Rh2CG220000

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000345
Physical Location & Seq
Forward (+)
21926830 .. 21928592
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG220000.1

Sequence Viewer

Length: 369 bp
ATGCTGAACCATACGAGTACATGCAATTTGTACTCCAGTACCCACCTGGAGTTTGCTATGACAGATCAAAAGGTTATTGCATCTTACCTCTGCATGCCAACCAAGTTTCATGTACATGGAATATGGCCTTCCAACTTCTCCGATATAGAAGTCACTTGTGGTCAAGCACTGACGAATAACCCCTTTGATAAGGCGCAGAGTCAGCAAGGCTCTCAAGGAGCCGAATCGCACCGCAAGAACATCAAGCACAGCCTCATCGAGATCGCCGAGATCATGAAGACCAGATCGATGGCGAAGGATCTGAGCGGGATTGTGAAGGAGAATCGGCAAGGACCCCAAGGTTATTCAGTAAGAAATTACCGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

122

Amino Acids

13.79

Weight (kDa)

9.1

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 306
AciI CCGC 2 cut(s) 232, 306
AclWI GGATC 1 cut(s) 306
AfaI GTAC 4 cut(s) 19, 32, 40, 114
AjnI CCWGG 1 cut(s) 45
AlwI GGATC 1 cut(s) 306
AoxI GGCC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 1 cut(s) 332
AvaII GGWCC 1 cut(s) 332
BaeI ACNNNNGTAYC 2 cut(s) 22, 55
BbsI GAAGAC 1 cut(s) 284
BccI CCATC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 47
Bme1390I CCNGG 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 220, 334
BmrFI CCNGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 284
BpmI CTGGAG 2 cut(s) 19, 68
BpuEI CTTGAG 1 cut(s) 198
Bsa29I ATCGAT 1 cut(s) 287
BsaJI CCNNGG 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 36
BseBI CCWGG 1 cut(s) 47
BseCI ATCGAT 1 cut(s) 287
BseDI CCNNGG 1 cut(s) 337
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 36
BshFI GGCC 1 cut(s) 127
BshVI ATCGAT 1 cut(s) 287
BsnI GGCC 1 cut(s) 127
Bsp1407I TGTACA 1 cut(s) 112
Bsp143I GATC 5 cut(s) 64, 261, 270, 284, 298
BspACI CCGC 2 cut(s) 232, 306
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 294
BspDI ATCGAT 1 cut(s) 287
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 2 cut(s) 220, 334
BspPI GGATC 1 cut(s) 306
BsrBI CCGCTC 1 cut(s) 306
BsrGI TGTACA 1 cut(s) 112
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 1 cut(s) 337
BssMI GATC 5 cut(s) 64, 261, 270, 284, 298
BssT1I CCWWGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 47
Bst4CI ACNGT 1 cut(s) 366
BstAUI TGTACA 1 cut(s) 112
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 302
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 5 cut(s) 67, 264, 273, 287, 301
BstMBI GATC 5 cut(s) 64, 261, 270, 284, 298
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstNI CCWGG 1 cut(s) 47
BstNSI RCATGY 2 cut(s) 24, 97
BstSCI CCNGG 1 cut(s) 45
BstV2I GAAGAC 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 1 cut(s) 298
Bsu15I ATCGAT 1 cut(s) 287
BsuRI GGCC 1 cut(s) 127
BsuTUI ATCGAT 1 cut(s) 287
BtsIMutI CAGTG 1 cut(s) 167
Cac8I GCNNGC 1 cut(s) 95
CciI TCATGA 1 cut(s) 273
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 287
Csp6I GTAC 4 cut(s) 18, 31, 39, 113
CviAII CATG 5 cut(s) 21, 94, 110, 116, 274
CviJI RGCY 4 cut(s) 127, 210, 221, 252
CviKI_1 RGCY 4 cut(s) 127, 210, 221, 252
CviQI GTAC 4 cut(s) 18, 31, 39, 113
DdeI CTNAG 1 cut(s) 302
DpnI GATC 5 cut(s) 66, 263, 272, 286, 300
DpnII GATC 5 cut(s) 64, 261, 270, 284, 298
Eco130I CCWWGG 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 332
EcoO109I RGGNCCY 1 cut(s) 332
EcoRII CCWGG 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 5 cut(s) 24, 97, 113, 119, 277
FaiI YATR 9 cut(s) 12, 22, 59, 95, 111, 117, 124, 146, 275
FatI CATG 5 cut(s) 20, 93, 109, 115, 273
FauI CCCGC 1 cut(s) 299
GlaI GCGC 1 cut(s) 195
GsuI CTGGAG 2 cut(s) 19, 68
HaeIII GGCC 1 cut(s) 127
HhaI GCGC 1 cut(s) 196
Hin1II CATG 5 cut(s) 24, 97, 113, 119, 277
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HinfI GANTC 3 cut(s) 199, 224, 322
Hpy188I TCNGA 2 cut(s) 142, 303
Hpy188III TCNNGA 2 cut(s) 259, 274
Hpy99I CGWCG 1 cut(s) 366
HpyAV CCTTC 3 cut(s) 138, 289, 310
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4V TGCA 3 cut(s) 24, 80, 93
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 1 cut(s) 302
Hsp92II CATG 5 cut(s) 24, 97, 113, 119, 277
HspAI GCGC 1 cut(s) 194
Kzo9I GATC 5 cut(s) 64, 261, 270, 284, 298
LmnI GCTCC 1 cut(s) 218
LpnPI CCDG 4 cut(s) 32, 49, 59, 295
LweI GCATC 1 cut(s) 89
MaeIII GTNAC 1 cut(s) 151
MalI GATC 5 cut(s) 66, 263, 272, 286, 300
MbiI CCGCTC 1 cut(s) 306
MboI GATC 5 cut(s) 64, 261, 270, 284, 298
MboII GAAGA 1 cut(s) 289
MflI RGATCY 1 cut(s) 298
MluCI AATT 2 cut(s) 25, 355
MlyI GAGTC 1 cut(s) 208
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 2 cut(s) 98, 263
MslI CAYNNNNRTG 1 cut(s) 114
MspR9I CCNGG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 47
MwoI GCNNNNNNNGC 1 cut(s) 202
NdeII GATC 5 cut(s) 64, 261, 270, 284, 298
NlaIII CATG 5 cut(s) 24, 97, 113, 119, 277
NlaIV GGNNCC 2 cut(s) 220, 334
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 151
NspI RCATGY 2 cut(s) 24, 97
PaeI GCATGC 1 cut(s) 97
PagI TCATGA 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PfeI GAWTC 2 cut(s) 224, 322
PleI GAGTC 1 cut(s) 207
PpsI GAGTC 1 cut(s) 207
PpuMI RGGWCCY 1 cut(s) 332
Psp5II RGGWCCY 1 cut(s) 332
Psp6I CCWGG 1 cut(s) 45
PspGI CCWGG 1 cut(s) 45
PspN4I GGNNCC 2 cut(s) 220, 334
PspPI GGNCC 1 cut(s) 332
PspPPI RGGWCCY 1 cut(s) 332
PsuI RGATCY 1 cut(s) 298
RsaI GTAC 4 cut(s) 19, 32, 40, 114
RsaNI GTAC 4 cut(s) 18, 31, 39, 113
RseI CAYNNNNRTG 1 cut(s) 114
Sau3AI GATC 5 cut(s) 64, 261, 270, 284, 298
Sau96I GGNCC 1 cut(s) 332
SchI GAGTC 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 47
SetI ASST 4 cut(s) 48, 75, 90, 343
SfaNI GCATC 1 cut(s) 89
SinI GGWCC 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
SphI GCATGC 1 cut(s) 97
Sse9I AATT 2 cut(s) 25, 355
SsiI CCGC 2 cut(s) 232, 306
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 1 cut(s) 366
TaqI TCGA 2 cut(s) 258, 287
TasI AATT 2 cut(s) 25, 355
TatI WGTACW 3 cut(s) 17, 30, 112
TfiI GAWTC 2 cut(s) 224, 322
TscAI CASTG 1 cut(s) 174
TseFI GTSAC 1 cut(s) 151
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 2 cut(s) 98, 290
TspRI CASTG 1 cut(s) 174
VpaK11BI GGWCC 1 cut(s) 332
XceI RCATGY 2 cut(s) 24, 97
XcmI CCANNNNNNNNNTGG 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.