RLG00000025224

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000345
Physical Location & Seq
Reverse (-)
43103277 .. 43106227
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025224

Sequence Viewer

Length: 1068 bp
ATGGGGATTGTCCTCCTGATTCTTCTCTGTGCTTTTCGGGTGGCTAGTGCAGCCAACACATATGATTATCTCCAACTAGTCCTGCAGTGGCCAAACACGTACTGCATCTACCAAAACTGCATATCAAACCTGCCACTCGACTTCACCATACATGGGTTGTGGCCTAGCAATTTCTCCGGCCAAAACAACCCATGTGTGGGCACGCCATTTAATAGAGCTCAGATGTCCGCGCACTATCGTCTTCAAACCATATTGCTCCCATCCTCGTGGAGAAGCTACCTTATCGGTGTCTCCAACACAGACTTCTGGGAGGGCGAGTATAACAAACATGGCACATGTTCGGAGCTCAATATTCCACAGACGGAATACTTCACCAAAACCCATTGGCTATGGATGGCCAACAACATTTACAGCATACTTTCATCTTCGGTCAACAGACAAGCATATCTAATCTTGCCGGGTTACACCTATGACTACACCGATCTTCTTGCCGCTGTTCAACTAGGAATCGGTGGCGTCACTCCCTTGCTTACTTGCAAGATCATAAATCAAAACCAATACGTTCTTCACGAAGTGGTTATTTGTTATGATGCCTTGGGGATCAACCGCATCCATTGTGTGCGACAATCGAATTGCCTTTTCGCTCCTGGCGGACTACAAATAAAAATTTCACCACACTCTCACGTGACATCACGTGCTCAACATTTCTCTACCGCTGATCGGAATCTCCGCCGGCGATTGAAGGTCGGTCAGACGGTGCAGCCGCAGAAGACGAAGAAGAAGAAGAGCATCGAGACCAAGGCTACCGACTCTATCGCCGTGACCATCGACCAGACGACGTCGTCCACCACGATCGACAGGCTCAGCTCCGGCAACCAACTCCGCGGGGTCCACCCCATCGATGACCGGAATGCTGACGTGAACCGCCACCAGGACATTTCTAAGCTTCTACTCTCGACTTCGAGTAGGTTTTGTAAAAGAGCGGCTAGGGGGTGGCAGCTCGGCAGGTTTGGTATTTATATAGGGAAGGAAGACGGTGATGGCAAAGTTGTAATTATGAGGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

356

Amino Acids

40.02

Weight (kDa)

9.31

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 21 - 206 3.7e-28 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 841
AatII GACGTC 1 cut(s) 842
Acc36I ACCTGC 2 cut(s) 138, 996
AccBSI CCGCTC 1 cut(s) 983
AccII CGCG 2 cut(s) 230, 885
AclWI GGATC 1 cut(s) 608
AcoI YGGCCR 3 cut(s) 89, 178, 396
AcsI RAATTY 1 cut(s) 666
AcvI CACGTG 2 cut(s) 685, 695
AcyI GRCGYC 2 cut(s) 516, 839
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 1 cut(s) 101
AfiI CCNNNNNNNGG 5 cut(s) 153, 196, 197, 720, 931
AflIII ACRYGT 2 cut(s) 96, 335
AgsI TTSAA 3 cut(s) 245, 500, 742
AhlI ACTAGT 1 cut(s) 76
AjiI CACGTC 1 cut(s) 919
AjnI CCWGG 2 cut(s) 646, 930
AjuI GAANNNNNNNTTGG 4 cut(s) 287, 319, 549, 581
AluBI AGCT 6 cut(s) 218, 276, 346, 867, 946, 1000
AluI AGCT 6 cut(s) 218, 276, 346, 867, 946, 1000
Alw21I GWGCWC 3 cut(s) 220, 348, 700
Alw26I GTCTC 2 cut(s) 295, 788
AlwI GGATC 1 cut(s) 608
AoxI GGCC 4 cut(s) 89, 161, 178, 396
ApeKI GCWGC 3 cut(s) 50, 760, 997
ApoI RAATTY 1 cut(s) 666
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 889
AsuC2I CCSGG 1 cut(s) 459
AsuHPI GGTGA 4 cut(s) 136, 364, 663, 1049
AvaII GGWCC 1 cut(s) 889
BaeGI GKGCMC 1 cut(s) 203
BalI TGGCCA 2 cut(s) 91, 398
BanII GRGCYC 2 cut(s) 220, 348
BauI CACGAG 1 cut(s) 265
BbrPI CACGTG 2 cut(s) 685, 695
BbsI GAAGAC 3 cut(s) 233, 776, 1038
Bbv12I GWGCWC 3 cut(s) 220, 348, 700
BbvI GCAGC 3 cut(s) 62, 772, 1009
BccI CCATC 5 cut(s) 268, 388, 833, 905, 1034
BceAI ACGGC 1 cut(s) 803
BcgI CGANNNNNNTGC 2 cut(s) 470, 504
BciT130I CCWGG 2 cut(s) 648, 932
BcnI CCSGG 1 cut(s) 459
BcoDI GTCTC 2 cut(s) 295, 788
BcuI ACTAGT 1 cut(s) 76
BfaI CTAG 5 cut(s) 45, 77, 165, 503, 987
BfmI CTRYAG 1 cut(s) 83
BfuAI ACCTGC 2 cut(s) 138, 996
BisI GCNGC 6 cut(s) 51, 492, 761, 764, 984, 998
BlpI GCTNAGC 1 cut(s) 863
BlsI GCNGC 6 cut(s) 52, 493, 762, 765, 985, 999
Bme1390I CCNGG 3 cut(s) 459, 648, 932
Bme18I GGWCC 1 cut(s) 889
BmgBI CACGTC 1 cut(s) 919
BmgT120I GGNCC 1 cut(s) 889
BmiI GGNNCC 1 cut(s) 890
BmrFI CCNGG 3 cut(s) 459, 648, 932
BmsI GCATC 4 cut(s) 114, 580, 618, 798
BpiI GAAGAC 3 cut(s) 233, 776, 1038
Bpu1102I GCTNAGC 1 cut(s) 863
BpuMI CCSGG 1 cut(s) 459
Bsa29I ATCGAT 1 cut(s) 900
BsaAI YACGTR 3 cut(s) 99, 685, 695
BsaBI GATNNNNATC 1 cut(s) 723
BsaHI GRCGYC 2 cut(s) 516, 839
BsaI GGTCTC 1 cut(s) 788
BsaJI CCNNGG 3 cut(s) 594, 798, 883
BsaWI WCCGGW 1 cut(s) 906
BsaXI ACNNNNNCTCC 2 cut(s) 302, 332
Bsc4I CCNNNNNNNGG 5 cut(s) 153, 196, 197, 720, 931
Bse118I RCCGGY 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 723
BseBI CCWGG 2 cut(s) 648, 932
BseCI ATCGAT 1 cut(s) 900
BseDI CCNNGG 3 cut(s) 594, 798, 883
BseGI GGATG 3 cut(s) 260, 399, 609
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 5 cut(s) 153, 196, 197, 720, 931
BseMII CTCAG 2 cut(s) 233, 877
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 3 cut(s) 62, 772, 1009
BsgI GTGCAG 2 cut(s) 69, 779
Bsh1236I CGCG 2 cut(s) 230, 885
Bsh1285I CGRYCG 1 cut(s) 855
BshFI GGCC 4 cut(s) 91, 163, 180, 398
BshVI ATCGAT 1 cut(s) 900
BsiEI CGRYCG 1 cut(s) 855
BsiHKAI GWGCWC 3 cut(s) 220, 348, 700
BsiSI CCGG 5 cut(s) 177, 458, 733, 870, 907
BslI CCNNNNNNNGG 5 cut(s) 153, 196, 197, 720, 931
BsmAI GTCTC 2 cut(s) 295, 788
BsmI GAATGC 1 cut(s) 916
BsnI GGCC 4 cut(s) 91, 163, 180, 398
Bso31I GGTCTC 1 cut(s) 788
Bsp1286I GDGCHC 4 cut(s) 203, 220, 348, 700
Bsp143I GATC 5 cut(s) 481, 540, 600, 718, 852
Bsp1720I GCTNAGC 1 cut(s) 863
BspANI GGCC 4 cut(s) 91, 163, 180, 398
BspCNI CTCAG 2 cut(s) 232, 876
BspDI ATCGAT 1 cut(s) 900
BspFNI CGCG 2 cut(s) 230, 885
BspLI GGNNCC 1 cut(s) 890
BspMAI CTGCAG 1 cut(s) 87
BspMI ACCTGC 2 cut(s) 138, 996
BspPI GGATC 1 cut(s) 608
BspQI GCTCTTC 1 cut(s) 779
BspTNI GGTCTC 1 cut(s) 788
BsrBI CCGCTC 1 cut(s) 983
BsrFI RCCGGY 1 cut(s) 732
BssAI RCCGGY 1 cut(s) 732
BssECI CCNNGG 3 cut(s) 594, 798, 883
BssMI GATC 5 cut(s) 481, 540, 600, 718, 852
BssNI GRCGYC 2 cut(s) 516, 839
BssSI CACGAG 1 cut(s) 265
BssT1I CCWWGG 2 cut(s) 594, 798
Bst2BI CACGAG 1 cut(s) 265
Bst2UI CCWGG 2 cut(s) 648, 932
Bst4CI ACNGT 2 cut(s) 757, 1037
Bst6I CTCTTC 1 cut(s) 779
BstACI GRCGYC 2 cut(s) 516, 839
BstBAI YACGTR 3 cut(s) 99, 685, 695
BstC8I GCNNGC 2 cut(s) 203, 734
BstDEI CTNAG 3 cut(s) 219, 863, 942
BstDSI CCRYGG 1 cut(s) 883
BstF5I GGATG 3 cut(s) 260, 399, 609
BstFNI CGCG 2 cut(s) 230, 885
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 5 cut(s) 484, 543, 603, 721, 855
BstMAI GTCTC 2 cut(s) 295, 788
BstMBI GATC 5 cut(s) 481, 540, 600, 718, 852
BstMCI CGRYCG 1 cut(s) 855
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 2 cut(s) 648, 932
BstNSI RCATGY 1 cut(s) 339
BstSCI CCNGG 3 cut(s) 457, 646, 930
BstSFI CTRYAG 1 cut(s) 83
BstSLI GKGCMC 1 cut(s) 203
BstUI CGCG 2 cut(s) 230, 885
BstV1I GCAGC 3 cut(s) 62, 772, 1009
BstV2I GAAGAC 3 cut(s) 233, 776, 1038
BstXI CCANNNNNNTGG 1 cut(s) 267
Bsu15I ATCGAT 1 cut(s) 900
BsuRI GGCC 4 cut(s) 91, 163, 180, 398
BsuTUI ATCGAT 1 cut(s) 900
BtgI CCRYGG 1 cut(s) 883
BtrI CACGTC 1 cut(s) 919
BtsCI GGATG 3 cut(s) 260, 399, 609
BtsI GCAGTG 1 cut(s) 92
BtsIMutI CAGTG 1 cut(s) 92
BveI ACCTGC 2 cut(s) 138, 996
Cac8I GCNNGC 2 cut(s) 203, 734
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 732
Cfr13I GGNCC 1 cut(s) 889
Cfr42I CCGCGG 1 cut(s) 886
ClaI ATCGAT 1 cut(s) 900
CseI GACGC 1 cut(s) 505
Csp6I GTAC 1 cut(s) 100
CviAII CATG 4 cut(s) 152, 192, 329, 336
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 3 cut(s) 219, 863, 942
DpnI GATC 5 cut(s) 483, 542, 602, 720, 854
DpnII GATC 5 cut(s) 481, 540, 600, 718, 852
DraIII CACNNNGTG 1 cut(s) 574
DrdI GACNNNNNNGTC 1 cut(s) 841
DseDI GACNNNNNNGTC 1 cut(s) 841
EaeI YGGCCR 3 cut(s) 89, 178, 396
Eam1104I CTCTTC 1 cut(s) 779
EarI CTCTTC 1 cut(s) 779
EciI GGCGGA 2 cut(s) 666, 719
Ecl136II GAGCTC 2 cut(s) 218, 346
Eco130I CCWWGG 2 cut(s) 594, 798
Eco24I GRGCYC 2 cut(s) 220, 348
Eco31I GGTCTC 1 cut(s) 788
Eco47I GGWCC 1 cut(s) 889
Eco53kI GAGCTC 2 cut(s) 218, 346
Eco72I CACGTG 2 cut(s) 685, 695
EcoICRI GAGCTC 2 cut(s) 218, 346
EcoRII CCWGG 2 cut(s) 646, 930
EcoT14I CCWWGG 2 cut(s) 594, 798
EcoT38I GRGCYC 2 cut(s) 220, 348
ErhI CCWWGG 2 cut(s) 594, 798
FaeI CATG 4 cut(s) 155, 195, 332, 339
FatI CATG 4 cut(s) 151, 191, 328, 335
FauI CCCGC 1 cut(s) 878
FauNDI CATATG 1 cut(s) 61
Fnu4HI GCNGC 6 cut(s) 51, 492, 761, 764, 984, 998
FokI GGATG 3 cut(s) 247, 406, 596
FriOI GRGCYC 2 cut(s) 220, 348
Fsp4HI GCNGC 6 cut(s) 51, 492, 761, 764, 984, 998
FspBI CTAG 5 cut(s) 45, 77, 165, 503, 987
GlaI GCGC 1 cut(s) 231
GluI GCNGC 6 cut(s) 51, 492, 761, 764, 984, 998
HaeIII GGCC 4 cut(s) 91, 163, 180, 398
HapII CCGG 5 cut(s) 177, 458, 733, 870, 907
HgaI GACGC 1 cut(s) 505
HhaI GCGC 1 cut(s) 232
Hin1I GRCGYC 2 cut(s) 516, 839
Hin1II CATG 4 cut(s) 155, 195, 332, 339
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HindIII AAGCTT 1 cut(s) 944
HinfI GANTC 4 cut(s) 19, 507, 724, 809
HpaII CCGG 5 cut(s) 177, 458, 733, 870, 907
HphI GGTGA 4 cut(s) 136, 364, 663, 1049
Hpy166II GTNNAC 4 cut(s) 433, 846, 892, 922
Hpy188I TCNGA 4 cut(s) 222, 343, 723, 753
Hpy188III TCNNGA 4 cut(s) 16, 569, 793, 955
Hpy8I GTNNAC 4 cut(s) 433, 846, 892, 922
Hpy99I CGWCG 2 cut(s) 841, 844
HpyAV CCTTC 2 cut(s) 736, 1021
HpyCH4III ACNGT 2 cut(s) 757, 1037
HpyCH4IV ACGT 6 cut(s) 98, 561, 684, 694, 839, 918
HpyCH4V TGCA 6 cut(s) 50, 85, 105, 120, 537, 760
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 3 cut(s) 219, 863, 942
HpySE526I ACGT 6 cut(s) 98, 561, 684, 694, 839, 918
Hsp92I GRCGYC 2 cut(s) 516, 839
Hsp92II CATG 4 cut(s) 155, 195, 332, 339
HspAI GCGC 1 cut(s) 230
KroI GCCGGC 1 cut(s) 732
KroNI GCCGGC 1 cut(s) 734
KspI CCGCGG 1 cut(s) 886
Kzo9I GATC 5 cut(s) 481, 540, 600, 718, 852
LguI GCTCTTC 1 cut(s) 779
LmnI GCTCC 4 cut(s) 261, 343, 649, 872
Lsp1109I GCAGC 3 cut(s) 62, 772, 1009
LweI GCATC 4 cut(s) 114, 580, 618, 798
MaeI CTAG 5 cut(s) 45, 77, 165, 503, 987
MaeII ACGT 6 cut(s) 98, 561, 684, 694, 839, 918
MaeIII GTNAC 4 cut(s) 461, 517, 685, 820
MalI GATC 5 cut(s) 483, 542, 602, 720, 854
MbiI CCGCTC 1 cut(s) 983
MboI GATC 5 cut(s) 481, 540, 600, 718, 852
MhlI GDGCHC 4 cut(s) 203, 220, 348, 700
MlsI TGGCCA 2 cut(s) 91, 398
MluCI AATT 4 cut(s) 169, 631, 666, 1053
MluNI TGGCCA 2 cut(s) 91, 398
MlyI GAGTC 1 cut(s) 803
MmeI TCCRAC 2 cut(s) 97, 318
MnlI CCTC 4 cut(s) 23, 274, 304, 1053
Mox20I TGGCCA 2 cut(s) 91, 398
MreI CGCCGGCG 1 cut(s) 732
MroNI GCCGGC 1 cut(s) 732
MscI TGGCCA 2 cut(s) 91, 398
MseI TTAA 1 cut(s) 210
MslI CAYNNNNRTG 1 cut(s) 265
Msp20I TGGCCA 2 cut(s) 91, 398
MspA1I CMGCKG 3 cut(s) 494, 716, 885
MspI CCGG 5 cut(s) 177, 458, 733, 870, 907
MspR9I CCNGG 3 cut(s) 459, 648, 932
Mva1269I GAATGC 1 cut(s) 916
MvaI CCWGG 2 cut(s) 648, 932
MvnI CGCG 2 cut(s) 230, 885
MwoI GCNNNNNNNGC 1 cut(s) 50
NaeI GCCGGC 1 cut(s) 734
NciI CCSGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 61
NdeII GATC 5 cut(s) 481, 540, 600, 718, 852
NgoMIV GCCGGC 1 cut(s) 732
NlaIII CATG 4 cut(s) 155, 195, 332, 339
NlaIV GGNNCC 1 cut(s) 890
NmeAIII GCCGAG 1 cut(s) 981
NmuCI GTSAC 3 cut(s) 517, 685, 820
NspI RCATGY 1 cut(s) 339
PciI ACATGT 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 779
PcsI WCGNNNNNNNCGW 2 cut(s) 567, 848
PctI GAATGC 1 cut(s) 916
PdiI GCCGGC 1 cut(s) 734
PfeI GAWTC 3 cut(s) 19, 507, 724
PflFI GACNNNGTC 2 cut(s) 838, 841
PkrI GCNGC 6 cut(s) 52, 493, 762, 765, 985, 999
Ple19I CGATCG 1 cut(s) 855
PleI GAGTC 1 cut(s) 803
PmaCI CACGTG 2 cut(s) 685, 695
PmlI CACGTG 2 cut(s) 685, 695
PpsI GAGTC 1 cut(s) 803
Ppu21I YACGTR 3 cut(s) 99, 685, 695
PscI ACATGT 1 cut(s) 335
Psp124BI GAGCTC 2 cut(s) 220, 348
Psp6I CCWGG 2 cut(s) 646, 930
PspCI CACGTG 2 cut(s) 685, 695
PspGI CCWGG 2 cut(s) 646, 930
PspN4I GGNNCC 1 cut(s) 890
PspPI GGNCC 1 cut(s) 889
PstI CTGCAG 1 cut(s) 87
PsyI GACNNNGTC 2 cut(s) 838, 841
PvuI CGATCG 1 cut(s) 855
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
RseI CAYNNNNRTG 1 cut(s) 265
SacI GAGCTC 2 cut(s) 220, 348
SacII CCGCGG 1 cut(s) 886
SapI GCTCTTC 1 cut(s) 779
SaqAI TTAA 1 cut(s) 210
SatI GCNGC 6 cut(s) 51, 492, 761, 764, 984, 998
Sau3AI GATC 5 cut(s) 481, 540, 600, 718, 852
Sau96I GGNCC 1 cut(s) 889
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 3 cut(s) 459, 648, 932
SduI GDGCHC 4 cut(s) 203, 220, 348, 700
SfaNI GCATC 4 cut(s) 114, 580, 618, 798
SfcI CTRYAG 1 cut(s) 83
Sfr303I CCGCGG 1 cut(s) 886
SgrAI CRCCGGYG 1 cut(s) 732
SgrBI CCGCGG 1 cut(s) 886
SinI GGWCC 1 cut(s) 889
SmiMI CAYNNNNRTG 1 cut(s) 265
SpeI ACTAGT 1 cut(s) 76
Sse9I AATT 4 cut(s) 169, 631, 666, 1053
SspI AATATT 1 cut(s) 352
SspMI CTAG 5 cut(s) 45, 77, 165, 503, 987
SstI GAGCTC 2 cut(s) 220, 348
StyD4I CCNGG 3 cut(s) 457, 646, 930
StyI CCWWGG 2 cut(s) 594, 798
TaaI ACNGT 2 cut(s) 757, 1037
TaiI ACGT 6 cut(s) 101, 564, 687, 697, 842, 921
TaqI TCGA 8 cut(s) 138, 629, 792, 828, 855, 900, 956, 962
TaqII GACCGA 2 cut(s) 418, 737
TasI AATT 4 cut(s) 169, 631, 666, 1053
TauI GCSGC 3 cut(s) 494, 766, 986
TfiI GAWTC 3 cut(s) 19, 507, 724
Tru1I TTAA 1 cut(s) 210
Tru9I TTAA 1 cut(s) 210
TscAI CASTG 1 cut(s) 92
TseFI GTSAC 3 cut(s) 517, 685, 820
TseI GCWGC 3 cut(s) 50, 760, 997
Tsp45I GTSAC 3 cut(s) 517, 685, 820
TspDTI ATGAA 1 cut(s) 411
TspGWI ACGGA 1 cut(s) 377
TspRI CASTG 1 cut(s) 92
Tth111I GACNNNGTC 2 cut(s) 838, 841
VpaK11BI GGWCC 1 cut(s) 889
XapI RAATTY 1 cut(s) 666
XceI RCATGY 1 cut(s) 339
XspI CTAG 5 cut(s) 45, 77, 165, 503, 987
ZraI GACGTC 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.