RLG00000025197

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000345
Physical Location & Seq
Reverse (-)
42945728 .. 42946509
782 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025197

Sequence Viewer

Length: 693 bp
ATGGGGATCATCCTACTCGTCCTGCTTCTTCTCTCTGCGTGGGTGGCAGCTGATGCAGCCAATCCCTATGACTACTTCCAGTTTGTGCAGCAATGGCCGGCTGAATTCTGTTATGGGAATCCACGTTGTATCCTACAAGTCCCGCAGCACTTTACGGTGCACGGCCTTTGGCCATCCAATAACTCATGGACTTCTCCACAGGATGCCGTCTGCGTTGGCACACAATTTAATGAAATTGAGATGGCCCAAAACGGTCAATTGGAGAACAATCTTCGAAGAGCATGGCCCCAATTGATAGCTTATTTTACCGATATGGACTTCTGGGGAGATGAATACAATAAGCACGGTACTTGCTCGGAGAACAAGCTGGACCAGACGCAATATTTTCGGAAAGCTTACCGGATGTGGTCTCTGTACAGAGCAGATGAGCTGTTTCAAAACTCAGGAATCTACGGTGGACGTTACCATACTCGCACTGCCCTTGAAAATGCCATTTGGCGAGGGACTAGAGGCTGGCCTCTGCTTAGCTGCTGGTCCAGGAACATAAAAGTAGCAGGTGCCAAATATGAAAATGTTTGGTTTTTGGAGGAGGTCATCATTTGTTTTGATTATCTGGGAGAGAACGTCATCGATTGTGTTCGACGGACAAATTGCGGTGTAACAATATATTATCGTCGTTACCAATGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

231

Amino Acids

27.08

Weight (kDa)

6.09

Isoelectric Point (pI)

29.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 23 - 212 3.6e-33 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 545
Acc36I ACCTGC 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 557
AciI CCGC 2 cut(s) 143, 654
AclWI GGATC 1 cut(s) 14
AcoI YGGCCR 2 cut(s) 95, 170
AcsI RAATTY 1 cut(s) 104
AfaI GTAC 2 cut(s) 349, 416
AgsI TTSAA 2 cut(s) 437, 485
AjnI CCWGG 1 cut(s) 536
AluBI AGCT 6 cut(s) 50, 299, 367, 395, 430, 528
AluI AGCT 6 cut(s) 50, 299, 367, 395, 430, 528
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 414
Alw44I GTGCAC 1 cut(s) 158
AlwI GGATC 1 cut(s) 14
AoxI GGCC 6 cut(s) 95, 163, 170, 243, 284, 515
ApaLI GTGCAC 1 cut(s) 158
ApeKI GCWGC 5 cut(s) 47, 56, 88, 145, 528
ApoI RAATTY 1 cut(s) 104
AspS9I GGNCC 4 cut(s) 244, 285, 370, 534
AsuII TTCGAA 1 cut(s) 274
AvaII GGWCC 2 cut(s) 370, 534
BaeGI GKGCMC 1 cut(s) 162
BalI TGGCCA 1 cut(s) 172
BanI GGYRCC 1 cut(s) 557
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 5 cut(s) 59, 68, 100, 157, 515
BccI CCATC 2 cut(s) 181, 235
BceAI ACGGC 2 cut(s) 178, 191
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 1 cut(s) 538
BciVI GTATCC 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 545
BfuI GTATCC 1 cut(s) 140
BisI GCNGC 5 cut(s) 48, 57, 89, 146, 529
BlpI GCTNAGC 1 cut(s) 524
BlsI GCNGC 5 cut(s) 49, 58, 90, 147, 530
Bme1390I CCNGG 1 cut(s) 538
Bme18I GGWCC 2 cut(s) 370, 534
BmgT120I GGNCC 4 cut(s) 244, 285, 370, 534
BmiI GGNNCC 2 cut(s) 287, 559
BmrFI CCNGG 1 cut(s) 538
BmsI GCATC 2 cut(s) 43, 193
Bpu1102I GCTNAGC 1 cut(s) 524
Bpu14I TTCGAA 1 cut(s) 274
Bsa29I ATCGAT 1 cut(s) 630
BsaI GGTCTC 1 cut(s) 414
BsaWI WCCGGW 1 cut(s) 399
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 98
BseBI CCWGG 1 cut(s) 538
BseCI ATCGAT 1 cut(s) 630
BseGI GGATG 4 cut(s) 9, 173, 208, 408
BseMI GCAATG 1 cut(s) 98
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 602
BseSI GKGCMC 1 cut(s) 162
BseXI GCAGC 5 cut(s) 59, 68, 100, 157, 515
BsgI GTGCAG 1 cut(s) 107
BshFI GGCC 6 cut(s) 97, 165, 172, 245, 286, 517
BshNI GGYRCC 1 cut(s) 557
BshVI ATCGAT 1 cut(s) 630
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 2 cut(s) 98, 400
BslFI GGGAC 2 cut(s) 125, 517
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 2 cut(s) 125, 517
BsnI GGCC 6 cut(s) 97, 165, 172, 245, 286, 517
Bso31I GGTCTC 1 cut(s) 414
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 1 cut(s) 162
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 1 cut(s) 6
Bsp1720I GCTNAGC 1 cut(s) 524
BspACI CCGC 2 cut(s) 143, 654
BspANI GGCC 6 cut(s) 97, 165, 172, 245, 286, 517
BspCNI CTCAG 1 cut(s) 455
BspDI ATCGAT 1 cut(s) 630
BspLI GGNNCC 2 cut(s) 287, 559
BspMI ACCTGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 14
BspQI GCTCTTC 1 cut(s) 271
BspT104I TTCGAA 1 cut(s) 274
BspT107I GGYRCC 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 98
BsrFI RCCGGY 1 cut(s) 97
BsrGI TGTACA 1 cut(s) 414
BsrI ACTGG 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 97
BssMI GATC 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 538
Bst4CI ACNGT 4 cut(s) 157, 254, 347, 455
Bst6I CTCTTC 1 cut(s) 271
BstAPI GCANNNNNTGC 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 414
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 2 cut(s) 99, 515
BstDEI CTNAG 2 cut(s) 442, 524
BstF5I GGATG 4 cut(s) 9, 173, 208, 408
BstKTI GATC 1 cut(s) 9
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 4 cut(s) 44, 53, 56, 94
BstNI CCWGG 1 cut(s) 538
BstSCI CCNGG 1 cut(s) 536
BstSLI GKGCMC 1 cut(s) 162
BstV1I GCAGC 5 cut(s) 59, 68, 100, 157, 515
Bsu15I ATCGAT 1 cut(s) 630
BsuI GTATCC 1 cut(s) 140
BsuRI GGCC 6 cut(s) 97, 165, 172, 245, 286, 517
BsuTUI ATCGAT 1 cut(s) 630
BtsCI GGATG 4 cut(s) 9, 173, 208, 408
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 474
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 2 cut(s) 99, 515
Cfr10I RCCGGY 1 cut(s) 97
Cfr13I GGNCC 4 cut(s) 244, 285, 370, 534
ClaI ATCGAT 1 cut(s) 630
CseI GACGC 1 cut(s) 385
Csp6I GTAC 2 cut(s) 348, 415
CviAII CATG 2 cut(s) 186, 282
CviQI GTAC 2 cut(s) 348, 415
DdeI CTNAG 2 cut(s) 442, 524
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EaeI YGGCCR 2 cut(s) 95, 170
Eam1104I CTCTTC 1 cut(s) 271
EarI CTCTTC 1 cut(s) 271
Eco31I GGTCTC 1 cut(s) 414
Eco47I GGWCC 2 cut(s) 370, 534
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 536
FaeI CATG 2 cut(s) 189, 285
FaiI YATR 9 cut(s) 69, 114, 187, 283, 314, 468, 545, 567, 667
FaqI GGGAC 2 cut(s) 125, 517
FatI CATG 2 cut(s) 185, 281
FauI CCCGC 1 cut(s) 150
Fnu4HI GCNGC 5 cut(s) 48, 57, 89, 146, 529
FokI GGATG 3 cut(s) 160, 215, 415
Fsp4HI GCNGC 5 cut(s) 48, 57, 89, 146, 529
FspBI CTAG 1 cut(s) 507
GluI GCNGC 5 cut(s) 48, 57, 89, 146, 529
HaeIII GGCC 6 cut(s) 97, 165, 172, 245, 286, 517
HapII CCGG 2 cut(s) 98, 400
HgaI GACGC 1 cut(s) 385
Hin1II CATG 2 cut(s) 189, 285
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 2 cut(s) 118, 447
HpaII CCGG 2 cut(s) 98, 400
Hpy166II GTNNAC 2 cut(s) 160, 458
Hpy188I TCNGA 2 cut(s) 358, 390
Hpy188III TCNNGA 1 cut(s) 444
Hpy8I GTNNAC 2 cut(s) 160, 458
Hpy99I CGWCG 2 cut(s) 645, 678
HpyCH4III ACNGT 4 cut(s) 157, 254, 347, 455
HpyCH4IV ACGT 3 cut(s) 124, 460, 624
HpyCH4V TGCA 3 cut(s) 56, 88, 160
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 53, 56, 94
HpyF3I CTNAG 2 cut(s) 442, 524
HpySE526I ACGT 3 cut(s) 124, 460, 624
Hsp92II CATG 2 cut(s) 189, 285
KroI GCCGGC 1 cut(s) 97
KroNI GCCGGC 1 cut(s) 99
Kzo9I GATC 1 cut(s) 6
LguI GCTCTTC 1 cut(s) 271
Lsp1109I GCAGC 5 cut(s) 59, 68, 100, 157, 515
LweI GCATC 2 cut(s) 43, 193
MaeI CTAG 1 cut(s) 507
MaeII ACGT 3 cut(s) 124, 460, 624
MaeIII GTNAC 3 cut(s) 461, 658, 677
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 3 cut(s) 20, 263, 288
MfeI CAATTG 2 cut(s) 257, 290
MhlI GDGCHC 1 cut(s) 162
MlsI TGGCCA 1 cut(s) 172
MluCI AATT 6 cut(s) 104, 224, 234, 257, 290, 649
MluNI TGGCCA 1 cut(s) 172
MnlI CCTC 5 cut(s) 494, 503, 528, 580, 583
Mox20I TGGCCA 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 97
MscI TGGCCA 1 cut(s) 172
MseI TTAA 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 172
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 2 cut(s) 98, 400
MspR9I CCNGG 1 cut(s) 538
MunI CAATTG 2 cut(s) 257, 290
MvaI CCWGG 1 cut(s) 538
MwoI GCNNNNNNNGC 4 cut(s) 44, 53, 56, 94
NaeI GCCGGC 1 cut(s) 99
NdeII GATC 1 cut(s) 6
NgoMIV GCCGGC 1 cut(s) 97
NlaIII CATG 2 cut(s) 189, 285
NlaIV GGNNCC 2 cut(s) 287, 559
NspV TTCGAA 1 cut(s) 274
PaqCI CACCTGC 1 cut(s) 545
PciSI GCTCTTC 1 cut(s) 271
PdiI GCCGGC 1 cut(s) 99
PfeI GAWTC 2 cut(s) 118, 447
PfoI TCCNGGA 1 cut(s) 536
PkrI GCNGC 5 cut(s) 49, 58, 90, 147, 530
Psp6I CCWGG 1 cut(s) 536
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 2 cut(s) 287, 559
PspPI GGNCC 4 cut(s) 244, 285, 370, 534
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 2 cut(s) 349, 416
RsaNI GTAC 2 cut(s) 348, 415
SapI GCTCTTC 1 cut(s) 271
SaqAI TTAA 1 cut(s) 228
SatI GCNGC 5 cut(s) 48, 57, 89, 146, 529
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 4 cut(s) 244, 285, 370, 534
ScrFI CCNGG 1 cut(s) 538
SduI GDGCHC 1 cut(s) 162
SfaNI GCATC 2 cut(s) 43, 193
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 2 cut(s) 370, 534
Sse9I AATT 6 cut(s) 104, 224, 234, 257, 290, 649
SsiI CCGC 2 cut(s) 143, 654
SspI AATATT 1 cut(s) 383
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 1 cut(s) 536
TaaI ACNGT 4 cut(s) 157, 254, 347, 455
TaiI ACGT 3 cut(s) 127, 463, 627
TaqI TCGA 3 cut(s) 274, 630, 640
TasI AATT 6 cut(s) 104, 224, 234, 257, 290, 649
TatI WGTACW 1 cut(s) 414
TfiI GAWTC 2 cut(s) 118, 447
Tru1I TTAA 1 cut(s) 228
Tru9I TTAA 1 cut(s) 228
TscAI CASTG 1 cut(s) 481
TseI GCWGC 5 cut(s) 47, 56, 88, 145, 528
TspDTI ATGAA 3 cut(s) 246, 345, 582
TspGWI ACGGA 1 cut(s) 658
TspRI CASTG 1 cut(s) 481
VneI GTGCAC 1 cut(s) 158
VpaK11BI GGWCC 2 cut(s) 370, 534
XapI RAATTY 1 cut(s) 104
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.