Rh2DG224000

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000345
Physical Location & Seq
Forward (+)
21668105 .. 21668608
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG224000.1

Sequence Viewer

Length: 345 bp
ATGTACACGATGACACCAGCTCTCAAGACGGATTTGAGAAACTCATGGCCGTCCGTGTTAAAAAAACACAGCAATATGTGGTTCTGGGAGCATGAGTACAAGAAACATGGCGCATGTACAGTAGAGTCAGGTGTACCACCTTTCACTCAAAAGTCCTACTTTGAGAAAGGGCACCAGCTATGGAACCAATATGACATCCATGCAGTGCTTAATCAAAGTGGTATTGTACCGGGTACTGCAACATCATATACTAGCATTGTAACCGCAATCCAAAAGAAAATCGGGAGTAACAATACTCCTTTAATCAAGTGCAGGACGATACACCTTGGTGACACACTTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

114

Amino Acids

12.98

Weight (kDa)

9.45

Isoelectric Point (pI)

27.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 7 - 106 8.7e-23 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 171
AciI CCGC 1 cut(s) 264
AcoI YGGCCR 1 cut(s) 47
AfaI GTAC 6 cut(s) 5, 98, 118, 135, 228, 235
AleI CACNNNNGTG 1 cut(s) 327
AluBI AGCT 2 cut(s) 20, 178
AluI AGCT 2 cut(s) 20, 178
AoxI GGCC 1 cut(s) 47
AspLEI GCGC 1 cut(s) 113
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 1 cut(s) 341
BaeGI GKGCMC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 171
BceAI ACGGC 1 cut(s) 34
BcnI CCSGG 1 cut(s) 231
BfaI CTAG 1 cut(s) 252
Bme1390I CCNGG 1 cut(s) 231
BmiI GGNNCC 2 cut(s) 173, 185
BmrFI CCNGG 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 231
BsaJI CCNNGG 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 1 cut(s) 195
BseSI GKGCMC 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 331
BshFI GGCC 1 cut(s) 49
BshNI GGYRCC 1 cut(s) 171
BsiSI CCGG 1 cut(s) 230
BsnI GGCC 1 cut(s) 49
Bsp1286I GDGCHC 1 cut(s) 174
Bsp1407I TGTACA 2 cut(s) 3, 116
BspACI CCGC 1 cut(s) 264
BspANI GGCC 1 cut(s) 49
BspLI GGNNCC 2 cut(s) 173, 185
BspT107I GGYRCC 1 cut(s) 171
BsrGI TGTACA 2 cut(s) 3, 116
BssECI CCNNGG 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 121
BstAUI TGTACA 2 cut(s) 3, 116
BstF5I GGATG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 113
BstNSI RCATGY 1 cut(s) 117
BstSCI CCNGG 1 cut(s) 229
BstSLI GKGCMC 1 cut(s) 174
BsuRI GGCC 1 cut(s) 49
BtsCI GGATG 1 cut(s) 195
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 1 cut(s) 210
CfoI GCGC 1 cut(s) 113
Csp6I GTAC 6 cut(s) 4, 97, 117, 134, 227, 234
CviAII CATG 5 cut(s) 45, 92, 107, 114, 200
CviJI RGCY 3 cut(s) 20, 49, 178
CviKI_1 RGCY 3 cut(s) 20, 49, 178
CviQI GTAC 6 cut(s) 4, 97, 117, 134, 227, 234
EaeI YGGCCR 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 5 cut(s) 48, 95, 110, 117, 203
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FatI CATG 5 cut(s) 44, 91, 106, 113, 199
FokI GGATG 1 cut(s) 182
FspBI CTAG 1 cut(s) 252
GlaI GCGC 1 cut(s) 112
HaeIII GGCC 1 cut(s) 49
HapII CCGG 1 cut(s) 230
HhaI GCGC 1 cut(s) 113
Hin1II CATG 5 cut(s) 48, 95, 110, 117, 203
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HinfI GANTC 1 cut(s) 125
HpaII CCGG 1 cut(s) 230
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 2 cut(s) 6, 134
Hpy188III TCNNGA 2 cut(s) 25, 283
Hpy8I GTNNAC 2 cut(s) 6, 134
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4V TGCA 3 cut(s) 203, 239, 312
Hsp92II CATG 5 cut(s) 48, 95, 110, 117, 203
HspAI GCGC 1 cut(s) 111
LmnI GCTCC 1 cut(s) 88
LpnPI CCDG 6 cut(s) 30, 70, 114, 188, 243, 298
MaeI CTAG 1 cut(s) 252
MaeIII GTNAC 3 cut(s) 259, 287, 329
MhlI GDGCHC 1 cut(s) 174
MlyI GAGTC 1 cut(s) 134
MseI TTAA 4 cut(s) 59, 210, 302, 339
MslI CAYNNNNRTG 1 cut(s) 327
MspI CCGG 1 cut(s) 230
MspR9I CCNGG 1 cut(s) 231
NciI CCSGG 1 cut(s) 231
NlaIII CATG 5 cut(s) 48, 95, 110, 117, 203
NlaIV GGNNCC 2 cut(s) 173, 185
NmuCI GTSAC 1 cut(s) 329
NspI RCATGY 1 cut(s) 117
OliI CACNNNNGTG 1 cut(s) 327
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 173, 185
RsaI GTAC 6 cut(s) 5, 98, 118, 135, 228, 235
RsaNI GTAC 6 cut(s) 4, 97, 117, 134, 227, 234
RseI CAYNNNNRTG 1 cut(s) 327
SaqAI TTAA 4 cut(s) 59, 210, 302, 339
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 1 cut(s) 174
SetI ASST 5 cut(s) 22, 133, 142, 180, 327
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
SsiI CCGC 1 cut(s) 264
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 1 cut(s) 229
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 1 cut(s) 121
TatI WGTACW 3 cut(s) 3, 96, 116
Tru1I TTAA 4 cut(s) 59, 210, 302, 339
Tru9I TTAA 4 cut(s) 59, 210, 302, 339
TscAI CASTG 1 cut(s) 210
TseFI GTSAC 1 cut(s) 329
Tsp45I GTSAC 1 cut(s) 329
TspGWI ACGGA 2 cut(s) 43, 44
TspRI CASTG 1 cut(s) 210
XceI RCATGY 1 cut(s) 117
XspI CTAG 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.