Rmu_sc0008973.1_g000018

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008973.1
OG0000345
Physical Location & Seq
Reverse (-)
86733 .. 87032
300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008973.1_g000018.1.cds

Sequence Viewer

Length: 300 bp
atgtctgctaccgaaaaccatggtttgaggtactattttctgccggtagcatggccccaattgatatctcattattccgatatggacttctgggaaacagagtacaatagccatggtacttgctcgaagaacaatctgagcgagacggaatattttaagaaagcttactggatgtggtatcaatatcatgcatatcagctgtctgcaatcgcaccgtcaccaatctaccctggaaattactattatcgcattgaccttgaaaatgccattcagcgagtgactgtaccagcctctgcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

99

Amino Acids

11.77

Weight (kDa)

5.69

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 4 cut(s) 32, 104, 118, 285
AgsI TTSAA 1 cut(s) 260
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 2 cut(s) 164, 199
AluI AGCT 2 cut(s) 164, 199
Alw26I GTCTC 1 cut(s) 137
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 210
BciT130I CCWGG 1 cut(s) 231
BcoDI GTCTC 1 cut(s) 137
Bme1390I CCNGG 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 56
BmrFI CCNGG 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 19, 112, 229
Bse118I RCCGGY 1 cut(s) 43
Bse1I ACTGG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 3 cut(s) 19, 112, 229
BseGI GGATG 1 cut(s) 177
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 173
BshFI GGCC 1 cut(s) 55
BsiSI CCGG 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 137
BsmBI CGTCTC 1 cut(s) 137
BsnI GGCC 1 cut(s) 55
Bsp19I CCATGG 2 cut(s) 19, 112
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 1 cut(s) 129
BspLI GGNNCC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 43
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 43
BssECI CCNNGG 3 cut(s) 19, 112, 229
BssT1I CCWWGG 2 cut(s) 19, 112
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 2 cut(s) 216, 283
BstDEI CTNAG 2 cut(s) 137, 297
BstDSI CCRYGG 2 cut(s) 19, 112
BstF5I GGATG 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 137
BstNI CCWGG 1 cut(s) 231
BstSCI CCNGG 1 cut(s) 229
BsuRI GGCC 1 cut(s) 55
BtgI CCRYGG 2 cut(s) 19, 112
BtsCI GGATG 1 cut(s) 177
CaiI CAGNNNCTG 1 cut(s) 293
Cfr10I RCCGGY 1 cut(s) 43
Cfr13I GGNCC 1 cut(s) 54
Csp6I GTAC 4 cut(s) 31, 103, 117, 284
CviAII CATG 4 cut(s) 20, 51, 113, 188
CviJI RGCY 5 cut(s) 55, 111, 164, 199, 290
CviKI_1 RGCY 5 cut(s) 55, 111, 164, 199, 290
CviQI GTAC 4 cut(s) 31, 103, 117, 284
DdeI CTNAG 2 cut(s) 137, 297
Eco130I CCWWGG 2 cut(s) 19, 112
Eco32I GATATC 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 229
EcoRV GATATC 1 cut(s) 66
EcoT14I CCWWGG 2 cut(s) 19, 112
EcoT22I ATGCAT 1 cut(s) 193
ErhI CCWWGG 2 cut(s) 19, 112
Esp3I CGTCTC 1 cut(s) 137
FaeI CATG 4 cut(s) 23, 54, 116, 191
FaiI YATR 6 cut(s) 21, 52, 83, 114, 189, 193
FatI CATG 4 cut(s) 19, 50, 112, 187
FokI GGATG 1 cut(s) 184
HaeIII GGCC 1 cut(s) 55
HapII CCGG 1 cut(s) 44
Hin1II CATG 4 cut(s) 23, 54, 116, 191
HindIII AAGCTT 1 cut(s) 162
HpaII CCGG 1 cut(s) 44
HphI GGTGA 1 cut(s) 210
Hpy188I TCNGA 2 cut(s) 79, 138
HpyCH4III ACNGT 2 cut(s) 216, 283
HpyCH4V TGCA 2 cut(s) 191, 206
HpyF3I CTNAG 2 cut(s) 137, 297
Hsp92II CATG 4 cut(s) 23, 54, 116, 191
LpnPI CCDG 5 cut(s) 57, 76, 154, 216, 243
MaeIII GTNAC 2 cut(s) 216, 277
MboII GAAGA 1 cut(s) 139
MfeI CAATTG 1 cut(s) 59
MluCI AATT 2 cut(s) 59, 235
MnlI CCTC 1 cut(s) 21
Mph1103I ATGCAT 1 cut(s) 193
MseI TTAA 1 cut(s) 156
MspA1I CMGCKG 1 cut(s) 199
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 231
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 1 cut(s) 231
NcoI CCATGG 2 cut(s) 19, 112
NlaIII CATG 4 cut(s) 23, 54, 116, 191
NlaIV GGNNCC 1 cut(s) 56
NmuCI GTSAC 2 cut(s) 216, 277
NsiI ATGCAT 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 1 cut(s) 56
PspPI GGNCC 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 293
PvuII CAGCTG 1 cut(s) 199
RsaI GTAC 4 cut(s) 32, 104, 118, 285
RsaNI GTAC 4 cut(s) 31, 103, 117, 284
SaqAI TTAA 1 cut(s) 156
Sau96I GGNCC 1 cut(s) 54
ScrFI CCNGG 1 cut(s) 231
SetI ASST 4 cut(s) 32, 166, 201, 258
Sse9I AATT 2 cut(s) 59, 235
SspI AATATT 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 2 cut(s) 19, 112
TaaI ACNGT 2 cut(s) 216, 283
TaqI TCGA 1 cut(s) 125
TasI AATT 2 cut(s) 59, 235
TatI WGTACW 1 cut(s) 102
Tru1I TTAA 1 cut(s) 156
Tru9I TTAA 1 cut(s) 156
TseFI GTSAC 2 cut(s) 216, 277
Tsp45I GTSAC 2 cut(s) 216, 277
TspGWI ACGGA 1 cut(s) 161
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.