MD10G1162800.v1.1

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000345
Physical Location & Seq
Reverse (-)
25352684 .. 25353451
768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1162800.v1.1.491

Sequence Viewer

Length: 678 bp
ATGCACTTCTTCAAGATTGTTCTCGTTGTCATCGCTCTTTGCTTCGTCACTTATCTAGGAAAGAGCAATGCTGCCACACCATATGATATCTTCCAATTCGTCCAGCAATCCCCAATCGTCTTCTGCCTCCGGAGTGGAATTTGTGGAAACCAACCAGTTCTGCGGCATACCTTTACGACACATGGCCTCTGGCTGAGCAACTTTACCGATCCATCTGCACCTATACTCTGCGCTGGCACCCTATTCGACAGAAATCAGATGAATGCTGATCATAATCTGCAATTACTTTTGTCCAATTCCTGGCCCAACTTCAACATTAGACGGACAAATATGGATTTCTGGGCGCATGAGTACAACAAGCACGGCAGGTGCTCGGACAATAAATTTTCCCAGACACAATACTTCCACGAAGCTTATAGATTATGGTTGACCTACAATGCACAATATCTATTTTCTCAAACAACTGGGATTGTACCAGGATATCAATATCATTACATCGACCTTGAATCGGCCGTTCAACGGACTATAGGAGGGAAAACACCTCTTCTTATGTGCAAGTATGACCGGCGGATCGCGTATCTGCTGGAAGTCGTCATCTGTTTTGACTACAACGCAGCGAATCCGGTCGATTGTGTCAGGACAACAAATTGTGGGGCATTGGTATCGTATTCACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

226

Amino Acids

25.92

Weight (kDa)

8.35

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 211 3.2e-31 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 357
Acc36I ACCTGC 1 cut(s) 357
AccB1I GGYRCC 1 cut(s) 236
AccB7I CCANNNNNTGG 1 cut(s) 300
AccII CGCG 1 cut(s) 575
AccIII TCCGGA 1 cut(s) 129
AciI CCGC 2 cut(s) 163, 568
AclWI GGATC 2 cut(s) 203, 578
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 2 cut(s) 138, 383
AfaI GTAC 2 cut(s) 353, 474
AfiI CCNNNNNNNGG 3 cut(s) 300, 508, 519
AgsI TTSAA 4 cut(s) 13, 313, 506, 518
AjnI CCWGG 2 cut(s) 299, 475
AluBI AGCT 1 cut(s) 413
AluI AGCT 1 cut(s) 413
Alw21I GWGCWC 1 cut(s) 374
AlwI GGATC 2 cut(s) 203, 578
Aor13HI TCCGGA 1 cut(s) 129
AoxI GGCC 3 cut(s) 184, 302, 510
ApeKI GCWGC 2 cut(s) 71, 614
ApoI RAATTY 2 cut(s) 138, 383
AspLEI GCGC 2 cut(s) 233, 346
AspS9I GGNCC 1 cut(s) 303
BanI GGYRCC 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 374
BbvI GCAGC 2 cut(s) 58, 626
BccI CCATC 1 cut(s) 220
BceAI ACGGC 2 cut(s) 379, 497
BcgI CGANNNNNNTGC 5 cut(s) 197, 226, 231, 260, 645
BciT130I CCWGG 2 cut(s) 301, 477
BclI TGATCA 1 cut(s) 268
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 525
BfuAI ACCTGC 1 cut(s) 357
BisI GCNGC 3 cut(s) 72, 164, 615
BlpI GCTNAGC 1 cut(s) 194
BlsI GCNGC 3 cut(s) 73, 165, 616
Bme1390I CCNGG 2 cut(s) 301, 477
BmgT120I GGNCC 1 cut(s) 303
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 2 cut(s) 301, 477
BmrI ACTGGG 1 cut(s) 474
BmuI ACTGGG 1 cut(s) 474
BpiI GAAGAC 1 cut(s) 112
Bpu1102I GCTNAGC 1 cut(s) 194
BsaBI GATNNNNATC 1 cut(s) 273
BsaWI WCCGGW 2 cut(s) 129, 622
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 508, 519
Bse118I RCCGGY 1 cut(s) 564
Bse1I ACTGG 2 cut(s) 155, 469
Bse3DI GCAATG 1 cut(s) 73
Bse8I GATNNNNATC 1 cut(s) 273
BseAI TCCGGA 1 cut(s) 129
BseBI CCWGG 2 cut(s) 301, 477
BseJI GATNNNNATC 1 cut(s) 273
BseLI CCNNNNNNNGG 3 cut(s) 300, 508, 519
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 2 cut(s) 155, 469
BseX3I CGGCCG 1 cut(s) 510
BseXI GCAGC 2 cut(s) 58, 626
BsgI GTGCAG 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 575
Bsh1285I CGRYCG 2 cut(s) 513, 627
BshFI GGCC 3 cut(s) 186, 304, 512
BshNI GGYRCC 1 cut(s) 236
BsiEI CGRYCG 2 cut(s) 513, 627
BsiHKAI GWGCWC 1 cut(s) 374
BsiSI CCGG 3 cut(s) 130, 565, 623
BslI CCNNNNNNNGG 3 cut(s) 300, 508, 519
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 3 cut(s) 186, 304, 512
Bsp1286I GDGCHC 1 cut(s) 374
Bsp13I TCCGGA 1 cut(s) 129
Bsp143I GATC 3 cut(s) 208, 268, 570
Bsp1720I GCTNAGC 1 cut(s) 194
BspACI CCGC 2 cut(s) 163, 568
BspANI GGCC 3 cut(s) 186, 304, 512
BspCNI CTCAG 1 cut(s) 186
BspEI TCCGGA 1 cut(s) 129
BspFNI CGCG 1 cut(s) 575
BspLI GGNNCC 1 cut(s) 238
BspMI ACCTGC 1 cut(s) 357
BspPI GGATC 2 cut(s) 203, 578
BspT107I GGYRCC 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 564
BsrI ACTGG 2 cut(s) 155, 469
BssAI RCCGGY 1 cut(s) 564
BssMI GATC 3 cut(s) 208, 268, 570
Bst2UI CCWGG 2 cut(s) 301, 477
Bst6I CTCTTC 1 cut(s) 549
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 1 cut(s) 194
BstFNI CGCG 1 cut(s) 575
BstHHI GCGC 2 cut(s) 233, 346
BstKTI GATC 3 cut(s) 211, 271, 573
BstMBI GATC 3 cut(s) 208, 268, 570
BstMCI CGRYCG 2 cut(s) 513, 627
BstNI CCWGG 2 cut(s) 301, 477
BstSCI CCNGG 2 cut(s) 299, 475
BstSFI CTRYAG 1 cut(s) 525
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 2 cut(s) 58, 626
BstV2I GAAGAC 1 cut(s) 112
BstZI CGGCCG 1 cut(s) 510
BsuRI GGCC 3 cut(s) 186, 304, 512
BtgZI GCGATG 1 cut(s) 16
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 235
CfoI GCGC 2 cut(s) 233, 346
Cfr10I RCCGGY 1 cut(s) 564
Cfr13I GGNCC 1 cut(s) 303
Csp6I GTAC 2 cut(s) 352, 473
CviAII CATG 2 cut(s) 182, 347
CviJI RGCY 5 cut(s) 186, 193, 304, 413, 512
CviKI_1 RGCY 5 cut(s) 186, 193, 304, 413, 512
CviQI GTAC 2 cut(s) 352, 473
DdeI CTNAG 1 cut(s) 194
DpnI GATC 3 cut(s) 210, 270, 572
DpnII GATC 3 cut(s) 208, 268, 570
EaeI YGGCCR 1 cut(s) 510
EagI CGGCCG 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 549
EarI CTCTTC 1 cut(s) 549
EciI GGCGGA 1 cut(s) 583
EclXI CGGCCG 1 cut(s) 510
Eco32I GATATC 2 cut(s) 88, 482
Eco52I CGGCCG 1 cut(s) 510
EcoRII CCWGG 2 cut(s) 299, 475
EcoRV GATATC 2 cut(s) 88, 482
FaeI CATG 2 cut(s) 185, 350
FatI CATG 2 cut(s) 181, 346
FauNDI CATATG 1 cut(s) 82
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 3 cut(s) 72, 164, 615
Fsp4HI GCNGC 3 cut(s) 72, 164, 615
FspBI CTAG 1 cut(s) 56
GlaI GCGC 2 cut(s) 232, 345
GluI GCNGC 3 cut(s) 72, 164, 615
HaeIII GGCC 3 cut(s) 186, 304, 512
HapII CCGG 3 cut(s) 130, 565, 623
HhaI GCGC 2 cut(s) 233, 346
Hin1II CATG 2 cut(s) 185, 350
Hin6I GCGC 2 cut(s) 231, 344
HinP1I GCGC 2 cut(s) 231, 344
HincII GTYRAC 1 cut(s) 429
HindII GTYRAC 1 cut(s) 429
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 2 cut(s) 506, 619
HpaII CCGG 3 cut(s) 130, 565, 623
Hpy166II GTNNAC 1 cut(s) 429
Hpy188I TCNGA 2 cut(s) 258, 376
Hpy188III TCNNGA 3 cut(s) 13, 130, 637
Hpy8I GTNNAC 1 cut(s) 429
HpyCH4V TGCA 5 cut(s) 4, 218, 280, 440, 555
HpyF3I CTNAG 1 cut(s) 194
Hsp92II CATG 2 cut(s) 185, 350
HspAI GCGC 2 cut(s) 231, 344
Kpn2I TCCGGA 1 cut(s) 129
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 3 cut(s) 208, 268, 570
Lsp1109I GCAGC 2 cut(s) 58, 626
MaeI CTAG 1 cut(s) 56
MaeIII GTNAC 1 cut(s) 46
MalI GATC 3 cut(s) 210, 270, 572
MboI GATC 3 cut(s) 208, 268, 570
MboII GAAGA 3 cut(s) 82, 112, 536
MhlI GDGCHC 1 cut(s) 374
MluCI AATT 6 cut(s) 95, 138, 281, 295, 383, 646
MnlI CCTC 4 cut(s) 137, 197, 524, 552
MroI TCCGGA 1 cut(s) 129
MspI CCGG 3 cut(s) 130, 565, 623
MspR9I CCNGG 2 cut(s) 301, 477
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 2 cut(s) 301, 477
MvnI CGCG 1 cut(s) 575
NdeI CATATG 1 cut(s) 82
NdeII GATC 3 cut(s) 208, 268, 570
NlaIII CATG 2 cut(s) 185, 350
NlaIV GGNNCC 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 46
PaqCI CACCTGC 1 cut(s) 357
PctI GAATGC 1 cut(s) 268
PfeI GAWTC 2 cut(s) 506, 619
PflMI CCANNNNNTGG 1 cut(s) 300
PkrI GCNGC 3 cut(s) 73, 165, 616
Psp6I CCWGG 2 cut(s) 299, 475
PspGI CCWGG 2 cut(s) 299, 475
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 1 cut(s) 303
RsaI GTAC 2 cut(s) 353, 474
RsaNI GTAC 2 cut(s) 352, 473
SatI GCNGC 3 cut(s) 72, 164, 615
Sau3AI GATC 3 cut(s) 208, 268, 570
Sau96I GGNCC 1 cut(s) 303
ScrFI CCNGG 2 cut(s) 301, 477
SduI GDGCHC 1 cut(s) 374
SetI ASST 7 cut(s) 173, 223, 371, 415, 434, 504, 544
SfcI CTRYAG 1 cut(s) 525
Sse9I AATT 6 cut(s) 95, 138, 281, 295, 383, 646
SsiI CCGC 2 cut(s) 163, 568
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 2 cut(s) 299, 475
TaqI TCGA 3 cut(s) 246, 498, 627
TasI AATT 6 cut(s) 95, 138, 281, 295, 383, 646
TatI WGTACW 1 cut(s) 351
TauI GCSGC 1 cut(s) 166
TfiI GAWTC 2 cut(s) 506, 619
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 2 cut(s) 71, 614
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 275
TspGWI ACGGA 2 cut(s) 337, 535
Van91I CCANNNNNTGG 1 cut(s) 300
XapI RAATTY 2 cut(s) 138, 383
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.