RchiOBHm_Chr2g0110251

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000345
Physical Location & Seq
Forward (+)
21731487 .. 21732077
591 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48395

Sequence Viewer

Length: 486 bp
ATGAAGAATTCAAACTTCATCATCCTTCTATCAGTATATGTACTTGTTGTACAAGTTCTCAACGTTTCAAATGCTGAACCCTACGAGTACATGCAATTTGTACTCCAGTACCCACCTGGAGTTTGCTATGACACATCAAAAGGTTATTGCATCTCACCTCTGCCAACCAAGTTCCATGTACATGGAATATGGCCTTCTAACTTCTCCGATATACATGTCACTTGTGGTCAAGCACTGAAGAATAATCCCCTTGATAACGCGCAGATGACACCTCTGCAGATGGATTTGGTAAACTCATGGCCGTCCGTGTTAACAACAAAGAGCAATATGTGGTTCTGGGAGCATGAGTACAAGAAACATGGCGCATGTACAGTAGAGTCAGGTGTACCACCTTTCACTCAAAAGTCCTACTTTGAGAAAGGACACCAGCTATGGAACCAATATGACATCCATTCAGTGCTTGATCAAAGTGGTATTAACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

161

Amino Acids

18.5

Weight (kDa)

6.12

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 160 7.4e-27 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 260
AclI AACGTT 1 cut(s) 63
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 1 cut(s) 7
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 9 cut(s) 42, 51, 89, 102, 110, 180, 350, 370, 387
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 2 cut(s) 12, 69
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 1 cut(s) 430
AluI AGCT 1 cut(s) 430
AoxI GGCC 2 cut(s) 191, 299
ApoI RAATTY 1 cut(s) 7
AspLEI GCGC 2 cut(s) 262, 365
AsuHPI GGTGA 1 cut(s) 147
BaeI ACNNNNGTAYC 2 cut(s) 92, 125
BccI CCATC 1 cut(s) 274
BceAI ACGGC 1 cut(s) 286
BciT130I CCWGG 1 cut(s) 117
BclI TGATCA 1 cut(s) 463
BfmI CTRYAG 1 cut(s) 275
Bme1390I CCNGG 1 cut(s) 117
BmiI GGNNCC 1 cut(s) 437
BmrFI CCNGG 1 cut(s) 117
BmsI GCATC 1 cut(s) 159
BpmI CTGGAG 2 cut(s) 89, 138
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bse1I ACTGG 1 cut(s) 106
BseBI CCWGG 1 cut(s) 117
BseGI GGATG 2 cut(s) 21, 447
BseNI ACTGG 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 2 cut(s) 193, 301
BsnI GGCC 2 cut(s) 193, 301
Bsp1407I TGTACA 3 cut(s) 49, 178, 368
Bsp143I GATC 1 cut(s) 463
BspANI GGCC 2 cut(s) 193, 301
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 1 cut(s) 437
BspMAI CTGCAG 1 cut(s) 279
BsrGI TGTACA 3 cut(s) 49, 178, 368
BsrI ACTGG 1 cut(s) 106
BssMI GATC 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 373
BstAUI TGTACA 3 cut(s) 49, 178, 368
BstF5I GGATG 2 cut(s) 21, 447
BstFNI CGCG 1 cut(s) 260
BstHHI GCGC 2 cut(s) 262, 365
BstKTI GATC 1 cut(s) 466
BstMBI GATC 1 cut(s) 463
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 3 cut(s) 94, 218, 369
BstSCI CCNGG 1 cut(s) 115
BstSFI CTRYAG 1 cut(s) 275
BstUI CGCG 1 cut(s) 260
BstXI CCANNNNNNTGG 1 cut(s) 182
BsuRI GGCC 2 cut(s) 193, 301
BtsCI GGATG 2 cut(s) 21, 447
BtsIMutI CAGTG 2 cut(s) 233, 462
CfoI GCGC 2 cut(s) 262, 365
Csp6I GTAC 9 cut(s) 41, 50, 88, 101, 109, 179, 349, 369, 386
CviAII CATG 8 cut(s) 91, 176, 182, 215, 297, 344, 359, 366
CviJI RGCY 3 cut(s) 193, 301, 430
CviKI_1 RGCY 3 cut(s) 193, 301, 430
CviQI GTAC 9 cut(s) 41, 50, 88, 101, 109, 179, 349, 369, 386
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
EaeI YGGCCR 1 cut(s) 299
Eco57I CTGAAG 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 115
FaeI CATG 8 cut(s) 94, 179, 185, 218, 300, 347, 362, 369
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FatI CATG 8 cut(s) 90, 175, 181, 214, 296, 343, 358, 365
FbaI TGATCA 1 cut(s) 463
FokI GGATG 2 cut(s) 8, 434
GlaI GCGC 2 cut(s) 261, 364
GsuI CTGGAG 2 cut(s) 89, 138
HaeIII GGCC 2 cut(s) 193, 301
HhaI GCGC 2 cut(s) 262, 365
Hin1II CATG 8 cut(s) 94, 179, 185, 218, 300, 347, 362, 369
Hin6I GCGC 2 cut(s) 260, 363
HinP1I GCGC 2 cut(s) 260, 363
HincII GTYRAC 1 cut(s) 312
HindII GTYRAC 1 cut(s) 312
HinfI GANTC 1 cut(s) 377
HpaI GTTAAC 1 cut(s) 312
HphI GGTGA 1 cut(s) 147
Hpy166II GTNNAC 3 cut(s) 292, 312, 386
Hpy188I TCNGA 1 cut(s) 208
Hpy8I GTNNAC 3 cut(s) 292, 312, 386
HpyAV CCTTC 2 cut(s) 35, 204
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 3 cut(s) 94, 150, 277
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 8 cut(s) 94, 179, 185, 218, 300, 347, 362, 369
HspAI GCGC 2 cut(s) 260, 363
Ksp22I TGATCA 1 cut(s) 463
KspAI GTTAAC 1 cut(s) 312
Kzo9I GATC 1 cut(s) 463
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 6 cut(s) 102, 119, 129, 322, 366, 440
LweI GCATC 1 cut(s) 159
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 217
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MboII GAAGA 2 cut(s) 16, 250
MluCI AATT 2 cut(s) 7, 95
MlyI GAGTC 1 cut(s) 386
MnlI CCTC 2 cut(s) 168, 282
MseI TTAA 3 cut(s) 311, 477, 484
MslI CAYNNNNRTG 1 cut(s) 180
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
MvnI CGCG 1 cut(s) 260
NdeII GATC 1 cut(s) 463
NlaIII CATG 8 cut(s) 94, 179, 185, 218, 300, 347, 362, 369
NlaIV GGNNCC 1 cut(s) 437
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 3 cut(s) 94, 218, 369
PciI ACATGT 1 cut(s) 214
PleI GAGTC 1 cut(s) 385
PpsI GAGTC 1 cut(s) 385
PscI ACATGT 1 cut(s) 214
Psp1406I AACGTT 1 cut(s) 63
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 437
PstI CTGCAG 1 cut(s) 279
RsaI GTAC 9 cut(s) 42, 51, 89, 102, 110, 180, 350, 370, 387
RsaNI GTAC 9 cut(s) 41, 50, 88, 101, 109, 179, 349, 369, 386
RseI CAYNNNNRTG 1 cut(s) 180
SaqAI TTAA 3 cut(s) 311, 477, 484
Sau3AI GATC 1 cut(s) 463
SchI GAGTC 1 cut(s) 386
ScrFI CCNGG 1 cut(s) 117
SetI ASST 8 cut(s) 66, 118, 145, 160, 274, 385, 394, 432
SfaNI GCATC 1 cut(s) 159
SfcI CTRYAG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 180
Sse9I AATT 2 cut(s) 7, 95
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 1 cut(s) 66
TasI AATT 2 cut(s) 7, 95
TatI WGTACW 7 cut(s) 40, 49, 87, 100, 178, 348, 368
Tru1I TTAA 3 cut(s) 311, 477, 484
Tru9I TTAA 3 cut(s) 311, 477, 484
TscAI CASTG 2 cut(s) 240, 462
TseFI GTSAC 1 cut(s) 217
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 7, 17
TspGWI ACGGA 1 cut(s) 295
TspRI CASTG 2 cut(s) 240, 462
XapI RAATTY 1 cut(s) 7
XceI RCATGY 3 cut(s) 94, 218, 369
XcmI CCANNNNNNNNNTGG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.