Rh2AG227600

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000345
Physical Location & Seq
Reverse (-)
24150696 .. 24183883
33188 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG227600.1

Sequence Viewer

Length: 1308 bp
ATGAAGAATTCAAACTTCATTATCCTTCTATCAGTATATGCACTTGTACAAGTTCTCAACGTTTCAAATGCTGAACCCTACGAGTACATGCAATTTGTACTCCAGTACCCACCTGGAGTTTGCTATGACCAATCAAAAGGTCATTGCATCTCACCTCTCCCAACCAAGTTTCATGTACATGGAATATGGCCTTCTAACTTCTCCAATACACTTGTCCGTTGTGGGCACGCACTGAGGAATAACCCCTTTCATAAGGCGCAGATGACACCTCTGCAGACAGATTTGGGAAACTCATGGCCGTCCGTGTTAACACAAAACAACAATATGCAGTTCTGGGAGCATGAGTATATAGACCACGGTGCATGTGCAGTAGAGTCAGGTGTACCACCTTTCACTCAAAGGTCCTACTTTGAGAAAGGACACCAGCTATGGAACCAATATGACATCCTTGCAGTGCTTCGTCAAAGTGGTATTAAACCGGGTACTGCAACATCATACACTATGACTCAGCTTGTAACCGCAATCCAAAAGAAAATCGGGAGTAACAATACTCCTTTAATCATGTGCAGGAGGACAGCCCTTGGTTACACACTAAAGGAAGTGATAATCTGTCTGGATCACCAGGCAACAAATGTGGTCAGTTGCGCGCTCAATAATATTAGAAAAACAGATTGCCGAGATCCTTCGGGCAAGGTGACCTTTTCTCCACTCACGCACTTTCCATCCGGTCCTTCCATAGTGGACAGAGAATTGCATTCATATTCAATATGGGCCATCCCACCGAATGATGTGTCTTTTAGGATCAAGAAGTTGATGGAAGGTCTCAGGGCTGAGTTCGGTGGGCCGGAGATCAAACCACATATCACTGTTGTGGGGTCTATTCGTATGACGTATGAAGATGTGCTCAACAAATTCAGATCTCTTCCTTCTAAATATTTTCGTGCATACCAAGCAAAAGTTAATCTAGTGGTTACTAGCAATTTTTATCACCAATGTGTTTCCCTACTCATTGATTCATCAACGGAATTATCCAGTCGGTTAGACTGTACAACTCGCATATGCACTGCACATTGGGGTTTCCATAGGGGTGGTAGGCCATATTTGAGTCTCCTTTACAGAAATCTGACAGAAGAAGAGAGGAAAATAGCTCTAGAAAAAGTGTATGGATGGGGCCGAGGGGAACATGGTAGACTTGGTTTCGGCGATAATGATAAGAGCAGTAAAATGGTCCCGCAAAAGGTTCATCTTTTAGCTGGGGAGGATATTGTTCAGGTGAGAGATGAGTTTCTATGGAGAACTTACGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

435

Amino Acids

49.48

Weight (kDa)

8.91

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 215 3.6e-39 Ribonuclease T2 family
RCC1 PF00415 386 - 425 3.9e-08 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1189
AccII CGCG 1 cut(s) 647
AciI CCGC 2 cut(s) 519, 1232
AclI AACGTT 1 cut(s) 60
AclWI GGATC 3 cut(s) 624, 674, 809
AcoI YGGCCR 1 cut(s) 296
AcsI RAATTY 2 cut(s) 7, 911
AfaI GTAC 8 cut(s) 48, 86, 99, 107, 177, 384, 484, 1048
AfiI CCNNNNNNNGG 1 cut(s) 1237
AgsI TTSAA 3 cut(s) 12, 66, 765
AjnI CCWGG 2 cut(s) 112, 621
AleI CACNNNNGTG 2 cut(s) 869, 993
AluBI AGCT 4 cut(s) 427, 511, 1148, 1253
AluI AGCT 4 cut(s) 427, 511, 1148, 1253
Alw21I GWGCWC 1 cut(s) 906
Alw26I GTCTC 2 cut(s) 827, 1112
AlwI GGATC 3 cut(s) 624, 674, 809
AoxI GGCC 6 cut(s) 188, 296, 771, 842, 1094, 1171
ApoI RAATTY 2 cut(s) 7, 911
ArsI GACNNNNNNTTYG 2 cut(s) 776, 808
AspLEI GCGC 3 cut(s) 259, 647, 649
AspS9I GGNCC 6 cut(s) 402, 728, 771, 842, 1171, 1228
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 5 cut(s) 144, 611, 706, 980, 1285
AvaII GGWCC 3 cut(s) 402, 728, 1228
BaeGI GKGCMC 1 cut(s) 228
BaeI ACNNNNGTAYC 2 cut(s) 89, 122
Bbv12I GWGCWC 1 cut(s) 906
BccI CCATC 4 cut(s) 730, 782, 808, 1161
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 2 cut(s) 114, 623
BcnI CCSGG 1 cut(s) 480
BcoDI GTCTC 2 cut(s) 827, 1112
BfaI CTAG 3 cut(s) 965, 975, 1151
BfmI CTRYAG 1 cut(s) 272
BglII AGATCT 1 cut(s) 917
Bme1390I CCNGG 3 cut(s) 114, 480, 623
Bme18I GGWCC 3 cut(s) 402, 728, 1228
BmgT120I GGNCC 6 cut(s) 402, 728, 771, 842, 1171, 1228
BmiI GGNNCC 3 cut(s) 434, 1172, 1230
BmrFI CCNGG 3 cut(s) 114, 480, 623
BmsI GCATC 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 86, 135
BpuMI CCSGG 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 827
BsaJI CCNNGG 3 cut(s) 355, 580, 1174
BsaWI WCCGGW 1 cut(s) 725
BsaXI ACNNNNNCTCC 4 cut(s) 329, 359, 688, 718
Bsc4I CCNNNNNNNGG 1 cut(s) 1237
Bse1I ACTGG 2 cut(s) 103, 1032
Bse3DI GCAATG 1 cut(s) 142
BseBI CCWGG 2 cut(s) 114, 623
BseDI CCNNGG 3 cut(s) 355, 580, 1174
BseGI GGATG 4 cut(s) 444, 722, 774, 1172
BseLI CCNNNNNNNGG 1 cut(s) 1237
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 4 cut(s) 224, 521, 822, 838
BseNI ACTGG 2 cut(s) 103, 1032
BsePI GCGCGC 1 cut(s) 645
BseSI GKGCMC 1 cut(s) 228
BseYI CCCAGC 1 cut(s) 1253
BsgI GTGCAG 3 cut(s) 387, 586, 1050
Bsh1236I CGCG 1 cut(s) 647
BshFI GGCC 6 cut(s) 190, 298, 773, 844, 1096, 1173
BsiHKAI GWGCWC 1 cut(s) 906
BsiSI CCGG 3 cut(s) 479, 726, 845
BslFI GGGAC 1 cut(s) 1214
BslI CCNNNNNNNGG 1 cut(s) 1237
BsmAI GTCTC 2 cut(s) 827, 1112
BsmFI GGGAC 1 cut(s) 1214
BsmI GAATGC 1 cut(s) 754
BsnI GGCC 6 cut(s) 190, 298, 773, 844, 1096, 1173
Bso31I GGTCTC 1 cut(s) 827
Bsp1286I GDGCHC 2 cut(s) 228, 906
Bsp1407I TGTACA 3 cut(s) 46, 175, 1046
Bsp143I GATC 5 cut(s) 616, 679, 801, 849, 917
BspACI CCGC 2 cut(s) 519, 1232
BspANI GGCC 6 cut(s) 190, 298, 773, 844, 1096, 1173
BspCNI CTCAG 4 cut(s) 225, 520, 823, 837
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 3 cut(s) 434, 1172, 1230
BspMAI CTGCAG 1 cut(s) 276
BspPI GGATC 3 cut(s) 624, 674, 809
BspTNI GGTCTC 1 cut(s) 827
BsrDI GCAATG 1 cut(s) 142
BsrGI TGTACA 3 cut(s) 46, 175, 1046
BsrI ACTGG 2 cut(s) 103, 1032
BssECI CCNNGG 3 cut(s) 355, 580, 1174
BssHII GCGCGC 1 cut(s) 645
BssMI GATC 5 cut(s) 616, 679, 801, 849, 917
BssT1I CCWWGG 1 cut(s) 580
Bst2UI CCWGG 2 cut(s) 114, 623
Bst4CI ACNGT 3 cut(s) 359, 868, 1046
Bst6I CTCTTC 2 cut(s) 927, 1128
BstAUI TGTACA 3 cut(s) 46, 175, 1046
BstC8I GCNNGC 2 cut(s) 228, 647
BstDEI CTNAG 4 cut(s) 233, 507, 824, 831
BstDSI CCRYGG 1 cut(s) 355
BstEII GGTNACC 1 cut(s) 694
BstF5I GGATG 4 cut(s) 444, 722, 774, 1172
BstFNI CGCG 1 cut(s) 647
BstHHI GCGC 3 cut(s) 259, 647, 649
BstKTI GATC 5 cut(s) 619, 682, 804, 852, 920
BstMAI GTCTC 2 cut(s) 827, 1112
BstMBI GATC 5 cut(s) 616, 679, 801, 849, 917
BstMWI GCNNNNNNNGC 1 cut(s) 950
BstNI CCWGG 2 cut(s) 114, 623
BstNSI RCATGY 2 cut(s) 91, 366
BstPI GGTNACC 1 cut(s) 694
BstSCI CCNGG 3 cut(s) 112, 478, 621
BstSFI CTRYAG 1 cut(s) 272
BstSLI GKGCMC 1 cut(s) 228
BstUI CGCG 1 cut(s) 647
BstX2I RGATCY 2 cut(s) 679, 917
BstXI CCANNNNNNTGG 1 cut(s) 1088
BstYI RGATCY 2 cut(s) 679, 917
BsuRI GGCC 6 cut(s) 190, 298, 773, 844, 1096, 1173
BtgI CCRYGG 1 cut(s) 355
BtsCI GGATG 4 cut(s) 444, 722, 774, 1172
BtsI GCAGTG 2 cut(s) 459, 1062
BtsIMutI CAGTG 4 cut(s) 230, 459, 864, 1062
Cac8I GCNNGC 2 cut(s) 228, 647
CfoI GCGC 3 cut(s) 259, 647, 649
Cfr13I GGNCC 6 cut(s) 402, 728, 771, 842, 1171, 1228
Csp6I GTAC 8 cut(s) 47, 85, 98, 106, 176, 383, 483, 1047
CspCI CAANNNNNGTGG 2 cut(s) 615, 650
CviAII CATG 8 cut(s) 88, 173, 179, 294, 341, 363, 562, 1184
CviQI GTAC 8 cut(s) 47, 85, 98, 106, 176, 383, 483, 1047
DdeI CTNAG 4 cut(s) 233, 507, 824, 831
DpnI GATC 5 cut(s) 618, 681, 803, 851, 919
DpnII GATC 5 cut(s) 616, 679, 801, 849, 917
EaeI YGGCCR 1 cut(s) 296
Eam1104I CTCTTC 2 cut(s) 927, 1128
EarI CTCTTC 2 cut(s) 927, 1128
Eco130I CCWWGG 1 cut(s) 580
Eco31I GGTCTC 1 cut(s) 827
Eco47I GGWCC 3 cut(s) 402, 728, 1228
Eco91I GGTNACC 1 cut(s) 694
EcoO109I RGGNCCY 1 cut(s) 402
EcoO65I GGTNACC 1 cut(s) 694
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 112, 621
EcoT14I CCWWGG 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 580
FaeI CATG 8 cut(s) 91, 176, 182, 297, 344, 366, 565, 1187
FalI AAGNNNNNCTT 2 cut(s) 683, 715
FaqI GGGAC 1 cut(s) 1214
FatI CATG 8 cut(s) 87, 172, 178, 293, 340, 362, 561, 1183
FauI CCCGC 1 cut(s) 1239
FauNDI CATATG 1 cut(s) 1058
FblI GTMKAC 1 cut(s) 1189
FokI GGATG 4 cut(s) 431, 709, 761, 1179
FspBI CTAG 3 cut(s) 965, 975, 1151
GlaI GCGC 3 cut(s) 258, 646, 648
GsaI CCCAGC 1 cut(s) 1257
GsuI CTGGAG 2 cut(s) 86, 135
HaeIII GGCC 6 cut(s) 190, 298, 773, 844, 1096, 1173
HapII CCGG 3 cut(s) 479, 726, 845
HhaI GCGC 3 cut(s) 259, 647, 649
Hin1II CATG 8 cut(s) 91, 176, 182, 297, 344, 366, 565, 1187
Hin6I GCGC 3 cut(s) 257, 645, 647
HinP1I GCGC 3 cut(s) 257, 645, 647
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 4 cut(s) 374, 505, 1013, 1105
HpaI GTTAAC 1 cut(s) 309
HpaII CCGG 3 cut(s) 479, 726, 845
HphI GGTGA 5 cut(s) 144, 611, 706, 980, 1285
Hpy166II GTNNAC 4 cut(s) 309, 383, 742, 1190
Hpy188I TCNGA 2 cut(s) 917, 1125
Hpy188III TCNNGA 4 cut(s) 538, 614, 805, 1151
Hpy8I GTNNAC 4 cut(s) 309, 383, 742, 1190
HpyAV CCTTC 6 cut(s) 35, 201, 693, 741, 812, 936
HpyCH4III ACNGT 3 cut(s) 359, 868, 1046
HpyCH4IV ACGT 2 cut(s) 60, 890
HpyF10VI GCNNNNNNNGC 1 cut(s) 950
HpyF3I CTNAG 4 cut(s) 233, 507, 824, 831
HpySE526I ACGT 2 cut(s) 60, 890
Hsp92II CATG 8 cut(s) 91, 176, 182, 297, 344, 366, 565, 1187
HspAI GCGC 3 cut(s) 257, 645, 647
KspAI GTTAAC 1 cut(s) 309
Kzo9I GATC 5 cut(s) 616, 679, 801, 849, 917
LmnI GCTCC 1 cut(s) 337
LweI GCATC 1 cut(s) 156
MaeI CTAG 3 cut(s) 965, 975, 1151
MaeII ACGT 2 cut(s) 60, 890
MaeIII GTNAC 5 cut(s) 514, 542, 584, 694, 970
MalI GATC 5 cut(s) 618, 681, 803, 851, 919
MboI GATC 5 cut(s) 616, 679, 801, 849, 917
MboII GAAGA 5 cut(s) 16, 908, 914, 1142, 1145
MflI RGATCY 2 cut(s) 679, 917
MhlI GDGCHC 2 cut(s) 228, 906
MluCI AATT 6 cut(s) 7, 92, 749, 911, 979, 1025
MlyI GAGTC 3 cut(s) 383, 499, 1114
MnlI CCTC 7 cut(s) 165, 228, 279, 564, 1131, 1169, 1252
MseI TTAA 4 cut(s) 308, 474, 557, 960
MslI CAYNNNNRTG 4 cut(s) 177, 869, 993, 1086
MspI CCGG 3 cut(s) 479, 726, 845
MspR9I CCNGG 3 cut(s) 114, 480, 623
Mva1269I GAATGC 1 cut(s) 754
MvaI CCWGG 2 cut(s) 114, 623
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 1 cut(s) 950
NciI CCSGG 1 cut(s) 480
NdeI CATATG 1 cut(s) 1058
NdeII GATC 5 cut(s) 616, 679, 801, 849, 917
NlaIII CATG 8 cut(s) 91, 176, 182, 297, 344, 366, 565, 1187
NlaIV GGNNCC 3 cut(s) 434, 1172, 1230
NmeAIII GCCGAG 2 cut(s) 701, 1199
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 2 cut(s) 91, 366
OliI CACNNNNGTG 2 cut(s) 869, 993
PauI GCGCGC 1 cut(s) 645
PctI GAATGC 1 cut(s) 754
PfeI GAWTC 1 cut(s) 1013
PleI GAGTC 3 cut(s) 382, 499, 1113
PpsI GAGTC 3 cut(s) 382, 499, 1113
PpuMI RGGWCCY 1 cut(s) 402
Psp1406I AACGTT 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 112, 621
PspEI GGTNACC 1 cut(s) 694
PspFI CCCAGC 1 cut(s) 1253
PspGI CCWGG 2 cut(s) 112, 621
PspN4I GGNNCC 3 cut(s) 434, 1172, 1230
PspPI GGNCC 6 cut(s) 402, 728, 771, 842, 1171, 1228
PspPPI RGGWCCY 1 cut(s) 402
PstI CTGCAG 1 cut(s) 276
PsuI RGATCY 2 cut(s) 679, 917
PteI GCGCGC 1 cut(s) 645
RsaI GTAC 8 cut(s) 48, 86, 99, 107, 177, 384, 484, 1048
RsaNI GTAC 8 cut(s) 47, 85, 98, 106, 176, 383, 483, 1047
RseI CAYNNNNRTG 4 cut(s) 177, 869, 993, 1086
SaqAI TTAA 4 cut(s) 308, 474, 557, 960
Sau3AI GATC 5 cut(s) 616, 679, 801, 849, 917
Sau96I GGNCC 6 cut(s) 402, 728, 771, 842, 1171, 1228
SchI GAGTC 3 cut(s) 383, 499, 1114
ScrFI CCNGG 3 cut(s) 114, 480, 623
SduI GDGCHC 2 cut(s) 228, 906
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 1 cut(s) 272
SinI GGWCC 3 cut(s) 402, 728, 1228
SmiMI CAYNNNNRTG 4 cut(s) 177, 869, 993, 1086
Sse9I AATT 6 cut(s) 7, 92, 749, 911, 979, 1025
SsiI CCGC 2 cut(s) 519, 1232
SspI AATATT 2 cut(s) 658, 935
SspMI CTAG 3 cut(s) 965, 975, 1151
StyD4I CCNGG 3 cut(s) 112, 478, 621
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 3 cut(s) 359, 868, 1046
TaiI ACGT 2 cut(s) 63, 893
TasI AATT 6 cut(s) 7, 92, 749, 911, 979, 1025
TatI WGTACW 5 cut(s) 46, 84, 97, 175, 1046
TfiI GAWTC 1 cut(s) 1013
Tru1I TTAA 4 cut(s) 308, 474, 557, 960
Tru9I TTAA 4 cut(s) 308, 474, 557, 960
TscAI CASTG 4 cut(s) 237, 459, 871, 1069
TseFI GTSAC 1 cut(s) 694
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 8 cut(s) 7, 17, 161, 239, 747, 909, 1005, 1232
TspGWI ACGGA 3 cut(s) 206, 292, 1037
TspRI CASTG 4 cut(s) 237, 459, 871, 1069
VpaK11BI GGWCC 3 cut(s) 402, 728, 1228
XapI RAATTY 2 cut(s) 7, 911
XbaI TCTAGA 1 cut(s) 1150
XceI RCATGY 2 cut(s) 91, 366
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 1189
XspI CTAG 3 cut(s) 965, 975, 1151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.