MD15G1167100.v1.1

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000345
Physical Location & Seq
Reverse (-)
12597982 .. 12598817
836 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1167100.v1.1.491

Sequence Viewer

Length: 669 bp
ATGTTCAGTTCTGGCCAGCTGCTGAATGCCAAGCTACAGGGGGGGGTGAATAAATGCCACATCATGCAACCATCTCATCTTCAGTTCACTATTCATGGTCTCTGGCCTGCGAATAAATCATCCTCTTCATCCCTTGGCTGCAAAGGGGATGCCTTTAATGTGAATGAGATGGATGATGCACTGAAAAAGGCGCTACTTAGTTCGTGGTGGGATTGGAGAAAAGGGAAGCCTGTCGAATTCTGGCAACGAGAGTATGATAAGCACGGAAAGTGCTCCGACAATGTGTTCCTGAAGACCGAGTACTTCCGGAAGACCCTAGCGATGTACCATGATTTTGATATAGCTCAAATCTTGCAGAAGGATAATATTGTACCCCAACCACTTCAGCCCAAGATGTCGTTGTACAAATTGTACTCCATCGATCAGATAACAAAGGCAATTGAGTCCGAAACCGGCGGACATTCCGTCAATATCAGATGCTATCAACAATACCAGAAGAAGAGCAACAATGGAAGTAAACCATTAATATATTTGGCCCAAGTCGCCCTCTGCTATAATAGATCTGGCAATAACAGAACTAACTGTGATCCAGGCGAAACAACCAGCTGCAACTTAAATAAATCAGAACCGAAAACTGTACCACGTATAGCCCACATAAAGCTCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

223

Amino Acids

25.36

Weight (kDa)

9.24

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 18 - 195 2.2e-30 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 306
AciI CCGC 1 cut(s) 456
AclWI GGATC 1 cut(s) 581
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 1 cut(s) 236
AcuI CTGAAG 3 cut(s) 65, 311, 368
AfaI GTAC 6 cut(s) 302, 326, 372, 404, 413, 639
AhdI GACNNNNNGTC 1 cut(s) 464
AjnI CCWGG 1 cut(s) 589
AluBI AGCT 5 cut(s) 19, 34, 344, 606, 661
AluI AGCT 5 cut(s) 19, 34, 344, 606, 661
Alw21I GWGCWC 1 cut(s) 275
Alw26I GTCTC 1 cut(s) 104
AlwI GGATC 1 cut(s) 581
AlwNI CAGNNNCTG 1 cut(s) 22
Aor13HI TCCGGA 1 cut(s) 306
AoxI GGCC 3 cut(s) 13, 104, 534
ApeKI GCWGC 3 cut(s) 19, 138, 606
ApoI RAATTY 1 cut(s) 236
AseI ATTAAT 1 cut(s) 524
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 1 cut(s) 535
AsuHPI GGTGA 1 cut(s) 58
BalI TGGCCA 1 cut(s) 15
BarI GAAGNNNNNNTAC 2 cut(s) 284, 316
BbsI GAAGAC 2 cut(s) 299, 317
Bbv12I GWGCWC 1 cut(s) 275
BbvI GCAGC 3 cut(s) 6, 125, 593
BccI CCATC 3 cut(s) 79, 163, 425
BciT130I CCWGG 1 cut(s) 591
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 194
BglII AGATCT 1 cut(s) 560
BisI GCNGC 3 cut(s) 20, 139, 607
BlsI GCNGC 3 cut(s) 21, 140, 608
BmcAI AGTACT 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 591
BmeRI GACNNNNNGTC 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 535
BmrFI CCNGG 1 cut(s) 591
BmsI GCATC 3 cut(s) 139, 166, 467
BpiI GAAGAC 2 cut(s) 299, 317
Bsa29I ATCGAT 1 cut(s) 420
BsaAI YACGTR 1 cut(s) 644
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 1 cut(s) 133
BsaWI WCCGGW 1 cut(s) 306
Bse118I RCCGGY 1 cut(s) 452
BseAI TCCGGA 1 cut(s) 306
BseBI CCWGG 1 cut(s) 591
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 133
BseGI GGATG 4 cut(s) 119, 128, 154, 178
BseXI GCAGC 3 cut(s) 6, 125, 593
BshFI GGCC 3 cut(s) 15, 106, 536
BshVI ATCGAT 1 cut(s) 420
BsiHKAI GWGCWC 1 cut(s) 275
BsiSI CCGG 2 cut(s) 307, 453
BsmAI GTCTC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 31
BsnI GGCC 3 cut(s) 15, 106, 536
Bso31I GGTCTC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 275
Bsp13I TCCGGA 1 cut(s) 306
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 3 cut(s) 421, 560, 586
BspACI CCGC 1 cut(s) 456
BspANI GGCC 3 cut(s) 15, 106, 536
BspDI ATCGAT 1 cut(s) 420
BspEI TCCGGA 1 cut(s) 306
BspPI GGATC 1 cut(s) 581
BspQI GCTCTTC 1 cut(s) 494
BspTNI GGTCTC 1 cut(s) 104
BsrFI RCCGGY 1 cut(s) 452
BsrGI TGTACA 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 452
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 3 cut(s) 421, 560, 586
BssT1I CCWWGG 1 cut(s) 133
Bst2UI CCWGG 1 cut(s) 591
Bst4CI ACNGT 2 cut(s) 584, 637
Bst6I CTCTTC 2 cut(s) 130, 494
BstAUI TGTACA 1 cut(s) 402
BstBAI YACGTR 1 cut(s) 644
BstC8I GCNNGC 2 cut(s) 17, 108
BstDEI CTNAG 1 cut(s) 197
BstF5I GGATG 4 cut(s) 119, 128, 154, 178
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 3 cut(s) 424, 563, 589
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 3 cut(s) 421, 560, 586
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 1 cut(s) 591
BstSCI CCNGG 1 cut(s) 589
BstSFI CTRYAG 1 cut(s) 35
BstV1I GCAGC 3 cut(s) 6, 125, 593
BstV2I GAAGAC 2 cut(s) 299, 317
BstX2I RGATCY 1 cut(s) 560
BstYI RGATCY 1 cut(s) 560
Bsu15I ATCGAT 1 cut(s) 420
BsuRI GGCC 3 cut(s) 15, 106, 536
BsuTUI ATCGAT 1 cut(s) 420
BtgZI GCGATG 1 cut(s) 335
BtsCI GGATG 4 cut(s) 119, 128, 154, 178
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 17, 108
CaiI CAGNNNCTG 1 cut(s) 22
CfoI GCGC 1 cut(s) 193
Cfr10I RCCGGY 1 cut(s) 452
Cfr13I GGNCC 1 cut(s) 535
ClaI ATCGAT 1 cut(s) 420
Csp6I GTAC 6 cut(s) 301, 325, 371, 403, 412, 638
CviAII CATG 3 cut(s) 64, 95, 329
CviQI GTAC 6 cut(s) 301, 325, 371, 403, 412, 638
DdeI CTNAG 1 cut(s) 197
DpnI GATC 3 cut(s) 423, 562, 588
DpnII GATC 3 cut(s) 421, 560, 586
DriI GACNNNNNGTC 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 2 cut(s) 130, 494
Eam1105I GACNNNNNGTC 1 cut(s) 464
EarI CTCTTC 2 cut(s) 130, 494
EciI GGCGGA 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 104
Eco57I CTGAAG 3 cut(s) 65, 311, 368
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 133
FaeI CATG 3 cut(s) 67, 98, 332
FaiI YATR 9 cut(s) 65, 96, 255, 330, 341, 529, 555, 647, 656
FatI CATG 3 cut(s) 63, 94, 328
Fnu4HI GCNGC 3 cut(s) 20, 139, 607
FokI GGATG 4 cut(s) 106, 115, 161, 185
Fsp4HI GCNGC 3 cut(s) 20, 139, 607
FspBI CTAG 1 cut(s) 317
GlaI GCGC 1 cut(s) 192
GluI GCNGC 3 cut(s) 20, 139, 607
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 3 cut(s) 15, 106, 536
HapII CCGG 2 cut(s) 307, 453
HhaI GCGC 1 cut(s) 193
Hin1II CATG 3 cut(s) 67, 98, 332
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HinfI GANTC 1 cut(s) 443
HpaII CCGG 2 cut(s) 307, 453
HphI GGTGA 1 cut(s) 58
Hpy166II GTNNAC 2 cut(s) 87, 518
Hpy188I TCNGA 5 cut(s) 277, 426, 448, 476, 625
Hpy188III TCNNGA 2 cut(s) 289, 307
Hpy8I GTNNAC 2 cut(s) 87, 518
HpyAV CCTTC 1 cut(s) 352
HpyCH4III ACNGT 2 cut(s) 584, 637
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 5 cut(s) 67, 141, 179, 355, 609
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 1 cut(s) 643
Hsp92II CATG 3 cut(s) 67, 98, 332
HspAI GCGC 1 cut(s) 191
Kpn2I TCCGGA 1 cut(s) 306
Kzo9I GATC 3 cut(s) 421, 560, 586
LguI GCTCTTC 1 cut(s) 494
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 3 cut(s) 6, 125, 593
LweI GCATC 3 cut(s) 139, 166, 467
MaeI CTAG 1 cut(s) 317
MaeII ACGT 1 cut(s) 643
MalI GATC 3 cut(s) 423, 562, 588
MboI GATC 3 cut(s) 421, 560, 586
MboII GAAGA 6 cut(s) 71, 117, 304, 322, 508, 511
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 1 cut(s) 560
MhlI GDGCHC 1 cut(s) 275
MlsI TGGCCA 1 cut(s) 15
MluCI AATT 3 cut(s) 236, 407, 438
MluNI TGGCCA 1 cut(s) 15
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 2 cut(s) 133, 557
Mox20I TGGCCA 1 cut(s) 15
MroI TCCGGA 1 cut(s) 306
MscI TGGCCA 1 cut(s) 15
MseI TTAA 3 cut(s) 156, 524, 614
Msp20I TGGCCA 1 cut(s) 15
MspA1I CMGCKG 2 cut(s) 19, 606
MspI CCGG 2 cut(s) 307, 453
MspR9I CCNGG 1 cut(s) 591
MunI CAATTG 1 cut(s) 438
Mva1269I GAATGC 1 cut(s) 31
MvaI CCWGG 1 cut(s) 591
MwoI GCNNNNNNNGC 1 cut(s) 542
NdeII GATC 3 cut(s) 421, 560, 586
NlaIII CATG 3 cut(s) 67, 98, 332
PciSI GCTCTTC 1 cut(s) 494
PctI GAATGC 1 cut(s) 31
PkrI GCNGC 3 cut(s) 21, 140, 608
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
Ppu21I YACGTR 1 cut(s) 644
PshBI ATTAAT 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 589
PspGI CCWGG 1 cut(s) 589
PspPI GGNCC 1 cut(s) 535
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 1 cut(s) 560
PvuII CAGCTG 2 cut(s) 19, 606
RsaI GTAC 6 cut(s) 302, 326, 372, 404, 413, 639
RsaNI GTAC 6 cut(s) 301, 325, 371, 403, 412, 638
SapI GCTCTTC 1 cut(s) 494
SaqAI TTAA 3 cut(s) 156, 524, 614
SatI GCNGC 3 cut(s) 20, 139, 607
Sau3AI GATC 3 cut(s) 421, 560, 586
Sau96I GGNCC 1 cut(s) 535
ScaI AGTACT 1 cut(s) 302
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 1 cut(s) 591
SduI GDGCHC 1 cut(s) 275
SetI ASST 6 cut(s) 21, 36, 346, 608, 646, 663
SfaNI GCATC 3 cut(s) 139, 166, 467
SfcI CTRYAG 1 cut(s) 35
Sse9I AATT 3 cut(s) 236, 407, 438
SsiI CCGC 1 cut(s) 456
SspI AATATT 1 cut(s) 367
SspMI CTAG 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 589
StyI CCWWGG 1 cut(s) 133
TaaI ACNGT 2 cut(s) 584, 637
TaiI ACGT 1 cut(s) 646
TaqI TCGA 2 cut(s) 234, 420
TaqII GACCGA 1 cut(s) 311
TasI AATT 3 cut(s) 236, 407, 438
TatI WGTACW 3 cut(s) 300, 402, 411
Tru1I TTAA 3 cut(s) 156, 524, 614
Tru9I TTAA 3 cut(s) 156, 524, 614
TscAI CASTG 1 cut(s) 186
TseI GCWGC 3 cut(s) 19, 138, 606
TspDTI ATGAA 2 cut(s) 83, 117
TspGWI ACGGA 2 cut(s) 279, 454
TspRI CASTG 1 cut(s) 186
VspI ATTAAT 1 cut(s) 524
XapI RAATTY 1 cut(s) 236
XspI CTAG 1 cut(s) 317
ZrmI AGTACT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.