Rh3DG074900

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000345
Physical Location & Seq
Reverse (-)
5440747 .. 5453406
12660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG074900.1

Sequence Viewer

Length: 696 bp
ATGGTAGCAGTGACATCGTCGTTCTCTCTTCTTGTTCTTACTTTTGCTCTCTTCCAGTGTTTCTCTTTGGGCACCGCCAGCTACGATTATTTTCAATTTGTGCAGCAATGGCCACCACACTTCTGCTACGGTACCAATCGTCCTTGCATAAATAACAGGCAATCATTCACCATTCATGGCTTGTGGCCAAGTAATTTTTCACAAGTTCTTTACAACTATAATTGCGGTGGGGCCGCATTTAACAATGGGATGTTTGCAAATCAGACATTGGTAAACGACCTGACTCGCTCTTGGCCGGACCTGAAAAGAGGGAACAATAAAAGGTTTTGGGAAGAGCAGTGGGATAAGCATGGCAAATGCTCAGTACAGACGTATACACAAACGCAATACTTCCAGCGCTCCTTCCAGATGTGGAACCAATTCAATATTACTAGTATCTTTACTGCCGCTGGCGTCGTATCTCCGCCAGGGACACAACCAAGTAAAGTCAACGCCAGCGACTTAGAAGCACGCATTAAAGCAGTAACTCAGACGGCCCCTGTCCTCCGGTGCAGACAAGCTACAGTGCAGAACGTTAAACAGCCAGTTGAGTTGATTTATGAAGTGGGCATTTGTTACGATTATCTTGGAACAAATCTGATTCCTTGTACCAGCACACGAGGAAAATGCGGTGCAAATAATAATATTTACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

231

Amino Acids

26.28

Weight (kDa)

8.97

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 216 1.9e-38 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 131
AccB1I GGYRCC 2 cut(s) 71, 131
AccI GTMKAC 1 cut(s) 374
AciI CCGC 6 cut(s) 75, 225, 234, 447, 464, 669
AclI AACGTT 1 cut(s) 573
AcoI YGGCCR 3 cut(s) 110, 185, 293
AcyI GRCGYC 1 cut(s) 453
AfaI GTAC 3 cut(s) 133, 366, 649
AfeI AGCGCT 1 cut(s) 398
AgsI TTSAA 2 cut(s) 95, 424
AhlI ACTAGT 1 cut(s) 431
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 181, 213
AluBI AGCT 2 cut(s) 81, 560
AluI AGCT 2 cut(s) 81, 560
Aor51HI AGCGCT 1 cut(s) 398
AoxI GGCC 5 cut(s) 110, 185, 231, 293, 534
ApeKI GCWGC 1 cut(s) 103
Asp700I GAANNNNTTC 1 cut(s) 419
Asp718I GGTACC 1 cut(s) 131
AspLEI GCGC 1 cut(s) 399
AspS9I GGNCC 3 cut(s) 231, 298, 535
AsuHPI GGTGA 1 cut(s) 160
AvaII GGWCC 1 cut(s) 298
BaeGI GKGCMC 1 cut(s) 74
BalI TGGCCA 2 cut(s) 112, 187
BanI GGYRCC 2 cut(s) 71, 131
BauI CACGAG 1 cut(s) 657
BbvI GCAGC 1 cut(s) 115
BceAI ACGGC 1 cut(s) 549
BcgI CGANNNNNNTGC 3 cut(s) 31, 648, 682
BciT130I CCWGG 1 cut(s) 468
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 1 cut(s) 432
BfmI CTRYAG 1 cut(s) 561
BfoI RGCGCY 1 cut(s) 400
BisI GCNGC 3 cut(s) 104, 234, 447
BlsI GCNGC 3 cut(s) 105, 235, 448
Bme1390I CCNGG 1 cut(s) 468
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 3 cut(s) 231, 298, 535
BmiI GGNNCC 5 cut(s) 73, 133, 232, 416, 537
BmrFI CCNGG 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 467
BsaWI WCCGGW 1 cut(s) 546
Bse1I ACTGG 2 cut(s) 55, 584
Bse3DI GCAATG 1 cut(s) 113
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 1 cut(s) 255
BseMI GCAATG 1 cut(s) 113
BseMII CTCAG 2 cut(s) 375, 542
BseNI ACTGG 2 cut(s) 55, 584
BseSI GKGCMC 1 cut(s) 74
BseXI GCAGC 1 cut(s) 115
BsgI GTGCAG 3 cut(s) 122, 571, 587
BshFI GGCC 5 cut(s) 112, 187, 233, 295, 536
BshNI GGYRCC 2 cut(s) 71, 131
BsiSI CCGG 2 cut(s) 296, 547
BslFI GGGAC 1 cut(s) 484
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 5 cut(s) 112, 187, 233, 295, 536
Bsp1286I GDGCHC 1 cut(s) 74
BspACI CCGC 6 cut(s) 75, 225, 234, 447, 464, 669
BspANI GGCC 5 cut(s) 112, 187, 233, 295, 536
BspCNI CTCAG 2 cut(s) 374, 541
BspLI GGNNCC 5 cut(s) 73, 133, 232, 416, 537
BspQI GCTCTTC 1 cut(s) 327
BspT107I GGYRCC 2 cut(s) 71, 131
BsrDI GCAATG 1 cut(s) 113
BsrI ACTGG 2 cut(s) 55, 584
BssECI CCNNGG 1 cut(s) 467
BssNAI GTATAC 1 cut(s) 375
BssNI GRCGYC 1 cut(s) 453
BssSI CACGAG 1 cut(s) 657
Bst1107I GTATAC 1 cut(s) 375
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 2 cut(s) 131, 565
Bst6I CTCTTC 3 cut(s) 33, 56, 327
BstACI GRCGYC 1 cut(s) 453
BstC8I GCNNGC 4 cut(s) 79, 451, 496, 511
BstDEI CTNAG 3 cut(s) 361, 502, 528
BstF5I GGATG 1 cut(s) 255
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 1 cut(s) 399
BstMWI GCNNNNNNNGC 2 cut(s) 78, 109
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 1 cut(s) 466
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 115
BstZ17I GTATAC 1 cut(s) 375
BsuRI GGCC 5 cut(s) 112, 187, 233, 295, 536
BtsCI GGATG 1 cut(s) 255
BtsI GCAGTG 2 cut(s) 15, 344
BtsIMutI CAGTG 4 cut(s) 15, 62, 344, 570
Cac8I GCNNGC 4 cut(s) 79, 451, 496, 511
CfoI GCGC 1 cut(s) 399
Cfr13I GGNCC 3 cut(s) 231, 298, 535
CseI GACGC 1 cut(s) 442
Csp6I GTAC 3 cut(s) 132, 365, 648
CviAII CATG 2 cut(s) 176, 350
CviJI RGCY 9 cut(s) 81, 112, 180, 187, 233, 295, 536, 560, 583
CviKI_1 RGCY 9 cut(s) 81, 112, 180, 187, 233, 295, 536, 560, 583
CviQI GTAC 3 cut(s) 132, 365, 648
DdeI CTNAG 3 cut(s) 361, 502, 528
EaeI YGGCCR 3 cut(s) 110, 185, 293
Eam1104I CTCTTC 3 cut(s) 33, 56, 327
EarI CTCTTC 3 cut(s) 33, 56, 327
EciI GGCGGA 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 298
Eco47III AGCGCT 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 466
FaeI CATG 2 cut(s) 179, 353
FaiI YATR 6 cut(s) 149, 177, 219, 351, 375, 600
FaqI GGGAC 1 cut(s) 484
FatI CATG 2 cut(s) 175, 349
FblI GTMKAC 1 cut(s) 374
Fnu4HI GCNGC 3 cut(s) 104, 234, 447
FokI GGATG 1 cut(s) 262
Fsp4HI GCNGC 3 cut(s) 104, 234, 447
FspBI CTAG 1 cut(s) 432
GlaI GCGC 1 cut(s) 398
GluI GCNGC 3 cut(s) 104, 234, 447
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 5 cut(s) 112, 187, 233, 295, 536
HapII CCGG 2 cut(s) 296, 547
HgaI GACGC 1 cut(s) 442
HhaI GCGC 1 cut(s) 399
Hin1I GRCGYC 1 cut(s) 453
Hin1II CATG 2 cut(s) 179, 353
Hin6I GCGC 1 cut(s) 397
HinP1I GCGC 1 cut(s) 397
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HinfI GANTC 2 cut(s) 283, 640
HpaII CCGG 2 cut(s) 296, 547
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 3 cut(s) 274, 375, 490
Hpy188I TCNGA 3 cut(s) 264, 531, 639
Hpy188III TCNNGA 1 cut(s) 406
Hpy8I GTNNAC 3 cut(s) 274, 375, 490
Hpy99I CGWCG 2 cut(s) 22, 458
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 2 cut(s) 131, 565
HpyCH4IV ACGT 2 cut(s) 371, 573
HpyCH4V TGCA 6 cut(s) 103, 147, 257, 552, 568, 674
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 109
HpyF3I CTNAG 3 cut(s) 361, 502, 528
HpySE526I ACGT 2 cut(s) 371, 573
Hsp92I GRCGYC 1 cut(s) 453
Hsp92II CATG 2 cut(s) 179, 353
HspAI GCGC 1 cut(s) 397
KpnI GGTACC 1 cut(s) 135
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 1 cut(s) 404
Lsp1109I GCAGC 1 cut(s) 115
MaeI CTAG 1 cut(s) 432
MaeII ACGT 2 cut(s) 371, 573
MaeIII GTNAC 3 cut(s) 10, 523, 614
MboII GAAGA 3 cut(s) 20, 43, 344
MhlI GDGCHC 1 cut(s) 74
MlsI TGGCCA 2 cut(s) 112, 187
MluCI AATT 4 cut(s) 95, 193, 220, 419
MluNI TGGCCA 2 cut(s) 112, 187
MlyI GAGTC 1 cut(s) 277
MnlI CCTC 3 cut(s) 302, 554, 653
Mox20I TGGCCA 2 cut(s) 112, 187
MroXI GAANNNNTTC 1 cut(s) 419
MscI TGGCCA 2 cut(s) 112, 187
MseI TTAA 4 cut(s) 240, 516, 576, 694
Msp20I TGGCCA 2 cut(s) 112, 187
MspA1I CMGCKG 1 cut(s) 449
MspI CCGG 2 cut(s) 296, 547
MspR9I CCNGG 1 cut(s) 468
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 78, 109
NlaIII CATG 2 cut(s) 179, 353
NlaIV GGNNCC 5 cut(s) 73, 133, 232, 416, 537
NmuCI GTSAC 1 cut(s) 10
PciSI GCTCTTC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 419
PfeI GAWTC 1 cut(s) 640
PflFI GACNNNGTC 1 cut(s) 16
PkrI GCNGC 3 cut(s) 105, 235, 448
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp1406I AACGTT 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 5 cut(s) 73, 133, 232, 416, 537
PspPI GGNCC 3 cut(s) 231, 298, 535
PsyI GACNNNGTC 1 cut(s) 16
RsaI GTAC 3 cut(s) 133, 366, 649
RsaNI GTAC 3 cut(s) 132, 365, 648
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 4 cut(s) 240, 516, 576, 694
SatI GCNGC 3 cut(s) 104, 234, 447
Sau96I GGNCC 3 cut(s) 231, 298, 535
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 1 cut(s) 74
SetI ASST 7 cut(s) 83, 282, 303, 326, 374, 562, 576
SfcI CTRYAG 1 cut(s) 561
SinI GGWCC 1 cut(s) 298
SpeI ACTAGT 1 cut(s) 431
Sse9I AATT 4 cut(s) 95, 193, 220, 419
SsiI CCGC 6 cut(s) 75, 225, 234, 447, 464, 669
SspI AATATT 2 cut(s) 427, 685
SspMI CTAG 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 466
TaaI ACNGT 2 cut(s) 131, 565
TaiI ACGT 2 cut(s) 374, 576
TasI AATT 4 cut(s) 95, 193, 220, 419
TatI WGTACW 1 cut(s) 364
TauI GCSGC 2 cut(s) 236, 449
TfiI GAWTC 1 cut(s) 640
Tru1I TTAA 4 cut(s) 240, 516, 576, 694
Tru9I TTAA 4 cut(s) 240, 516, 576, 694
TscAI CASTG 4 cut(s) 15, 62, 344, 570
TseFI GTSAC 1 cut(s) 10
TseI GCWGC 1 cut(s) 103
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 2 cut(s) 164, 615
TspRI CASTG 4 cut(s) 15, 62, 344, 570
Tth111I GACNNNGTC 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 374
XmnI GAANNNNTTC 1 cut(s) 419
XspI CTAG 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.