MD15G1167600.v1.1

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000345
Physical Location & Seq
Reverse (-)
12791832 .. 12792604
773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1167600.v1.1.491

Sequence Viewer

Length: 606 bp
ATGCAACCATCTCATCTTCAGTTCACTATTCATGGTCTCTGGCCTGCGAATAAATCATCCTCTTCATCCCTTGACTGCAAGGGGGATGCCTTTAATGTGAATGAGATGGATGATGCACTGAAAAAGGCGCTACTTAGTTCGTGGTGGGATTGGAGAAAAGGGAAGCCTGTCGAATTCTGGCAACGAGAGTATGATAAGCACGGAAAGTGCTCCGACAATGTGTTCCTGAAGACCGAGTACTTCCGGAAGACCCTAGCGATGTACCATGATTTTGATATAGCTCAAATCTTGCAGAAGGATAATATTGTACCCCAACCACTTCAGCCCGAGATGTCGTTGTACAAATTGTACTCCATCGATCAGATAACAAAGGCAATTGAGTCCGAAACCGGCGGACATTCCGTCAATATCAGATGCTATCAACAATACCAGAAGAAGAGCAACAATGGAAGTAAACCATTAATATATTTGGCCCAAGTCGCCCTCTGCTATAATAGATCTGGCAATAACAGAACTAACTGTGATCCAGGCGAAACAACCAGCTGCAACTTTAATAAATCAGAACCGAAAACTGTACCACGTATAGCCCACATAAAGCTCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

202

Amino Acids

23.23

Weight (kDa)

8.81

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 6 - 174 2.8e-30 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 243
AciI CCGC 1 cut(s) 393
AclWI GGATC 1 cut(s) 518
AcsI RAATTY 1 cut(s) 173
AcuI CTGAAG 2 cut(s) 248, 305
AfaI GTAC 6 cut(s) 239, 263, 309, 341, 350, 576
AhdI GACNNNNNGTC 1 cut(s) 401
AjnI CCWGG 1 cut(s) 526
AluBI AGCT 3 cut(s) 281, 543, 598
AluI AGCT 3 cut(s) 281, 543, 598
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 326
Aor13HI TCCGGA 1 cut(s) 243
AoxI GGCC 2 cut(s) 41, 471
ApeKI GCWGC 1 cut(s) 543
ApoI RAATTY 1 cut(s) 173
AseI ATTAAT 1 cut(s) 461
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 472
AvaI CYCGRG 1 cut(s) 326
BarI GAAGNNNNNNTAC 2 cut(s) 221, 253
BbsI GAAGAC 2 cut(s) 236, 254
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 1 cut(s) 530
BccI CCATC 3 cut(s) 16, 100, 362
BciT130I CCWGG 1 cut(s) 528
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 1 cut(s) 254
BfoI RGCGCY 1 cut(s) 131
BglII AGATCT 1 cut(s) 497
BisI GCNGC 1 cut(s) 544
BlsI GCNGC 1 cut(s) 545
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 1 cut(s) 528
BmeRI GACNNNNNGTC 1 cut(s) 401
BmeT110I CYCGRG 1 cut(s) 326
BmgT120I GGNCC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 528
BmsI GCATC 3 cut(s) 76, 103, 404
BpiI GAAGAC 2 cut(s) 236, 254
Bsa29I ATCGAT 1 cut(s) 357
BsaAI YACGTR 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 41
BsaWI WCCGGW 1 cut(s) 243
Bse118I RCCGGY 1 cut(s) 389
BseAI TCCGGA 1 cut(s) 243
BseBI CCWGG 1 cut(s) 528
BseCI ATCGAT 1 cut(s) 357
BseGI GGATG 4 cut(s) 56, 65, 91, 115
BseXI GCAGC 1 cut(s) 530
BshFI GGCC 2 cut(s) 43, 473
BshVI ATCGAT 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 326
BsiSI CCGG 2 cut(s) 244, 390
BsmAI GTCTC 1 cut(s) 41
BsnI GGCC 2 cut(s) 43, 473
Bso31I GGTCTC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 326
Bsp1286I GDGCHC 1 cut(s) 212
Bsp13I TCCGGA 1 cut(s) 243
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 3 cut(s) 358, 497, 523
BspACI CCGC 1 cut(s) 393
BspANI GGCC 2 cut(s) 43, 473
BspDI ATCGAT 1 cut(s) 357
BspEI TCCGGA 1 cut(s) 243
BspPI GGATC 1 cut(s) 518
BspQI GCTCTTC 1 cut(s) 431
BspTNI GGTCTC 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 389
BsrGI TGTACA 1 cut(s) 339
BssAI RCCGGY 1 cut(s) 389
BssMI GATC 3 cut(s) 358, 497, 523
Bst2UI CCWGG 1 cut(s) 528
Bst4CI ACNGT 2 cut(s) 521, 574
Bst6I CTCTTC 2 cut(s) 67, 431
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 581
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 1 cut(s) 134
BstF5I GGATG 4 cut(s) 56, 65, 91, 115
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 3 cut(s) 361, 500, 526
BstMAI GTCTC 1 cut(s) 41
BstMBI GATC 3 cut(s) 358, 497, 523
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 528
BstSCI CCNGG 1 cut(s) 526
BstV1I GCAGC 1 cut(s) 530
BstV2I GAAGAC 2 cut(s) 236, 254
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 2 cut(s) 43, 473
BsuTUI ATCGAT 1 cut(s) 357
BtgZI GCGATG 1 cut(s) 272
BtsCI GGATG 4 cut(s) 56, 65, 91, 115
BtsIMutI CAGTG 1 cut(s) 116
Cac8I GCNNGC 1 cut(s) 45
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 1 cut(s) 472
ClaI ATCGAT 1 cut(s) 357
Csp6I GTAC 6 cut(s) 238, 262, 308, 340, 349, 575
CviAII CATG 2 cut(s) 32, 266
CviJI RGCY 8 cut(s) 43, 166, 281, 325, 473, 543, 587, 598
CviKI_1 RGCY 8 cut(s) 43, 166, 281, 325, 473, 543, 587, 598
CviQI GTAC 6 cut(s) 238, 262, 308, 340, 349, 575
DdeI CTNAG 1 cut(s) 134
DpnI GATC 3 cut(s) 360, 499, 525
DpnII GATC 3 cut(s) 358, 497, 523
DriI GACNNNNNGTC 1 cut(s) 401
Eam1104I CTCTTC 2 cut(s) 67, 431
Eam1105I GACNNNNNGTC 1 cut(s) 401
EarI CTCTTC 2 cut(s) 67, 431
EciI GGCGGA 1 cut(s) 408
Eco31I GGTCTC 1 cut(s) 41
Eco57I CTGAAG 2 cut(s) 248, 305
Eco88I CYCGRG 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 526
FaeI CATG 2 cut(s) 35, 269
FaiI YATR 8 cut(s) 33, 192, 267, 278, 466, 492, 584, 593
FatI CATG 2 cut(s) 31, 265
Fnu4HI GCNGC 1 cut(s) 544
FokI GGATG 4 cut(s) 43, 52, 98, 122
Fsp4HI GCNGC 1 cut(s) 544
FspBI CTAG 1 cut(s) 254
GlaI GCGC 1 cut(s) 129
GluI GCNGC 1 cut(s) 544
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 43, 473
HapII CCGG 2 cut(s) 244, 390
HhaI GCGC 1 cut(s) 130
Hin1II CATG 2 cut(s) 35, 269
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 1 cut(s) 380
HpaII CCGG 2 cut(s) 244, 390
Hpy166II GTNNAC 2 cut(s) 24, 455
Hpy188I TCNGA 5 cut(s) 214, 363, 385, 413, 562
Hpy188III TCNNGA 2 cut(s) 226, 244
Hpy8I GTNNAC 2 cut(s) 24, 455
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 2 cut(s) 521, 574
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 5 cut(s) 4, 78, 116, 292, 546
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 580
Hsp92II CATG 2 cut(s) 35, 269
HspAI GCGC 1 cut(s) 128
Kpn2I TCCGGA 1 cut(s) 243
Kzo9I GATC 3 cut(s) 358, 497, 523
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 1 cut(s) 215
Lsp1109I GCAGC 1 cut(s) 530
LweI GCATC 3 cut(s) 76, 103, 404
MaeI CTAG 1 cut(s) 254
MaeII ACGT 1 cut(s) 580
MalI GATC 3 cut(s) 360, 499, 525
MboI GATC 3 cut(s) 358, 497, 523
MboII GAAGA 6 cut(s) 8, 54, 241, 259, 445, 448
MfeI CAATTG 1 cut(s) 375
MflI RGATCY 1 cut(s) 497
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 3 cut(s) 173, 344, 375
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 2 cut(s) 70, 494
MroI TCCGGA 1 cut(s) 243
MseI TTAA 3 cut(s) 93, 461, 552
MspA1I CMGCKG 1 cut(s) 543
MspI CCGG 2 cut(s) 244, 390
MspR9I CCNGG 1 cut(s) 528
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 1 cut(s) 528
MwoI GCNNNNNNNGC 1 cut(s) 479
NdeII GATC 3 cut(s) 358, 497, 523
NlaIII CATG 2 cut(s) 35, 269
PciSI GCTCTTC 1 cut(s) 431
PkrI GCNGC 1 cut(s) 545
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
Ppu21I YACGTR 1 cut(s) 581
PshBI ATTAAT 1 cut(s) 461
Psp6I CCWGG 1 cut(s) 526
PspGI CCWGG 1 cut(s) 526
PspPI GGNCC 1 cut(s) 472
PsuI RGATCY 1 cut(s) 497
PvuII CAGCTG 1 cut(s) 543
RsaI GTAC 6 cut(s) 239, 263, 309, 341, 350, 576
RsaNI GTAC 6 cut(s) 238, 262, 308, 340, 349, 575
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 3 cut(s) 93, 461, 552
SatI GCNGC 1 cut(s) 544
Sau3AI GATC 3 cut(s) 358, 497, 523
Sau96I GGNCC 1 cut(s) 472
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 528
SduI GDGCHC 1 cut(s) 212
SetI ASST 4 cut(s) 283, 545, 583, 600
SfaNI GCATC 3 cut(s) 76, 103, 404
Sse9I AATT 3 cut(s) 173, 344, 375
SsiI CCGC 1 cut(s) 393
SspI AATATT 1 cut(s) 304
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 1 cut(s) 526
TaaI ACNGT 2 cut(s) 521, 574
TaiI ACGT 1 cut(s) 583
TaqI TCGA 2 cut(s) 171, 357
TaqII GACCGA 1 cut(s) 248
TasI AATT 3 cut(s) 173, 344, 375
TatI WGTACW 3 cut(s) 237, 339, 348
Tru1I TTAA 3 cut(s) 93, 461, 552
Tru9I TTAA 3 cut(s) 93, 461, 552
TscAI CASTG 1 cut(s) 123
TseI GCWGC 1 cut(s) 543
TspDTI ATGAA 2 cut(s) 20, 54
TspGWI ACGGA 2 cut(s) 216, 391
TspRI CASTG 1 cut(s) 123
VspI ATTAAT 1 cut(s) 461
XapI RAATTY 1 cut(s) 173
XspI CTAG 1 cut(s) 254
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.