Rh3AG081000

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000345
Physical Location & Seq
Forward (+)
6492356 .. 6493160
805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG081000.1

Sequence Viewer

Length: 705 bp
ATGGAAACGGCATTGCTATGTACAGTCTCCATGCTGGTTATTTCTGCTTTTCCATCCGGTGCTAAAGCCGCGAATCAGTACGACTATCTTCAATTTGTTCAACAATGGCCGATAACCTTGTGCTACAACAACCCAGCCTGTATTCCAGGAGCATCACTCCCACTGGACTTCTTTATCCATGGGTTATGGCCAAGCAATTTCTCTGGCCAGAATCAGCCCTGTGTTGGTACTCCGTTCGACTATAATGAGATGCTTAGTCAATATCGTCTCCGAACCAAAATGCTACCATTCTCATGGCCAAGCTTCACTCGTAGATCCAACATGGGCTTCTGGAAATATGAGTATAATCAACACGGCACTTGTTCGGAGAATAACCTCGCGCAGACGGACTATTTTTACAAAGCCTATGCTCTCTGGATGCGCTACAATGCAAACTTTATATTATATGCATCTACAGTTATACCAGGCCACAAGATTGTGCCGGGATACCTCTACCATTACGCTGACCTTCTAAACTCCGTTCAACAAGCAATTGGTGGATTCACTCCTTCGCTTATGTGCAAGCACGAGCCTGCAAATAATACTTGGATTCTGCACGAAGTCATCATATGTTTTGATGCCATGGGGAACAACGTCATCAATTGTGTTAGAGGATCTAGTTGCTCTGCCACTACCACCGGGTATATACACTATCCTTTGCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

234

Amino Acids

26.61

Weight (kDa)

7.01

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 215 3.7e-34 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 71, 380
AciI CCGC 1 cut(s) 69
AclWI GGATC 2 cut(s) 309, 661
AcoI YGGCCR 4 cut(s) 107, 188, 205, 296
AfaI GTAC 3 cut(s) 22, 80, 229
AfiI CCNNNNNNNGG 1 cut(s) 224
AgsI TTSAA 3 cut(s) 92, 101, 524
AjnI CCWGG 2 cut(s) 145, 463
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 1 cut(s) 303
AluI AGCT 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 31, 272
AlwI GGATC 2 cut(s) 309, 661
AoxI GGCC 5 cut(s) 107, 188, 205, 296, 466
AspLEI GCGC 2 cut(s) 382, 423
AsuC2I CCSGG 2 cut(s) 483, 679
BalI TGGCCA 3 cut(s) 190, 207, 298
BauI CACGAG 1 cut(s) 566
BccI CCATC 1 cut(s) 61
BceAI ACGGC 2 cut(s) 24, 370
BciT130I CCWGG 2 cut(s) 147, 465
BciVI GTATCC 1 cut(s) 479
BcnI CCSGG 2 cut(s) 483, 679
BcoDI GTCTC 2 cut(s) 31, 272
BfaI CTAG 1 cut(s) 657
BfmI CTRYAG 1 cut(s) 453
BfuI GTATCC 1 cut(s) 479
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 4 cut(s) 147, 465, 483, 679
BmrFI CCNGG 4 cut(s) 147, 465, 483, 679
BmsI GCATC 5 cut(s) 161, 240, 408, 458, 607
BplI GAGNNNNNCTC 2 cut(s) 141, 173
BpuMI CCSGG 2 cut(s) 483, 679
BsaJI CCNNGG 2 cut(s) 178, 621
BsaWI WCCGGW 1 cut(s) 56
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse1I ACTGG 1 cut(s) 168
Bse3DI GCAATG 1 cut(s) 11
BseBI CCWGG 2 cut(s) 147, 465
BseDI CCNNGG 2 cut(s) 178, 621
BseGI GGATG 2 cut(s) 53, 423
BseLI CCNNNNNNNGG 1 cut(s) 224
BseMI GCAATG 1 cut(s) 11
BseNI ACTGG 1 cut(s) 168
BseYI CCCAGC 1 cut(s) 133
BsgI GTGCAG 1 cut(s) 578
Bsh1236I CGCG 2 cut(s) 71, 380
BshFI GGCC 5 cut(s) 109, 190, 207, 298, 468
BsiSI CCGG 3 cut(s) 57, 482, 678
BslI CCNNNNNNNGG 1 cut(s) 224
BsmAI GTCTC 2 cut(s) 31, 272
BsmBI CGTCTC 1 cut(s) 272
BsnI GGCC 5 cut(s) 109, 190, 207, 298, 468
Bsp1407I TGTACA 1 cut(s) 20
Bsp143I GATC 2 cut(s) 314, 653
Bsp19I CCATGG 2 cut(s) 178, 621
BspACI CCGC 1 cut(s) 69
BspANI GGCC 5 cut(s) 109, 190, 207, 298, 468
BspFNI CGCG 2 cut(s) 71, 380
BspPI GGATC 2 cut(s) 309, 661
BsrDI GCAATG 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 20
BsrI ACTGG 1 cut(s) 168
BssECI CCNNGG 2 cut(s) 178, 621
BssMI GATC 2 cut(s) 314, 653
BssSI CACGAG 1 cut(s) 566
BssT1I CCWWGG 2 cut(s) 178, 621
Bst2BI CACGAG 1 cut(s) 566
Bst2UI CCWGG 2 cut(s) 147, 465
Bst4CI ACNGT 2 cut(s) 25, 457
BstAUI TGTACA 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 563, 573
BstDEI CTNAG 1 cut(s) 254
BstDSI CCRYGG 2 cut(s) 178, 621
BstF5I GGATG 2 cut(s) 53, 423
BstFNI CGCG 2 cut(s) 71, 380
BstHHI GCGC 2 cut(s) 382, 423
BstKTI GATC 2 cut(s) 317, 656
BstMAI GTCTC 2 cut(s) 31, 272
BstMBI GATC 2 cut(s) 314, 653
BstMWI GCNNNNNNNGC 1 cut(s) 68
BstNI CCWGG 2 cut(s) 147, 465
BstSCI CCNGG 4 cut(s) 145, 463, 481, 677
BstSFI CTRYAG 1 cut(s) 453
BstUI CGCG 2 cut(s) 71, 380
BstX2I RGATCY 2 cut(s) 314, 653
BstXI CCANNNNNNTGG 1 cut(s) 294
BstYI RGATCY 2 cut(s) 314, 653
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 5 cut(s) 109, 190, 207, 298, 468
BtgI CCRYGG 2 cut(s) 178, 621
BtsCI GGATG 2 cut(s) 53, 423
BtsIMutI CAGTG 1 cut(s) 161
Cac8I GCNNGC 2 cut(s) 563, 573
CfoI GCGC 2 cut(s) 382, 423
Csp6I GTAC 3 cut(s) 21, 79, 228
CviAII CATG 5 cut(s) 31, 179, 294, 322, 622
CviQI GTAC 3 cut(s) 21, 79, 228
DdeI CTNAG 1 cut(s) 254
DpnI GATC 2 cut(s) 316, 655
DpnII GATC 2 cut(s) 314, 653
EaeI YGGCCR 4 cut(s) 107, 188, 205, 296
Eco130I CCWWGG 2 cut(s) 178, 621
EcoRII CCWGG 2 cut(s) 145, 463
EcoT14I CCWWGG 2 cut(s) 178, 621
EcoT22I ATGCAT 1 cut(s) 451
ErhI CCWWGG 2 cut(s) 178, 621
Esp3I CGTCTC 1 cut(s) 272
FaeI CATG 5 cut(s) 34, 182, 297, 325, 625
FatI CATG 5 cut(s) 30, 178, 293, 321, 621
FauNDI CATATG 1 cut(s) 608
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 2 cut(s) 40, 430
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 1 cut(s) 657
GlaI GCGC 2 cut(s) 381, 422
GluI GCNGC 1 cut(s) 69
GsaI CCCAGC 1 cut(s) 137
HaeIII GGCC 5 cut(s) 109, 190, 207, 298, 468
HapII CCGG 3 cut(s) 57, 482, 678
HhaI GCGC 2 cut(s) 382, 423
Hin1II CATG 5 cut(s) 34, 182, 297, 325, 625
Hin6I GCGC 2 cut(s) 380, 421
HinP1I GCGC 2 cut(s) 380, 421
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 4 cut(s) 73, 211, 540, 589
HpaII CCGG 3 cut(s) 57, 482, 678
Hpy188I TCNGA 2 cut(s) 272, 367
Hpy188III TCNNGA 2 cut(s) 331, 415
HpyAV CCTTC 2 cut(s) 518, 558
HpyCH4III ACNGT 2 cut(s) 25, 457
HpyCH4IV ACGT 1 cut(s) 633
HpyCH4V TGCA 6 cut(s) 431, 449, 561, 575, 595, 700
HpyF10VI GCNNNNNNNGC 1 cut(s) 68
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 1 cut(s) 633
Hsp92II CATG 5 cut(s) 34, 182, 297, 325, 625
HspAI GCGC 2 cut(s) 380, 421
Kzo9I GATC 2 cut(s) 314, 653
LmnI GCTCC 1 cut(s) 149
LweI GCATC 5 cut(s) 161, 240, 408, 458, 607
MaeI CTAG 1 cut(s) 657
MaeII ACGT 1 cut(s) 633
MalI GATC 2 cut(s) 316, 655
MboI GATC 2 cut(s) 314, 653
MboII GAAGA 1 cut(s) 80
MfeI CAATTG 2 cut(s) 531, 640
MflI RGATCY 2 cut(s) 314, 653
MlsI TGGCCA 3 cut(s) 190, 207, 298
MluCI AATT 4 cut(s) 92, 196, 531, 640
MluNI TGGCCA 3 cut(s) 190, 207, 298
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 3 cut(s) 386, 500, 644
Mox20I TGGCCA 3 cut(s) 190, 207, 298
Mph1103I ATGCAT 1 cut(s) 451
MscI TGGCCA 3 cut(s) 190, 207, 298
MslI CAYNNNNRTG 2 cut(s) 16, 292
Msp20I TGGCCA 3 cut(s) 190, 207, 298
MspI CCGG 3 cut(s) 57, 482, 678
MspR9I CCNGG 4 cut(s) 147, 465, 483, 679
MunI CAATTG 2 cut(s) 531, 640
MvaI CCWGG 2 cut(s) 147, 465
MvnI CGCG 2 cut(s) 71, 380
MwoI GCNNNNNNNGC 1 cut(s) 68
NciI CCSGG 2 cut(s) 483, 679
NcoI CCATGG 2 cut(s) 178, 621
NdeI CATATG 1 cut(s) 608
NdeII GATC 2 cut(s) 314, 653
NlaIII CATG 5 cut(s) 34, 182, 297, 325, 625
NsiI ATGCAT 1 cut(s) 451
PfeI GAWTC 4 cut(s) 73, 211, 540, 589
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 1 cut(s) 70
Psp6I CCWGG 2 cut(s) 145, 463
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 2 cut(s) 145, 463
PsuI RGATCY 2 cut(s) 314, 653
RsaI GTAC 3 cut(s) 22, 80, 229
RsaNI GTAC 3 cut(s) 21, 79, 228
RseI CAYNNNNRTG 2 cut(s) 16, 292
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 2 cut(s) 314, 653
ScrFI CCNGG 4 cut(s) 147, 465, 483, 679
SetI ASST 6 cut(s) 119, 305, 378, 492, 510, 636
SfaNI GCATC 5 cut(s) 161, 240, 408, 458, 607
SfcI CTRYAG 1 cut(s) 453
SmiMI CAYNNNNRTG 2 cut(s) 16, 292
Sse9I AATT 4 cut(s) 92, 196, 531, 640
SsiI CCGC 1 cut(s) 69
SspMI CTAG 1 cut(s) 657
StyD4I CCNGG 4 cut(s) 145, 463, 481, 677
StyI CCWWGG 2 cut(s) 178, 621
TaaI ACNGT 2 cut(s) 25, 457
TaiI ACGT 1 cut(s) 636
TaqI TCGA 1 cut(s) 237
TasI AATT 4 cut(s) 92, 196, 531, 640
TatI WGTACW 1 cut(s) 20
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 4 cut(s) 73, 211, 540, 589
TscAI CASTG 1 cut(s) 168
TspGWI ACGGA 3 cut(s) 222, 401, 508
TspRI CASTG 1 cut(s) 168
XspI CTAG 1 cut(s) 657
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.