FvH4_6g07690

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000345
Physical Location & Seq
Reverse (-)
4634036 .. 4635081
1046 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07690.t1

Sequence Viewer

Length: 681 bp
ATGGGGATCATCCTACTTGTCCTGCTGCTTCTCTCTGCGTGTGGTGCAGATGATGCAGCGAACATCTACGACTACATGCAGTTCGTCCAGCAATGGCCGGCGACATTCTGCTATGGTAATCCGAATTGTGTCCCAAATCCACCACACAGTACTTTTACGATTCACGGCCTCTGGCCCAGCAATTACTCGACACCTGAATTTCCATGCGTTGGCACACCCTTTGATCCCTCGCAGATGTTCGCTGCCGAAAACCGTGGTTTGAGGGACTATTTTCTGCCGCAAGCATGGCCACAGTTGAATGCTCGTTATACCAACTTGGAGTTCTGGAAATTCGAGTACGAAAAGCACGGTATGTGCTCGGAGAACTATCTGAATCAGATGGATTATTTCAGGAAAGCTTACTGGGCGTGGGCTCGATTCAATGCTTATGTTCTGTTTGCGGCATCGCCTAAGCAAATCTACCCTGGCAATTACTATTACACAAGAGATATTGAAGCAGCCATTCAGAGAGTGACCAGTGAGAAGCCTCTGCTTATGTGCAAGAAGGTAAGAGTAGGTACGGTTGATAGTTGTCTATTGCAGGAGGTCATCATCTGTCTTGATCGTTGGGCAGACAACGTTGTACCTTGTCGCCTTCGACTGTCATCGTGCAACGTCCCAATTATATATTATGGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

227

Amino Acids

26.23

Weight (kDa)

6.28

Isoelectric Point (pI)

39.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 23 - 210 1.3e-34 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 209
AciI CCGC 2 cut(s) 278, 440
AclI AACGTT 1 cut(s) 618
AclWI GGATC 2 cut(s) 14, 218
AcoI YGGCCR 2 cut(s) 95, 287
AcsI RAATTY 2 cut(s) 197, 329
AfaI GTAC 4 cut(s) 151, 338, 559, 624
AfiI CCNNNNNNNGG 1 cut(s) 209
AgsI TTSAA 3 cut(s) 298, 421, 494
AjnI CCWGG 1 cut(s) 463
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 1 cut(s) 398
AluI AGCT 1 cut(s) 398
Alw21I GWGCWC 1 cut(s) 359
AlwI GGATC 2 cut(s) 14, 218
AoxI GGCC 4 cut(s) 95, 166, 173, 287
ApeKI GCWGC 4 cut(s) 25, 56, 242, 497
ApoI RAATTY 2 cut(s) 197, 329
AspS9I GGNCC 1 cut(s) 174
BalI TGGCCA 1 cut(s) 289
BanII GRGCYC 1 cut(s) 415
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 4 cut(s) 12, 68, 229, 509
BccI CCATC 1 cut(s) 373
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 1 cut(s) 465
BisI GCNGC 6 cut(s) 26, 57, 243, 278, 441, 498
BlsI GCNGC 6 cut(s) 27, 58, 244, 279, 442, 499
BmcAI AGTACT 1 cut(s) 151
Bme1390I CCNGG 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 465
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 2 cut(s) 43, 452
BmuI ACTGGG 1 cut(s) 412
Bpu10I CCTNAGC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 253, 463
Bsc4I CCNNNNNNNGG 1 cut(s) 209
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 2 cut(s) 407, 516
Bse3DI GCAATG 1 cut(s) 98
BseBI CCWGG 1 cut(s) 465
BseDI CCNNGG 2 cut(s) 253, 463
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 209
BseMI GCAATG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 407, 516
BseXI GCAGC 4 cut(s) 12, 68, 229, 509
BseYI CCCAGC 1 cut(s) 176
BsgI GTGCAG 1 cut(s) 66
BshFI GGCC 4 cut(s) 97, 168, 175, 289
BsiHKAI GWGCWC 1 cut(s) 359
BsiSI CCGG 1 cut(s) 98
BslFI GGGAC 3 cut(s) 116, 278, 641
BslI CCNNNNNNNGG 1 cut(s) 209
BsmFI GGGAC 3 cut(s) 116, 278, 641
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 4 cut(s) 97, 168, 175, 289
Bsp1286I GDGCHC 2 cut(s) 359, 415
Bsp143I GATC 3 cut(s) 6, 223, 601
BspACI CCGC 2 cut(s) 278, 440
BspANI GGCC 4 cut(s) 97, 168, 175, 289
BspPI GGATC 2 cut(s) 14, 218
BsrDI GCAATG 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 97
BsrI ACTGG 2 cut(s) 407, 516
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 2 cut(s) 253, 463
BssMI GATC 3 cut(s) 6, 223, 601
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 6 cut(s) 149, 254, 294, 350, 562, 642
BstAPI GCANNNNNTGC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 99, 282
BstDEI CTNAG 1 cut(s) 450
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 1 cut(s) 9
BstKTI GATC 3 cut(s) 9, 226, 604
BstMBI GATC 3 cut(s) 6, 223, 601
BstMWI GCNNNNNNNGC 4 cut(s) 44, 53, 286, 404
BstNI CCWGG 1 cut(s) 465
BstNSI RCATGY 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 463
BstV1I GCAGC 4 cut(s) 12, 68, 229, 509
BsuRI GGCC 4 cut(s) 97, 168, 175, 289
BtgI CCRYGG 1 cut(s) 253
BtgZI GCGATG 1 cut(s) 429
BtsCI GGATG 1 cut(s) 9
BtsIMutI CAGTG 1 cut(s) 523
Cac8I GCNNGC 2 cut(s) 99, 282
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 174
Csp6I GTAC 4 cut(s) 150, 337, 558, 623
CviAII CATG 3 cut(s) 76, 204, 285
CviJI RGCY 8 cut(s) 97, 168, 175, 289, 398, 413, 500, 526
CviKI_1 RGCY 8 cut(s) 97, 168, 175, 289, 398, 413, 500, 526
CviQI GTAC 4 cut(s) 150, 337, 558, 623
DdeI CTNAG 1 cut(s) 450
DpnI GATC 3 cut(s) 8, 225, 603
DpnII GATC 3 cut(s) 6, 223, 601
EaeI YGGCCR 2 cut(s) 95, 287
Eco24I GRGCYC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 463
EcoT38I GRGCYC 1 cut(s) 415
FaeI CATG 3 cut(s) 79, 207, 288
FaqI GGGAC 3 cut(s) 116, 278, 641
FatI CATG 3 cut(s) 75, 203, 284
Fnu4HI GCNGC 6 cut(s) 26, 57, 243, 278, 441, 498
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 6 cut(s) 26, 57, 243, 278, 441, 498
GluI GCNGC 6 cut(s) 26, 57, 243, 278, 441, 498
GsaI CCCAGC 1 cut(s) 180
HaeIII GGCC 4 cut(s) 97, 168, 175, 289
HapII CCGG 1 cut(s) 98
Hin1II CATG 3 cut(s) 79, 207, 288
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 3 cut(s) 160, 373, 417
HpaII CCGG 1 cut(s) 98
Hpy188I TCNGA 5 cut(s) 123, 361, 372, 378, 507
Hpy188III TCNNGA 3 cut(s) 325, 391, 599
HpyAV CCTTC 2 cut(s) 538, 644
HpyCH4III ACNGT 6 cut(s) 149, 254, 294, 350, 562, 642
HpyCH4IV ACGT 2 cut(s) 618, 654
HpyCH4V TGCA 6 cut(s) 47, 56, 79, 540, 580, 651
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 53, 286, 404
HpyF3I CTNAG 1 cut(s) 450
HpySE526I ACGT 2 cut(s) 618, 654
Hsp92II CATG 3 cut(s) 79, 207, 288
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
Kzo9I GATC 3 cut(s) 6, 223, 601
Lsp1109I GCAGC 4 cut(s) 12, 68, 229, 509
LweI GCATC 2 cut(s) 43, 452
MaeII ACGT 2 cut(s) 618, 654
MaeIII GTNAC 2 cut(s) 511, 674
MalI GATC 3 cut(s) 8, 225, 603
MboI GATC 3 cut(s) 6, 223, 601
MhlI GDGCHC 2 cut(s) 359, 415
MlsI TGGCCA 1 cut(s) 289
MluCI AATT 6 cut(s) 124, 181, 197, 329, 469, 660
MluNI TGGCCA 1 cut(s) 289
MnlI CCTC 5 cut(s) 179, 238, 255, 537, 577
Mox20I TGGCCA 1 cut(s) 289
MroNI GCCGGC 1 cut(s) 97
MscI TGGCCA 1 cut(s) 289
Msp20I TGGCCA 1 cut(s) 289
MspI CCGG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 465
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 1 cut(s) 465
MwoI GCNNNNNNNGC 4 cut(s) 44, 53, 286, 404
NaeI GCCGGC 1 cut(s) 99
NdeII GATC 3 cut(s) 6, 223, 601
NgoMIV GCCGGC 1 cut(s) 97
NlaIII CATG 3 cut(s) 79, 207, 288
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 79
PctI GAATGC 1 cut(s) 304
PdiI GCCGGC 1 cut(s) 99
PfeI GAWTC 3 cut(s) 160, 373, 417
PflMI CCANNNNNTGG 1 cut(s) 209
PkrI GCNGC 6 cut(s) 27, 58, 244, 279, 442, 499
Psp1406I AACGTT 1 cut(s) 618
Psp6I CCWGG 1 cut(s) 463
PspFI CCCAGC 1 cut(s) 176
PspGI CCWGG 1 cut(s) 463
PspPI GGNCC 1 cut(s) 174
RsaI GTAC 4 cut(s) 151, 338, 559, 624
RsaNI GTAC 4 cut(s) 150, 337, 558, 623
SatI GCNGC 6 cut(s) 26, 57, 243, 278, 441, 498
Sau3AI GATC 3 cut(s) 6, 223, 601
Sau96I GGNCC 1 cut(s) 174
ScaI AGTACT 1 cut(s) 151
ScrFI CCNGG 1 cut(s) 465
SduI GDGCHC 2 cut(s) 359, 415
SetI ASST 8 cut(s) 196, 400, 549, 559, 588, 621, 628, 657
SfaNI GCATC 2 cut(s) 43, 452
Sse9I AATT 6 cut(s) 124, 181, 197, 329, 469, 660
SsiI CCGC 2 cut(s) 278, 440
StyD4I CCNGG 1 cut(s) 463
TaaI ACNGT 6 cut(s) 149, 254, 294, 350, 562, 642
TaiI ACGT 2 cut(s) 621, 657
TaqI TCGA 4 cut(s) 188, 333, 415, 637
TasI AATT 6 cut(s) 124, 181, 197, 329, 469, 660
TatI WGTACW 1 cut(s) 149
TauI GCSGC 2 cut(s) 280, 443
TfiI GAWTC 3 cut(s) 160, 373, 417
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 4 cut(s) 25, 56, 242, 497
Tsp45I GTSAC 1 cut(s) 511
TspRI CASTG 1 cut(s) 523
Van91I CCANNNNNTGG 1 cut(s) 209
XapI RAATTY 2 cut(s) 197, 329
XceI RCATGY 1 cut(s) 79
ZrmI AGTACT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.