pycom15g14910

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000345
Physical Location & Seq
Reverse (-)
10292005 .. 10292801
797 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14910.1

Sequence Viewer

Length: 594 bp
ATGCAACCATCTCATCTTCAGTTCACTATTCATGGTCTCTGGCCTGCGAATAAATCATCCTCTTCATCCCTTACCTGCATGGGGGATGCCTTTAATGTGAATGGGATGGATGATGCACTGAAAAAGGAGCTACTCAGTTCGTGGTGGGATTGGAGAAAAGGGGACCATGTCGAATTCTGGCAAAGAGAGTATGATAAGCACGGAAAGTGCTCCGACAACGTGTTCCCGAAGACCGAGTACTTCCGGAAGACCCTAGCGACGTACCATGATTTTGATATAGCTCAAATCCTGCAGAAGGCTAATATTGTACCCCAACCACTTCAGCCAAAGATGTCGTTATACAAATTGTACTCCATCGATCAGATAACAAAGGCCATTGAGTCCGAAACTGGCGGACATTCCGTCAATATCAGATGCTATCAACAAATGCAGAAGCAGAGCAAGAAATTAATATATTTGGCCCAAGTCGCCCTCTGCTATGATGAATCTGGCAATAACAGAATTGACTGTGATCCAGGCGAAACAACCAGTTGCAAGTTTGACGACAAACTCCCGGAAACTGTACCAAGTATAGCCCACATAAAGCTATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

198

Amino Acids

22.71

Weight (kDa)

6.64

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 6 - 170 7.1e-32 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 243
AciI CCGC 1 cut(s) 393
AclWI GGATC 1 cut(s) 506
AcsI RAATTY 1 cut(s) 173
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 5 cut(s) 239, 263, 309, 350, 564
AfiI CCNNNNNNNGG 2 cut(s) 81, 295
AflIII ACRYGT 1 cut(s) 219
AhdI GACNNNNNGTC 1 cut(s) 401
AjnI CCWGG 1 cut(s) 514
AluBI AGCT 3 cut(s) 130, 281, 586
AluI AGCT 3 cut(s) 130, 281, 586
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 243
AoxI GGCC 3 cut(s) 41, 372, 459
ApoI RAATTY 1 cut(s) 173
AseI ATTAAT 1 cut(s) 449
AspS9I GGNCC 2 cut(s) 163, 460
AsuC2I CCSGG 1 cut(s) 554
AvaII GGWCC 1 cut(s) 163
BarI GAAGNNNNNNTAC 2 cut(s) 221, 253
BbsI GAAGAC 2 cut(s) 236, 254
Bbv12I GWGCWC 1 cut(s) 212
BccI CCATC 3 cut(s) 16, 100, 362
BciT130I CCWGG 1 cut(s) 516
BcnI CCSGG 1 cut(s) 554
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 1 cut(s) 254
BfmI CTRYAG 1 cut(s) 290
BfuAI ACCTGC 1 cut(s) 83
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 516, 554
Bme18I GGWCC 1 cut(s) 163
BmeRI GACNNNNNGTC 1 cut(s) 401
BmgT120I GGNCC 2 cut(s) 163, 460
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 2 cut(s) 516, 554
BmsI GCATC 3 cut(s) 76, 103, 404
BpiI GAAGAC 2 cut(s) 236, 254
BpuMI CCSGG 1 cut(s) 554
Bsa29I ATCGAT 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 41
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 295
Bse1I ACTGG 2 cut(s) 394, 528
BseAI TCCGGA 1 cut(s) 243
BseBI CCWGG 1 cut(s) 516
BseCI ATCGAT 1 cut(s) 357
BseGI GGATG 5 cut(s) 56, 65, 91, 111, 115
BseLI CCNNNNNNNGG 2 cut(s) 81, 295
BseMII CTCAG 1 cut(s) 148
BseNI ACTGG 2 cut(s) 394, 528
BshFI GGCC 3 cut(s) 43, 374, 461
BshVI ATCGAT 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 212
BsiSI CCGG 2 cut(s) 244, 554
BslFI GGGAC 1 cut(s) 176
BslI CCNNNNNNNGG 2 cut(s) 81, 295
BsmAI GTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 176
BsnI GGCC 3 cut(s) 43, 374, 461
Bso31I GGTCTC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 212
Bsp13I TCCGGA 1 cut(s) 243
Bsp143I GATC 2 cut(s) 358, 511
BspACI CCGC 1 cut(s) 393
BspANI GGCC 3 cut(s) 43, 374, 461
BspCNI CTCAG 1 cut(s) 147
BspDI ATCGAT 1 cut(s) 357
BspEI TCCGGA 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 164
BspMAI CTGCAG 1 cut(s) 294
BspMI ACCTGC 1 cut(s) 83
BspPI GGATC 1 cut(s) 506
BspTNI GGTCTC 1 cut(s) 41
BsrI ACTGG 2 cut(s) 394, 528
BssMI GATC 2 cut(s) 358, 511
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 2 cut(s) 509, 562
Bst6I CTCTTC 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 1 cut(s) 134
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 5 cut(s) 56, 65, 91, 111, 115
BstKTI GATC 2 cut(s) 361, 514
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 2 cut(s) 358, 511
BstMWI GCNNNNNNNGC 1 cut(s) 467
BstNI CCWGG 1 cut(s) 516
BstSCI CCNGG 2 cut(s) 514, 552
BstSFI CTRYAG 1 cut(s) 290
BstV2I GAAGAC 2 cut(s) 236, 254
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 3 cut(s) 43, 374, 461
BsuTUI ATCGAT 1 cut(s) 357
BtsCI GGATG 5 cut(s) 56, 65, 91, 111, 115
BtsIMutI CAGTG 1 cut(s) 116
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 1 cut(s) 45
Cfr13I GGNCC 2 cut(s) 163, 460
ClaI ATCGAT 1 cut(s) 357
Csp6I GTAC 5 cut(s) 238, 262, 308, 349, 563
CviAII CATG 4 cut(s) 32, 79, 167, 266
CviJI RGCY 9 cut(s) 43, 130, 281, 299, 325, 374, 461, 575, 586
CviKI_1 RGCY 9 cut(s) 43, 130, 281, 299, 325, 374, 461, 575, 586
CviQI GTAC 5 cut(s) 238, 262, 308, 349, 563
DdeI CTNAG 1 cut(s) 134
DpnI GATC 2 cut(s) 360, 513
DpnII GATC 2 cut(s) 358, 511
DriI GACNNNNNGTC 1 cut(s) 401
Eam1104I CTCTTC 1 cut(s) 67
Eam1105I GACNNNNNGTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 67
EciI GGCGGA 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 41
Eco47I GGWCC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 305
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 514
FaeI CATG 4 cut(s) 35, 82, 170, 269
FaqI GGGAC 1 cut(s) 176
FatI CATG 4 cut(s) 31, 78, 166, 265
FokI GGATG 5 cut(s) 43, 52, 98, 118, 122
FspBI CTAG 1 cut(s) 254
HaeIII GGCC 3 cut(s) 43, 374, 461
HapII CCGG 2 cut(s) 244, 554
Hin1II CATG 4 cut(s) 35, 82, 170, 269
HinfI GANTC 2 cut(s) 380, 485
HpaII CCGG 2 cut(s) 244, 554
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 4 cut(s) 214, 363, 385, 413
Hpy188III TCNNGA 2 cut(s) 226, 244
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 2 cut(s) 509, 562
HpyCH4IV ACGT 2 cut(s) 219, 260
HpyCH4V TGCA 6 cut(s) 4, 78, 116, 292, 430, 534
HpyF10VI GCNNNNNNNGC 1 cut(s) 467
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 2 cut(s) 219, 260
Hsp92II CATG 4 cut(s) 35, 82, 170, 269
Kpn2I TCCGGA 1 cut(s) 243
Kzo9I GATC 2 cut(s) 358, 511
LmnI GCTCC 2 cut(s) 127, 215
LweI GCATC 3 cut(s) 76, 103, 404
MaeI CTAG 1 cut(s) 254
MaeII ACGT 2 cut(s) 219, 260
MalI GATC 2 cut(s) 360, 513
MboI GATC 2 cut(s) 358, 511
MboII GAAGA 4 cut(s) 8, 54, 241, 259
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 4 cut(s) 173, 344, 446, 501
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 2 cut(s) 70, 482
MroI TCCGGA 1 cut(s) 243
MseI TTAA 2 cut(s) 93, 449
MspI CCGG 2 cut(s) 244, 554
MspR9I CCNGG 2 cut(s) 516, 554
MvaI CCWGG 1 cut(s) 516
MwoI GCNNNNNNNGC 1 cut(s) 467
NciI CCSGG 1 cut(s) 554
NdeII GATC 2 cut(s) 358, 511
NlaIII CATG 4 cut(s) 35, 82, 170, 269
NlaIV GGNNCC 1 cut(s) 164
PfeI GAWTC 1 cut(s) 485
PflFI GACNNNGTC 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 552
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PshBI ATTAAT 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 514
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 2 cut(s) 163, 460
PstI CTGCAG 1 cut(s) 294
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 5 cut(s) 239, 263, 309, 350, 564
RsaNI GTAC 5 cut(s) 238, 262, 308, 349, 563
SaqAI TTAA 2 cut(s) 93, 449
Sau3AI GATC 2 cut(s) 358, 511
Sau96I GGNCC 2 cut(s) 163, 460
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 516, 554
SduI GDGCHC 1 cut(s) 212
SetI ASST 6 cut(s) 77, 132, 222, 263, 283, 588
SfaNI GCATC 3 cut(s) 76, 103, 404
SfcI CTRYAG 1 cut(s) 290
SinI GGWCC 1 cut(s) 163
Sse9I AATT 4 cut(s) 173, 344, 446, 501
SsiI CCGC 1 cut(s) 393
SspI AATATT 1 cut(s) 304
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 2 cut(s) 514, 552
TaaI ACNGT 2 cut(s) 509, 562
TaiI ACGT 2 cut(s) 222, 263
TaqI TCGA 2 cut(s) 171, 357
TaqII GACCGA 1 cut(s) 248
TasI AATT 4 cut(s) 173, 344, 446, 501
TatI WGTACW 2 cut(s) 237, 348
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 2 cut(s) 93, 449
Tru9I TTAA 2 cut(s) 93, 449
TscAI CASTG 1 cut(s) 123
TspDTI ATGAA 3 cut(s) 20, 54, 498
TspGWI ACGGA 2 cut(s) 216, 391
TspRI CASTG 1 cut(s) 123
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 163
VspI ATTAAT 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 1 cut(s) 173
XspI CTAG 1 cut(s) 254
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.