Rh3DG333300

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000345
Physical Location & Seq
Forward (+)
37036575 .. 37062387
25813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG333300.1

Sequence Viewer

Length: 663 bp
ATGGCAATATTGAAATTGCCGTTCGCTTTCAATGTTCTTGCTTTCGCTTTTTATTTATGTTTCAGTGTGAGCACGGGGTATTACGAATATTTCAAACTTGTGCAACAATGGCCACCAACCACTTGCCAAAATGCAAACTGCCGCAGAGTACCGACACCTCGCCTCTTTACCCTCCATGGGTTTTGGCCATCTAATTATTCAAACAATGTCGTGGCTAATTGCACTAACGCAATATTTCAACGGATGGATCGCTCATTGGTATCTAAACTGAATATATCGTGGCCGAATGTGAAATATCCGAATAATATCCAGTTTTGGGATAAACAGTGGAGAAAACATGGCTCATGTTCCGTGCACACGTTTAACCAAACAGAGTACTTCACGCAAGCGGATAATCTTTGGAACTCACACAACATTACTGATATTCTTATAACTGGTGGGATCAAGCCAAATGGATCAGAATATGCATACGATACGGTAGGGCGGCCCATCCAAATTGCAACCGGGAAGGTACCCGAACTTCGTTGCGCACCATCCTCCCCTGGAAGGCAATTGTTGCATGAAGTAGTACTTTGTTATAATCATAAGGGAACAACGCCCATCGACTGTAACCCTTTACAGGCAACCTGCGATCGTAAGTCCCTAATAAAGTTTACTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

220

Amino Acids

25.21

Weight (kDa)

9.16

Isoelectric Point (pI)

39.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 203 9.9e-39 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 431, 579
Acc16I TGCGCA 1 cut(s) 529
Acc36I ACCTGC 1 cut(s) 635
Acc65I GGTACC 1 cut(s) 511
AccB1I GGYRCC 1 cut(s) 511
AciI CCGC 3 cut(s) 142, 389, 484
AclWI GGATC 3 cut(s) 255, 449, 463
AcoI YGGCCR 3 cut(s) 110, 185, 281
AfaI GTAC 4 cut(s) 150, 377, 513, 570
AfiI CCNNNNNNNGG 4 cut(s) 177, 316, 546, 619
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 5 cut(s) 13, 31, 94, 201, 239
AjnI CCWGG 1 cut(s) 541
Alw21I GWGCWC 2 cut(s) 74, 357
Alw44I GTGCAC 1 cut(s) 353
AlwI GGATC 3 cut(s) 255, 449, 463
AoxI GGCC 4 cut(s) 110, 185, 281, 485
ApaLI GTGCAC 1 cut(s) 353
Asp718I GGTACC 1 cut(s) 511
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 486
AsuC2I CCSGG 1 cut(s) 505
BaeGI GKGCMC 1 cut(s) 357
BalI TGGCCA 2 cut(s) 112, 187
BanI GGYRCC 1 cut(s) 511
BarI GAAGNNNNNNTAC 2 cut(s) 504, 536
Bbv12I GWGCWC 2 cut(s) 74, 357
BccI CCATC 5 cut(s) 196, 238, 497, 541, 608
BceAI ACGGC 1 cut(s) 4
BciT130I CCWGG 1 cut(s) 543
BcnI CCSGG 1 cut(s) 505
BfuAI ACCTGC 1 cut(s) 635
BisI GCNGC 2 cut(s) 142, 485
BlsI GCNGC 2 cut(s) 143, 486
BmcAI AGTACT 2 cut(s) 377, 570
Bme1390I CCNGG 2 cut(s) 505, 543
BmgT120I GGNCC 1 cut(s) 486
BmiI GGNNCC 1 cut(s) 513
BmrFI CCNGG 2 cut(s) 505, 543
BpuMI CCSGG 1 cut(s) 505
BsaJI CCNNGG 2 cut(s) 175, 541
Bsc4I CCNNNNNNNGG 4 cut(s) 177, 316, 546, 619
Bse1I ACTGG 2 cut(s) 310, 439
BseBI CCWGG 1 cut(s) 543
BseDI CCNNGG 2 cut(s) 175, 541
BseGI GGATG 3 cut(s) 249, 489, 533
BseLI CCNNNNNNNGG 4 cut(s) 177, 316, 546, 619
BseNI ACTGG 2 cut(s) 310, 439
BseSI GKGCMC 1 cut(s) 357
Bsh1285I CGRYCG 1 cut(s) 634
BshFI GGCC 4 cut(s) 112, 187, 283, 487
BshNI GGYRCC 1 cut(s) 511
BsiEI CGRYCG 1 cut(s) 634
BsiHKAI GWGCWC 2 cut(s) 74, 357
BsiSI CCGG 1 cut(s) 504
BslFI GGGAC 1 cut(s) 625
BslI CCNNNNNNNGG 4 cut(s) 177, 316, 546, 619
BsmFI GGGAC 1 cut(s) 625
BsnI GGCC 4 cut(s) 112, 187, 283, 487
Bsp1286I GDGCHC 2 cut(s) 74, 357
Bsp143I GATC 4 cut(s) 247, 441, 455, 631
Bsp19I CCATGG 1 cut(s) 175
BspACI CCGC 3 cut(s) 142, 389, 484
BspANI GGCC 4 cut(s) 112, 187, 283, 487
BspLI GGNNCC 1 cut(s) 513
BspMI ACCTGC 1 cut(s) 635
BspPI GGATC 3 cut(s) 255, 449, 463
BspT107I GGYRCC 1 cut(s) 511
BsrI ACTGG 2 cut(s) 310, 439
BssECI CCNNGG 2 cut(s) 175, 541
BssMI GATC 4 cut(s) 247, 441, 455, 631
BssT1I CCWWGG 1 cut(s) 175
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 3 cut(s) 327, 478, 608
BstAPI GCANNNNNTGC 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 1 cut(s) 657
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 3 cut(s) 249, 489, 533
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 4 cut(s) 250, 444, 458, 634
BstMBI GATC 4 cut(s) 247, 441, 455, 631
BstMCI CGRYCG 1 cut(s) 634
BstMWI GCNNNNNNNGC 2 cut(s) 109, 556
BstNI CCWGG 1 cut(s) 543
BstSCI CCNGG 2 cut(s) 503, 541
BstSLI GKGCMC 1 cut(s) 357
BsuRI GGCC 4 cut(s) 112, 187, 283, 487
BtgI CCRYGG 1 cut(s) 175
BtsCI GGATG 3 cut(s) 249, 489, 533
BtsIMutI CAGTG 2 cut(s) 70, 332
BveI ACCTGC 1 cut(s) 635
Cac8I GCNNGC 1 cut(s) 387
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 486
Csp6I GTAC 4 cut(s) 149, 376, 512, 569
CviAII CATG 4 cut(s) 176, 338, 345, 560
CviJI RGCY 7 cut(s) 112, 187, 215, 283, 342, 448, 487
CviKI_1 RGCY 7 cut(s) 112, 187, 215, 283, 342, 448, 487
CviQI GTAC 4 cut(s) 149, 376, 512, 569
DdeI CTNAG 1 cut(s) 657
DpnI GATC 4 cut(s) 249, 443, 457, 633
DpnII GATC 4 cut(s) 247, 441, 455, 631
EaeI YGGCCR 3 cut(s) 110, 185, 281
Eco130I CCWWGG 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 541
EcoT14I CCWWGG 1 cut(s) 175
EcoT22I ATGCAT 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 175
FaeI CATG 4 cut(s) 179, 341, 348, 563
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FaqI GGGAC 1 cut(s) 625
FatI CATG 4 cut(s) 175, 337, 344, 559
Fnu4HI GCNGC 2 cut(s) 142, 485
FokI GGATG 3 cut(s) 256, 476, 520
Fsp4HI GCNGC 2 cut(s) 142, 485
FspI TGCGCA 1 cut(s) 529
GlaI GCGC 1 cut(s) 529
GluI GCNGC 2 cut(s) 142, 485
HaeIII GGCC 4 cut(s) 112, 187, 283, 487
HapII CCGG 1 cut(s) 504
HhaI GCGC 1 cut(s) 530
Hin1II CATG 4 cut(s) 179, 341, 348, 563
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HpaII CCGG 1 cut(s) 504
Hpy166II GTNNAC 2 cut(s) 355, 654
Hpy188I TCNGA 2 cut(s) 300, 460
Hpy8I GTNNAC 2 cut(s) 355, 654
HpyAV CCTTC 2 cut(s) 502, 540
HpyCH4III ACNGT 3 cut(s) 327, 478, 608
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 7 cut(s) 103, 134, 222, 355, 467, 500, 559
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 556
HpyF3I CTNAG 1 cut(s) 657
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 4 cut(s) 179, 341, 348, 563
HspAI GCGC 1 cut(s) 528
KpnI GGTACC 1 cut(s) 515
Kzo9I GATC 4 cut(s) 247, 441, 455, 631
LpnPI CCDG 7 cut(s) 323, 420, 517, 528, 555, 605, 640
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 608
MalI GATC 4 cut(s) 249, 443, 457, 633
MboI GATC 4 cut(s) 247, 441, 455, 631
MfeI CAATTG 1 cut(s) 551
MhlI GDGCHC 2 cut(s) 74, 357
MlsI TGGCCA 2 cut(s) 112, 187
MluCI AATT 5 cut(s) 14, 193, 217, 495, 551
MluNI TGGCCA 2 cut(s) 112, 187
MnlI CCTC 4 cut(s) 168, 173, 182, 547
Mox20I TGGCCA 2 cut(s) 112, 187
Mph1103I ATGCAT 1 cut(s) 469
MscI TGGCCA 2 cut(s) 112, 187
MseI TTAA 1 cut(s) 363
Msp20I TGGCCA 2 cut(s) 112, 187
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 2 cut(s) 505, 543
MunI CAATTG 1 cut(s) 551
MvaI CCWGG 1 cut(s) 543
MwoI GCNNNNNNNGC 2 cut(s) 109, 556
NciI CCSGG 1 cut(s) 505
NcoI CCATGG 1 cut(s) 175
NdeII GATC 4 cut(s) 247, 441, 455, 631
NlaIII CATG 4 cut(s) 179, 341, 348, 563
NlaIV GGNNCC 1 cut(s) 513
NsbI TGCGCA 1 cut(s) 529
NsiI ATGCAT 1 cut(s) 469
PkrI GCNGC 2 cut(s) 143, 486
Ple19I CGATCG 1 cut(s) 634
PsiI TTATAA 2 cut(s) 431, 579
Psp6I CCWGG 1 cut(s) 541
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 1 cut(s) 513
PspPI GGNCC 1 cut(s) 486
PvuI CGATCG 1 cut(s) 634
RsaI GTAC 4 cut(s) 150, 377, 513, 570
RsaNI GTAC 4 cut(s) 149, 376, 512, 569
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 2 cut(s) 142, 485
Sau3AI GATC 4 cut(s) 247, 441, 455, 631
Sau96I GGNCC 1 cut(s) 486
ScaI AGTACT 2 cut(s) 377, 570
ScrFI CCNGG 2 cut(s) 505, 543
SduI GDGCHC 2 cut(s) 74, 357
SetI ASST 4 cut(s) 160, 362, 513, 629
Sse9I AATT 5 cut(s) 14, 193, 217, 495, 551
SsiI CCGC 3 cut(s) 142, 389, 484
SspI AATATT 3 cut(s) 9, 89, 234
StyD4I CCNGG 2 cut(s) 503, 541
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 3 cut(s) 327, 478, 608
TaiI ACGT 1 cut(s) 362
TaqI TCGA 1 cut(s) 603
TasI AATT 5 cut(s) 14, 193, 217, 495, 551
TatI WGTACW 2 cut(s) 375, 568
TauI GCSGC 2 cut(s) 144, 487
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 2 cut(s) 70, 332
TspDTI ATGAA 1 cut(s) 576
TspGWI ACGGA 2 cut(s) 256, 340
TspRI CASTG 2 cut(s) 70, 332
VneI GTGCAC 1 cut(s) 353
ZrmI AGTACT 2 cut(s) 377, 570
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.