RchiOBHm_Chr3g0458491

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000345
Physical Location & Seq
Reverse (-)
7157152 .. 7157965
814 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42515

Sequence Viewer

Length: 711 bp
ATGAAAACCCCAGCAGCAGTCTGTCTTATTTTCTTGCTTTCTGTTATTCTGCTCAAGGGTTCCCACGCAGAGCCATACGAGTATCTACAATTTGTTTTGCAATACTCCAAAGGCTACTGCTACAACACCCAGATATGTTTTGCGAGGTTGCCAAAAATGTTTACCATACACGGTGTCTGGCCCGCGAACATTTCCAATCCCCTGGTGAGATGTAAACAGTCAAAGAAACTACACCAGTTCAAGCAAAACGTGATGACAACATCACTGCAAAACGATCTTGGCCAATCTTGGCCCAATGTCGAAAGAGCTAAAACCAATATAGGGTTTTGGAAAGAGGAATACGAAAAGCATGGGTCTTGTACTGCACCAGCTATCACACAGAAAGCGTACTTTGAGAGAGCTCACGAGCTGTGGAAGGAGTATGATCTCTATACAATTTTGGATAAAAAGCAGATCAAGCCGGGGCACTCATATGCACTCACTGATTTTGAAGCAGCCGTCAAGTCCAAGATCGGTACCGATACAATACCTCTCATCCTGTGCAAAGAGGACACACTAACTACTGGGAGCAGCACAACTGGATTAATTCTGAGGGAAATAGTTATCTGTTTCGATCACCAGGGAACAAATCTGGTCAATTGTACCCGGCCGACAGATTGCAAGCGGCAGAAGAGCACAACTAAAGACAACATGATATACTACGTACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

236

Amino Acids

27.0

Weight (kDa)

8.95

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 26 - 214 1.4e-36 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 515
AccB1I GGYRCC 1 cut(s) 515
AccII CGCG 1 cut(s) 185
AciI CCGC 2 cut(s) 183, 664
AcoI YGGCCR 2 cut(s) 280, 647
AfaI GTAC 5 cut(s) 361, 389, 517, 643, 705
AfiI CCNNNNNNNGG 1 cut(s) 321
AgsI TTSAA 2 cut(s) 241, 491
AjnI CCWGG 2 cut(s) 201, 618
AluBI AGCT 4 cut(s) 308, 371, 401, 409
AluI AGCT 4 cut(s) 308, 371, 401, 409
Alw21I GWGCWC 2 cut(s) 403, 677
AoxI GGCC 4 cut(s) 179, 280, 290, 647
ApeKI GCWGC 3 cut(s) 14, 494, 570
AseI ATTAAT 1 cut(s) 584
Asp718I GGTACC 1 cut(s) 515
AspS9I GGNCC 2 cut(s) 180, 291
AsuC2I CCSGG 2 cut(s) 462, 646
AsuHPI GGTGA 2 cut(s) 217, 608
BaeGI GKGCMC 1 cut(s) 468
BalI TGGCCA 1 cut(s) 282
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 403
BauI CACGAG 1 cut(s) 404
Bbv12I GWGCWC 2 cut(s) 403, 677
BbvI GCAGC 3 cut(s) 26, 506, 582
BceAI ACGGC 1 cut(s) 482
BciT130I CCWGG 2 cut(s) 203, 620
BcnI CCSGG 2 cut(s) 462, 646
BisI GCNGC 4 cut(s) 15, 495, 571, 665
BlsI GCNGC 4 cut(s) 16, 496, 572, 666
Bme1390I CCNGG 4 cut(s) 203, 462, 620, 646
BmgT120I GGNCC 2 cut(s) 180, 291
BmiI GGNNCC 2 cut(s) 61, 517
BmrFI CCNGG 4 cut(s) 203, 462, 620, 646
BmrI ACTGGG 1 cut(s) 573
BmuI ACTGGG 1 cut(s) 573
BpuEI CTTGAG 1 cut(s) 38
BpuMI CCSGG 2 cut(s) 462, 646
BsaAI YACGTR 1 cut(s) 703
BsaJI CCNNGG 3 cut(s) 201, 461, 619
Bsc4I CCNNNNNNNGG 1 cut(s) 321
Bse1I ACTGG 3 cut(s) 235, 568, 583
BseBI CCWGG 2 cut(s) 203, 620
BseDI CCNNGG 3 cut(s) 201, 461, 619
BseGI GGATG 1 cut(s) 534
BseLI CCNNNNNNNGG 1 cut(s) 321
BseMII CTCAG 1 cut(s) 581
BseNI ACTGG 3 cut(s) 235, 568, 583
BseSI GKGCMC 1 cut(s) 468
BseX3I CGGCCG 1 cut(s) 647
BseXI GCAGC 3 cut(s) 26, 506, 582
BseYI CCCAGC 1 cut(s) 10
BsgI GTGCAG 1 cut(s) 348
Bsh1236I CGCG 1 cut(s) 185
Bsh1285I CGRYCG 1 cut(s) 650
BshFI GGCC 4 cut(s) 181, 282, 292, 649
BshNI GGYRCC 1 cut(s) 515
BsiEI CGRYCG 1 cut(s) 650
BsiHKAI GWGCWC 2 cut(s) 403, 677
BsiSI CCGG 2 cut(s) 461, 646
BslI CCNNNNNNNGG 1 cut(s) 321
BsnI GGCC 4 cut(s) 181, 282, 292, 649
Bsp1286I GDGCHC 3 cut(s) 403, 468, 677
Bsp143I GATC 5 cut(s) 274, 424, 453, 510, 613
BspACI CCGC 2 cut(s) 183, 664
BspANI GGCC 4 cut(s) 181, 282, 292, 649
BspCNI CTCAG 1 cut(s) 582
BspFNI CGCG 1 cut(s) 185
BspLI GGNNCC 2 cut(s) 61, 517
BspQI GCTCTTC 1 cut(s) 665
BspT107I GGYRCC 1 cut(s) 515
BsrI ACTGG 3 cut(s) 235, 568, 583
BssECI CCNNGG 3 cut(s) 201, 461, 619
BssMI GATC 5 cut(s) 274, 424, 453, 510, 613
BssSI CACGAG 1 cut(s) 404
Bst2BI CACGAG 1 cut(s) 404
Bst2UI CCWGG 2 cut(s) 203, 620
Bst4CI ACNGT 2 cut(s) 173, 219
Bst6I CTCTTC 1 cut(s) 665
BstBAI YACGTR 1 cut(s) 703
BstC8I GCNNGC 2 cut(s) 183, 662
BstDEI CTNAG 1 cut(s) 590
BstF5I GGATG 1 cut(s) 534
BstFNI CGCG 1 cut(s) 185
BstKTI GATC 5 cut(s) 277, 427, 456, 513, 616
BstMBI GATC 5 cut(s) 274, 424, 453, 510, 613
BstMCI CGRYCG 1 cut(s) 650
BstMWI GCNNNNNNNGC 1 cut(s) 457
BstNI CCWGG 2 cut(s) 203, 620
BstSCI CCNGG 4 cut(s) 201, 460, 618, 644
BstSLI GKGCMC 1 cut(s) 468
BstSNI TACGTA 1 cut(s) 703
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 3 cut(s) 26, 506, 582
BstXI CCANNNNNNTGG 1 cut(s) 202
BstZI CGGCCG 1 cut(s) 647
BsuRI GGCC 4 cut(s) 181, 282, 292, 649
BtsCI GGATG 1 cut(s) 534
BtsI GCAGTG 1 cut(s) 263
BtsIMutI CAGTG 2 cut(s) 263, 480
Cac8I GCNNGC 2 cut(s) 183, 662
Cfr13I GGNCC 2 cut(s) 180, 291
Csp6I GTAC 5 cut(s) 360, 388, 516, 642, 704
CviAII CATG 2 cut(s) 350, 691
CviQI GTAC 5 cut(s) 360, 388, 516, 642, 704
DdeI CTNAG 1 cut(s) 590
DpnI GATC 5 cut(s) 276, 426, 455, 512, 615
DpnII GATC 5 cut(s) 274, 424, 453, 510, 613
EaeI YGGCCR 2 cut(s) 280, 647
EagI CGGCCG 1 cut(s) 647
Eam1104I CTCTTC 1 cut(s) 665
EarI CTCTTC 1 cut(s) 665
Ecl136II GAGCTC 1 cut(s) 401
EclXI CGGCCG 1 cut(s) 647
Eco105I TACGTA 1 cut(s) 703
Eco24I GRGCYC 1 cut(s) 403
Eco52I CGGCCG 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 401
EcoRII CCWGG 2 cut(s) 201, 618
EcoT38I GRGCYC 1 cut(s) 403
FaeI CATG 2 cut(s) 353, 694
FatI CATG 2 cut(s) 349, 690
FauI CCCGC 1 cut(s) 190
FauNDI CATATG 1 cut(s) 472
Fnu4HI GCNGC 4 cut(s) 15, 495, 571, 665
FokI GGATG 1 cut(s) 521
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 4 cut(s) 15, 495, 571, 665
GluI GCNGC 4 cut(s) 15, 495, 571, 665
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 4 cut(s) 181, 282, 292, 649
HapII CCGG 2 cut(s) 461, 646
Hin1II CATG 2 cut(s) 353, 694
HpaII CCGG 2 cut(s) 461, 646
HphI GGTGA 2 cut(s) 217, 608
Hpy166II GTNNAC 2 cut(s) 162, 215
Hpy188I TCNGA 1 cut(s) 591
Hpy188III TCNNGA 1 cut(s) 404
Hpy8I GTNNAC 2 cut(s) 162, 215
HpyAV CCTTC 1 cut(s) 409
HpyCH4III ACNGT 2 cut(s) 173, 219
HpyCH4IV ACGT 2 cut(s) 249, 702
HpyCH4V TGCA 6 cut(s) 100, 268, 365, 476, 543, 660
HpyF10VI GCNNNNNNNGC 1 cut(s) 457
HpyF3I CTNAG 1 cut(s) 590
HpySE526I ACGT 2 cut(s) 249, 702
Hsp92II CATG 2 cut(s) 353, 694
KpnI GGTACC 1 cut(s) 519
Kzo9I GATC 5 cut(s) 274, 424, 453, 510, 613
LguI GCTCTTC 1 cut(s) 665
LmnI GCTCC 1 cut(s) 567
Lsp1109I GCAGC 3 cut(s) 26, 506, 582
MaeII ACGT 2 cut(s) 249, 702
MalI GATC 5 cut(s) 276, 426, 455, 512, 615
MboI GATC 5 cut(s) 274, 424, 453, 510, 613
MboII GAAGA 1 cut(s) 682
MfeI CAATTG 1 cut(s) 637
MhlI GDGCHC 3 cut(s) 403, 468, 677
MlsI TGGCCA 1 cut(s) 282
MluCI AATT 4 cut(s) 89, 435, 585, 637
MluNI TGGCCA 1 cut(s) 282
MnlI CCTC 5 cut(s) 138, 328, 540, 541, 585
Mox20I TGGCCA 1 cut(s) 282
MscI TGGCCA 1 cut(s) 282
MseI TTAA 2 cut(s) 584, 709
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 1 cut(s) 282
MspI CCGG 2 cut(s) 461, 646
MspR9I CCNGG 4 cut(s) 203, 462, 620, 646
MunI CAATTG 1 cut(s) 637
MvaI CCWGG 2 cut(s) 203, 620
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 1 cut(s) 457
NciI CCSGG 2 cut(s) 462, 646
NdeI CATATG 1 cut(s) 472
NdeII GATC 5 cut(s) 274, 424, 453, 510, 613
NlaIII CATG 2 cut(s) 353, 694
NlaIV GGNNCC 2 cut(s) 61, 517
PciSI GCTCTTC 1 cut(s) 665
PkrI GCNGC 4 cut(s) 16, 496, 572, 666
Ppu21I YACGTR 1 cut(s) 703
PshBI ATTAAT 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 403
Psp6I CCWGG 2 cut(s) 201, 618
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 2 cut(s) 201, 618
PspN4I GGNNCC 2 cut(s) 61, 517
PspPI GGNCC 2 cut(s) 180, 291
RsaI GTAC 5 cut(s) 361, 389, 517, 643, 705
RsaNI GTAC 5 cut(s) 360, 388, 516, 642, 704
RseI CAYNNNNRTG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 403
SapI GCTCTTC 1 cut(s) 665
SaqAI TTAA 2 cut(s) 584, 709
SatI GCNGC 4 cut(s) 15, 495, 571, 665
Sau3AI GATC 5 cut(s) 274, 424, 453, 510, 613
Sau96I GGNCC 2 cut(s) 180, 291
ScrFI CCNGG 4 cut(s) 203, 462, 620, 646
SduI GDGCHC 3 cut(s) 403, 468, 677
SetI ASST 9 cut(s) 149, 252, 310, 373, 403, 411, 532, 705, 709
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 1 cut(s) 53
SmoI CTYRAG 1 cut(s) 53
SnaBI TACGTA 1 cut(s) 703
Sse9I AATT 4 cut(s) 89, 435, 585, 637
SsiI CCGC 2 cut(s) 183, 664
SstI GAGCTC 1 cut(s) 403
StyD4I CCNGG 4 cut(s) 201, 460, 618, 644
TaaI ACNGT 2 cut(s) 173, 219
TaiI ACGT 2 cut(s) 252, 705
TaqI TCGA 2 cut(s) 300, 612
TasI AATT 4 cut(s) 89, 435, 585, 637
TatI WGTACW 1 cut(s) 359
TauI GCSGC 1 cut(s) 667
Tru1I TTAA 2 cut(s) 584, 709
Tru9I TTAA 2 cut(s) 584, 709
TscAI CASTG 2 cut(s) 270, 487
TseI GCWGC 3 cut(s) 14, 494, 570
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 270, 487
VspI ATTAAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.