FvH4_6g07740

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000345
Physical Location & Seq
Forward (+)
4649328 .. 4650335
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07740.t1

Sequence Viewer

Length: 663 bp
ATGGAAATGGCATTGCTAGCGGTTTTTCTTATCTCAGCTGCTGCTTGTTCATCAGTCACTGAAGCAGCCAAGGCCTACGACTACTTCCAGTTCGTACAACAATATCCTATTACCTTATGCATGTTCGACACAAGTTGCATTCCAGGACAATCACTGCCTCGATACTTCTACATCCACGGCTTATGGCCCAGTAACTTCACTTATCCGCATGACGATTGTGTGGGTACACTGTTCGACTACAATCAGATGAGCAGTGATCCTACTCTCTCAAACAATTTGCTGCAATCATGGCCCAGCTTCACGTCCAAATCCCACATCAAATTCTGGGCGGATGAGTATCACAAGCACGGGACCTGTTCGGAGATAGACTACCCGCAGCACCGCTATTTTGCCGAAGCCCATATGCTCTGGACGTTCGTCAATGCATACCGTATCTTAGCAACATACAACATCGTTCCCGGCAGAACCTACCCTGCGGTTGACATTATATACTTCGTCCAAGCAGCACTCAAAGGAATCACACCATCTGTTATGTGCAAGGCCAATTCTGCGGGTCAAGAATATCTCCACGAGATCATCATATGTCTTGACAAAACGCTTACCAGATTCATCAATTGTGTTCGACCATCCACTTGCCGTACTACAGCGGTAATGTATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

221

Amino Acids

25.15

Weight (kDa)

6.21

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 26 - 206 5.4e-38 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 20, 206, 329, 374, 382, 476, 551, 647
AclWI GGATC 1 cut(s) 251
AcsI RAATTY 1 cut(s) 320
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 3 cut(s) 96, 226, 640
AjiI CACGTC 1 cut(s) 303
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 2 cut(s) 38, 297
AluI AGCT 2 cut(s) 38, 297
AlwI GGATC 1 cut(s) 251
AlwNI CAGNNNCTG 2 cut(s) 41, 59
AoxI GGCC 4 cut(s) 72, 185, 290, 540
ApeKI GCWGC 6 cut(s) 38, 41, 65, 280, 376, 503
ApoI RAATTY 1 cut(s) 320
AspS9I GGNCC 3 cut(s) 186, 291, 351
AsuC2I CCSGG 1 cut(s) 459
AsuNHI GCTAGC 1 cut(s) 16
AvaII GGWCC 1 cut(s) 351
BauI CACGAG 1 cut(s) 569
BbvI GCAGC 6 cut(s) 25, 28, 77, 267, 388, 515
BccI CCATC 2 cut(s) 532, 634
BceAI ACGGC 2 cut(s) 193, 621
BciT130I CCWGG 1 cut(s) 144
BcnI CCSGG 1 cut(s) 459
BfaI CTAG 2 cut(s) 17, 661
BfmI CTRYAG 1 cut(s) 642
BisI GCNGC 6 cut(s) 39, 42, 66, 281, 377, 504
BlsI GCNGC 6 cut(s) 40, 43, 67, 282, 378, 505
Bme1390I CCNGG 2 cut(s) 144, 459
Bme18I GGWCC 1 cut(s) 351
BmgBI CACGTC 1 cut(s) 303
BmgT120I GGNCC 3 cut(s) 186, 291, 351
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 2 cut(s) 144, 459
BmrI ACTGGG 1 cut(s) 183
BmtI GCTAGC 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 183
BoxI GACNNNNGTC 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 459
BsaBI GATNNNNATC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 69, 175
Bse1I ACTGG 2 cut(s) 88, 189
Bse3DI GCAATG 1 cut(s) 11
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 2 cut(s) 69, 175
BseGI GGATG 3 cut(s) 171, 337, 626
BseJI GATNNNNATC 1 cut(s) 336
BseMI GCAATG 1 cut(s) 11
BseMII CTCAG 1 cut(s) 48
BseNI ACTGG 2 cut(s) 88, 189
BseXI GCAGC 6 cut(s) 25, 28, 77, 267, 388, 515
BseYI CCCAGC 1 cut(s) 293
BshFI GGCC 4 cut(s) 74, 187, 292, 542
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 364
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 138
BsnI GGCC 4 cut(s) 74, 187, 292, 542
Bsp143I GATC 2 cut(s) 256, 573
BspACI CCGC 8 cut(s) 20, 206, 329, 374, 382, 476, 551, 647
BspANI GGCC 4 cut(s) 74, 187, 292, 542
BspCNI CTCAG 1 cut(s) 47
BspLI GGNNCC 1 cut(s) 352
BspOI GCTAGC 1 cut(s) 20
BspPI GGATC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 11
BsrI ACTGG 2 cut(s) 88, 189
BssECI CCNNGG 2 cut(s) 69, 175
BssMI GATC 2 cut(s) 256, 573
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 2 cut(s) 231, 431
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 2 cut(s) 34, 436
BstDSI CCRYGG 1 cut(s) 175
BstF5I GGATG 3 cut(s) 171, 337, 626
BstKTI GATC 2 cut(s) 259, 576
BstMBI GATC 2 cut(s) 256, 573
BstMWI GCNNNNNNNGC 4 cut(s) 17, 71, 289, 548
BstNI CCWGG 1 cut(s) 144
BstNSI RCATGY 1 cut(s) 124
BstPAI GACNNNNGTC 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 142, 457
BstSFI CTRYAG 1 cut(s) 642
BstV1I GCAGC 6 cut(s) 25, 28, 77, 267, 388, 515
BsuRI GGCC 4 cut(s) 74, 187, 292, 542
BtgI CCRYGG 1 cut(s) 175
BtrI CACGTC 1 cut(s) 303
BtsCI GGATG 3 cut(s) 171, 337, 626
BtsI GCAGTG 2 cut(s) 152, 259
BtsIMutI CAGTG 4 cut(s) 57, 152, 227, 259
Cac8I GCNNGC 1 cut(s) 18
CaiI CAGNNNCTG 2 cut(s) 41, 59
Cfr13I GGNCC 3 cut(s) 186, 291, 351
Csp6I GTAC 3 cut(s) 95, 225, 639
CviAII CATG 3 cut(s) 121, 209, 288
CviJI RGCY 9 cut(s) 38, 68, 74, 180, 187, 292, 297, 398, 542
CviKI_1 RGCY 9 cut(s) 38, 68, 74, 180, 187, 292, 297, 398, 542
CviQI GTAC 3 cut(s) 95, 225, 639
DdeI CTNAG 2 cut(s) 34, 436
DpnI GATC 2 cut(s) 258, 575
DpnII GATC 2 cut(s) 256, 573
EciI GGCGGA 1 cut(s) 344
Eco130I CCWWGG 1 cut(s) 69
Eco147I AGGCCT 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 351
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 69
EcoT22I ATGCAT 2 cut(s) 122, 427
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 3 cut(s) 124, 212, 291
FaqI GGGAC 1 cut(s) 364
FatI CATG 3 cut(s) 120, 208, 287
FauI CCCGC 2 cut(s) 381, 544
FauNDI CATATG 2 cut(s) 402, 581
Fnu4HI GCNGC 6 cut(s) 39, 42, 66, 281, 377, 504
FokI GGATG 3 cut(s) 158, 344, 613
Fsp4HI GCNGC 6 cut(s) 39, 42, 66, 281, 377, 504
FspBI CTAG 2 cut(s) 17, 661
GluI GCNGC 6 cut(s) 39, 42, 66, 281, 377, 504
GsaI CCCAGC 1 cut(s) 297
HaeIII GGCC 4 cut(s) 74, 187, 292, 542
HapII CCGG 1 cut(s) 459
Hin1II CATG 3 cut(s) 124, 212, 291
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HinfI GANTC 2 cut(s) 516, 606
HpaII CCGG 1 cut(s) 459
Hpy166II GTNNAC 2 cut(s) 227, 481
Hpy188I TCNGA 2 cut(s) 246, 361
Hpy188III TCNNGA 3 cut(s) 409, 557, 587
Hpy8I GTNNAC 2 cut(s) 227, 481
HpyCH4III ACNGT 2 cut(s) 231, 431
HpyCH4IV ACGT 2 cut(s) 302, 413
HpyCH4V TGCA 5 cut(s) 120, 138, 283, 425, 537
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 71, 289, 548
HpyF3I CTNAG 2 cut(s) 34, 436
HpySE526I ACGT 2 cut(s) 302, 413
Hsp92II CATG 3 cut(s) 124, 212, 291
Kzo9I GATC 2 cut(s) 256, 573
Lsp1109I GCAGC 6 cut(s) 25, 28, 77, 267, 388, 515
MaeI CTAG 2 cut(s) 17, 661
MaeII ACGT 2 cut(s) 302, 413
MaeIII GTNAC 2 cut(s) 55, 191
MalI GATC 2 cut(s) 258, 575
MboI GATC 2 cut(s) 256, 573
MfeI CAATTG 1 cut(s) 613
MluCI AATT 4 cut(s) 274, 320, 544, 613
MnlI CCTC 1 cut(s) 168
Mph1103I ATGCAT 2 cut(s) 122, 427
MspA1I CMGCKG 2 cut(s) 38, 647
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 2 cut(s) 144, 459
MunI CAATTG 1 cut(s) 613
Mva1269I GAATGC 1 cut(s) 138
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 4 cut(s) 17, 71, 289, 548
NciI CCSGG 1 cut(s) 459
NdeI CATATG 2 cut(s) 402, 581
NdeII GATC 2 cut(s) 256, 573
NheI GCTAGC 1 cut(s) 16
NlaIII CATG 3 cut(s) 124, 212, 291
NlaIV GGNNCC 1 cut(s) 352
NmuCI GTSAC 1 cut(s) 55
NsiI ATGCAT 2 cut(s) 122, 427
NspI RCATGY 1 cut(s) 124
PceI AGGCCT 1 cut(s) 74
PctI GAATGC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 516, 606
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 6 cut(s) 40, 43, 67, 282, 378, 505
PpuMI RGGWCCY 1 cut(s) 351
PshAI GACNNNNGTC 1 cut(s) 416
Psp5II RGGWCCY 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 142
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 3 cut(s) 186, 291, 351
PspPPI RGGWCCY 1 cut(s) 351
PstNI CAGNNNCTG 2 cut(s) 41, 59
PvuII CAGCTG 1 cut(s) 38
RsaI GTAC 3 cut(s) 96, 226, 640
RsaNI GTAC 3 cut(s) 95, 225, 639
SatI GCNGC 6 cut(s) 39, 42, 66, 281, 377, 504
Sau3AI GATC 2 cut(s) 256, 573
Sau96I GGNCC 3 cut(s) 186, 291, 351
ScrFI CCNGG 2 cut(s) 144, 459
SetI ASST 7 cut(s) 40, 116, 299, 305, 356, 416, 470
SfcI CTRYAG 1 cut(s) 642
SinI GGWCC 1 cut(s) 351
Sse9I AATT 4 cut(s) 274, 320, 544, 613
SseBI AGGCCT 1 cut(s) 74
SsiI CCGC 8 cut(s) 20, 206, 329, 374, 382, 476, 551, 647
SspMI CTAG 2 cut(s) 17, 661
StuI AGGCCT 1 cut(s) 74
StyD4I CCNGG 2 cut(s) 142, 457
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 231, 431
TaiI ACGT 2 cut(s) 305, 416
TaqI TCGA 4 cut(s) 126, 160, 234, 622
TasI AATT 4 cut(s) 274, 320, 544, 613
TfiI GAWTC 2 cut(s) 516, 606
TscAI CASTG 4 cut(s) 64, 159, 234, 259
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 6 cut(s) 38, 41, 65, 280, 376, 503
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 2 cut(s) 39, 598
TspRI CASTG 4 cut(s) 64, 159, 234, 259
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 1 cut(s) 320
XceI RCATGY 1 cut(s) 124
XspI CTAG 2 cut(s) 17, 661
Zsp2I ATGCAT 2 cut(s) 122, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.