Rh3AG080700

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000345
Physical Location & Seq
Forward (+)
6462898 .. 6463635
738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG080700.1

Sequence Viewer

Length: 660 bp
ATGGCACAAAATAATATGGCTTACGTCCTCATGACCTGCACTACTGTTGCCTTACTCTTTACTCTGGCCTCCTCGTATACCGCCTACGAATACTTCTTGTTTGTTCAACAGTGGTCCAAGACCGTTTGTGGTAACAGCTGCAAAGCTCCCTCTCCAGTTTTTACGATACATGGGTTGTGGCCCTCCAACCACACTGGCCCTCAGCTGAAGTGCACTGGTGCAGCATATAATCCCACAGAGATGAACAACCAACCCATCTTGAAAAAGAACCTTGAAACTAAATCATGGCCAGATGTTATTCATGGGCAGCATGAGATTTTCTGGATACACGAATGGGGCTTCCACGGCAAATGTTCAGATCTCGTCTTCCCACAGACGAAGTATTTTGAGCGCAGCCATGAAATGTGGACCGATATGGTCATAGGAGATATGCTTAAGAAGGCGTCCATCGTCCCCGCGGCCGGAGCAAAGTATAGCCTTCCCGACATTGAAAAGGCCATCAAAGCCAAGACTTCGCACGACCCATTAATCCGCTGCTTCAAGAATCAGTTGAAGGAAGTAGCATTATGTTACGATTACTATGGCAAAAACGTGGTTGACTGTGATAAATCGCAAAGCAATTGCCCTCAAGCTCCAACTCTTATAGAATATCCGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

219

Amino Acids

24.84

Weight (kDa)

7.57

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 29 - 201 1.8e-38 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 77
AccII CGCG 1 cut(s) 458
AciI CCGC 4 cut(s) 81, 456, 458, 532
AcoI YGGCCR 2 cut(s) 287, 459
AcuI CTGAAG 1 cut(s) 227
AcyI GRCGYC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 461
AflII CTTAAG 1 cut(s) 434
AgsI TTSAA 6 cut(s) 107, 262, 275, 491, 541, 553
AluBI AGCT 4 cut(s) 138, 146, 205, 632
AluI AGCT 4 cut(s) 138, 146, 205, 632
Alw21I GWGCWC 1 cut(s) 215
Alw44I GTGCAC 1 cut(s) 211
AoxI GGCC 6 cut(s) 66, 179, 196, 287, 459, 495
ApaLI GTGCAC 1 cut(s) 211
ApeKI GCWGC 5 cut(s) 138, 221, 307, 393, 534
AseI ATTAAT 1 cut(s) 527
AspLEI GCGC 1 cut(s) 393
AspS9I GGNCC 4 cut(s) 114, 180, 197, 408
AvaII GGWCC 2 cut(s) 114, 408
BaeGI GKGCMC 1 cut(s) 215
BaeI ACNNNNGTAYC 2 cut(s) 158, 191
BalI TGGCCA 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 358
Bbv12I GWGCWC 1 cut(s) 215
BbvCI CCTCAGC 1 cut(s) 201
BbvI GCAGC 5 cut(s) 125, 233, 319, 405, 521
BccI CCATC 3 cut(s) 263, 455, 506
BceAI ACGGC 1 cut(s) 361
BciVI GTATCC 1 cut(s) 318
BfrI CTTAAG 1 cut(s) 434
BfuAI ACCTGC 1 cut(s) 44
BfuI GTATCC 1 cut(s) 318
BglII AGATCT 1 cut(s) 358
BisI GCNGC 6 cut(s) 139, 222, 308, 394, 459, 535
BlsI GCNGC 6 cut(s) 140, 223, 309, 395, 460, 536
Bme18I GGWCC 2 cut(s) 114, 408
BmgT120I GGNCC 4 cut(s) 114, 180, 197, 408
BpiI GAAGAC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 138
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 612
BsaHI GRCGYC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 343, 456
Bsc4I CCNNNNNNNGG 1 cut(s) 461
Bse1I ACTGG 3 cut(s) 155, 199, 220
BseDI CCNNGG 2 cut(s) 343, 456
BseLI CCNNNNNNNGG 1 cut(s) 461
BseMII CTCAG 1 cut(s) 215
BseNI ACTGG 3 cut(s) 155, 199, 220
BseRI GAGGAG 1 cut(s) 61
BseSI GKGCMC 1 cut(s) 215
BseX3I CGGCCG 1 cut(s) 459
BseXI GCAGC 5 cut(s) 125, 233, 319, 405, 521
BsgI GTGCAG 2 cut(s) 22, 240
Bsh1236I CGCG 1 cut(s) 458
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 6 cut(s) 68, 181, 198, 289, 461, 497
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 215
BsiSI CCGG 1 cut(s) 462
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 6 cut(s) 68, 181, 198, 289, 461, 497
Bsp1286I GDGCHC 1 cut(s) 215
Bsp143I GATC 1 cut(s) 358
BspACI CCGC 4 cut(s) 81, 456, 458, 532
BspANI GGCC 6 cut(s) 68, 181, 198, 289, 461, 497
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 1 cut(s) 458
BspHI TCATGA 1 cut(s) 30
BspMI ACCTGC 1 cut(s) 44
BspTI CTTAAG 1 cut(s) 434
BsrI ACTGG 3 cut(s) 155, 199, 220
BssECI CCNNGG 2 cut(s) 343, 456
BssMI GATC 1 cut(s) 358
BssNAI GTATAC 1 cut(s) 78
BssNI GRCGYC 1 cut(s) 443
Bst1107I GTATAC 1 cut(s) 78
Bst4CI ACNGT 4 cut(s) 46, 111, 124, 602
BstACI GRCGYC 1 cut(s) 443
BstAFI CTTAAG 1 cut(s) 434
BstDEI CTNAG 1 cut(s) 201
BstDSI CCRYGG 2 cut(s) 343, 456
BstFNI CGCG 1 cut(s) 458
BstHHI GCGC 1 cut(s) 393
BstKTI GATC 1 cut(s) 361
BstMBI GATC 1 cut(s) 358
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 3 cut(s) 345, 464, 503
BstSLI GKGCMC 1 cut(s) 215
BstUI CGCG 1 cut(s) 458
BstV1I GCAGC 5 cut(s) 125, 233, 319, 405, 521
BstV2I GAAGAC 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BstZ17I GTATAC 1 cut(s) 78
BstZI CGGCCG 1 cut(s) 459
BsuI GTATCC 1 cut(s) 318
BsuRI GGCC 6 cut(s) 68, 181, 198, 289, 461, 497
BtgI CCRYGG 2 cut(s) 343, 456
BtsIMutI CAGTG 3 cut(s) 116, 192, 213
BveI ACCTGC 1 cut(s) 44
CciI TCATGA 1 cut(s) 30
CfoI GCGC 1 cut(s) 393
Cfr13I GGNCC 4 cut(s) 114, 180, 197, 408
Cfr42I CCGCGG 1 cut(s) 459
CseI GACGC 1 cut(s) 432
CviAII CATG 6 cut(s) 31, 170, 285, 302, 311, 398
DdeI CTNAG 1 cut(s) 201
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
EaeI YGGCCR 2 cut(s) 287, 459
EagI CGGCCG 1 cut(s) 459
EclXI CGGCCG 1 cut(s) 459
Eco47I GGWCC 2 cut(s) 114, 408
Eco52I CGGCCG 1 cut(s) 459
Eco57I CTGAAG 1 cut(s) 227
FaeI CATG 6 cut(s) 34, 173, 288, 305, 314, 401
FaqI GGGAC 1 cut(s) 437
FatI CATG 6 cut(s) 30, 169, 284, 301, 310, 397
FauI CCCGC 1 cut(s) 463
FblI GTMKAC 1 cut(s) 77
Fnu4HI GCNGC 6 cut(s) 139, 222, 308, 394, 459, 535
Fsp4HI GCNGC 6 cut(s) 139, 222, 308, 394, 459, 535
GlaI GCGC 1 cut(s) 392
GluI GCNGC 6 cut(s) 139, 222, 308, 394, 459, 535
GsuI CTGGAG 1 cut(s) 138
HaeIII GGCC 6 cut(s) 68, 181, 198, 289, 461, 497
HapII CCGG 1 cut(s) 462
HgaI GACGC 1 cut(s) 432
HhaI GCGC 1 cut(s) 393
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 6 cut(s) 34, 173, 288, 305, 314, 401
Hin6I GCGC 1 cut(s) 391
HinP1I GCGC 1 cut(s) 391
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HinfI GANTC 1 cut(s) 544
HpaII CCGG 1 cut(s) 462
Hpy166II GTNNAC 4 cut(s) 78, 213, 408, 598
Hpy188I TCNGA 2 cut(s) 358, 654
Hpy188III TCNNGA 5 cut(s) 31, 259, 322, 482, 541
Hpy8I GTNNAC 4 cut(s) 78, 213, 408, 598
HpyAV CCTTC 3 cut(s) 433, 488, 547
HpyCH4III ACNGT 4 cut(s) 46, 111, 124, 602
HpyCH4IV ACGT 2 cut(s) 24, 591
HpyCH4V TGCA 4 cut(s) 39, 141, 213, 221
HpyF10VI GCNNNNNNNGC 3 cut(s) 345, 464, 503
HpyF3I CTNAG 1 cut(s) 201
HpySE526I ACGT 2 cut(s) 24, 591
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 6 cut(s) 34, 173, 288, 305, 314, 401
HspAI GCGC 1 cut(s) 391
KspI CCGCGG 1 cut(s) 459
Kzo9I GATC 1 cut(s) 358
LmnI GCTCC 3 cut(s) 151, 464, 637
LpnPI CCDG 8 cut(s) 49, 50, 168, 180, 201, 303, 307, 475
Lsp1109I GCAGC 5 cut(s) 125, 233, 319, 405, 521
MaeII ACGT 2 cut(s) 24, 591
MaeIII GTNAC 2 cut(s) 131, 569
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MboII GAAGA 1 cut(s) 358
MfeI CAATTG 1 cut(s) 619
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 289
MluCI AATT 1 cut(s) 619
MluNI TGGCCA 1 cut(s) 289
MmeI TCCRAC 2 cut(s) 210, 659
MnlI CCTC 7 cut(s) 38, 79, 82, 160, 193, 210, 636
Mox20I TGGCCA 1 cut(s) 289
MscI TGGCCA 1 cut(s) 289
MseI TTAA 2 cut(s) 435, 527
MslI CAYNNNNRTG 1 cut(s) 239
Msp20I TGGCCA 1 cut(s) 289
MspA1I CMGCKG 4 cut(s) 138, 205, 458, 534
MspCI CTTAAG 1 cut(s) 434
MspI CCGG 1 cut(s) 462
MunI CAATTG 1 cut(s) 619
MvnI CGCG 1 cut(s) 458
MwoI GCNNNNNNNGC 3 cut(s) 345, 464, 503
NdeII GATC 1 cut(s) 358
NlaIII CATG 6 cut(s) 34, 173, 288, 305, 314, 401
PagI TCATGA 1 cut(s) 30
PfeI GAWTC 1 cut(s) 544
PkrI GCNGC 6 cut(s) 140, 223, 309, 395, 460, 536
PshBI ATTAAT 1 cut(s) 527
PspPI GGNCC 4 cut(s) 114, 180, 197, 408
PsuI RGATCY 1 cut(s) 358
PvuII CAGCTG 2 cut(s) 138, 205
RseI CAYNNNNRTG 1 cut(s) 239
SacII CCGCGG 1 cut(s) 459
SaqAI TTAA 2 cut(s) 435, 527
SatI GCNGC 6 cut(s) 139, 222, 308, 394, 459, 535
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 4 cut(s) 114, 180, 197, 408
SduI GDGCHC 1 cut(s) 215
SetI ASST 8 cut(s) 27, 38, 140, 148, 207, 273, 594, 634
Sfr303I CCGCGG 1 cut(s) 459
SgrBI CCGCGG 1 cut(s) 459
SinI GGWCC 2 cut(s) 114, 408
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 434, 627
SmoI CTYRAG 2 cut(s) 434, 627
Sse9I AATT 1 cut(s) 619
SsiI CCGC 4 cut(s) 81, 456, 458, 532
TaaI ACNGT 4 cut(s) 46, 111, 124, 602
TaiI ACGT 2 cut(s) 27, 594
TaqII GACCGA 1 cut(s) 425
TasI AATT 1 cut(s) 619
TauI GCSGC 1 cut(s) 461
TfiI GAWTC 1 cut(s) 544
Tru1I TTAA 2 cut(s) 435, 527
Tru9I TTAA 2 cut(s) 435, 527
TscAI CASTG 3 cut(s) 116, 199, 220
TseI GCWGC 5 cut(s) 138, 221, 307, 393, 534
TspDTI ATGAA 3 cut(s) 257, 290, 414
TspRI CASTG 3 cut(s) 116, 199, 220
Vha464I CTTAAG 1 cut(s) 434
VneI GTGCAC 1 cut(s) 211
VpaK11BI GGWCC 2 cut(s) 114, 408
VspI ATTAAT 1 cut(s) 527
XmiI GTMKAC 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.