Rroxscaffold_2G00132220

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000345
Physical Location & Seq
Forward (+)
69226476 .. 69227166
691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00132220.1

Sequence Viewer

Length: 507 bp
ATGAAGAATTCAAACTTCATTATCCTTCTATCAGTATATGCACTCGTACAAGTTCTCAACGTTTCAAATGCTGAACCCTACGAGTACATGCAATTTGTACTCCAGTACCCACCTGGAGTTTGCTATGACAAATCAAAAGGTGATTGCATCTCACCTCTCCCAACCAAGTTTCATGTACATGGAATATGGCCTTCTAACTTCTCCAATATACTTGTCCGTTGTGGGCACGCACTGGCGAACAACCCCTTTCATAAGGCGCAGATGACACCTCTGCAGACGGATTTGGGAAACTCATGGCCGTCCGTGTTAACACAAAACAACAATATGCAGCTCTGGGAGCATGAGTATAGAGACCATGGTGCATGTGCAGTAGAGTCAGGTGTACCACCTTTCACTCAAAGGTCCTACTTTGAGAAAGGACACCAGCTATGGAACCAATATGACATCCTTGCAGTGCTTCATCAAAGTGGTATTAAACCGGGAGGACAGCCCTTGGTTACACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

168

Amino Acids

19.01

Weight (kDa)

6.74

Isoelectric Point (pI)

41.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 161 1.8e-28 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 60
AcoI YGGCCR 1 cut(s) 296
AcsI RAATTY 1 cut(s) 7
AfaI GTAC 6 cut(s) 48, 86, 99, 107, 177, 384
AgsI TTSAA 2 cut(s) 12, 66
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 2 cut(s) 331, 427
AluI AGCT 2 cut(s) 331, 427
Alw26I GTCTC 1 cut(s) 345
AoxI GGCC 2 cut(s) 188, 296
ApeKI GCWGC 1 cut(s) 328
ApoI RAATTY 1 cut(s) 7
AspLEI GCGC 1 cut(s) 259
AspS9I GGNCC 1 cut(s) 402
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 2 cut(s) 144, 152
AvaII GGWCC 1 cut(s) 402
BaeGI GKGCMC 1 cut(s) 228
BaeI ACNNNNGTAYC 2 cut(s) 89, 122
BbvI GCAGC 1 cut(s) 340
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 1 cut(s) 114
BcnI CCSGG 1 cut(s) 480
BcoDI GTCTC 1 cut(s) 345
BfmI CTRYAG 1 cut(s) 272
BisI GCNGC 1 cut(s) 329
BlsI GCNGC 1 cut(s) 330
Bme1390I CCNGG 2 cut(s) 114, 480
Bme18I GGWCC 1 cut(s) 402
BmgT120I GGNCC 1 cut(s) 402
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 2 cut(s) 114, 480
BmsI GCATC 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 86, 135
BpuMI CCSGG 1 cut(s) 480
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 2 cut(s) 355, 492
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bse1I ACTGG 2 cut(s) 103, 237
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 2 cut(s) 355, 492
BseGI GGATG 1 cut(s) 444
BseNI ACTGG 2 cut(s) 103, 237
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 387
BshFI GGCC 2 cut(s) 190, 298
BsiSI CCGG 1 cut(s) 479
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 2 cut(s) 190, 298
Bso31I GGTCTC 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 228
Bsp1407I TGTACA 1 cut(s) 175
Bsp19I CCATGG 1 cut(s) 355
BspANI GGCC 2 cut(s) 190, 298
BspLI GGNNCC 1 cut(s) 434
BspMAI CTGCAG 1 cut(s) 276
BspTNI GGTCTC 1 cut(s) 345
BsrGI TGTACA 1 cut(s) 175
BsrI ACTGG 2 cut(s) 103, 237
BssECI CCNNGG 2 cut(s) 355, 492
BssT1I CCWWGG 2 cut(s) 355, 492
Bst2UI CCWGG 1 cut(s) 114
BstAUI TGTACA 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 228
BstDSI CCRYGG 1 cut(s) 355
BstF5I GGATG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 259
BstMAI GTCTC 1 cut(s) 345
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstNI CCWGG 1 cut(s) 114
BstNSI RCATGY 2 cut(s) 91, 366
BstSCI CCNGG 2 cut(s) 112, 478
BstSFI CTRYAG 1 cut(s) 272
BstSLI GKGCMC 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 340
BsuRI GGCC 2 cut(s) 190, 298
BtgI CCRYGG 1 cut(s) 355
BtsCI GGATG 1 cut(s) 444
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 2 cut(s) 230, 459
Cac8I GCNNGC 1 cut(s) 228
CfoI GCGC 1 cut(s) 259
Cfr13I GGNCC 1 cut(s) 402
Csp6I GTAC 6 cut(s) 47, 85, 98, 106, 176, 383
CviAII CATG 7 cut(s) 88, 173, 179, 294, 341, 356, 363
CviJI RGCY 5 cut(s) 190, 298, 331, 427, 490
CviKI_1 RGCY 5 cut(s) 190, 298, 331, 427, 490
CviQI GTAC 6 cut(s) 47, 85, 98, 106, 176, 383
EaeI YGGCCR 1 cut(s) 296
Eco130I CCWWGG 2 cut(s) 355, 492
Eco31I GGTCTC 1 cut(s) 345
Eco47I GGWCC 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 112
EcoT14I CCWWGG 2 cut(s) 355, 492
ErhI CCWWGG 2 cut(s) 355, 492
FaeI CATG 7 cut(s) 91, 176, 182, 297, 344, 359, 366
FatI CATG 7 cut(s) 87, 172, 178, 293, 340, 355, 362
Fnu4HI GCNGC 1 cut(s) 329
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 1 cut(s) 329
GlaI GCGC 1 cut(s) 258
GluI GCNGC 1 cut(s) 329
GsuI CTGGAG 2 cut(s) 86, 135
HaeIII GGCC 2 cut(s) 190, 298
HapII CCGG 1 cut(s) 479
HhaI GCGC 1 cut(s) 259
Hin1II CATG 7 cut(s) 91, 176, 182, 297, 344, 359, 366
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 1 cut(s) 374
HpaI GTTAAC 1 cut(s) 309
HpaII CCGG 1 cut(s) 479
HphI GGTGA 2 cut(s) 144, 152
Hpy166II GTNNAC 2 cut(s) 309, 383
Hpy8I GTNNAC 2 cut(s) 309, 383
HpyAV CCTTC 2 cut(s) 35, 201
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 8 cut(s) 41, 91, 147, 274, 328, 362, 368, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 7 cut(s) 91, 176, 182, 297, 344, 359, 366
HspAI GCGC 1 cut(s) 257
KspAI GTTAAC 1 cut(s) 309
LmnI GCTCC 1 cut(s) 337
LpnPI CCDG 8 cut(s) 99, 116, 126, 218, 319, 363, 437, 492
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 1 cut(s) 156
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 496
MboII GAAGA 1 cut(s) 16
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 2 cut(s) 7, 92
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 3 cut(s) 165, 279, 476
MseI TTAA 2 cut(s) 308, 474
MslI CAYNNNNRTG 2 cut(s) 177, 465
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 2 cut(s) 114, 480
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 337
NciI CCSGG 1 cut(s) 480
NcoI CCATGG 1 cut(s) 355
NlaIII CATG 7 cut(s) 91, 176, 182, 297, 344, 359, 366
NlaIV GGNNCC 1 cut(s) 434
NspI RCATGY 2 cut(s) 91, 366
PkrI GCNGC 1 cut(s) 330
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 402
Psp1406I AACGTT 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspN4I GGNNCC 1 cut(s) 434
PspPI GGNCC 1 cut(s) 402
PspPPI RGGWCCY 1 cut(s) 402
PstI CTGCAG 1 cut(s) 276
RsaI GTAC 6 cut(s) 48, 86, 99, 107, 177, 384
RsaNI GTAC 6 cut(s) 47, 85, 98, 106, 176, 383
RseI CAYNNNNRTG 2 cut(s) 177, 465
SaqAI TTAA 2 cut(s) 308, 474
SatI GCNGC 1 cut(s) 329
Sau96I GGNCC 1 cut(s) 402
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 2 cut(s) 114, 480
SduI GDGCHC 1 cut(s) 228
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 1 cut(s) 272
SinI GGWCC 1 cut(s) 402
SmiMI CAYNNNNRTG 2 cut(s) 177, 465
Sse9I AATT 2 cut(s) 7, 92
StyD4I CCNGG 2 cut(s) 112, 478
StyI CCWWGG 2 cut(s) 355, 492
TaiI ACGT 1 cut(s) 63
TasI AATT 2 cut(s) 7, 92
TatI WGTACW 3 cut(s) 84, 97, 175
Tru1I TTAA 2 cut(s) 308, 474
Tru9I TTAA 2 cut(s) 308, 474
TscAI CASTG 2 cut(s) 237, 459
TseI GCWGC 1 cut(s) 328
TspDTI ATGAA 5 cut(s) 7, 17, 161, 239, 449
TspGWI ACGGA 3 cut(s) 206, 292, 293
TspRI CASTG 2 cut(s) 237, 459
VpaK11BI GGWCC 1 cut(s) 402
XapI RAATTY 1 cut(s) 7
XceI RCATGY 2 cut(s) 91, 366
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.