pycom10g14020

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000345
Physical Location & Seq
Reverse (-)
17447410 .. 17448202
793 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g14020.2

Sequence Viewer

Length: 696 bp
ATGCACTTCTTCAAGATTGCTCTCGTTGTCCTCACTCTTTGCTTCATCACCTATCTCGGAAAGAGCAATGCTGCCACACCATATGACATCTTCCAGTTTGTCCAGCAATCCCCATTGGCCTTCTGCTACGGAACCAATATCTGCGGAGACCAACCAGTACTGCCCCGTACCTTTACGACGCACGGCCCCTGGCCAAGCAGCAGCACCAATCCAGCTGCTCCTTTAACCTGCGCTGGTACCGCATTCAGCCGTAGTGAGATGAATGATCCCAACAATCATTATCTGCAACCATTTTTGTCCTATTCCTGGCCCAACTTCAACATTAGACAAACAAATATGGATTTCTGGGAGTATGAGTACAACAAGCACAGCAGGTGCTCGGACAATACATTTTCCCAGACACAATACTTCCGTGAAGCTTATAGATTGTGGTCTACCTACAATGCAATCCATCTATTTTCTCAAACAACTTGGATTGTACCAGGCTATCCATATCGTTATATCGACCTTGAATTGGCCATTCGACGGACTATAGGAGGGAAATCACCTCTTCTTATGTGCAAGTATAACGGGGTGAGCCCGTATCTGGTGGAAGTCGTCATCTGCTTTGACTACAACGCAGCGAATCCGGTCGATTGTGTCAGGACAACAAATTGTGGAAATCCTAATGCATTGGTATGGTATTTACTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

232

Amino Acids

26.62

Weight (kDa)

7.53

Isoelectric Point (pI)

38.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 363
Acc36I ACCTGC 2 cut(s) 236, 363
Acc65I GGTACC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 236
AccI GTMKAC 1 cut(s) 434
AciI CCGC 2 cut(s) 144, 240
AclWI GGATC 1 cut(s) 260
AcoI YGGCCR 2 cut(s) 191, 516
AfaI GTAC 5 cut(s) 159, 169, 238, 359, 480
AfiI CCNNNNNNNGG 4 cut(s) 306, 514, 525, 586
AgsI TTSAA 3 cut(s) 13, 319, 512
AjnI CCWGG 3 cut(s) 188, 305, 481
AluBI AGCT 2 cut(s) 215, 419
AluI AGCT 2 cut(s) 215, 419
Alw21I GWGCWC 1 cut(s) 380
Alw26I GTCTC 1 cut(s) 141
AlwI GGATC 1 cut(s) 260
AoxI GGCC 5 cut(s) 117, 184, 191, 308, 516
ApeKI GCWGC 5 cut(s) 71, 198, 201, 215, 620
Asp718I GGTACC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 185, 309
AsuHPI GGTGA 3 cut(s) 40, 537, 586
BalI TGGCCA 2 cut(s) 193, 518
BanI GGYRCC 1 cut(s) 236
BanII GRGCYC 1 cut(s) 581
BarI GAAGNNNNNNTAC 1 cut(s) 674
Bbv12I GWGCWC 1 cut(s) 380
BbvI GCAGC 5 cut(s) 58, 202, 210, 213, 632
BccI CCATC 1 cut(s) 459
BceAI ACGGC 2 cut(s) 199, 234
BciT130I CCWGG 3 cut(s) 190, 307, 483
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 1 cut(s) 694
BfmI CTRYAG 1 cut(s) 531
BfuAI ACCTGC 2 cut(s) 236, 363
BisI GCNGC 5 cut(s) 72, 199, 202, 216, 621
BlsI GCNGC 5 cut(s) 73, 200, 203, 217, 622
BmcAI AGTACT 1 cut(s) 159
Bme1390I CCNGG 3 cut(s) 190, 307, 483
BmgT120I GGNCC 2 cut(s) 185, 309
BmiI GGNNCC 3 cut(s) 133, 187, 238
BmrFI CCNGG 3 cut(s) 190, 307, 483
BsaI GGTCTC 1 cut(s) 141
BsaJI CCNNGG 1 cut(s) 188
BsaWI WCCGGW 1 cut(s) 628
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 4 cut(s) 306, 514, 525, 586
Bse1I ACTGG 2 cut(s) 94, 155
Bse3DI GCAATG 1 cut(s) 73
BseBI CCWGG 3 cut(s) 190, 307, 483
BseDI CCNNGG 1 cut(s) 188
BseLI CCNNNNNNNGG 4 cut(s) 306, 514, 525, 586
BseMI GCAATG 1 cut(s) 73
BseNI ACTGG 2 cut(s) 94, 155
BseXI GCAGC 5 cut(s) 58, 202, 210, 213, 632
Bsh1285I CGRYCG 1 cut(s) 633
BshFI GGCC 5 cut(s) 119, 186, 193, 310, 518
BshNI GGYRCC 1 cut(s) 236
BsiEI CGRYCG 1 cut(s) 633
BsiHKAI GWGCWC 1 cut(s) 380
BsiSI CCGG 1 cut(s) 629
BslI CCNNNNNNNGG 4 cut(s) 306, 514, 525, 586
BsmAI GTCTC 1 cut(s) 141
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 5 cut(s) 119, 186, 193, 310, 518
Bso31I GGTCTC 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 380, 581
Bsp143I GATC 1 cut(s) 265
BspACI CCGC 2 cut(s) 144, 240
BspANI GGCC 5 cut(s) 119, 186, 193, 310, 518
BspLI GGNNCC 3 cut(s) 133, 187, 238
BspMI ACCTGC 2 cut(s) 236, 363
BspPI GGATC 1 cut(s) 260
BspT107I GGYRCC 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 2 cut(s) 94, 155
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 1 cut(s) 265
Bst2UI CCWGG 3 cut(s) 190, 307, 483
Bst6I CTCTTC 1 cut(s) 555
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 1 cut(s) 268
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 1 cut(s) 265
BstMCI CGRYCG 1 cut(s) 633
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 3 cut(s) 190, 307, 483
BstSCI CCNGG 3 cut(s) 188, 305, 481
BstSFI CTRYAG 1 cut(s) 531
BstV1I GCAGC 5 cut(s) 58, 202, 210, 213, 632
BsuRI GGCC 5 cut(s) 119, 186, 193, 310, 518
BveI ACCTGC 2 cut(s) 236, 363
CfoI GCGC 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 185, 309
CseI GACGC 1 cut(s) 187
Csp6I GTAC 5 cut(s) 158, 168, 237, 358, 479
CviQI GTAC 5 cut(s) 158, 168, 237, 358, 479
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
EaeI YGGCCR 2 cut(s) 191, 516
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 581
Eco31I GGTCTC 1 cut(s) 141
EcoRII CCWGG 3 cut(s) 188, 305, 481
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 581
FauNDI CATATG 1 cut(s) 82
FblI GTMKAC 1 cut(s) 434
Fnu4HI GCNGC 5 cut(s) 72, 199, 202, 216, 621
FriOI GRGCYC 1 cut(s) 581
Fsp4HI GCNGC 5 cut(s) 72, 199, 202, 216, 621
FspBI CTAG 1 cut(s) 694
GlaI GCGC 1 cut(s) 232
GluI GCNGC 5 cut(s) 72, 199, 202, 216, 621
HaeIII GGCC 5 cut(s) 119, 186, 193, 310, 518
HapII CCGG 1 cut(s) 629
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 233
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 1 cut(s) 625
HpaII CCGG 1 cut(s) 629
HphI GGTGA 3 cut(s) 40, 537, 586
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 2 cut(s) 59, 382
Hpy188III TCNNGA 2 cut(s) 13, 643
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 2 cut(s) 181, 528
HpyAV CCTTC 1 cut(s) 130
HpyCH4V TGCA 5 cut(s) 4, 286, 446, 561, 671
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HspAI GCGC 1 cut(s) 231
KpnI GGTACC 1 cut(s) 240
Kzo9I GATC 1 cut(s) 265
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 5 cut(s) 58, 202, 210, 213, 632
MaeI CTAG 1 cut(s) 694
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 3 cut(s) 82, 542, 682
MhlI GDGCHC 2 cut(s) 380, 581
MlsI TGGCCA 2 cut(s) 193, 518
MluCI AATT 2 cut(s) 512, 652
MluNI TGGCCA 2 cut(s) 193, 518
MnlI CCTC 3 cut(s) 41, 530, 558
Mox20I TGGCCA 2 cut(s) 193, 518
Mph1103I ATGCAT 1 cut(s) 673
MscI TGGCCA 2 cut(s) 193, 518
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 676
Msp20I TGGCCA 2 cut(s) 193, 518
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 1 cut(s) 629
MspR9I CCNGG 3 cut(s) 190, 307, 483
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 3 cut(s) 190, 307, 483
MwoI GCNNNNNNNGC 1 cut(s) 239
NdeI CATATG 1 cut(s) 82
NdeII GATC 1 cut(s) 265
NlaIV GGNNCC 3 cut(s) 133, 187, 238
NsiI ATGCAT 1 cut(s) 673
PaqCI CACCTGC 1 cut(s) 363
PctI GAATGC 1 cut(s) 242
PfeI GAWTC 1 cut(s) 625
PkrI GCNGC 5 cut(s) 73, 200, 203, 217, 622
Psp6I CCWGG 3 cut(s) 188, 305, 481
PspGI CCWGG 3 cut(s) 188, 305, 481
PspN4I GGNNCC 3 cut(s) 133, 187, 238
PspPI GGNCC 2 cut(s) 185, 309
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 5 cut(s) 159, 169, 238, 359, 480
RsaNI GTAC 5 cut(s) 158, 168, 237, 358, 479
RseI CAYNNNNRTG 1 cut(s) 676
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 5 cut(s) 72, 199, 202, 216, 621
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 2 cut(s) 185, 309
ScaI AGTACT 1 cut(s) 159
ScrFI CCNGG 3 cut(s) 190, 307, 483
SduI GDGCHC 2 cut(s) 380, 581
SetI ASST 9 cut(s) 53, 173, 217, 230, 377, 421, 440, 510, 550
SfcI CTRYAG 1 cut(s) 531
SmiMI CAYNNNNRTG 1 cut(s) 676
Sse9I AATT 2 cut(s) 512, 652
SsiI CCGC 2 cut(s) 144, 240
SspMI CTAG 1 cut(s) 694
StyD4I CCNGG 3 cut(s) 188, 305, 481
TaqI TCGA 3 cut(s) 504, 523, 633
TasI AATT 2 cut(s) 512, 652
TatI WGTACW 2 cut(s) 157, 357
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseI GCWGC 5 cut(s) 71, 198, 201, 215, 620
TspDTI ATGAA 2 cut(s) 34, 275
TspGWI ACGGA 3 cut(s) 144, 401, 541
XmiI GTMKAC 1 cut(s) 434
XspI CTAG 1 cut(s) 694
ZrmI AGTACT 1 cut(s) 159
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.