSAA 3 cut(s) 187, 214, 443
AjnI CCWGG 1 cut(s) 570
AluBI AGCT 5 cut(s) 93, 156, 317, 347, 648
AluI AGCT 5 cut(s) 93, 156, 317, 347, 648
Alw21I GWGCWC 2 cut(s) 349, 524
AoxI GGCC 2 cut(s) 125, 236
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 2 cut(s) 104, 120
Asp718I GGTACC 1 cut(s) 467
AspS9I GGNCC 3 cut(s) 126, 237, 300
AsuC2I CCSGG 1 cut(s) 408
AsuHPI GGTGA 1 cut(s) 560
AvaII GGWCC 1 cut(s) 300
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 349
BarI GAAGNNNNNNTAC 1 cut(s) 622
BauI CACGAG 1 cut(s) 513
Bbv12I GWGCWC 2 cut(s) 349, 524
BbvI GCAGC 1 cut(s) 458
BccI CCATC 1 cut(s) 414
BceAI ACGGC 1 cut(s) 434
BciT130I CCWGG 1 cut(s) 572
BcnI CCSGG 1 cut(s) 408
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
Bme1390I CCNGG 2 cut(s) 408, 572
Bme18I GGWCC 1 cut(s) 300
BmgT120I GGNCC 3 cut(s) 126, 237, 300
BmiI GGNNCC 2 cut(s) 301, 469
BmrFI CCNGG 2 cut(s) 408, 572
Bpu10I CCTNAGC 1 cut(s) 644
BpuMI CCSGG 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 407, 571
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 1 cut(s) 435
BseBI CCWGG 1 cut(s) 572
BseDI CCNNGG 2 cut(s) 407, 571
BseGI GGATG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 338
BseNI ACTGG 1 cut(s) 435
BseXI GCAGC 1 cut(s) 458
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 1 cut(s) 294
BshFI GGCC 2 cut(s) 127, 238
BshNI GGYRCC 1 cut(s) 467
BsiHKAI GWGCWC 2 cut(s) 349, 524
BsiSI CCGG 2 cut(s) 86, 407
BslFI GGGAC 1 cut(s) 589
BsmFI GGGAC 1 cut(s) 589
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 2 cut(s) 127, 238
Bsp1286I GDGCHC 2 cut(s) 349, 524
Bsp143I GATC 6 cut(s) 220, 370, 462, 555, 565, 604
BspANI GGCC 2 cut(s) 127, 238
BspCNI CTCAG 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 301, 469
BspT107I GGYRCC 1 cut(s) 467
BsrFI RCCGGY 1 cut(s) 85
BsrI ACTGG 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 407, 571
BssMI GATC 6 cut(s) 220, 370, 462, 555, 565, 604
BssSI CACGAG 1 cut(s) 513
Bst2BI CACGAG 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 3 cut(s) 26, 55, 112
BstBAI YACGTR 1 cut(s) 59
BstC8I GCNNGC 3 cut(s) 91, 95, 99
BstDEI CTNAG 2 cut(s) 324, 644
BstF5I GGATG 1 cut(s) 486
BstKTI GATC 6 cut(s) 223, 373, 465, 558, 568, 607
BstMBI GATC 6 cut(s) 220, 370, 462, 555, 565, 604
BstNI CCWGG 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 406, 570
BstSFI CTRYAG 1 cut(s) 22
BstV1I GCAGC 1 cut(s) 458
BsuRI GGCC 2 cut(s) 127, 238
BtsCI GGATG 1 cut(s) 486
BtsIMutI CAGTG 1 cut(s) 428
Cac8I GCNNGC 3 cut(s) 91, 95, 99
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 3 cut(s) 126, 237, 300
CseI GACGC 1 cut(s) 232
Csp6I GTAC 4 cut(s) 56, 306, 468, 599
CviAII CATG 2 cut(s) 116, 296
CviQI GTAC 4 cut(s) 56, 306, 468, 599
DdeI CTNAG 2 cut(s) 324, 644
DpnI GATC 6 cut(s) 222, 372, 464, 557, 567, 606
DpnII GATC 6 cut(s) 220, 370, 462, 555, 565, 604
Ecl136II GAGCTC 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 300
Eco53kI GAGCTC 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 300
EcoRII CCWGG 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 349
FaeI CATG 2 cut(s) 119, 299
FaiI YATR 8 cut(s) 12, 117, 266, 288, 297, 369, 594, 636
FaqI GGGAC 1 cut(s) 589
FatI CATG 2 cut(s) 115, 295
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 1 cut(s) 473
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 1 cut(s) 44
GluI GCNGC 1 cut(s) 447
GsaI CCCAGC 1 cut(s) 243
HaeIII GGCC 2 cut(s) 127, 238
HapII CCGG 2 cut(s) 86, 407
HgaI GACGC 1 cut(s) 232
Hin1II CATG 2 cut(s) 119, 299
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HpaI GTTAAC 1 cut(s) 64
HpaII CCGG 2 cut(s) 86, 407
HphI GGTGA 1 cut(s) 560
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 2 cut(s) 142, 327
Hpy188III TCNNGA 3 cut(s) 247, 358, 417
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 2 cut(s) 211, 248
HpyAV CCTTC 1 cut(s) 355
HpyCH4III ACNGT 3 cut(s) 26, 55, 112
HpyCH4IV ACGT 1 cut(s) 58
HpyCH4V TGCA 4 cut(s) 4, 36, 78, 311
HpyF3I CTNAG 2 cut(s) 324, 644
HpySE526I ACGT 1 cut(s) 58
Hsp92II CATG 2 cut(s) 119, 299
KpnI GGTACC 1 cut(s) 471
KspAI GTTAAC 1 cut(s) 64
Kzo9I GATC 6 cut(s) 220, 370, 462, 555, 565, 604
LmnI GCTCC 1 cut(s) 519
Lsp1109I GCAGC 1 cut(s) 458
MaeI CTAG 1 cut(s) 44
MaeII ACGT 1 cut(s) 58
MaeIII GTNAC 4 cut(s) 49, 158, 247, 616
MalI GATC 6 cut(s) 222, 372, 464, 557, 567, 606
MboI GATC 6 cut(s) 220, 370, 462, 555, 565, 604
MboII GAAGA 2 cut(s) 215, 642
MhlI GDGCHC 2 cut(s) 349, 524
MluCI AATT 4 cut(s) 104, 120, 549, 589
MnlI CCTC 3 cut(s) 40, 492, 537
Mph1103I ATGCAT 1 cut(s) 80
MseI TTAA 2 cut(s) 63, 291
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 2 cut(s) 86, 407
MspR9I CCNGG 2 cut(s) 408, 572
Mva1269I GAATGC 1 cut(s) 78
MvaI CCWGG 1 cut(s) 572
NciI CCSGG 1 cut(s) 408
NdeII GATC 6 cut(s) 220, 370, 462, 555, 565, 604
NlaIII CATG 2 cut(s) 119, 299
NlaIV GGNNCC 2 cut(s) 301, 469
NmuCI GTSAC 3 cut(s) 158, 247, 616
NsiI ATGCAT 1 cut(s) 80
PctI GAATGC 1 cut(s) 78
PkrI GCNGC 1 cut(s) 448
Ppu21I YACGTR 1 cut(s) 59
PpuMI RGGWCCY 1 cut(s) 300
Psp124BI GAGCTC 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 570
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 2 cut(s) 301, 469
PspPI GGNCC 3 cut(s) 126, 237, 300
PspPPI RGGWCCY 1 cut(s) 300
PvuII CAGCTG 1 cut(s) 156
RsaI GTAC 4 cut(s) 57, 307, 469, 600
RsaNI GTAC 4 cut(s) 56, 306, 468, 599
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 2 cut(s) 63, 291
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 6 cut(s) 220, 370, 462, 555, 565, 604
Sau96I GGNCC 3 cut(s) 126, 237, 300
ScrFI CCNGG 2 cut(s) 408, 572
SduI GDGCHC 2 cut(s) 349, 524
SfcI CTRYAG 1 cut(s) 22
SgrAI CRCCGGYG 1 cut(s) 85
SinI GGWCC 1 cut(s) 300
Sse9I AATT 4 cut(s) 104, 120, 549, 589
SspI AATATT 1 cut(s) 136
SspMI CTAG 1 cut(s) 44
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 2 cut(s) 406, 570
TaaI ACNGT 3 cut(s) 26, 55, 112
TaiI ACGT 1 cut(s) 61
TaqI TCGA 2 cut(s) 418, 564
TasI AATT 4 cut(s) 104, 120, 549, 589
TatI WGTACW 1 cut(s) 305
Tru1I TTAA 2 cut(s) 63, 291
Tru9I TTAA 2 cut(s) 63, 291
TscAI CASTG 1 cut(s) 435
TseFI GTSAC 3 cut(s) 158, 247, 616
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 3 cut(s) 158, 247, 616
TspDTI ATGAA 2 cut(s) 104, 526
TspGWI ACGGA 2 cut(s) 195, 204
TspRI CASTG 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 300
XapI RAATTY 2 cut(s) 104, 120
XspI CTAG 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.