RLG00000017904

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000345
Physical Location & Seq
Reverse (-)
22815082 .. 22815885
804 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017904

Sequence Viewer

Length: 711 bp
ATGAAGAATTCATACTTCATTATCCTTCTATCAGTATATGCACTTGTACAAGTTCTCAACGTTTCAAATGCTGAACCCTACGAGTACATGCAATTTGTACTCCAGTACCCACCTGGAGTTTGCTATGGCAAATCAAAAGGTGATTGCATCTCACCTCTCCCAACCAAGTTTCATGTACATGGAATATGGCCTTCTAACTTCTCCAATATACTTGTCCATTGTGGGCACGCACTGAGGAATAACCCCTTTCATAAGGCGCAGATGACACCTCTGCAGACGGATTTGGGAAACTCATGGCCGTCCGTGTTAACACAAAACAACAATATGCAGTTCTGGGAGCATGAGTATGGAGAGCACGGTGCATGTGCAGTAGAGTCAGGTGTACCACCTTTCACTCAAAGGTCCTACTTTGAGAAAGGACACCAGCTATGGAACCAATATGACATCCTTGCAGTGCTTCATCAAAGTGGTATTAAACCGGGTACTGCAACATCATACACTATGGCTCAGCTTGTAACCGCAATCCAAAAGAAAATCGGGAGTAACAATACTCCTTTAATCATGTGCACGAGGACAGCCCTTGGTTACACACTAAAGGAAGTGATAATCTGTCTGGATCACCAGGCAACAAATGTGATAAGCTGCGCGCTCAATAATATTAGAAAAACAGATTGCCGAGATCCTTCGGGCAAGGTATACTATGTAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

237

Amino Acids

26.47

Weight (kDa)

8.37

Isoelectric Point (pI)

33.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 215 9.8e-40 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 696
AccII CGCG 1 cut(s) 647
AciI CCGC 1 cut(s) 519
AclI AACGTT 1 cut(s) 60
AclWI GGATC 2 cut(s) 624, 674
AcoI YGGCCR 1 cut(s) 296
AcsI RAATTY 1 cut(s) 7
AfaI GTAC 7 cut(s) 48, 86, 99, 107, 177, 384, 484
AgsI TTSAA 1 cut(s) 66
AjnI CCWGG 2 cut(s) 112, 621
AluBI AGCT 4 cut(s) 427, 511, 642, 707
AluI AGCT 4 cut(s) 427, 511, 642, 707
Alw21I GWGCWC 2 cut(s) 357, 569
Alw44I GTGCAC 1 cut(s) 565
AlwI GGATC 2 cut(s) 624, 674
AoxI GGCC 2 cut(s) 188, 296
ApaLI GTGCAC 1 cut(s) 565
ApeKI GCWGC 1 cut(s) 642
ApoI RAATTY 1 cut(s) 7
AspLEI GCGC 3 cut(s) 259, 647, 649
AspS9I GGNCC 1 cut(s) 402
AsuC2I CCSGG 1 cut(s) 480
AsuHPI GGTGA 3 cut(s) 144, 152, 611
AvaII GGWCC 1 cut(s) 402
BaeGI GKGCMC 2 cut(s) 228, 569
BaeI ACNNNNGTAYC 2 cut(s) 89, 122
BarI GAAGNNNNNNTAC 1 cut(s) 28
BauI CACGAG 1 cut(s) 568
Bbv12I GWGCWC 2 cut(s) 357, 569
BbvI GCAGC 1 cut(s) 629
BceAI ACGGC 1 cut(s) 283
BciT130I CCWGG 2 cut(s) 114, 623
BcnI CCSGG 1 cut(s) 480
BfmI CTRYAG 1 cut(s) 272
BisI GCNGC 1 cut(s) 643
BlpI GCTNAGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 644
Bme1390I CCNGG 3 cut(s) 114, 480, 623
Bme18I GGWCC 1 cut(s) 402
BmgT120I GGNCC 1 cut(s) 402
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 3 cut(s) 114, 480, 623
BmsI GCATC 1 cut(s) 156
BpmI CTGGAG 2 cut(s) 86, 135
Bpu1102I GCTNAGC 1 cut(s) 507
BpuMI CCSGG 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 580
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bse1I ACTGG 1 cut(s) 103
BseBI CCWGG 2 cut(s) 114, 623
BseDI CCNNGG 1 cut(s) 580
BseGI GGATG 1 cut(s) 444
BseMII CTCAG 2 cut(s) 224, 521
BseNI ACTGG 1 cut(s) 103
BsePI GCGCGC 1 cut(s) 645
BseSI GKGCMC 2 cut(s) 228, 569
BseXI GCAGC 1 cut(s) 629
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 1 cut(s) 647
BshFI GGCC 2 cut(s) 190, 298
BsiHKAI GWGCWC 2 cut(s) 357, 569
BsiSI CCGG 1 cut(s) 479
BsnI GGCC 2 cut(s) 190, 298
Bsp1286I GDGCHC 3 cut(s) 228, 357, 569
Bsp1407I TGTACA 2 cut(s) 46, 175
Bsp143I GATC 2 cut(s) 616, 679
Bsp1720I GCTNAGC 1 cut(s) 507
BspACI CCGC 1 cut(s) 519
BspANI GGCC 2 cut(s) 190, 298
BspCNI CTCAG 2 cut(s) 225, 520
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 1 cut(s) 434
BspMAI CTGCAG 1 cut(s) 276
BspPI GGATC 2 cut(s) 624, 674
BsrGI TGTACA 2 cut(s) 46, 175
BsrI ACTGG 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 580
BssHII GCGCGC 1 cut(s) 645
BssMI GATC 2 cut(s) 616, 679
BssNAI GTATAC 1 cut(s) 697
BssSI CACGAG 1 cut(s) 568
BssT1I CCWWGG 1 cut(s) 580
Bst1107I GTATAC 1 cut(s) 697
Bst2BI CACGAG 1 cut(s) 568
Bst2UI CCWGG 2 cut(s) 114, 623
Bst4CI ACNGT 1 cut(s) 359
BstAUI TGTACA 2 cut(s) 46, 175
BstC8I GCNNGC 2 cut(s) 228, 647
BstDEI CTNAG 3 cut(s) 233, 507, 708
BstF5I GGATG 1 cut(s) 444
BstFNI CGCG 1 cut(s) 647
BstHHI GCGC 3 cut(s) 259, 647, 649
BstKTI GATC 2 cut(s) 619, 682
BstMBI GATC 2 cut(s) 616, 679
BstNI CCWGG 2 cut(s) 114, 623
BstNSI RCATGY 2 cut(s) 91, 366
BstSCI CCNGG 3 cut(s) 112, 478, 621
BstSFI CTRYAG 1 cut(s) 272
BstSLI GKGCMC 2 cut(s) 228, 569
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 1 cut(s) 629
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
BstZ17I GTATAC 1 cut(s) 697
BsuRI GGCC 2 cut(s) 190, 298
BtsCI GGATG 1 cut(s) 444
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 2 cut(s) 230, 459
Cac8I GCNNGC 2 cut(s) 228, 647
CfoI GCGC 3 cut(s) 259, 647, 649
Cfr13I GGNCC 1 cut(s) 402
Csp6I GTAC 7 cut(s) 47, 85, 98, 106, 176, 383, 483
CviAII CATG 7 cut(s) 88, 173, 179, 294, 341, 363, 562
CviJI RGCY 8 cut(s) 190, 298, 427, 506, 511, 578, 642, 707
CviKI_1 RGCY 8 cut(s) 190, 298, 427, 506, 511, 578, 642, 707
CviQI GTAC 7 cut(s) 47, 85, 98, 106, 176, 383, 483
DdeI CTNAG 3 cut(s) 233, 507, 708
DpnI GATC 2 cut(s) 618, 681
DpnII GATC 2 cut(s) 616, 679
EaeI YGGCCR 1 cut(s) 296
Eco130I CCWWGG 1 cut(s) 580
Eco47I GGWCC 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 112, 621
EcoT14I CCWWGG 1 cut(s) 580
ErhI CCWWGG 1 cut(s) 580
FaeI CATG 7 cut(s) 91, 176, 182, 297, 344, 366, 565
FatI CATG 7 cut(s) 87, 172, 178, 293, 340, 362, 561
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 1 cut(s) 643
FokI GGATG 1 cut(s) 431
Fsp4HI GCNGC 1 cut(s) 643
GlaI GCGC 3 cut(s) 258, 646, 648
GluI GCNGC 1 cut(s) 643
GsuI CTGGAG 2 cut(s) 86, 135
HaeIII GGCC 2 cut(s) 190, 298
HapII CCGG 1 cut(s) 479
HhaI GCGC 3 cut(s) 259, 647, 649
Hin1II CATG 7 cut(s) 91, 176, 182, 297, 344, 366, 565
Hin6I GCGC 3 cut(s) 257, 645, 647
HinP1I GCGC 3 cut(s) 257, 645, 647
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 1 cut(s) 374
HpaI GTTAAC 1 cut(s) 309
HpaII CCGG 1 cut(s) 479
HphI GGTGA 3 cut(s) 144, 152, 611
Hpy166II GTNNAC 4 cut(s) 309, 383, 567, 697
Hpy188III TCNNGA 2 cut(s) 538, 614
Hpy8I GTNNAC 4 cut(s) 309, 383, 567, 697
HpyAV CCTTC 3 cut(s) 35, 201, 693
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4IV ACGT 1 cut(s) 60
HpyF3I CTNAG 3 cut(s) 233, 507, 708
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 7 cut(s) 91, 176, 182, 297, 344, 366, 565
HspAI GCGC 3 cut(s) 257, 645, 647
KspAI GTTAAC 1 cut(s) 309
Kzo9I GATC 2 cut(s) 616, 679
LmnI GCTCC 1 cut(s) 337
Lsp1109I GCAGC 1 cut(s) 629
LweI GCATC 1 cut(s) 156
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 3 cut(s) 514, 542, 584
MalI GATC 2 cut(s) 618, 681
MboI GATC 2 cut(s) 616, 679
MboII GAAGA 1 cut(s) 16
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 3 cut(s) 228, 357, 569
MluCI AATT 2 cut(s) 7, 92
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 4 cut(s) 165, 228, 279, 564
MseI TTAA 3 cut(s) 308, 474, 557
MslI CAYNNNNRTG 3 cut(s) 177, 345, 465
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 3 cut(s) 114, 480, 623
MvaI CCWGG 2 cut(s) 114, 623
MvnI CGCG 1 cut(s) 647
NciI CCSGG 1 cut(s) 480
NdeII GATC 2 cut(s) 616, 679
NlaIII CATG 7 cut(s) 91, 176, 182, 297, 344, 366, 565
NlaIV GGNNCC 1 cut(s) 434
NmeAIII GCCGAG 1 cut(s) 701
NspI RCATGY 2 cut(s) 91, 366
PauI GCGCGC 1 cut(s) 645
PkrI GCNGC 1 cut(s) 644
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PpuMI RGGWCCY 1 cut(s) 402
Psp1406I AACGTT 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 402
Psp6I CCWGG 2 cut(s) 112, 621
PspGI CCWGG 2 cut(s) 112, 621
PspN4I GGNNCC 1 cut(s) 434
PspPI GGNCC 1 cut(s) 402
PspPPI RGGWCCY 1 cut(s) 402
PstI CTGCAG 1 cut(s) 276
PsuI RGATCY 1 cut(s) 679
PteI GCGCGC 1 cut(s) 645
RsaI GTAC 7 cut(s) 48, 86, 99, 107, 177, 384, 484
RsaNI GTAC 7 cut(s) 47, 85, 98, 106, 176, 383, 483
RseI CAYNNNNRTG 3 cut(s) 177, 345, 465
SaqAI TTAA 3 cut(s) 308, 474, 557
SatI GCNGC 1 cut(s) 643
Sau3AI GATC 2 cut(s) 616, 679
Sau96I GGNCC 1 cut(s) 402
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 3 cut(s) 114, 480, 623
SduI GDGCHC 3 cut(s) 228, 357, 569
SfaNI GCATC 1 cut(s) 156
SfcI CTRYAG 1 cut(s) 272
SinI GGWCC 1 cut(s) 402
SmiMI CAYNNNNRTG 3 cut(s) 177, 345, 465
Sse9I AATT 2 cut(s) 7, 92
SsiI CCGC 1 cut(s) 519
SspI AATATT 1 cut(s) 658
StyD4I CCNGG 3 cut(s) 112, 478, 621
StyI CCWWGG 1 cut(s) 580
TaaI ACNGT 1 cut(s) 359
TaiI ACGT 1 cut(s) 63
TasI AATT 2 cut(s) 7, 92
TatI WGTACW 4 cut(s) 46, 84, 97, 175
Tru1I TTAA 3 cut(s) 308, 474, 557
Tru9I TTAA 3 cut(s) 308, 474, 557
TscAI CASTG 2 cut(s) 237, 459
TseI GCWGC 1 cut(s) 642
TspDTI ATGAA 5 cut(s) 7, 17, 161, 239, 449
TspGWI ACGGA 2 cut(s) 292, 293
TspRI CASTG 2 cut(s) 237, 459
VneI GTGCAC 1 cut(s) 565
VpaK11BI GGWCC 1 cut(s) 402
XapI RAATTY 1 cut(s) 7
XceI RCATGY 2 cut(s) 91, 366
XcmI CCANNNNNNNNNTGG 1 cut(s) 110
XmiI GTMKAC 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.