Rroxscaffold_6G00421070

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000345
Physical Location & Seq
Forward (+)
42306964 .. 42325036
18073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00421070.1

Sequence Viewer

Length: 1815 bp
ATGAAAACACCAGCAGTCTGTCTTATTTTCTTGCTCTCTTTTATTCTGTTCAAGTGTTCCCATGCAGAGCCATACGAATACCTACAGTTTGTCTTGCAATACGCTAGAGGTTCCTGTGTTAATGTCAAGAAATGCATTCCACCGGCGAGCTTGCCGGCCAAATTCACGGTTCATGGAATTTGGCCCACGAATATTTCCAAGCCGGTGATCGAGTGTAAAGCTGCACAGAAACTACACCAGTTCAACGAAAAGCTGATAACACCGTCGCTGGAAACCGATCTTCTCCAATCTTGGCCCAGCGTCCGGACAGATAAAGATAATATGACCTTTTGGAAACACGAATATAATAAGCATGGGTCCTGTACTGCGCCAGCTATCACTCAGACAGCGTATTTTGAGAGAGCTCACAAGTTCTGGAAGGAGTATGATCTCTATTCTGTTTTGGAACAAAAAAATATCAAGCCGGGGCAGTCTCGATCGTATTCACTGGCTGATTTTGAAGCAGCCGTCAAGTCCAAGATCGAGAGTGAAGAAACCGAATATCGGCTTGAGTCTCGCGCCCTCTTCTCCCCGACGGACACCATTCCGCTTAGCGTGAAATGTGGGAACGGCCAGAGTTGGCCCACACCCATTTCTTCGGCGAATCCACACACTCCCTCCAAGATTTTGTCGATGTTTGGAAGAGAGAGCATGAGTAGAAGGCGTCCACCAAAGCCCTTCTCATCCGGAGGACTCGGTATGGTCCGACTCCGGAAGGAAGGGTTCAAGGAAGGGTCTCCGGCCTCAAAGAGGCTTTCCTCCCTTCCCGATTTGCTCCGCGAGGCCTACAGAGATTGGCTGCGAAGCTTAAATTCAAGCACGTTCCTCCACGCGGAGGTTCGCCTCAATGGCACTAGCTTGGGCCCGAGTGGCCTGTTCTCCGACACGGGCCGAGTTGCGGCCCATTTCTTCGAAGCGACCAAAGGGTTCATCGAGTTGGGTTTCTTCCGCAACAATCGCATTCGAACGGACGGTCGGCGAACAAGGCAGATACTAGTTGCTATACATATGGCGAAAATACCACCAAGGAAGGGAAGGATTCTCATTCGCAATCAAAAGCGATCTCCTTCGCATGGGGGGCACGCTAAAGTGGTATGCTCGATCACTAGCCCCAGGTTCGTAGCTAAGCACCTCTCCAATTCCACGCACAACAATCTCTCCACTAGTGTTCTTATGAAACGTGAAGTCCGTAAGGATACCAACACCGTCGAGACCGAAGAGTTGTTTTCGTTCCTTCATTTTCAAAATGATGAAGATAATGATGTTGATAGTGTGCATGATGAGCATATCTTTCTTTCTAGTATCCAAGAATTCCATATTCCATTTCCTACGGGTGTGAAGACTCCAAACGAATCACTTATAATTATAGGCCACCGTAATGGGATCATTTGTCTAGCTCAAGCCATTTCGTGTGGAAGGACTCCATATATTGCTCTTTTGGGAAGACAAATCTGTCAGCTATCATCATATGGAATATGGGTGATAGATGAATTTGGTGGTCACAATGGTGGTGCTTGGACAAAACACATAACTTTTGACCTCCTTGTGGAACCGTTGATATTTTGGAAGAGCGACGAGGTTCTTTTGAATGATCCTTACAACACCGATTATAGCGGACTTATATTCTCTTATAATCTAGGTACCAAAAAGCTCAAAAATCTTCCCATTCGAAGCGAGCGGACCGACTCTCTTGCTATTGTGTTTGTGAGCGGTATAGTTTCAATCTTGAGAGGCAACAAACCCAAGAGCAAAGATAATTCTACATCCAATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

604

Amino Acids

68.09

Weight (kDa)

9.43

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 25 - 177 2e-33 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1400, 1671
AasI GACNNNNNNGTC 1 cut(s) 1491
Acc65I GGTACC 1 cut(s) 1679
AccB1I GGYRCC 1 cut(s) 1679
AccBSI CCGCTC 2 cut(s) 1717, 1749
AccII CGCG 3 cut(s) 558, 819, 872
AccIII TCCGGA 3 cut(s) 303, 725, 750
AciI CCGC 8 cut(s) 587, 817, 872, 938, 988, 1653, 1717, 1749
AclWI GGATC 2 cut(s) 1430, 1625
AcoI YGGCCR 2 cut(s) 156, 610
AcsI RAATTY 5 cut(s) 161, 177, 850, 1349, 1529
AcyI GRCGYC 1 cut(s) 703
AfaI GTAC 2 cut(s) 364, 1681
AfiI CCNNNNNNNGG 9 cut(s) 303, 543, 789, 871, 874, 937, 1070, 1112, 1585
AgsI TTSAA 8 cut(s) 52, 244, 500, 766, 855, 1283, 1627, 1761
AhlI ACTAGT 2 cut(s) 1033, 1202
AjnI CCWGG 1 cut(s) 1151
AleI CACNNNNGTG 1 cut(s) 1545
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 4 cut(s) 477, 558, 780, 1244
AlwI GGATC 2 cut(s) 1430, 1625
Ama87I CYCGRG 1 cut(s) 904
Aor13HI TCCGGA 3 cut(s) 303, 725, 750
ApaI GGGCCC 1 cut(s) 905
ApeKI GCWGC 3 cut(s) 221, 503, 838
ApoI RAATTY 5 cut(s) 161, 177, 850, 1349, 1529
ArsI GACNNNNNNTTYG 2 cut(s) 997, 1029
Asp718I GGTACC 1 cut(s) 1679
AspLEI GCGC 2 cut(s) 370, 560
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 2 cut(s) 217, 1531
AsuII TTCGAA 3 cut(s) 951, 1003, 1708
AvaI CYCGRG 1 cut(s) 904
AvaII GGWCC 3 cut(s) 357, 742, 1719
BaeGI GKGCMC 2 cut(s) 905, 1122
BanI GGYRCC 1 cut(s) 1679
BanII GRGCYC 2 cut(s) 406, 905
BbsI GAAGAC 2 cut(s) 1385, 1489
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 3 cut(s) 208, 515, 825
BceAI ACGGC 2 cut(s) 491, 625
BcgI CGANNNNNNTGC 2 cut(s) 1712, 1746
BciT130I CCWGG 1 cut(s) 1153
BciVI GTATCC 2 cut(s) 1228, 1352
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 4 cut(s) 477, 558, 780, 1244
BcuI ACTAGT 2 cut(s) 1033, 1202
BfaI CTAG 8 cut(s) 105, 894, 1034, 1146, 1203, 1338, 1433, 1676
BfmI CTRYAG 2 cut(s) 83, 826
BfuI GTATCC 2 cut(s) 1228, 1352
BglI GCCNNNNNGGC 2 cut(s) 888, 909
BisI GCNGC 4 cut(s) 222, 504, 839, 939
BlpI GCTNAGC 2 cut(s) 590, 1164
BlsI GCNGC 4 cut(s) 223, 505, 840, 940
Bme1390I CCNGG 2 cut(s) 465, 1153
Bme18I GGWCC 3 cut(s) 357, 742, 1719
BmeT110I CYCGRG 1 cut(s) 904
BmiI GGNNCC 5 cut(s) 112, 358, 903, 1590, 1681
BmrFI CCNGG 2 cut(s) 465, 1153
BpiI GAAGAC 2 cut(s) 1385, 1489
Bpu1102I GCTNAGC 2 cut(s) 590, 1164
Bpu14I TTCGAA 3 cut(s) 951, 1003, 1708
BpuEI CTTGAG 3 cut(s) 569, 1422, 1786
BpuMI CCSGG 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 703
BsaI GGTCTC 2 cut(s) 780, 1244
BsaJI CCNNGG 3 cut(s) 464, 1064, 1151
BsaWI WCCGGW 3 cut(s) 303, 725, 750
BsaXI ACNNNNNCTCC 4 cut(s) 641, 671, 1181, 1211
Bsc4I CCNNNNNNNGG 9 cut(s) 303, 543, 789, 871, 874, 937, 1070, 1112, 1585
Bse118I RCCGGY 3 cut(s) 142, 154, 202
Bse1I ACTGG 2 cut(s) 238, 492
BseAI TCCGGA 3 cut(s) 303, 725, 750
BseBI CCWGG 1 cut(s) 1153
BseDI CCNNGG 3 cut(s) 464, 1064, 1151
BseGI GGATG 2 cut(s) 722, 1802
BseLI CCNNNNNNNGG 9 cut(s) 303, 543, 789, 871, 874, 937, 1070, 1112, 1585
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 2 cut(s) 238, 492
BseSI GKGCMC 2 cut(s) 905, 1122
BseXI GCAGC 3 cut(s) 208, 515, 825
BseYI CCCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 3 cut(s) 558, 819, 872
Bsh1285I CGRYCG 2 cut(s) 479, 1015
BshNI GGYRCC 1 cut(s) 1679
BsiEI CGRYCG 2 cut(s) 479, 1015
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 904
BsiSI CCGG 8 cut(s) 143, 155, 203, 304, 464, 726, 751, 779
BslI CCNNNNNNNGG 9 cut(s) 303, 543, 789, 871, 874, 937, 1070, 1112, 1585
BsmAI GTCTC 4 cut(s) 477, 558, 780, 1244
BsmI GAATGC 2 cut(s) 135, 999
Bso31I GGTCTC 2 cut(s) 780, 1244
BsoBI CYCGRG 1 cut(s) 904
Bsp119I TTCGAA 3 cut(s) 951, 1003, 1708
Bsp120I GGGCCC 1 cut(s) 901
Bsp1286I GDGCHC 3 cut(s) 406, 905, 1122
Bsp13I TCCGGA 3 cut(s) 303, 725, 750
Bsp143I GATC 9 cut(s) 207, 277, 427, 476, 519, 1100, 1140, 1422, 1630
Bsp1720I GCTNAGC 2 cut(s) 590, 1164
BspACI CCGC 8 cut(s) 587, 817, 872, 938, 988, 1653, 1717, 1749
BspCNI CTCAG 1 cut(s) 394
BspEI TCCGGA 3 cut(s) 303, 725, 750
BspFNI CGCG 3 cut(s) 558, 819, 872
BspLI GGNNCC 5 cut(s) 112, 358, 903, 1590, 1681
BspPI GGATC 2 cut(s) 1430, 1625
BspQI GCTCTTC 1 cut(s) 1601
BspT104I TTCGAA 3 cut(s) 951, 1003, 1708
BspT107I GGYRCC 1 cut(s) 1679
BspTNI GGTCTC 2 cut(s) 780, 1244
BsrBI CCGCTC 2 cut(s) 1717, 1749
BsrFI RCCGGY 3 cut(s) 142, 154, 202
BsrI ACTGG 2 cut(s) 238, 492
BssAI RCCGGY 3 cut(s) 142, 154, 202
BssECI CCNNGG 3 cut(s) 464, 1064, 1151
BssMI GATC 9 cut(s) 207, 277, 427, 476, 519, 1100, 1140, 1422, 1630
BssNI GRCGYC 1 cut(s) 703
BssT1I CCWWGG 1 cut(s) 1064
Bst2UI CCWGG 1 cut(s) 1153
Bst4CI ACNGT 7 cut(s) 87, 169, 264, 1013, 1246, 1415, 1593
Bst6I CTCTTC 4 cut(s) 569, 676, 1251, 1601
BstACI GRCGYC 1 cut(s) 703
BstBI TTCGAA 3 cut(s) 951, 1003, 1708
BstC8I GCNNGC 6 cut(s) 148, 152, 156, 372, 1122, 1715
BstDEI CTNAG 3 cut(s) 381, 590, 1164
BstENI CCTNNNNNAGG 1 cut(s) 787
BstF5I GGATG 2 cut(s) 722, 1802
BstFNI CGCG 3 cut(s) 558, 819, 872
BstHHI GCGC 2 cut(s) 370, 560
BstKTI GATC 9 cut(s) 210, 280, 430, 479, 522, 1103, 1143, 1425, 1633
BstMAI GTCTC 4 cut(s) 477, 558, 780, 1244
BstMBI GATC 9 cut(s) 207, 277, 427, 476, 519, 1100, 1140, 1422, 1630
BstMCI CGRYCG 2 cut(s) 479, 1015
BstMWI GCNNNNNNNGC 6 cut(s) 888, 909, 996, 1024, 1117, 1321
BstNI CCWGG 1 cut(s) 1153
BstSCI CCNGG 2 cut(s) 463, 1151
BstSFI CTRYAG 2 cut(s) 83, 826
BstSLI GKGCMC 2 cut(s) 905, 1122
BstUI CGCG 3 cut(s) 558, 819, 872
BstV1I GCAGC 3 cut(s) 208, 515, 825
BstV2I GAAGAC 2 cut(s) 1385, 1489
BstXI CCANNNNNNTGG 1 cut(s) 1418
BsuI GTATCC 2 cut(s) 1228, 1352
BtsCI GGATG 2 cut(s) 722, 1802
BtsIMutI CAGTG 1 cut(s) 485
Cac8I GCNNGC 6 cut(s) 148, 152, 156, 372, 1122, 1715
CfoI GCGC 2 cut(s) 370, 560
Cfr10I RCCGGY 3 cut(s) 142, 154, 202
CpoI CGGWCCG 1 cut(s) 1719
CseI GACGC 2 cut(s) 289, 692
Csp6I GTAC 2 cut(s) 363, 1680
CspI CGGWCCG 1 cut(s) 1719
CviAII CATG 6 cut(s) 62, 173, 353, 691, 1112, 1316
CviQI GTAC 2 cut(s) 363, 1680
DdeI CTNAG 3 cut(s) 381, 590, 1164
DpnI GATC 9 cut(s) 209, 279, 429, 478, 521, 1102, 1142, 1424, 1632
DpnII GATC 9 cut(s) 207, 277, 427, 476, 519, 1100, 1140, 1422, 1630
DrdI GACNNNNNNGTC 1 cut(s) 1491
DseDI GACNNNNNNGTC 1 cut(s) 1491
EaeI YGGCCR 2 cut(s) 156, 610
Eam1104I CTCTTC 4 cut(s) 569, 676, 1251, 1601
EarI CTCTTC 4 cut(s) 569, 676, 1251, 1601
Ecl136II GAGCTC 1 cut(s) 404
Eco130I CCWWGG 1 cut(s) 1064
Eco147I AGGCCT 1 cut(s) 824
Eco24I GRGCYC 2 cut(s) 406, 905
Eco31I GGTCTC 2 cut(s) 780, 1244
Eco47I GGWCC 3 cut(s) 357, 742, 1719
Eco53kI GAGCTC 1 cut(s) 404
Eco88I CYCGRG 1 cut(s) 904
EcoICRI GAGCTC 1 cut(s) 404
EcoNI CCTNNNNNAGG 1 cut(s) 787
EcoO109I RGGNCCY 1 cut(s) 357
EcoRI GAATTC 1 cut(s) 1349
EcoRII CCWGG 1 cut(s) 1151
EcoT14I CCWWGG 1 cut(s) 1064
EcoT22I ATGCAT 1 cut(s) 137
EcoT38I GRGCYC 2 cut(s) 406, 905
ErhI CCWWGG 1 cut(s) 1064
FaeI CATG 6 cut(s) 65, 176, 356, 694, 1115, 1319
FatI CATG 6 cut(s) 61, 172, 352, 690, 1111, 1315
FauNDI CATATG 2 cut(s) 1047, 1507
Fnu4HI GCNGC 4 cut(s) 222, 504, 839, 939
FokI GGATG 2 cut(s) 709, 1789
FriOI GRGCYC 2 cut(s) 406, 905
Fsp4HI GCNGC 4 cut(s) 222, 504, 839, 939
FspBI CTAG 8 cut(s) 105, 894, 1034, 1146, 1203, 1338, 1433, 1676
GlaI GCGC 2 cut(s) 369, 559
GluI GCNGC 4 cut(s) 222, 504, 839, 939
GsaI CCCAGC 1 cut(s) 300
HapII CCGG 8 cut(s) 143, 155, 203, 304, 464, 726, 751, 779
HgaI GACGC 2 cut(s) 289, 692
HhaI GCGC 2 cut(s) 370, 560
Hin1I GRCGYC 1 cut(s) 703
Hin1II CATG 6 cut(s) 65, 176, 356, 694, 1115, 1319
Hin6I GCGC 2 cut(s) 368, 558
HinP1I GCGC 2 cut(s) 368, 558
HindIII AAGCTT 1 cut(s) 844
HinfI GANTC 9 cut(s) 551, 643, 732, 747, 1078, 1381, 1391, 1459, 1724
HpaII CCGG 8 cut(s) 143, 155, 203, 304, 464, 726, 751, 779
HphI GGTGA 2 cut(s) 217, 1531
Hpy166II GTNNAC 1 cut(s) 707
Hpy188I TCNGA 3 cut(s) 384, 746, 922
Hpy8I GTNNAC 1 cut(s) 707
Hpy99I CGWCG 4 cut(s) 268, 577, 1250, 1616
HpyCH4III ACNGT 7 cut(s) 87, 169, 264, 1013, 1246, 1415, 1593
HpyCH4IV ACGT 2 cut(s) 860, 1219
HpyCH4V TGCA 5 cut(s) 65, 97, 135, 224, 1315
HpyF10VI GCNNNNNNNGC 6 cut(s) 888, 909, 996, 1024, 1117, 1321
HpyF3I CTNAG 3 cut(s) 381, 590, 1164
HpySE526I ACGT 2 cut(s) 860, 1219
Hsp92I GRCGYC 1 cut(s) 703
Hsp92II CATG 6 cut(s) 65, 176, 356, 694, 1115, 1319
HspAI GCGC 2 cut(s) 368, 558
Kpn2I TCCGGA 3 cut(s) 303, 725, 750
KpnI GGTACC 1 cut(s) 1683
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
Kzo9I GATC 9 cut(s) 207, 277, 427, 476, 519, 1100, 1140, 1422, 1630
LguI GCTCTTC 1 cut(s) 1601
LmnI GCTCC 1 cut(s) 819
Lsp1109I GCAGC 3 cut(s) 208, 515, 825
MaeI CTAG 8 cut(s) 105, 894, 1034, 1146, 1203, 1338, 1433, 1676
MaeII ACGT 2 cut(s) 860, 1219
MaeIII GTNAC 1 cut(s) 1538
MalI GATC 9 cut(s) 209, 279, 429, 478, 521, 1102, 1142, 1424, 1632
MbiI CCGCTC 2 cut(s) 1717, 1749
MboI GATC 9 cut(s) 207, 277, 427, 476, 519, 1100, 1140, 1422, 1630
MhlI GDGCHC 3 cut(s) 406, 905, 1122
MluCI AATT 8 cut(s) 161, 177, 850, 1177, 1349, 1401, 1529, 1795
MlyI GAGTC 6 cut(s) 560, 726, 741, 1375, 1453, 1718
MmeI TCCRAC 2 cut(s) 769, 945
Mph1103I ATGCAT 1 cut(s) 137
MroI TCCGGA 3 cut(s) 303, 725, 750
MroNI GCCGGC 1 cut(s) 154
MseI TTAA 2 cut(s) 120, 848
MslI CAYNNNNRTG 2 cut(s) 1416, 1545
MspI CCGG 8 cut(s) 143, 155, 203, 304, 464, 726, 751, 779
MspR9I CCNGG 2 cut(s) 465, 1153
Mva1269I GAATGC 2 cut(s) 135, 999
MvaI CCWGG 1 cut(s) 1153
MvnI CGCG 3 cut(s) 558, 819, 872
MwoI GCNNNNNNNGC 6 cut(s) 888, 909, 996, 1024, 1117, 1321
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 465
NdeI CATATG 2 cut(s) 1047, 1507
NdeII GATC 9 cut(s) 207, 277, 427, 476, 519, 1100, 1140, 1422, 1630
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 6 cut(s) 65, 176, 356, 694, 1115, 1319
NlaIV GGNNCC 5 cut(s) 112, 358, 903, 1590, 1681
NmeAIII GCCGAG 1 cut(s) 956
NmuCI GTSAC 1 cut(s) 1538
NsiI ATGCAT 1 cut(s) 137
NspV TTCGAA 3 cut(s) 951, 1003, 1708
OliI CACNNNNGTG 1 cut(s) 1545
PceI AGGCCT 1 cut(s) 824
PciSI GCTCTTC 1 cut(s) 1601
PcsI WCGNNNNNNNCGW 1 cut(s) 1225
PctI GAATGC 2 cut(s) 135, 999
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 3 cut(s) 643, 1078, 1391
PkrI GCNGC 4 cut(s) 223, 505, 840, 940
Ple19I CGATCG 1 cut(s) 479
PleI GAGTC 6 cut(s) 559, 726, 741, 1375, 1453, 1718
PpsI GAGTC 6 cut(s) 559, 726, 741, 1375, 1453, 1718
PpuMI RGGWCCY 1 cut(s) 357
PsiI TTATAA 2 cut(s) 1400, 1671
Psp124BI GAGCTC 1 cut(s) 406
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 1151
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 1 cut(s) 1151
PspN4I GGNNCC 5 cut(s) 112, 358, 903, 1590, 1681
PspOMI GGGCCC 1 cut(s) 901
PspPPI RGGWCCY 1 cut(s) 357
PvuI CGATCG 1 cut(s) 479
RsaI GTAC 2 cut(s) 364, 1681
RsaNI GTAC 2 cut(s) 363, 1680
RseI CAYNNNNRTG 2 cut(s) 1416, 1545
Rsr2I CGGWCCG 1 cut(s) 1719
RsrII CGGWCCG 1 cut(s) 1719
SacI GAGCTC 1 cut(s) 406
SapI GCTCTTC 1 cut(s) 1601
SaqAI TTAA 2 cut(s) 120, 848
SatI GCNGC 4 cut(s) 222, 504, 839, 939
Sau3AI GATC 9 cut(s) 207, 277, 427, 476, 519, 1100, 1140, 1422, 1630
SchI GAGTC 6 cut(s) 560, 726, 741, 1375, 1453, 1718
ScrFI CCNGG 2 cut(s) 465, 1153
SduI GDGCHC 3 cut(s) 406, 905, 1122
SfcI CTRYAG 2 cut(s) 83, 826
SfiI GGCCNNNNNGGCC 1 cut(s) 909
SfuI TTCGAA 3 cut(s) 951, 1003, 1708
SgrAI CRCCGGYG 1 cut(s) 142
SinI GGWCC 3 cut(s) 357, 742, 1719
SmiMI CAYNNNNRTG 2 cut(s) 1416, 1545
SmlI CTYRAG 3 cut(s) 548, 1437, 1765
SmoI CTYRAG 3 cut(s) 548, 1437, 1765
SpeI ACTAGT 2 cut(s) 1033, 1202
Sse9I AATT 8 cut(s) 161, 177, 850, 1177, 1349, 1401, 1529, 1795
SseBI AGGCCT 1 cut(s) 824
SsiI CCGC 8 cut(s) 587, 817, 872, 938, 988, 1653, 1717, 1749
SspI AATATT 1 cut(s) 193
SspMI CTAG 8 cut(s) 105, 894, 1034, 1146, 1203, 1338, 1433, 1676
SstI GAGCTC 1 cut(s) 406
StuI AGGCCT 1 cut(s) 824
StyD4I CCNGG 2 cut(s) 463, 1151
StyI CCWWGG 1 cut(s) 1064
TaaI ACNGT 7 cut(s) 87, 169, 264, 1013, 1246, 1415, 1593
TaiI ACGT 2 cut(s) 863, 1222
TaqII GACCGA 2 cut(s) 1268, 1736
TasI AATT 8 cut(s) 161, 177, 850, 1177, 1349, 1401, 1529, 1795
TatI WGTACW 1 cut(s) 362
TauI GCSGC 1 cut(s) 941
TfiI GAWTC 3 cut(s) 643, 1078, 1391
Tru1I TTAA 2 cut(s) 120, 848
Tru9I TTAA 2 cut(s) 120, 848
TscAI CASTG 1 cut(s) 492
TseFI GTSAC 1 cut(s) 1538
TseI GCWGC 3 cut(s) 221, 503, 838
Tsp45I GTSAC 1 cut(s) 1538
TspDTI ATGAA 7 cut(s) 17, 161, 958, 1229, 1265, 1305, 1542
TspGWI ACGGA 3 cut(s) 590, 1022, 1217
TspRI CASTG 1 cut(s) 492
VpaK11BI GGWCC 3 cut(s) 357, 742, 1719
XagI CCTNNNNNAGG 1 cut(s) 787
XapI RAATTY 5 cut(s) 161, 177, 850, 1349, 1529
XspI CTAG 8 cut(s) 105, 894, 1034, 1146, 1203, 1338, 1433, 1676
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.