Rw6G030670

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000345
Physical Location & Seq
Forward (+)
54787014 .. 54787756
743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G030670.1

Sequence Viewer

Length: 624 bp
ATGTCTCTAGGTTCATATGATCATTTTAAATTTGTACAACAATGGCCACCGGCCGTCTGTGATACGTCTGGCTGCAAGAGATCGGGGTTATCACACTTCACGATCCATGGCCTTTGGCCAAATAATAAAACGTATGTGAAAAATCAACCGACTTGTCTTACCAGTCATGCCAACTCATTTAAAGCTACGATACTCACTAGTTCGAATTTGGTAAGCAAGCTGAGCACCTCTTGGCCCGATGTGAAAAATGCAAATGATAATGATTTTTGGAACAAACAATGGGACAAGCATGGCACATGTTCCTTGCAGACATACGACCAAGCACAATACTTCGAGCGGTCATACAACATGTGGAAAGAAACCAACCTGACAAACACCTTGAATAGCCTCATCAAACAGACACCGAGACAACAGAACGTCACAGATATAGAACAACTCATTCAAGGAGTAACTGCAACTGAAAAGAAACCCCTCCTCCGTTGTGAACAATCTCATAGTAATGCCAGTAAGTCTCTCTTGAAAGAGATTGTCATTTGCTATGCACACAACGGGATTACTGTGATCGATTGCGTCAGTCGACCAAAATCCACCTTCGAATGCTATGACGGATTTTGGTTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

207

Amino Acids

23.68

Weight (kDa)

8.58

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 6 - 190 2e-36 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 337
AccI GTMKAC 1 cut(s) 577
AciI CCGC 1 cut(s) 337
AclWI GGATC 1 cut(s) 97
AcoI YGGCCR 3 cut(s) 44, 51, 116
AcsI RAATTY 2 cut(s) 29, 205
AfaI GTAC 1 cut(s) 36
AflIII ACRYGT 2 cut(s) 296, 348
AgsI TTSAA 3 cut(s) 382, 443, 520
AhlI ACTAGT 1 cut(s) 197
AluBI AGCT 2 cut(s) 185, 220
AluI AGCT 2 cut(s) 185, 220
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 3 cut(s) 9, 400, 516
AlwI GGATC 1 cut(s) 97
AoxI GGCC 5 cut(s) 44, 51, 109, 116, 233
ApeKI GCWGC 1 cut(s) 72
ApoI RAATTY 2 cut(s) 29, 205
AspS9I GGNCC 1 cut(s) 234
AsuII TTCGAA 2 cut(s) 203, 594
BalI TGGCCA 2 cut(s) 46, 118
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 1 cut(s) 59
BceAI ACGGC 1 cut(s) 38
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 3 cut(s) 9, 400, 516
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 2 cut(s) 8, 198
BisI GCNGC 1 cut(s) 73
BlpI GCTNAGC 1 cut(s) 221
BlsI GCNGC 1 cut(s) 74
BmgT120I GGNCC 1 cut(s) 234
Bpu1102I GCTNAGC 1 cut(s) 221
Bpu14I TTCGAA 2 cut(s) 203, 594
Bsa29I ATCGAT 1 cut(s) 564
BsaJI CCNNGG 1 cut(s) 106
BsaXI ACNNNNNCTCC 2 cut(s) 459, 489
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 2 cut(s) 162, 504
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 1 cut(s) 106
BseMII CTCAG 1 cut(s) 212
BseNI ACTGG 2 cut(s) 162, 504
BseRI GAGGAG 1 cut(s) 464
BseX3I CGGCCG 1 cut(s) 51
BseXI GCAGC 1 cut(s) 59
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 5 cut(s) 46, 53, 111, 118, 235
BshVI ATCGAT 1 cut(s) 564
BsiEI CGRYCG 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 296
BsmAI GTCTC 3 cut(s) 9, 400, 516
BsmFI GGGAC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 602
BsnI GGCC 5 cut(s) 46, 53, 111, 118, 235
Bsp119I TTCGAA 2 cut(s) 203, 594
Bsp1286I GDGCHC 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 34
Bsp143I GATC 4 cut(s) 19, 80, 102, 561
Bsp1720I GCTNAGC 1 cut(s) 221
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 1 cut(s) 337
BspANI GGCC 5 cut(s) 46, 53, 111, 118, 235
BspCNI CTCAG 1 cut(s) 213
BspDI ATCGAT 1 cut(s) 564
BspPI GGATC 1 cut(s) 97
BspT104I TTCGAA 2 cut(s) 203, 594
BsrBI CCGCTC 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 49
BsrGI TGTACA 1 cut(s) 34
BsrI ACTGG 2 cut(s) 162, 504
BssAI RCCGGY 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 4 cut(s) 19, 80, 102, 561
BssT1I CCWWGG 1 cut(s) 106
Bst4CI ACNGT 1 cut(s) 559
BstAUI TGTACA 1 cut(s) 34
BstBI TTCGAA 2 cut(s) 203, 594
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 1 cut(s) 221
BstDSI CCRYGG 1 cut(s) 106
BstKTI GATC 4 cut(s) 22, 83, 105, 564
BstMAI GTCTC 3 cut(s) 9, 400, 516
BstMBI GATC 4 cut(s) 19, 80, 102, 561
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstNSI RCATGY 2 cut(s) 300, 352
BstV1I GCAGC 1 cut(s) 59
BstZI CGGCCG 1 cut(s) 51
Bsu15I ATCGAT 1 cut(s) 564
BsuRI GGCC 5 cut(s) 46, 53, 111, 118, 235
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 218
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 234
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 1 cut(s) 559
Csp6I GTAC 1 cut(s) 35
CviAII CATG 5 cut(s) 107, 167, 290, 297, 349
CviJI RGCY 9 cut(s) 46, 53, 72, 111, 118, 185, 220, 235, 387
CviKI_1 RGCY 9 cut(s) 46, 53, 72, 111, 118, 185, 220, 235, 387
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 1 cut(s) 221
DpnI GATC 4 cut(s) 21, 82, 104, 563
DpnII GATC 4 cut(s) 19, 80, 102, 561
DraI TTTAAA 2 cut(s) 28, 181
EaeI YGGCCR 3 cut(s) 44, 51, 116
EagI CGGCCG 1 cut(s) 51
EclXI CGGCCG 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 51
EcoT14I CCWWGG 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 106
FaeI CATG 5 cut(s) 110, 170, 293, 300, 352
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 1 cut(s) 296
FatI CATG 5 cut(s) 106, 166, 289, 296, 348
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 19
FblI GTMKAC 1 cut(s) 577
Fnu4HI GCNGC 1 cut(s) 73
Fsp4HI GCNGC 1 cut(s) 73
FspBI CTAG 2 cut(s) 8, 198
GluI GCNGC 1 cut(s) 73
HaeIII GGCC 5 cut(s) 46, 53, 111, 118, 235
HapII CCGG 1 cut(s) 50
HgaI GACGC 1 cut(s) 559
Hin1II CATG 5 cut(s) 110, 170, 293, 300, 352
HincII GTYRAC 1 cut(s) 578
HindII GTYRAC 1 cut(s) 578
HpaII CCGG 1 cut(s) 50
Hpy166II GTNNAC 2 cut(s) 485, 578
Hpy188III TCNNGA 2 cut(s) 100, 517
Hpy8I GTNNAC 2 cut(s) 485, 578
HpyAV CCTTC 1 cut(s) 601
HpyCH4III ACNGT 1 cut(s) 559
HpyCH4IV ACGT 3 cut(s) 65, 131, 417
HpyCH4V TGCA 5 cut(s) 75, 251, 307, 455, 542
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 1 cut(s) 221
HpySE526I ACGT 3 cut(s) 65, 131, 417
Hsp92II CATG 5 cut(s) 110, 170, 293, 300, 352
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 4 cut(s) 19, 80, 102, 561
LpnPI CCDG 5 cut(s) 54, 63, 175, 380, 517
Lsp1109I GCAGC 1 cut(s) 59
MaeI CTAG 2 cut(s) 8, 198
MaeII ACGT 3 cut(s) 65, 131, 417
MaeIII GTNAC 2 cut(s) 418, 448
MalI GATC 4 cut(s) 21, 82, 104, 563
MbiI CCGCTC 1 cut(s) 337
MboI GATC 4 cut(s) 19, 80, 102, 561
MhlI GDGCHC 1 cut(s) 227
MlsI TGGCCA 2 cut(s) 46, 118
MluCI AATT 2 cut(s) 29, 205
MluNI TGGCCA 2 cut(s) 46, 118
MnlI CCTC 4 cut(s) 238, 398, 482, 485
Mox20I TGGCCA 2 cut(s) 46, 118
MscI TGGCCA 2 cut(s) 46, 118
MseI TTAA 2 cut(s) 27, 180
MslI CAYNNNNRTG 1 cut(s) 498
Msp20I TGGCCA 2 cut(s) 46, 118
MspI CCGG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 602
MwoI GCNNNNNNNGC 1 cut(s) 222
NcoI CCATGG 1 cut(s) 106
NdeI CATATG 1 cut(s) 16
NdeII GATC 4 cut(s) 19, 80, 102, 561
NlaIII CATG 5 cut(s) 110, 170, 293, 300, 352
NmuCI GTSAC 1 cut(s) 418
NspI RCATGY 2 cut(s) 300, 352
NspV TTCGAA 2 cut(s) 203, 594
PciI ACATGT 2 cut(s) 296, 348
PctI GAATGC 1 cut(s) 602
PkrI GCNGC 1 cut(s) 74
PscI ACATGT 2 cut(s) 296, 348
PspPI GGNCC 1 cut(s) 234
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
RseI CAYNNNNRTG 1 cut(s) 498
SalI GTCGAC 1 cut(s) 576
SaqAI TTAA 2 cut(s) 27, 180
SatI GCNGC 1 cut(s) 73
Sau3AI GATC 4 cut(s) 19, 80, 102, 561
Sau96I GGNCC 1 cut(s) 234
SduI GDGCHC 1 cut(s) 227
SfuI TTCGAA 2 cut(s) 203, 594
SmiMI CAYNNNNRTG 1 cut(s) 498
SpeI ACTAGT 1 cut(s) 197
Sse9I AATT 2 cut(s) 29, 205
SsiI CCGC 1 cut(s) 337
SspMI CTAG 2 cut(s) 8, 198
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 1 cut(s) 559
TaiI ACGT 3 cut(s) 68, 134, 420
TaqI TCGA 5 cut(s) 203, 333, 564, 577, 594
TasI AATT 2 cut(s) 29, 205
TatI WGTACW 1 cut(s) 34
Tru1I TTAA 2 cut(s) 27, 180
Tru9I TTAA 2 cut(s) 27, 180
TseFI GTSAC 1 cut(s) 418
TseI GCWGC 1 cut(s) 72
Tsp45I GTSAC 1 cut(s) 418
TspGWI ACGGA 2 cut(s) 467, 621
XapI RAATTY 2 cut(s) 29, 205
XceI RCATGY 2 cut(s) 300, 352
XmiI GTMKAC 1 cut(s) 577
XspI CTAG 2 cut(s) 8, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.