Rh2AG218400

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000345
Physical Location & Seq
Forward (+)
22445468 .. 22453978
8511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG218400.1

Sequence Viewer

Length: 669 bp
ATGACACCTCTGCAGACGGATTTAGTAAACTCATGGCCGTCCGTGTTAACAACAAAGAGCAATATGTGGTTCTGGGAGCATGAGTACGAGAACCATGGCGCATGTACAGTAGAGTCAGGTGTACCACCTTTCACTCAAAAGTCCTACTTTAAGAAAGGACACCAGCTATGGAACCAATATGACATCCATTCAGTGCTTGATCAAAGTGGTATTAAACCGAGTACTGCAAAATCATACACTATGACTCAGCTTGTAACCGCAATCAAAAAGAAAATCGGGAGTAACAATACCCCTTTAATCATGTGCAGGAAGAAAAAACTTGGTTACATACTAAGGGAAGTGATAATCTGTCTGGATCACCAGGCAACAAATGTGATAAGTTGCGCGCTCAGTAAAAGGAAAACAGACTACCTGGATCCTTCGGGCAAGCTAAGGCATCAAGCACATCTTTCTGTTTTGGACAACACAGCTGAAGTTCAGCCATATATAGATGAGCATTTGGAGAGATTGAAACTGGAGCACCCTCAAAAGTCTAAGGCAGAAAATTGGCTTAAAGATGAACATAATCGAAGATTTAGCAATTGGCTGCAACAAAGAGTATGTAGAATAGGGCAGATTCTTCTTCATTCTAGCATTATTGTTTATCTTGTAATAATGTTCAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

222

Amino Acids

25.77

Weight (kDa)

9.3

Isoelectric Point (pI)

28.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 3 - 128 1.1e-27 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 386
AciI CCGC 1 cut(s) 258
AclWI GGATC 3 cut(s) 363, 410, 423
AcoI YGGCCR 1 cut(s) 35
AcuI CTGAAG 1 cut(s) 492
AfaI GTAC 4 cut(s) 86, 106, 123, 223
AgsI TTSAA 2 cut(s) 511, 661
AjnI CCWGG 2 cut(s) 360, 411
AluBI AGCT 4 cut(s) 166, 250, 430, 470
AluI AGCT 4 cut(s) 166, 250, 430, 470
Alw21I GWGCWC 1 cut(s) 522
AlwI GGATC 3 cut(s) 363, 410, 423
AoxI GGCC 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 586
AspLEI GCGC 3 cut(s) 101, 386, 388
AsuHPI GGTGA 1 cut(s) 350
BamHI GGATCC 1 cut(s) 415
Bbv12I GWGCWC 1 cut(s) 522
BbvI GCAGC 1 cut(s) 573
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 2 cut(s) 362, 413
BclI TGATCA 1 cut(s) 199
BfaI CTAG 1 cut(s) 630
BfmI CTRYAG 1 cut(s) 11
BisI GCNGC 1 cut(s) 587
BlsI GCNGC 1 cut(s) 588
BmcAI AGTACT 1 cut(s) 223
Bme1390I CCNGG 2 cut(s) 362, 413
BmiI GGNNCC 2 cut(s) 173, 417
BmrFI CCNGG 2 cut(s) 362, 413
BmsI GCATC 1 cut(s) 445
BpmI CTGGAG 1 cut(s) 536
Bpu10I CCTNAGC 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 68, 98
Bse1I ACTGG 1 cut(s) 519
BseBI CCWGG 2 cut(s) 362, 413
BseDI CCNNGG 1 cut(s) 94
BseGI GGATG 1 cut(s) 183
BseMII CTCAG 2 cut(s) 260, 403
BseNI ACTGG 1 cut(s) 519
BsePI GCGCGC 1 cut(s) 384
BseXI GCAGC 1 cut(s) 573
BsgI GTGCAG 1 cut(s) 325
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 522
BsnI GGCC 1 cut(s) 37
Bsp1286I GDGCHC 1 cut(s) 522
Bsp1407I TGTACA 1 cut(s) 104
Bsp143I GATC 3 cut(s) 199, 355, 415
Bsp19I CCATGG 1 cut(s) 94
BspACI CCGC 1 cut(s) 258
BspANI GGCC 1 cut(s) 37
BspCNI CTCAG 2 cut(s) 259, 402
BspFNI CGCG 1 cut(s) 386
BspLI GGNNCC 2 cut(s) 173, 417
BspMAI CTGCAG 1 cut(s) 15
BspPI GGATC 3 cut(s) 363, 410, 423
BsrGI TGTACA 1 cut(s) 104
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 1 cut(s) 94
BssHII GCGCGC 1 cut(s) 384
BssMI GATC 3 cut(s) 199, 355, 415
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 2 cut(s) 362, 413
Bst4CI ACNGT 1 cut(s) 109
BstAUI TGTACA 1 cut(s) 104
BstC8I GCNNGC 2 cut(s) 386, 428
BstDEI CTNAG 5 cut(s) 246, 332, 389, 431, 534
BstDSI CCRYGG 1 cut(s) 94
BstF5I GGATG 1 cut(s) 183
BstFNI CGCG 1 cut(s) 386
BstHHI GCGC 3 cut(s) 101, 386, 388
BstKTI GATC 3 cut(s) 202, 358, 418
BstMBI GATC 3 cut(s) 199, 355, 415
BstNI CCWGG 2 cut(s) 362, 413
BstNSI RCATGY 1 cut(s) 105
BstSCI CCNGG 2 cut(s) 360, 411
BstSFI CTRYAG 1 cut(s) 11
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 1 cut(s) 573
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 1 cut(s) 37
BtgI CCRYGG 1 cut(s) 94
BtsCI GGATG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 386, 428
CfoI GCGC 3 cut(s) 101, 386, 388
Csp6I GTAC 4 cut(s) 85, 105, 122, 222
CviAII CATG 5 cut(s) 33, 80, 95, 102, 301
CviJI RGCY 8 cut(s) 37, 166, 250, 430, 470, 481, 550, 586
CviKI_1 RGCY 8 cut(s) 37, 166, 250, 430, 470, 481, 550, 586
CviQI GTAC 4 cut(s) 85, 105, 122, 222
DdeI CTNAG 5 cut(s) 246, 332, 389, 431, 534
DpnI GATC 3 cut(s) 201, 357, 417
DpnII GATC 3 cut(s) 199, 355, 415
EaeI YGGCCR 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 94
Eco57I CTGAAG 1 cut(s) 492
EcoRII CCWGG 2 cut(s) 360, 411
EcoT14I CCWWGG 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 94
FaeI CATG 5 cut(s) 36, 83, 98, 105, 304
FalI AAGNNNNNCTT 4 cut(s) 131, 163, 432, 464
FatI CATG 5 cut(s) 32, 79, 94, 101, 300
FbaI TGATCA 1 cut(s) 199
Fnu4HI GCNGC 1 cut(s) 587
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 1 cut(s) 587
FspBI CTAG 1 cut(s) 630
GlaI GCGC 3 cut(s) 100, 385, 387
GluI GCNGC 1 cut(s) 587
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 1 cut(s) 37
HhaI GCGC 3 cut(s) 101, 386, 388
Hin1II CATG 5 cut(s) 36, 83, 98, 105, 304
Hin6I GCGC 3 cut(s) 99, 384, 386
HinP1I GCGC 3 cut(s) 99, 384, 386
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 3 cut(s) 113, 244, 616
HpaI GTTAAC 1 cut(s) 48
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 3 cut(s) 28, 48, 122
Hpy188III TCNNGA 2 cut(s) 277, 353
Hpy8I GTNNAC 3 cut(s) 28, 48, 122
HpyAV CCTTC 1 cut(s) 429
HpyCH4III ACNGT 1 cut(s) 109
HpyCH4V TGCA 4 cut(s) 13, 227, 306, 589
HpyF3I CTNAG 5 cut(s) 246, 332, 389, 431, 534
Hsp92II CATG 5 cut(s) 36, 83, 98, 105, 304
HspAI GCGC 3 cut(s) 99, 384, 386
Ksp22I TGATCA 1 cut(s) 199
KspAI GTTAAC 1 cut(s) 48
Kzo9I GATC 3 cut(s) 199, 355, 415
LmnI GCTCC 2 cut(s) 76, 517
Lsp1109I GCAGC 1 cut(s) 573
LweI GCATC 1 cut(s) 445
MaeI CTAG 1 cut(s) 630
MaeIII GTNAC 3 cut(s) 253, 281, 323
MalI GATC 3 cut(s) 201, 357, 417
MboI GATC 3 cut(s) 199, 355, 415
MboII GAAGA 4 cut(s) 322, 582, 611, 614
MfeI CAATTG 1 cut(s) 580
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 522
MluCI AATT 2 cut(s) 544, 580
MlyI GAGTC 2 cut(s) 122, 238
MnlI CCTC 2 cut(s) 18, 534
MseI TTAA 6 cut(s) 47, 150, 213, 296, 552, 667
MspA1I CMGCKG 1 cut(s) 470
MspR9I CCNGG 2 cut(s) 362, 413
MunI CAATTG 1 cut(s) 580
MvaI CCWGG 2 cut(s) 362, 413
MvnI CGCG 1 cut(s) 386
NcoI CCATGG 1 cut(s) 94
NdeII GATC 3 cut(s) 199, 355, 415
NlaIII CATG 5 cut(s) 36, 83, 98, 105, 304
NlaIV GGNNCC 2 cut(s) 173, 417
NspI RCATGY 1 cut(s) 105
PauI GCGCGC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 616
PkrI GCNGC 1 cut(s) 588
PleI GAGTC 2 cut(s) 121, 238
PpsI GAGTC 2 cut(s) 121, 238
Psp6I CCWGG 2 cut(s) 360, 411
PspGI CCWGG 2 cut(s) 360, 411
PspN4I GGNNCC 2 cut(s) 173, 417
PstI CTGCAG 1 cut(s) 15
PsuI RGATCY 1 cut(s) 415
PteI GCGCGC 1 cut(s) 384
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 4 cut(s) 86, 106, 123, 223
RsaNI GTAC 4 cut(s) 85, 105, 122, 222
SaqAI TTAA 6 cut(s) 47, 150, 213, 296, 552, 667
SatI GCNGC 1 cut(s) 587
Sau3AI GATC 3 cut(s) 199, 355, 415
ScaI AGTACT 1 cut(s) 223
SchI GAGTC 2 cut(s) 122, 238
ScrFI CCNGG 2 cut(s) 362, 413
SduI GDGCHC 1 cut(s) 522
SetI ASST 9 cut(s) 10, 121, 130, 168, 252, 414, 432, 472, 666
SfaNI GCATC 1 cut(s) 445
SfcI CTRYAG 1 cut(s) 11
Sse9I AATT 2 cut(s) 544, 580
SsiI CCGC 1 cut(s) 258
SspMI CTAG 1 cut(s) 630
StyD4I CCNGG 2 cut(s) 360, 411
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 109
TaqI TCGA 1 cut(s) 568
TasI AATT 2 cut(s) 544, 580
TatI WGTACW 2 cut(s) 104, 221
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 6 cut(s) 47, 150, 213, 296, 552, 667
Tru9I TTAA 6 cut(s) 47, 150, 213, 296, 552, 667
TscAI CASTG 1 cut(s) 198
TseI GCWGC 1 cut(s) 586
TspDTI ATGAA 2 cut(s) 573, 614
TspGWI ACGGA 2 cut(s) 31, 32
TspRI CASTG 1 cut(s) 198
XceI RCATGY 1 cut(s) 105
XspI CTAG 1 cut(s) 630
ZrmI AGTACT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.