Rh6AG352300

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000345
Physical Location & Seq
Forward (+)
55182301 .. 55191114
8814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG352300.1

Sequence Viewer

Length: 693 bp
ATGTCTGGTATTCGTATGACGATCCTGAATGGGGTGGCTCTTGCATTTGTTCTTTCCTTGTGTATCCCCATGTGCACCGGTTCATATGATCATTTTAAATTTGTACAACAATGGCCACCGGCCGTCTGTGATACGTCTGGCTGCAAGAGATCGGGGTTATCACACTTCACGATCCATGGCCTTTGGCCAAATAATAAAACGTATGTGAAAAATCAACCGACTTGTCTTACCAGTCATGCCAGCTCATTTAAGTCTACGATACTCACTAGTTCGAATTTGGTAAGCAAGCTGAGCACCTCTTGGCCCGATGTGATAAATGCAAATGATAAAGATTTTTGGAACAAACAATGGGACAAGCATGGCACATGTTCCTTGCAGACATACGACCAAGCACAATACTTCGAGCGGTCATACAACATGTGGAAAGAAACCAACCTGACAAACACCTTGGATAGCCTCATCAAACAGACACCGAGACAACAGAACGTCACAGATATAGAACAACTCATTCAAGGAGTAACTGCAACTGAAAAGAAACCCCTCCTCCGTTGTGAACAATCTCATAGTAATGCCAGTAAGTCTCTCTTGAAAGAGATTGTCATTTGCTATGCACACAACGGGATTACTGTGATCGATTGCGTCAGTCGACCAAAATCCACCTTCGAATGCTATGACGGATTTTGGTTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

230

Amino Acids

26.08

Weight (kDa)

8.49

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 29 - 213 4.7e-36 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 406
AccI GTMKAC 2 cut(s) 254, 646
AciI CCGC 1 cut(s) 406
AclWI GGATC 2 cut(s) 16, 166
AcoI YGGCCR 3 cut(s) 113, 120, 185
AcsI RAATTY 2 cut(s) 98, 274
AfaI GTAC 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 31
AflIII ACRYGT 2 cut(s) 365, 417
AgeI ACCGGT 1 cut(s) 77
AgsI TTSAA 2 cut(s) 512, 589
AhlI ACTAGT 1 cut(s) 266
AluBI AGCT 2 cut(s) 243, 289
AluI AGCT 2 cut(s) 243, 289
Alw21I GWGCWC 2 cut(s) 77, 296
Alw26I GTCTC 2 cut(s) 469, 585
Alw44I GTGCAC 1 cut(s) 73
AlwI GGATC 2 cut(s) 16, 166
AoxI GGCC 5 cut(s) 113, 120, 178, 185, 302
ApaLI GTGCAC 1 cut(s) 73
ApeKI GCWGC 1 cut(s) 141
ApoI RAATTY 2 cut(s) 98, 274
AsiGI ACCGGT 1 cut(s) 77
AspS9I GGNCC 1 cut(s) 303
AsuII TTCGAA 2 cut(s) 272, 663
BaeGI GKGCMC 1 cut(s) 77
BalI TGGCCA 2 cut(s) 115, 187
Bbv12I GWGCWC 2 cut(s) 77, 296
BbvI GCAGC 1 cut(s) 128
BceAI ACGGC 1 cut(s) 107
BciVI GTATCC 1 cut(s) 74
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 2 cut(s) 469, 585
BcuI ACTAGT 1 cut(s) 266
BfaI CTAG 1 cut(s) 267
BfuI GTATCC 1 cut(s) 74
BisI GCNGC 1 cut(s) 142
BlpI GCTNAGC 1 cut(s) 290
BlsI GCNGC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 303
Bpu1102I GCTNAGC 1 cut(s) 290
Bpu14I TTCGAA 2 cut(s) 272, 663
Bsa29I ATCGAT 1 cut(s) 633
BsaJI CCNNGG 2 cut(s) 175, 447
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 31
Bse118I RCCGGY 2 cut(s) 77, 118
Bse1I ACTGG 2 cut(s) 231, 573
BseCI ATCGAT 1 cut(s) 633
BseDI CCNNGG 2 cut(s) 175, 447
BseLI CCNNNNNNNGG 1 cut(s) 31
BseMII CTCAG 1 cut(s) 281
BseNI ACTGG 2 cut(s) 231, 573
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 1 cut(s) 77
BseX3I CGGCCG 1 cut(s) 120
BseXI GCAGC 1 cut(s) 128
Bsh1285I CGRYCG 1 cut(s) 123
BshFI GGCC 5 cut(s) 115, 122, 180, 187, 304
BshTI ACCGGT 1 cut(s) 77
BshVI ATCGAT 1 cut(s) 633
BsiEI CGRYCG 1 cut(s) 123
BsiHKAI GWGCWC 2 cut(s) 77, 296
BsiSI CCGG 2 cut(s) 78, 119
BslFI GGGAC 1 cut(s) 365
BslI CCNNNNNNNGG 1 cut(s) 31
BsmAI GTCTC 2 cut(s) 469, 585
BsmFI GGGAC 1 cut(s) 365
BsmI GAATGC 1 cut(s) 671
BsnI GGCC 5 cut(s) 115, 122, 180, 187, 304
Bsp119I TTCGAA 2 cut(s) 272, 663
Bsp1286I GDGCHC 2 cut(s) 77, 296
Bsp1407I TGTACA 1 cut(s) 103
Bsp143I GATC 5 cut(s) 21, 88, 149, 171, 630
Bsp1720I GCTNAGC 1 cut(s) 290
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 1 cut(s) 406
BspANI GGCC 5 cut(s) 115, 122, 180, 187, 304
BspCNI CTCAG 1 cut(s) 282
BspDI ATCGAT 1 cut(s) 633
BspPI GGATC 2 cut(s) 16, 166
BspT104I TTCGAA 2 cut(s) 272, 663
BsrBI CCGCTC 1 cut(s) 406
BsrFI RCCGGY 2 cut(s) 77, 118
BsrGI TGTACA 1 cut(s) 103
BsrI ACTGG 2 cut(s) 231, 573
BssAI RCCGGY 2 cut(s) 77, 118
BssECI CCNNGG 2 cut(s) 175, 447
BssMI GATC 5 cut(s) 21, 88, 149, 171, 630
BssT1I CCWWGG 2 cut(s) 175, 447
Bst4CI ACNGT 1 cut(s) 628
BstAUI TGTACA 1 cut(s) 103
BstBI TTCGAA 2 cut(s) 272, 663
BstC8I GCNNGC 2 cut(s) 241, 287
BstDEI CTNAG 1 cut(s) 290
BstDSI CCRYGG 1 cut(s) 175
BstKTI GATC 5 cut(s) 24, 91, 152, 174, 633
BstMAI GTCTC 2 cut(s) 469, 585
BstMBI GATC 5 cut(s) 21, 88, 149, 171, 630
BstMCI CGRYCG 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 291
BstNSI RCATGY 2 cut(s) 369, 421
BstSLI GKGCMC 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 128
BstZI CGGCCG 1 cut(s) 120
Bsu15I ATCGAT 1 cut(s) 633
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 5 cut(s) 115, 122, 180, 187, 304
BsuTUI ATCGAT 1 cut(s) 633
BtgI CCRYGG 1 cut(s) 175
Cac8I GCNNGC 2 cut(s) 241, 287
Cfr10I RCCGGY 2 cut(s) 77, 118
Cfr13I GGNCC 1 cut(s) 303
ClaI ATCGAT 1 cut(s) 633
CseI GACGC 1 cut(s) 628
Csp6I GTAC 1 cut(s) 104
CspAI ACCGGT 1 cut(s) 77
CviAII CATG 6 cut(s) 70, 176, 236, 359, 366, 418
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 1 cut(s) 290
DpnI GATC 5 cut(s) 23, 90, 151, 173, 632
DpnII GATC 5 cut(s) 21, 88, 149, 171, 630
DraI TTTAAA 1 cut(s) 97
EaeI YGGCCR 3 cut(s) 113, 120, 185
EagI CGGCCG 1 cut(s) 120
EclXI CGGCCG 1 cut(s) 120
Eco130I CCWWGG 2 cut(s) 175, 447
Eco52I CGGCCG 1 cut(s) 120
EcoT14I CCWWGG 2 cut(s) 175, 447
ErhI CCWWGG 2 cut(s) 175, 447
FaeI CATG 6 cut(s) 73, 179, 239, 362, 369, 421
FalI AAGNNNNNCTT 2 cut(s) 569, 601
FaqI GGGAC 1 cut(s) 365
FatI CATG 6 cut(s) 69, 175, 235, 358, 365, 417
FauNDI CATATG 1 cut(s) 85
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 2 cut(s) 254, 646
Fnu4HI GCNGC 1 cut(s) 142
Fsp4HI GCNGC 1 cut(s) 142
FspBI CTAG 1 cut(s) 267
GluI GCNGC 1 cut(s) 142
HaeIII GGCC 5 cut(s) 115, 122, 180, 187, 304
HapII CCGG 2 cut(s) 78, 119
HgaI GACGC 1 cut(s) 628
Hin1II CATG 6 cut(s) 73, 179, 239, 362, 369, 421
HincII GTYRAC 1 cut(s) 647
HindII GTYRAC 1 cut(s) 647
HpaII CCGG 2 cut(s) 78, 119
Hpy166II GTNNAC 4 cut(s) 75, 255, 554, 647
Hpy188III TCNNGA 3 cut(s) 25, 169, 586
Hpy8I GTNNAC 4 cut(s) 75, 255, 554, 647
HpyAV CCTTC 1 cut(s) 670
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4IV ACGT 3 cut(s) 134, 200, 486
HpyCH4V TGCA 7 cut(s) 44, 75, 144, 320, 376, 524, 611
HpyF10VI GCNNNNNNNGC 1 cut(s) 291
HpyF3I CTNAG 1 cut(s) 290
HpySE526I ACGT 3 cut(s) 134, 200, 486
Hsp92II CATG 6 cut(s) 73, 179, 239, 362, 369, 421
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 5 cut(s) 21, 88, 149, 171, 630
LpnPI CCDG 8 cut(s) 38, 91, 123, 132, 244, 253, 449, 586
Lsp1109I GCAGC 1 cut(s) 128
MaeI CTAG 1 cut(s) 267
MaeII ACGT 3 cut(s) 134, 200, 486
MaeIII GTNAC 2 cut(s) 487, 517
MalI GATC 5 cut(s) 23, 90, 151, 173, 632
MbiI CCGCTC 1 cut(s) 406
MboI GATC 5 cut(s) 21, 88, 149, 171, 630
MhlI GDGCHC 2 cut(s) 77, 296
MlsI TGGCCA 2 cut(s) 115, 187
MluCI AATT 2 cut(s) 98, 274
MluNI TGGCCA 2 cut(s) 115, 187
MnlI CCTC 4 cut(s) 307, 467, 551, 554
Mox20I TGGCCA 2 cut(s) 115, 187
MscI TGGCCA 2 cut(s) 115, 187
MseI TTAA 2 cut(s) 96, 249
MslI CAYNNNNRTG 1 cut(s) 567
Msp20I TGGCCA 2 cut(s) 115, 187
MspI CCGG 2 cut(s) 78, 119
Mva1269I GAATGC 1 cut(s) 671
MwoI GCNNNNNNNGC 1 cut(s) 291
NcoI CCATGG 1 cut(s) 175
NdeI CATATG 1 cut(s) 85
NdeII GATC 5 cut(s) 21, 88, 149, 171, 630
NlaIII CATG 6 cut(s) 73, 179, 239, 362, 369, 421
NmuCI GTSAC 1 cut(s) 487
NspI RCATGY 2 cut(s) 369, 421
NspV TTCGAA 2 cut(s) 272, 663
PciI ACATGT 2 cut(s) 365, 417
PctI GAATGC 1 cut(s) 671
PinAI ACCGGT 1 cut(s) 77
PkrI GCNGC 1 cut(s) 143
PscI ACATGT 2 cut(s) 365, 417
PspPI GGNCC 1 cut(s) 303
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
RseI CAYNNNNRTG 1 cut(s) 567
SalI GTCGAC 1 cut(s) 645
SaqAI TTAA 2 cut(s) 96, 249
SatI GCNGC 1 cut(s) 142
Sau3AI GATC 5 cut(s) 21, 88, 149, 171, 630
Sau96I GGNCC 1 cut(s) 303
SduI GDGCHC 2 cut(s) 77, 296
SetI ASST 9 cut(s) 137, 203, 245, 291, 299, 438, 449, 489, 662
SfuI TTCGAA 2 cut(s) 272, 663
SmiMI CAYNNNNRTG 1 cut(s) 567
SpeI ACTAGT 1 cut(s) 266
Sse9I AATT 2 cut(s) 98, 274
SsiI CCGC 1 cut(s) 406
SspMI CTAG 1 cut(s) 267
StyI CCWWGG 2 cut(s) 175, 447
TaaI ACNGT 1 cut(s) 628
TaiI ACGT 3 cut(s) 137, 203, 489
TaqI TCGA 5 cut(s) 272, 402, 633, 646, 663
TasI AATT 2 cut(s) 98, 274
TatI WGTACW 1 cut(s) 103
Tru1I TTAA 2 cut(s) 96, 249
Tru9I TTAA 2 cut(s) 96, 249
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 1 cut(s) 141
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 1 cut(s) 72
TspGWI ACGGA 2 cut(s) 536, 690
VneI GTGCAC 1 cut(s) 73
XapI RAATTY 2 cut(s) 98, 274
XceI RCATGY 2 cut(s) 369, 421
XmiI GTMKAC 2 cut(s) 254, 646
XspI CTAG 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.