Rorug03G0029200

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000345
Physical Location & Seq
Forward (+)
2343704 .. 2345126
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0029200.1

Sequence Viewer

Length: 546 bp
ATGGCTTTCCAAGCAGAGGATCTCAACCTTGAAGCCACCGAGCTCAGATTAGGCCTACCCGGAACCAAGGAATCCGAGCAACAAAAAACGAACAACAAAAGAGCATTGCCAGTTGAGCCTGAATCCAATGCTTCTTCAGCTCCCAAGCCTCCTCCCTCCAAGGCACAAGTAGTTGGATGGCCACCGGTGAGATCATACCGGAAAAACTGTTTCCAGGCGAAGAAAACCGAGGGAGAAAGCGGTGGGATGTTCGTGAAAGTCAGCATGGATGGAGCTCCTTACCTTCGAAAGATGGACTTGAAGGTTTACAAAGGATATCCAGAGCTGCTTAAGGCCTTGGAGGACATGTTCAAGTTCAAGGTTGGTGAATATTGTGAGAAGGAACTAGGGTACAACAACAGATCCGAGTTTGTGCCAACTTATGAAGACAAAGATGGAGATTGGATGCTTCTTGGAGATGTTCCATGGGAGATGTTTTTCACTTCATGCAAGAGGCTGAGAATTATGAAAGGTTCAGAAGCCAAAGGCTTAGGTTGTGCGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

181

Amino Acids

20.41

Weight (kDa)

8.24

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AUX_IAA PF02309 28 - 178 6.7e-56 AUX/IAA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 240, 539
AclWI GGATC 2 cut(s) 27, 396
AcoI YGGCCR 1 cut(s) 179
AcuI CTGAAG 1 cut(s) 120
AfaI GTAC 1 cut(s) 392
AfiI CCNNNNNNNGG 1 cut(s) 16
AflII CTTAAG 1 cut(s) 329
AflIII ACRYGT 1 cut(s) 345
AgeI ACCGGT 1 cut(s) 184
AgsI TTSAA 4 cut(s) 32, 301, 352, 358
AjnI CCWGG 1 cut(s) 213
AloI GAACNNNNNNTCC 2 cut(s) 332, 364
AluBI AGCT 4 cut(s) 43, 140, 275, 325
AluI AGCT 4 cut(s) 43, 140, 275, 325
Alw21I GWGCWC 2 cut(s) 45, 277
AlwI GGATC 2 cut(s) 27, 396
AoxI GGCC 3 cut(s) 52, 179, 333
ApeKI GCWGC 1 cut(s) 325
AsiGI ACCGGT 1 cut(s) 184
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 2 cut(s) 199, 377
AsuII TTCGAA 1 cut(s) 286
BalI TGGCCA 1 cut(s) 181
BanII GRGCYC 2 cut(s) 45, 277
BbsI GAAGAC 1 cut(s) 432
Bbv12I GWGCWC 2 cut(s) 45, 277
BbvI GCAGC 1 cut(s) 312
BccI CCATC 4 cut(s) 171, 263, 286, 428
BciT130I CCWGG 1 cut(s) 215
BcnI CCSGG 1 cut(s) 60
BfaI CTAG 1 cut(s) 386
BfrI CTTAAG 1 cut(s) 329
BisI GCNGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 327
Bme1390I CCNGG 2 cut(s) 60, 215
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 2 cut(s) 60, 215
BmsI GCATC 1 cut(s) 435
BpiI GAAGAC 1 cut(s) 432
Bpu10I CCTNAGC 1 cut(s) 529
Bpu14I TTCGAA 1 cut(s) 286
BpuMI CCSGG 1 cut(s) 60
BsaJI CCNNGG 5 cut(s) 66, 159, 228, 336, 464
BsaWI WCCGGW 2 cut(s) 184, 198
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 104
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 5 cut(s) 66, 159, 228, 336, 464
BseGI GGATG 4 cut(s) 182, 252, 274, 450
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMI GCAATG 1 cut(s) 104
BseMII CTCAG 2 cut(s) 58, 488
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 1 cut(s) 141
BseXI GCAGC 1 cut(s) 312
BshFI GGCC 3 cut(s) 54, 181, 335
BshTI ACCGGT 1 cut(s) 184
BsiHKAI GWGCWC 2 cut(s) 45, 277
BsiSI CCGG 3 cut(s) 60, 185, 199
BslI CCNNNNNNNGG 1 cut(s) 16
BsnI GGCC 3 cut(s) 54, 181, 335
Bsp119I TTCGAA 1 cut(s) 286
Bsp1286I GDGCHC 2 cut(s) 45, 277
Bsp143I GATC 3 cut(s) 19, 191, 401
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 2 cut(s) 240, 539
BspANI GGCC 3 cut(s) 54, 181, 335
BspCNI CTCAG 2 cut(s) 57, 489
BspLI GGNNCC 1 cut(s) 64
BspPI GGATC 2 cut(s) 27, 396
BspT104I TTCGAA 1 cut(s) 286
BspTI CTTAAG 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 104
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 1 cut(s) 110
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 5 cut(s) 66, 159, 228, 336, 464
BssMI GATC 3 cut(s) 19, 191, 401
BssT1I CCWWGG 4 cut(s) 66, 159, 336, 464
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 209
BstAFI CTTAAG 1 cut(s) 329
BstBI TTCGAA 1 cut(s) 286
BstDEI CTNAG 3 cut(s) 44, 497, 529
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 4 cut(s) 182, 252, 274, 450
BstKTI GATC 3 cut(s) 22, 194, 404
BstMBI GATC 3 cut(s) 19, 191, 401
BstMWI GCNNNNNNNGC 3 cut(s) 11, 115, 137
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 2 cut(s) 58, 213
BstV1I GCAGC 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 2 cut(s) 19, 401
BstYI RGATCY 2 cut(s) 19, 401
BsuRI GGCC 3 cut(s) 54, 181, 335
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 4 cut(s) 182, 252, 274, 450
Cfr10I RCCGGY 1 cut(s) 184
Csp6I GTAC 1 cut(s) 391
CspAI ACCGGT 1 cut(s) 184
CviAII CATG 4 cut(s) 265, 346, 465, 486
CviQI GTAC 1 cut(s) 391
DdeI CTNAG 3 cut(s) 44, 497, 529
DpnI GATC 3 cut(s) 21, 193, 403
DpnII GATC 3 cut(s) 19, 191, 401
EaeI YGGCCR 1 cut(s) 179
Ecl136II GAGCTC 2 cut(s) 43, 275
Eco130I CCWWGG 4 cut(s) 66, 159, 336, 464
Eco147I AGGCCT 2 cut(s) 54, 335
Eco24I GRGCYC 2 cut(s) 45, 277
Eco32I GATATC 1 cut(s) 317
Eco53kI GAGCTC 2 cut(s) 43, 275
Eco57I CTGAAG 1 cut(s) 120
EcoICRI GAGCTC 2 cut(s) 43, 275
EcoRII CCWGG 1 cut(s) 213
EcoRV GATATC 1 cut(s) 317
EcoT14I CCWWGG 4 cut(s) 66, 159, 336, 464
EcoT38I GRGCYC 2 cut(s) 45, 277
ErhI CCWWGG 4 cut(s) 66, 159, 336, 464
FaeI CATG 4 cut(s) 268, 349, 468, 489
FaiI YATR 7 cut(s) 196, 266, 347, 423, 466, 487, 506
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FatI CATG 4 cut(s) 264, 345, 464, 485
Fnu4HI GCNGC 1 cut(s) 326
FokI GGATG 4 cut(s) 189, 259, 281, 457
FriOI GRGCYC 2 cut(s) 45, 277
Fsp4HI GCNGC 1 cut(s) 326
FspBI CTAG 1 cut(s) 386
GluI GCNGC 1 cut(s) 326
HaeIII GGCC 3 cut(s) 54, 181, 335
HapII CCGG 3 cut(s) 60, 185, 199
Hin1II CATG 4 cut(s) 268, 349, 468, 489
HinfI GANTC 2 cut(s) 71, 122
HpaII CCGG 3 cut(s) 60, 185, 199
HphI GGTGA 2 cut(s) 199, 377
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 4 cut(s) 47, 76, 406, 517
Hpy188III TCNNGA 2 cut(s) 253, 320
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 3 cut(s) 293, 295, 373
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4V TGCA 1 cut(s) 489
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 115, 137
HpyF3I CTNAG 3 cut(s) 44, 497, 529
Hsp92II CATG 4 cut(s) 268, 349, 468, 489
Kzo9I GATC 3 cut(s) 19, 191, 401
LmnI GCTCC 3 cut(s) 145, 272, 280
LpnPI CCDG 8 cut(s) 73, 123, 132, 198, 200, 212, 227, 333
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 1 cut(s) 435
MaeI CTAG 1 cut(s) 386
MalI GATC 3 cut(s) 21, 193, 403
MboI GATC 3 cut(s) 19, 191, 401
MboII GAAGA 3 cut(s) 126, 232, 437
MflI RGATCY 2 cut(s) 19, 401
MhlI GDGCHC 2 cut(s) 45, 277
MlsI TGGCCA 1 cut(s) 181
MluCI AATT 1 cut(s) 501
MluNI TGGCCA 1 cut(s) 181
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 7 cut(s) 10, 159, 162, 166, 223, 334, 486
Mox20I TGGCCA 1 cut(s) 181
MscI TGGCCA 1 cut(s) 181
MseI TTAA 1 cut(s) 330
Msp20I TGGCCA 1 cut(s) 181
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 3 cut(s) 60, 185, 199
MspR9I CCNGG 2 cut(s) 60, 215
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 3 cut(s) 11, 115, 137
NciI CCSGG 1 cut(s) 60
NcoI CCATGG 1 cut(s) 464
NdeII GATC 3 cut(s) 19, 191, 401
NlaIII CATG 4 cut(s) 268, 349, 468, 489
NlaIV GGNNCC 1 cut(s) 64
NspI RCATGY 1 cut(s) 349
NspV TTCGAA 1 cut(s) 286
PceI AGGCCT 2 cut(s) 54, 335
PciI ACATGT 1 cut(s) 345
PfeI GAWTC 2 cut(s) 71, 122
PinAI ACCGGT 1 cut(s) 184
PkrI GCNGC 1 cut(s) 327
PscI ACATGT 1 cut(s) 345
Psp124BI GAGCTC 2 cut(s) 45, 277
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 64
PsuI RGATCY 2 cut(s) 19, 401
RsaI GTAC 1 cut(s) 392
RsaNI GTAC 1 cut(s) 391
SacI GAGCTC 2 cut(s) 45, 277
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 1 cut(s) 326
Sau3AI GATC 3 cut(s) 19, 191, 401
ScrFI CCNGG 2 cut(s) 60, 215
SduI GDGCHC 2 cut(s) 45, 277
SfaNI GCATC 1 cut(s) 435
SfuI TTCGAA 1 cut(s) 286
SgrAI CRCCGGYG 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 1 cut(s) 501
SseBI AGGCCT 2 cut(s) 54, 335
SsiI CCGC 2 cut(s) 240, 539
SspI AATATT 1 cut(s) 371
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 2 cut(s) 45, 277
StuI AGGCCT 2 cut(s) 54, 335
StyD4I CCNGG 2 cut(s) 58, 213
StyI CCWWGG 4 cut(s) 66, 159, 336, 464
TaaI ACNGT 1 cut(s) 209
TaqI TCGA 1 cut(s) 286
TasI AATT 1 cut(s) 501
TfiI GAWTC 2 cut(s) 71, 122
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TseI GCWGC 1 cut(s) 325
TspDTI ATGAA 3 cut(s) 438, 474, 521
Vha464I CTTAAG 1 cut(s) 329
XceI RCATGY 1 cut(s) 349
XspI CTAG 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.