Rorug03G0028600

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000345
Physical Location & Seq
Forward (+)
2280365 .. 2281602
1238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0028600.1

Sequence Viewer

Length: 765 bp
ATGAACAATAATCAGGATATGCATGAGGATGACTACTACTCGATCAAAGCTGCAAATCTTCATGAGCCCGCTGCAGAATTAGCATTCACTCCCAAGCTTACCGCTACTTCCAACATGACCCAAAGTTATGTGTTATCATTCAACCACCACCAGCATGATCAGGCGGTTGGAGTGACGTCGTCACAAGGCCGAAGTGGCGGTGATTATTGCTATAATGGTCACCAACCCTCCTCTGCAGCTTCGCAGCCAAGTTCCCAATGGAATCCAACCCCAGAGCAGTTACGGGTTCTGGATGAGCTGTACGGACATGGATTAAAGACACCGACGGCACAGCAAATCCGACAAGTGACTGCACGGCTTCGACACTTTGGAAAGATTGAAGGCAAGAATGTCTTCTACTGGTTTCAGAACCACCGAGCGAGGGAGAGGCAGAAGAGACGAAGAGAACTCATGTCCATGCGTCAGTATCACTCTAAGGAACAACAACTTTGGAGTAACAAAGAATCTGCAGGAGCTGCAGGACTGAGAAGGTCAAGTACTCTTGAAATTGAGCACCAGAATAAATGGACACTATCAAACTGCGGTGTTCTGCTTGCTGATCAGGAATTATCTCCAGGGTTGCGGAAGAGGATGAGCATGGTGACGGGTATTTACTCCGGTCAATTGGAGGAGCAATCCTATTCGATCGGCTACACAGACCTCGGAAGCACGCGCAGCTACATTAATATACCCAAAGCTCTTGGACTATCATCATCCCCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

254

Amino Acids

28.75

Weight (kDa)

9.15

Isoelectric Point (pI)

65.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 85 - 144 1.9e-12 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 179
AccII CGCG 1 cut(s) 712
AciI CCGC 6 cut(s) 69, 102, 164, 198, 582, 622
AcyI GRCGYC 1 cut(s) 176
AfaI GTAC 2 cut(s) 302, 538
AfiI CCNNNNNNNGG 1 cut(s) 421
AgsI TTSAA 3 cut(s) 142, 380, 545
AjnI CCWGG 1 cut(s) 613
AluBI AGCT 7 cut(s) 50, 97, 239, 298, 515, 717, 737
AluI AGCT 7 cut(s) 50, 97, 239, 298, 515, 717, 737
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 1 cut(s) 430
AlwNI CAGNNNCTG 1 cut(s) 515
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 6 cut(s) 50, 71, 236, 244, 515, 714
AseI ATTAAT 1 cut(s) 723
Asp700I GAANNNNTTC 1 cut(s) 392
AspLEI GCGC 1 cut(s) 714
AsuHPI GGTGA 3 cut(s) 212, 212, 652
BanII GRGCYC 1 cut(s) 69
BarI GAAGNNNNNNTAC 2 cut(s) 520, 552
BbsI GAAGAC 1 cut(s) 385
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 6 cut(s) 37, 58, 248, 256, 502, 726
BceAI ACGGC 2 cut(s) 342, 371
BciT130I CCWGG 1 cut(s) 615
BclI TGATCA 2 cut(s) 157, 598
BcoDI GTCTC 1 cut(s) 430
BfmI CTRYAG 4 cut(s) 72, 234, 507, 516
BglI GCCNNNNNGGC 1 cut(s) 195
BisI GCNGC 6 cut(s) 51, 72, 237, 245, 516, 715
BlsI GCNGC 6 cut(s) 52, 73, 238, 246, 517, 716
BmcAI AGTACT 1 cut(s) 538
Bme1390I CCNGG 1 cut(s) 615
BmrFI CCNGG 1 cut(s) 615
BpiI GAAGAC 1 cut(s) 385
BpmI CTGGAG 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 614, 700
BsaWI WCCGGW 1 cut(s) 656
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 404
BseBI CCWGG 1 cut(s) 615
BseDI CCNNGG 2 cut(s) 614, 700
BseGI GGATG 4 cut(s) 34, 298, 636, 752
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMII CTCAG 1 cut(s) 515
BseNI ACTGG 1 cut(s) 404
BseRI GAGGAG 2 cut(s) 220, 683
BseXI GCAGC 6 cut(s) 37, 58, 248, 256, 502, 726
BsgI GTGCAG 1 cut(s) 336
Bsh1236I CGCG 1 cut(s) 712
Bsh1285I CGRYCG 1 cut(s) 687
BshFI GGCC 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 687
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 1 cut(s) 657
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 1 cut(s) 430
BsmBI CGTCTC 1 cut(s) 430
BsmI GAATGC 1 cut(s) 83
BsnI GGCC 1 cut(s) 189
Bsp1286I GDGCHC 2 cut(s) 69, 555
Bsp143I GATC 4 cut(s) 42, 157, 598, 684
BspACI CCGC 6 cut(s) 69, 102, 164, 198, 582, 622
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 1 cut(s) 516
BspFNI CGCG 1 cut(s) 712
BspHI TCATGA 1 cut(s) 61
BspMAI CTGCAG 4 cut(s) 76, 238, 511, 520
BsrI ACTGG 1 cut(s) 404
BssECI CCNNGG 2 cut(s) 614, 700
BssMI GATC 4 cut(s) 42, 157, 598, 684
BssNI GRCGYC 1 cut(s) 176
Bst2UI CCWGG 1 cut(s) 615
Bst6I CTCTTC 3 cut(s) 428, 436, 620
BstACI GRCGYC 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 69, 594, 710
BstDEI CTNAG 2 cut(s) 474, 524
BstEII GGTNACC 1 cut(s) 218
BstF5I GGATG 4 cut(s) 34, 298, 636, 752
BstFNI CGCG 1 cut(s) 712
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 4 cut(s) 45, 160, 601, 687
BstMAI GTCTC 1 cut(s) 430
BstMBI GATC 4 cut(s) 42, 157, 598, 684
BstMCI CGRYCG 1 cut(s) 687
BstMWI GCNNNNNNNGC 4 cut(s) 80, 195, 515, 714
BstNI CCWGG 1 cut(s) 615
BstPI GGTNACC 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 613
BstSFI CTRYAG 4 cut(s) 72, 234, 507, 516
BstUI CGCG 1 cut(s) 712
BstV1I GCAGC 6 cut(s) 37, 58, 248, 256, 502, 726
BstV2I GAAGAC 1 cut(s) 385
BsuRI GGCC 1 cut(s) 189
BtsCI GGATG 4 cut(s) 34, 298, 636, 752
Cac8I GCNNGC 3 cut(s) 69, 594, 710
CaiI CAGNNNCTG 1 cut(s) 515
CciI TCATGA 1 cut(s) 61
CfoI GCGC 1 cut(s) 714
CseI GACGC 1 cut(s) 449
Csp6I GTAC 2 cut(s) 301, 537
CviAII CATG 8 cut(s) 23, 62, 115, 155, 308, 451, 457, 637
CviQI GTAC 2 cut(s) 301, 537
DdeI CTNAG 2 cut(s) 474, 524
DpnI GATC 4 cut(s) 44, 159, 600, 686
DpnII GATC 4 cut(s) 42, 157, 598, 684
Eam1104I CTCTTC 3 cut(s) 428, 436, 620
EarI CTCTTC 3 cut(s) 428, 436, 620
Eco24I GRGCYC 1 cut(s) 69
Eco91I GGTNACC 1 cut(s) 218
EcoO65I GGTNACC 1 cut(s) 218
EcoRII CCWGG 1 cut(s) 613
EcoT22I ATGCAT 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 430
FaeI CATG 8 cut(s) 26, 65, 118, 158, 311, 454, 460, 640
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FatI CATG 8 cut(s) 22, 61, 114, 154, 307, 450, 456, 636
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 2 cut(s) 157, 598
Fnu4HI GCNGC 6 cut(s) 51, 72, 237, 245, 516, 715
FokI GGATG 4 cut(s) 41, 305, 643, 739
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 6 cut(s) 51, 72, 237, 245, 516, 715
GlaI GCGC 1 cut(s) 713
GluI GCNGC 6 cut(s) 51, 72, 237, 245, 516, 715
GsuI CTGGAG 1 cut(s) 597
HaeIII GGCC 1 cut(s) 189
HapII CCGG 1 cut(s) 657
HgaI GACGC 1 cut(s) 449
HhaI GCGC 1 cut(s) 714
Hin1I GRCGYC 1 cut(s) 176
Hin1II CATG 8 cut(s) 26, 65, 118, 158, 311, 454, 460, 640
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 2 cut(s) 262, 503
HpaII CCGG 1 cut(s) 657
HphI GGTGA 3 cut(s) 212, 212, 652
Hpy188I TCNGA 3 cut(s) 341, 408, 704
Hpy188III TCNNGA 5 cut(s) 14, 62, 290, 542, 602
Hpy99I CGWCG 2 cut(s) 181, 328
HpyAV CCTTC 2 cut(s) 374, 522
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 7 cut(s) 22, 53, 74, 236, 353, 509, 518
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 195, 515, 714
HpyF3I CTNAG 2 cut(s) 474, 524
HpySE526I ACGT 1 cut(s) 176
Hsp92I GRCGYC 1 cut(s) 176
Hsp92II CATG 8 cut(s) 26, 65, 118, 158, 311, 454, 460, 640
HspAI GCGC 1 cut(s) 712
Ksp22I TGATCA 2 cut(s) 157, 598
Kzo9I GATC 4 cut(s) 42, 157, 598, 684
LmnI GCTCC 2 cut(s) 512, 670
Lsp1109I GCAGC 6 cut(s) 37, 58, 248, 256, 502, 726
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 7 cut(s) 172, 180, 218, 279, 346, 494, 640
MalI GATC 4 cut(s) 44, 159, 600, 686
MboI GATC 4 cut(s) 42, 157, 598, 684
MboII GAAGA 5 cut(s) 50, 385, 445, 453, 637
MfeI CAATTG 1 cut(s) 662
MhlI GDGCHC 2 cut(s) 69, 555
MluCI AATT 4 cut(s) 77, 546, 605, 662
MmeI TCCRAC 4 cut(s) 135, 148, 290, 364
MnlI CCTC 8 cut(s) 19, 238, 241, 414, 420, 621, 661, 710
Mph1103I ATGCAT 1 cut(s) 24
MroXI GAANNNNTTC 1 cut(s) 392
MseI TTAA 2 cut(s) 314, 723
MslI CAYNNNNRTG 3 cut(s) 27, 153, 455
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 657
MspR9I CCNGG 1 cut(s) 615
MunI CAATTG 1 cut(s) 662
Mva1269I GAATGC 1 cut(s) 83
MvaI CCWGG 1 cut(s) 615
MvnI CGCG 1 cut(s) 712
MwoI GCNNNNNNNGC 4 cut(s) 80, 195, 515, 714
NdeII GATC 4 cut(s) 42, 157, 598, 684
NlaIII CATG 8 cut(s) 26, 65, 118, 158, 311, 454, 460, 640
NmuCI GTSAC 5 cut(s) 172, 180, 218, 346, 640
NsiI ATGCAT 1 cut(s) 24
PagI TCATGA 1 cut(s) 61
PctI GAATGC 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 392
PfeI GAWTC 2 cut(s) 262, 503
PflFI GACNNNGTC 1 cut(s) 178
PkrI GCNGC 6 cut(s) 52, 73, 238, 246, 517, 716
Ple19I CGATCG 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 613
PspEI GGTNACC 1 cut(s) 218
PspGI CCWGG 1 cut(s) 613
PstI CTGCAG 4 cut(s) 76, 238, 511, 520
PstNI CAGNNNCTG 1 cut(s) 515
PsyI GACNNNGTC 1 cut(s) 178
PvuI CGATCG 1 cut(s) 687
RsaI GTAC 2 cut(s) 302, 538
RsaNI GTAC 2 cut(s) 301, 537
RseI CAYNNNNRTG 3 cut(s) 27, 153, 455
SaqAI TTAA 2 cut(s) 314, 723
SatI GCNGC 6 cut(s) 51, 72, 237, 245, 516, 715
Sau3AI GATC 4 cut(s) 42, 157, 598, 684
ScaI AGTACT 1 cut(s) 538
ScrFI CCNGG 1 cut(s) 615
SduI GDGCHC 2 cut(s) 69, 555
SfcI CTRYAG 4 cut(s) 72, 234, 507, 516
SmiMI CAYNNNNRTG 3 cut(s) 27, 153, 455
Sse9I AATT 4 cut(s) 77, 546, 605, 662
SsiI CCGC 6 cut(s) 69, 102, 164, 198, 582, 622
StyD4I CCNGG 1 cut(s) 613
TaiI ACGT 1 cut(s) 179
TaqI TCGA 3 cut(s) 41, 361, 683
TasI AATT 4 cut(s) 77, 546, 605, 662
TatI WGTACW 1 cut(s) 536
TfiI GAWTC 2 cut(s) 262, 503
Tru1I TTAA 2 cut(s) 314, 723
Tru9I TTAA 2 cut(s) 314, 723
TseFI GTSAC 5 cut(s) 172, 180, 218, 346, 640
TseI GCWGC 6 cut(s) 50, 71, 236, 244, 515, 714
Tsp45I GTSAC 5 cut(s) 172, 180, 218, 346, 640
TspDTI ATGAA 2 cut(s) 17, 50
TspGWI ACGGA 1 cut(s) 318
Tth111I GACNNNGTC 1 cut(s) 178
VspI ATTAAT 1 cut(s) 723
XcmI CCANNNNNNNNNTGG 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 392
ZraI GACGTC 1 cut(s) 177
ZrmI AGTACT 1 cut(s) 538
Zsp2I ATGCAT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.