MD01G1175200.v1.1

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000345
Physical Location & Seq
Reverse (-)
27629886 .. 27630687
802 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1175200.v1.1.491

Sequence Viewer

Length: 615 bp
ATGCAACCATCTCATCTTCGGTTCACTATTCATGGTCTCTGGCCTGCGAATAAATCATCCTCTTCATCCCTTAACTGCATGGGGGATGCCTTTAATGTGAATGAGATGGATGATGCACTGAAAAAGGACCTACTCAGTTCGTGGTGGGACTGGAAAGATGGGAACCATGTCGAATTCTGGCAAAGAGAGTATGATAAGCACGGAAAGTGCTCCGACAATGTGTTCCCGAAGAGCAAGTACTTCCGGAAGACCCTAGCGACGTACCGTGATTTTGATATAGCTCAAATCCTGCAGAAGGCTAATATTGTACCCCAACCACTTCAGCCCAAGATGTCGTTGTACAAATTGTACTCCATCGATCAGATAACAAAGGCCATTGAGTCCGAAACCGGCGGACATTCCGTCAATATCAGATGCTATCAACAAGAACAGAAGAAGAGCAAGAAGAGCAAGAAGGGAAGTAATCTATTAATATTTTTGGCCCAAGTCGCCCTCTGCTATGATGAATCTGGCAATAACAGAATTGACTGTGATCCAGGCCAAACAACCAGTTGCAAGTTTAACGGCGGAGACCCGGAAACTGCACCAAGTATAGCCCACATAAAGCTATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

205

Amino Acids

23.31

Weight (kDa)

8.65

Isoelectric Point (pI)

51.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 6 - 177 4.7e-31 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 243
AciI CCGC 2 cut(s) 393, 567
AclWI GGATC 1 cut(s) 527
AcsI RAATTY 1 cut(s) 173
AcuI CTGAAG 1 cut(s) 305
AfaI GTAC 5 cut(s) 239, 263, 309, 341, 350
AfiI CCNNNNNNNGG 1 cut(s) 295
AhdI GACNNNNNGTC 1 cut(s) 401
AjnI CCWGG 1 cut(s) 535
AluBI AGCT 2 cut(s) 281, 607
AluI AGCT 2 cut(s) 281, 607
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 2 cut(s) 41, 564
AlwI GGATC 1 cut(s) 527
Aor13HI TCCGGA 1 cut(s) 243
AoxI GGCC 4 cut(s) 41, 372, 480, 538
ApoI RAATTY 1 cut(s) 173
AseI ATTAAT 1 cut(s) 470
AspS9I GGNCC 2 cut(s) 127, 481
AsuC2I CCSGG 1 cut(s) 575
AvaII GGWCC 1 cut(s) 127
BarI GAAGNNNNNNTAC 2 cut(s) 221, 253
BbsI GAAGAC 1 cut(s) 254
Bbv12I GWGCWC 1 cut(s) 212
BccI CCATC 4 cut(s) 16, 100, 152, 362
BceAI ACGGC 1 cut(s) 580
BciT130I CCWGG 1 cut(s) 537
BcnI CCSGG 1 cut(s) 575
BcoDI GTCTC 2 cut(s) 41, 564
BfaI CTAG 1 cut(s) 254
BfmI CTRYAG 1 cut(s) 290
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 537, 575
Bme18I GGWCC 1 cut(s) 127
BmeRI GACNNNNNGTC 1 cut(s) 401
BmgT120I GGNCC 2 cut(s) 127, 481
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 2 cut(s) 537, 575
BmsI GCATC 3 cut(s) 76, 103, 404
BpiI GAAGAC 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 575
Bsa29I ATCGAT 1 cut(s) 357
BsaI GGTCTC 2 cut(s) 41, 564
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 2 cut(s) 155, 549
BseAI TCCGGA 1 cut(s) 243
BseBI CCWGG 1 cut(s) 537
BseCI ATCGAT 1 cut(s) 357
BseGI GGATG 4 cut(s) 56, 65, 91, 115
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMII CTCAG 1 cut(s) 148
BseNI ACTGG 2 cut(s) 155, 549
BsgI GTGCAG 1 cut(s) 567
BshFI GGCC 4 cut(s) 43, 374, 482, 540
BshVI ATCGAT 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 212
BsiSI CCGG 3 cut(s) 244, 390, 575
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 1 cut(s) 295
BsmAI GTCTC 2 cut(s) 41, 564
BsmFI GGGAC 1 cut(s) 161
BsnI GGCC 4 cut(s) 43, 374, 482, 540
Bso31I GGTCTC 2 cut(s) 41, 564
Bsp1286I GDGCHC 1 cut(s) 212
Bsp13I TCCGGA 1 cut(s) 243
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 2 cut(s) 358, 532
BspACI CCGC 2 cut(s) 393, 567
BspANI GGCC 4 cut(s) 43, 374, 482, 540
BspCNI CTCAG 1 cut(s) 147
BspDI ATCGAT 1 cut(s) 357
BspEI TCCGGA 1 cut(s) 243
BspLI GGNNCC 1 cut(s) 164
BspMAI CTGCAG 1 cut(s) 294
BspPI GGATC 1 cut(s) 527
BspQI GCTCTTC 3 cut(s) 224, 431, 440
BspTNI GGTCTC 2 cut(s) 41, 564
BsrFI RCCGGY 1 cut(s) 389
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 2 cut(s) 155, 549
BssAI RCCGGY 1 cut(s) 389
BssMI GATC 2 cut(s) 358, 532
Bst2UI CCWGG 1 cut(s) 537
Bst4CI ACNGT 2 cut(s) 266, 530
Bst6I CTCTTC 4 cut(s) 67, 224, 431, 440
BstAUI TGTACA 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 45
BstDEI CTNAG 1 cut(s) 134
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 4 cut(s) 56, 65, 91, 115
BstKTI GATC 2 cut(s) 361, 535
BstMAI GTCTC 2 cut(s) 41, 564
BstMBI GATC 2 cut(s) 358, 532
BstMWI GCNNNNNNNGC 2 cut(s) 447, 488
BstNI CCWGG 1 cut(s) 537
BstSCI CCNGG 2 cut(s) 535, 573
BstSFI CTRYAG 1 cut(s) 290
BstV2I GAAGAC 1 cut(s) 254
Bsu15I ATCGAT 1 cut(s) 357
BsuRI GGCC 4 cut(s) 43, 374, 482, 540
BsuTUI ATCGAT 1 cut(s) 357
BtsCI GGATG 4 cut(s) 56, 65, 91, 115
BtsIMutI CAGTG 1 cut(s) 116
Cac8I GCNNGC 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 2 cut(s) 127, 481
ClaI ATCGAT 1 cut(s) 357
Csp6I GTAC 5 cut(s) 238, 262, 308, 340, 349
CviAII CATG 3 cut(s) 32, 79, 167
CviJI RGCY 9 cut(s) 43, 281, 299, 325, 374, 482, 540, 596, 607
CviKI_1 RGCY 9 cut(s) 43, 281, 299, 325, 374, 482, 540, 596, 607
CviQI GTAC 5 cut(s) 238, 262, 308, 340, 349
DdeI CTNAG 1 cut(s) 134
DpnI GATC 2 cut(s) 360, 534
DpnII GATC 2 cut(s) 358, 532
DriI GACNNNNNGTC 1 cut(s) 401
Eam1104I CTCTTC 4 cut(s) 67, 224, 431, 440
Eam1105I GACNNNNNGTC 1 cut(s) 401
EarI CTCTTC 4 cut(s) 67, 224, 431, 440
EciI GGCGGA 2 cut(s) 408, 582
Eco31I GGTCTC 2 cut(s) 41, 564
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 305
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 127
EcoRI GAATTC 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 535
FaeI CATG 3 cut(s) 35, 82, 170
FaiI YATR 9 cut(s) 33, 80, 168, 192, 278, 501, 593, 602, 610
FaqI GGGAC 1 cut(s) 161
FatI CATG 3 cut(s) 31, 78, 166
FokI GGATG 4 cut(s) 43, 52, 98, 122
FspBI CTAG 1 cut(s) 254
HaeIII GGCC 4 cut(s) 43, 374, 482, 540
HapII CCGG 3 cut(s) 244, 390, 575
Hin1II CATG 3 cut(s) 35, 82, 170
HinfI GANTC 2 cut(s) 380, 506
HpaII CCGG 3 cut(s) 244, 390, 575
Hpy166II GTNNAC 1 cut(s) 24
Hpy188I TCNGA 4 cut(s) 214, 363, 385, 413
Hpy188III TCNNGA 2 cut(s) 226, 244
Hpy8I GTNNAC 1 cut(s) 24
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 2 cut(s) 289, 448
HpyCH4III ACNGT 2 cut(s) 266, 530
HpyCH4IV ACGT 1 cut(s) 260
HpyCH4V TGCA 6 cut(s) 4, 78, 116, 292, 555, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 447, 488
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 1 cut(s) 260
Hsp92II CATG 3 cut(s) 35, 82, 170
Kpn2I TCCGGA 1 cut(s) 243
Kzo9I GATC 2 cut(s) 358, 532
LguI GCTCTTC 3 cut(s) 224, 431, 440
LmnI GCTCC 1 cut(s) 215
LweI GCATC 3 cut(s) 76, 103, 404
MaeI CTAG 1 cut(s) 254
MaeII ACGT 1 cut(s) 260
MalI GATC 2 cut(s) 360, 534
MboI GATC 2 cut(s) 358, 532
MboII GAAGA 7 cut(s) 8, 54, 241, 259, 445, 448, 457
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 3 cut(s) 173, 344, 522
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 2 cut(s) 70, 503
MroI TCCGGA 1 cut(s) 243
MseI TTAA 4 cut(s) 72, 93, 470, 561
MspI CCGG 3 cut(s) 244, 390, 575
MspR9I CCNGG 2 cut(s) 537, 575
MvaI CCWGG 1 cut(s) 537
MwoI GCNNNNNNNGC 2 cut(s) 447, 488
NciI CCSGG 1 cut(s) 575
NdeII GATC 2 cut(s) 358, 532
NlaIII CATG 3 cut(s) 35, 82, 170
NlaIV GGNNCC 1 cut(s) 164
PciSI GCTCTTC 3 cut(s) 224, 431, 440
PfeI GAWTC 1 cut(s) 506
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PpuMI RGGWCCY 1 cut(s) 127
PshBI ATTAAT 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 127
Psp6I CCWGG 1 cut(s) 535
PspGI CCWGG 1 cut(s) 535
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 2 cut(s) 127, 481
PspPPI RGGWCCY 1 cut(s) 127
PstI CTGCAG 1 cut(s) 294
RsaI GTAC 5 cut(s) 239, 263, 309, 341, 350
RsaNI GTAC 5 cut(s) 238, 262, 308, 340, 349
SapI GCTCTTC 3 cut(s) 224, 431, 440
SaqAI TTAA 4 cut(s) 72, 93, 470, 561
Sau3AI GATC 2 cut(s) 358, 532
Sau96I GGNCC 2 cut(s) 127, 481
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 537, 575
SduI GDGCHC 1 cut(s) 212
SetI ASST 4 cut(s) 132, 263, 283, 609
SfaNI GCATC 3 cut(s) 76, 103, 404
SfcI CTRYAG 1 cut(s) 290
SinI GGWCC 1 cut(s) 127
Sse9I AATT 3 cut(s) 173, 344, 522
SsiI CCGC 2 cut(s) 393, 567
SspI AATATT 2 cut(s) 304, 474
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 2 cut(s) 535, 573
TaaI ACNGT 2 cut(s) 266, 530
TaiI ACGT 1 cut(s) 263
TaqI TCGA 2 cut(s) 171, 357
TasI AATT 3 cut(s) 173, 344, 522
TatI WGTACW 3 cut(s) 237, 339, 348
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 4 cut(s) 72, 93, 470, 561
Tru9I TTAA 4 cut(s) 72, 93, 470, 561
TscAI CASTG 1 cut(s) 123
TspDTI ATGAA 3 cut(s) 20, 54, 519
TspGWI ACGGA 2 cut(s) 216, 391
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 127
VspI ATTAAT 1 cut(s) 470
XagI CCTNNNNNAGG 1 cut(s) 293
XapI RAATTY 1 cut(s) 173
XspI CTAG 1 cut(s) 254
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.