RLG00000024924

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000345
Physical Location & Seq
Reverse (-)
40375447 .. 40376240
794 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024924

Sequence Viewer

Length: 693 bp
ATGGATATGGCATTGCTAAGTACAATAGCCGTGCTGCTTGTTTCTGCTTTTTCATCAGGTGCTAAAGCCGCCAATAAGTACGACTACTTCCAGTTTGTCCAGCAATGGCCGGGTACATTCTGCTATCAAAATTCGGATTGTGTCCGGAATCCCCCGCGGTATACTTTTACCATCCACGGCCTCTGGCCAAGTAATTACACGGCACCTGAAAAGCCATGCGTTGGCACACCCTTTAGTCCAGCTGAGATGTTTGCTGCCCCAAACCATGGTTTGAAAGACTATTTTCTGCCTCAAGCATGGCCCCAGTTAATTGCTCGTTTCACCGACATGCAATTCTGGCAGACCGAATATAACAAGCACGGTACCTGCTCGGAGAACAAACTCAAGCAGACGGAGTATTTTAGGACAGCTCACTGGGCGTGGGTTCGATTCAATGCATATTTTCTGTTTGCACACTCGCCTCTCCAAATCCACCCTGGTAATTACTATCTTAAAGCTGACCTTGAACAAGCCATTCGGGCAGTCACTACTAAGGACCCTTTGCTTGTGTGCAAGAAGATCAAACCCATGGGCATTGATACGTGGCTATTGCAGGAAGTCATAATCTGTCTTGATGTTTTGGGAGTCCAACCGGTCCCTTGTCGCCCTCGATTACCAACTTGCCGTGGTCCAATAATATATTATGGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

231

Amino Acids

26.46

Weight (kDa)

8.72

Isoelectric Point (pI)

30.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 214 9.6e-33 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 374
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 2 cut(s) 202, 362
AccB7I CCANNNNNTGG 2 cut(s) 221, 266
AccI GTMKAC 1 cut(s) 161
AccII CGCG 1 cut(s) 157
AccIII TCCGGA 1 cut(s) 144
AciI CCGC 3 cut(s) 69, 155, 157
AcoI YGGCCR 2 cut(s) 107, 185
AcsI RAATTY 1 cut(s) 130
AfaI GTAC 4 cut(s) 22, 80, 115, 364
AfiI CCNNNNNNNGG 2 cut(s) 221, 266
AgeI ACCGGT 1 cut(s) 631
AgsI TTSAA 3 cut(s) 274, 433, 506
AjnI CCWGG 1 cut(s) 475
AluBI AGCT 3 cut(s) 242, 410, 497
AluI AGCT 3 cut(s) 242, 410, 497
Aor13HI TCCGGA 1 cut(s) 144
AoxI GGCC 4 cut(s) 107, 178, 185, 299
ApeKI GCWGC 2 cut(s) 34, 254
ApoI RAATTY 1 cut(s) 130
AsiGI ACCGGT 1 cut(s) 631
Asp718I GGTACC 1 cut(s) 362
AspS9I GGNCC 4 cut(s) 300, 535, 634, 668
AsuC2I CCSGG 1 cut(s) 111
AsuHPI GGTGA 1 cut(s) 313
AvaII GGWCC 3 cut(s) 535, 634, 668
BalI TGGCCA 1 cut(s) 187
BanI GGYRCC 2 cut(s) 202, 362
BarI GAAGNNNNNNTAC 2 cut(s) 71, 103
BbvI GCAGC 2 cut(s) 21, 241
BccI CCATC 1 cut(s) 179
BceAI ACGGC 4 cut(s) 14, 193, 216, 648
BciT130I CCWGG 1 cut(s) 477
BcnI CCSGG 1 cut(s) 111
BfuAI ACCTGC 1 cut(s) 374
BglI GCCNNNNNGGC 1 cut(s) 518
BisI GCNGC 3 cut(s) 35, 69, 255
BlsI GCNGC 3 cut(s) 36, 70, 256
Bme1390I CCNGG 2 cut(s) 111, 477
Bme18I GGWCC 3 cut(s) 535, 634, 668
BmgT120I GGNCC 4 cut(s) 300, 535, 634, 668
BmiI GGNNCC 5 cut(s) 204, 302, 364, 537, 636
BmrFI CCNGG 2 cut(s) 111, 477
BmrI ACTGGG 2 cut(s) 298, 424
BmuI ACTGGG 2 cut(s) 298, 424
BpuEI CTTGAG 2 cut(s) 276, 368
BpuMI CCSGG 1 cut(s) 111
BsaAI YACGTR 1 cut(s) 582
BsaJI CCNNGG 6 cut(s) 155, 175, 265, 475, 567, 664
BsaWI WCCGGW 2 cut(s) 144, 631
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 266
Bse118I RCCGGY 1 cut(s) 631
Bse1I ACTGG 3 cut(s) 91, 304, 419
Bse3DI GCAATG 2 cut(s) 11, 110
BseAI TCCGGA 1 cut(s) 144
BseBI CCWGG 1 cut(s) 477
BseDI CCNNGG 6 cut(s) 155, 175, 265, 475, 567, 664
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 221, 266
BseMI GCAATG 2 cut(s) 11, 110
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 3 cut(s) 91, 304, 419
BseXI GCAGC 2 cut(s) 21, 241
Bsh1236I CGCG 1 cut(s) 157
BshFI GGCC 4 cut(s) 109, 180, 187, 301
BshNI GGYRCC 2 cut(s) 202, 362
BshTI ACCGGT 1 cut(s) 631
BsiSI CCGG 3 cut(s) 110, 145, 632
BslFI GGGAC 1 cut(s) 620
BslI CCNNNNNNNGG 2 cut(s) 221, 266
BsmFI GGGAC 1 cut(s) 620
BsnI GGCC 4 cut(s) 109, 180, 187, 301
Bsp13I TCCGGA 1 cut(s) 144
Bsp143I GATC 1 cut(s) 558
Bsp19I CCATGG 2 cut(s) 265, 567
BspACI CCGC 3 cut(s) 69, 155, 157
BspANI GGCC 4 cut(s) 109, 180, 187, 301
BspCNI CTCAG 1 cut(s) 235
BspEI TCCGGA 1 cut(s) 144
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 5 cut(s) 204, 302, 364, 537, 636
BspMI ACCTGC 1 cut(s) 374
BspT107I GGYRCC 2 cut(s) 202, 362
BsrDI GCAATG 2 cut(s) 11, 110
BsrFI RCCGGY 1 cut(s) 631
BsrI ACTGG 3 cut(s) 91, 304, 419
BssAI RCCGGY 1 cut(s) 631
BssECI CCNNGG 6 cut(s) 155, 175, 265, 475, 567, 664
BssMI GATC 1 cut(s) 558
BssNAI GTATAC 1 cut(s) 162
BssT1I CCWWGG 2 cut(s) 265, 567
Bst1107I GTATAC 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 477
Bst4CI ACNGT 1 cut(s) 362
BstBAI YACGTR 1 cut(s) 582
BstDEI CTNAG 3 cut(s) 17, 243, 531
BstDSI CCRYGG 5 cut(s) 155, 175, 265, 567, 664
BstF5I GGATG 1 cut(s) 171
BstFNI CGCG 1 cut(s) 157
BstKTI GATC 1 cut(s) 561
BstMBI GATC 1 cut(s) 558
BstMWI GCNNNNNNNGC 4 cut(s) 68, 337, 416, 518
BstNI CCWGG 1 cut(s) 477
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 2 cut(s) 109, 475
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 21, 241
BstZ17I GTATAC 1 cut(s) 162
BsuRI GGCC 4 cut(s) 109, 180, 187, 301
BtgI CCRYGG 5 cut(s) 155, 175, 265, 567, 664
BtsCI GGATG 1 cut(s) 171
BtsIMutI CAGTG 1 cut(s) 412
BveI ACCTGC 1 cut(s) 374
Cfr10I RCCGGY 1 cut(s) 631
Cfr13I GGNCC 4 cut(s) 300, 535, 634, 668
Cfr42I CCGCGG 1 cut(s) 158
Csp6I GTAC 4 cut(s) 21, 79, 114, 363
CspAI ACCGGT 1 cut(s) 631
CviAII CATG 5 cut(s) 216, 266, 297, 328, 568
CviQI GTAC 4 cut(s) 21, 79, 114, 363
DdeI CTNAG 3 cut(s) 17, 243, 531
DpnI GATC 1 cut(s) 560
DpnII GATC 1 cut(s) 558
EaeI YGGCCR 2 cut(s) 107, 185
Eco130I CCWWGG 2 cut(s) 265, 567
Eco47I GGWCC 3 cut(s) 535, 634, 668
EcoO109I RGGNCCY 1 cut(s) 535
EcoRII CCWGG 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 265, 567
EcoT22I ATGCAT 1 cut(s) 439
ErhI CCWWGG 2 cut(s) 265, 567
FaeI CATG 5 cut(s) 219, 269, 300, 331, 571
FalI AAGNNNNNCTT 2 cut(s) 486, 518
FaqI GGGAC 1 cut(s) 620
FatI CATG 5 cut(s) 215, 265, 296, 327, 567
FauI CCCGC 1 cut(s) 162
FblI GTMKAC 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 35, 69, 255
FokI GGATG 1 cut(s) 158
Fsp4HI GCNGC 3 cut(s) 35, 69, 255
GluI GCNGC 3 cut(s) 35, 69, 255
HaeIII GGCC 4 cut(s) 109, 180, 187, 301
HapII CCGG 3 cut(s) 110, 145, 632
Hin1II CATG 5 cut(s) 219, 269, 300, 331, 571
HinfI GANTC 3 cut(s) 148, 429, 624
HpaII CCGG 3 cut(s) 110, 145, 632
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 2 cut(s) 136, 373
Hpy188III TCNNGA 2 cut(s) 145, 611
Hpy8I GTNNAC 1 cut(s) 162
HpyCH4III ACNGT 1 cut(s) 362
HpyCH4IV ACGT 1 cut(s) 581
HpyCH4V TGCA 5 cut(s) 331, 437, 452, 552, 592
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 337, 416, 518
HpyF3I CTNAG 3 cut(s) 17, 243, 531
HpySE526I ACGT 1 cut(s) 581
Hsp92II CATG 5 cut(s) 219, 269, 300, 331, 571
Kpn2I TCCGGA 1 cut(s) 144
KpnI GGTACC 1 cut(s) 366
KspI CCGCGG 1 cut(s) 158
Kzo9I GATC 1 cut(s) 558
Lsp1109I GCAGC 2 cut(s) 21, 241
MaeII ACGT 1 cut(s) 581
MaeIII GTNAC 2 cut(s) 523, 686
MalI GATC 1 cut(s) 560
MboI GATC 1 cut(s) 558
MboII GAAGA 1 cut(s) 568
MlsI TGGCCA 1 cut(s) 187
MluCI AATT 5 cut(s) 130, 193, 309, 332, 481
MluNI TGGCCA 1 cut(s) 187
MlyI GAGTC 1 cut(s) 633
MmeI TCCRAC 1 cut(s) 652
MnlI CCTC 4 cut(s) 191, 300, 471, 657
Mox20I TGGCCA 1 cut(s) 187
Mph1103I ATGCAT 1 cut(s) 439
MroI TCCGGA 1 cut(s) 144
MscI TGGCCA 1 cut(s) 187
MseI TTAA 2 cut(s) 308, 492
MslI CAYNNNNRTG 1 cut(s) 326
Msp20I TGGCCA 1 cut(s) 187
MspA1I CMGCKG 2 cut(s) 157, 242
MspI CCGG 3 cut(s) 110, 145, 632
MspR9I CCNGG 2 cut(s) 111, 477
MvaI CCWGG 1 cut(s) 477
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 4 cut(s) 68, 337, 416, 518
NciI CCSGG 1 cut(s) 111
NcoI CCATGG 2 cut(s) 265, 567
NdeII GATC 1 cut(s) 558
NlaIII CATG 5 cut(s) 219, 269, 300, 331, 571
NlaIV GGNNCC 5 cut(s) 204, 302, 364, 537, 636
NmuCI GTSAC 1 cut(s) 523
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 1 cut(s) 331
PfeI GAWTC 2 cut(s) 148, 429
PflMI CCANNNNNTGG 2 cut(s) 221, 266
PinAI ACCGGT 1 cut(s) 631
PkrI GCNGC 3 cut(s) 36, 70, 256
PleI GAGTC 1 cut(s) 632
PpsI GAGTC 1 cut(s) 632
Ppu21I YACGTR 1 cut(s) 582
PpuMI RGGWCCY 1 cut(s) 535
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 1 cut(s) 475
PspGI CCWGG 1 cut(s) 475
PspN4I GGNNCC 5 cut(s) 204, 302, 364, 537, 636
PspPI GGNCC 4 cut(s) 300, 535, 634, 668
PspPPI RGGWCCY 1 cut(s) 535
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 4 cut(s) 22, 80, 115, 364
RsaNI GTAC 4 cut(s) 21, 79, 114, 363
RseI CAYNNNNRTG 1 cut(s) 326
SacII CCGCGG 1 cut(s) 158
SaqAI TTAA 2 cut(s) 308, 492
SatI GCNGC 3 cut(s) 35, 69, 255
Sau3AI GATC 1 cut(s) 558
Sau96I GGNCC 4 cut(s) 300, 535, 634, 668
SchI GAGTC 1 cut(s) 633
ScrFI CCNGG 2 cut(s) 111, 477
SetI ASST 8 cut(s) 61, 208, 244, 368, 412, 499, 504, 584
Sfr303I CCGCGG 1 cut(s) 158
SgrBI CCGCGG 1 cut(s) 158
SinI GGWCC 3 cut(s) 535, 634, 668
SmiMI CAYNNNNRTG 1 cut(s) 326
SmlI CTYRAG 2 cut(s) 291, 383
SmoI CTYRAG 2 cut(s) 291, 383
Sse9I AATT 5 cut(s) 130, 193, 309, 332, 481
SsiI CCGC 3 cut(s) 69, 155, 157
StyD4I CCNGG 2 cut(s) 109, 475
StyI CCWWGG 2 cut(s) 265, 567
TaaI ACNGT 1 cut(s) 362
TaiI ACGT 1 cut(s) 584
TaqI TCGA 2 cut(s) 427, 649
TaqII GACCGA 1 cut(s) 359
TasI AATT 5 cut(s) 130, 193, 309, 332, 481
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 148, 429
Tru1I TTAA 2 cut(s) 308, 492
Tru9I TTAA 2 cut(s) 308, 492
TscAI CASTG 1 cut(s) 419
TseFI GTSAC 1 cut(s) 523
TseI GCWGC 2 cut(s) 34, 254
Tsp45I GTSAC 1 cut(s) 523
TspDTI ATGAA 1 cut(s) 42
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 1 cut(s) 419
Van91I CCANNNNNTGG 2 cut(s) 221, 266
VpaK11BI GGWCC 3 cut(s) 535, 634, 668
XapI RAATTY 1 cut(s) 130
XceI RCATGY 1 cut(s) 331
XcmI CCANNNNNNNNNTGG 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 161
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.