Rh2BG313300

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000345
Physical Location & Seq
Reverse (-)
36862613 .. 36863693
1081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG313300.1

Sequence Viewer

Length: 699 bp
ATGAAAACAGCAGCAGTCTGTCTCCTTTTCTTGCTCTCTGTTATACTGTTCAAGGGTTCCCATGCAAAGCCATACGAATACCTACAGTTTGTCTTGCAATACCCTAGAGGTTACTGTGTTAACGACAAGAAATGCATTCCACCGGCGAGCTTGCCAGCCAAATTCACGGTTCATGGAATTTGGCCCACGAATATTTCCGAGCCGATTATCAGCTGTGACAAGGCACAGAAACTACACTCGTTCAACGGAAACCTGATAACTCCGTCGTTGAAAACCGATCTTCTCCAATCTTGGCCCAGCGTCGTGACAAATAAAGATGATATGTCGTTTTGGGAACACGAATATAATAAGCATGGGTCCTGTACTGCACCAGCTATCACTCAGACAGCGTATTTTGAGAGAGCTCACAAGTTCTGGAAGGAGTATGATCTCTATTCCATTTTGGAACAAAAAAATATCAAGCCGGGGCAATCTCGATCGTATTCACTGGCTGATTTTGAAGCAGCCGTCAAGTCCAAGATCGGTACCGACACAAAACCTCTCATCCTTTGTAAGCAGGACAAACTAACTCGTGGGAGCACAACAACCAACTTCATTTTGAGGGAAATTGTGATCTGTTTCGATCACCAGGGGACAAATCTGGTCAATTGTACCCGATCAACATATTGTGACCAGAAGCAGAAGATATACTACCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

232

Amino Acids

26.49

Weight (kDa)

8.95

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 25 - 217 2e-42 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 524
AccB1I GGYRCC 1 cut(s) 524
AcsI RAATTY 2 cut(s) 161, 177
AfaI GTAC 3 cut(s) 364, 526, 652
AgsI TTSAA 4 cut(s) 52, 244, 271, 500
AjnI CCWGG 1 cut(s) 627
AluBI AGCT 4 cut(s) 150, 213, 374, 404
AluI AGCT 4 cut(s) 150, 213, 374, 404
Alw21I GWGCWC 2 cut(s) 406, 581
Alw26I GTCTC 1 cut(s) 26
AoxI GGCC 2 cut(s) 182, 293
ApeKI GCWGC 2 cut(s) 11, 503
ApoI RAATTY 2 cut(s) 161, 177
Asp718I GGTACC 1 cut(s) 524
AspS9I GGNCC 3 cut(s) 183, 294, 357
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 1 cut(s) 617
AvaII GGWCC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 524
BanII GRGCYC 1 cut(s) 406
BarI GAAGNNNNNNTAC 1 cut(s) 674
BauI CACGAG 1 cut(s) 570
Bbv12I GWGCWC 2 cut(s) 406, 581
BbvI GCAGC 2 cut(s) 23, 515
BceAI ACGGC 1 cut(s) 491
BciT130I CCWGG 1 cut(s) 629
BcnI CCSGG 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 83
BisI GCNGC 2 cut(s) 12, 504
BlsI GCNGC 2 cut(s) 13, 505
Bme1390I CCNGG 2 cut(s) 465, 629
Bme18I GGWCC 1 cut(s) 357
BmgT120I GGNCC 3 cut(s) 183, 294, 357
BmiI GGNNCC 3 cut(s) 58, 358, 526
BmrFI CCNGG 2 cut(s) 465, 629
BpuMI CCSGG 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 464, 628
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 492
BseBI CCWGG 1 cut(s) 629
BseDI CCNNGG 2 cut(s) 464, 628
BseGI GGATG 1 cut(s) 543
BseMII CTCAG 1 cut(s) 395
BseNI ACTGG 1 cut(s) 492
BseXI GCAGC 2 cut(s) 23, 515
BseYI CCCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 351
Bsh1285I CGRYCG 1 cut(s) 479
BshFI GGCC 2 cut(s) 184, 295
BshNI GGYRCC 1 cut(s) 524
BsiEI CGRYCG 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 406, 581
BsiSI CCGG 2 cut(s) 143, 464
BslFI GGGAC 1 cut(s) 646
BsmAI GTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 646
BsmI GAATGC 1 cut(s) 135
BsnI GGCC 2 cut(s) 184, 295
Bsp1286I GDGCHC 2 cut(s) 406, 581
Bsp143I GATC 7 cut(s) 277, 427, 476, 519, 612, 622, 656
BspANI GGCC 2 cut(s) 184, 295
BspCNI CTCAG 1 cut(s) 394
BspLI GGNNCC 3 cut(s) 58, 358, 526
BspT107I GGYRCC 1 cut(s) 524
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 1 cut(s) 492
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 2 cut(s) 464, 628
BssMI GATC 7 cut(s) 277, 427, 476, 519, 612, 622, 656
BssSI CACGAG 1 cut(s) 570
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 629
Bst4CI ACNGT 4 cut(s) 48, 87, 116, 169
BstC8I GCNNGC 3 cut(s) 148, 152, 156
BstDEI CTNAG 2 cut(s) 381, 696
BstF5I GGATG 1 cut(s) 543
BstKTI GATC 7 cut(s) 280, 430, 479, 522, 615, 625, 659
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 7 cut(s) 277, 427, 476, 519, 612, 622, 656
BstMCI CGRYCG 1 cut(s) 479
BstNI CCWGG 1 cut(s) 629
BstSCI CCNGG 2 cut(s) 463, 627
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 23, 515
BsuRI GGCC 2 cut(s) 184, 295
BtsCI GGATG 1 cut(s) 543
BtsIMutI CAGTG 1 cut(s) 485
Cac8I GCNNGC 3 cut(s) 148, 152, 156
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 3 cut(s) 183, 294, 357
CseI GACGC 1 cut(s) 289
Csp6I GTAC 3 cut(s) 363, 525, 651
CviAII CATG 3 cut(s) 62, 173, 353
CviQI GTAC 3 cut(s) 363, 525, 651
DdeI CTNAG 2 cut(s) 381, 696
DpnI GATC 7 cut(s) 279, 429, 478, 521, 614, 624, 658
DpnII GATC 7 cut(s) 277, 427, 476, 519, 612, 622, 656
Ecl136II GAGCTC 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 406
Eco47I GGWCC 1 cut(s) 357
Eco53kI GAGCTC 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 627
EcoT22I ATGCAT 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 406
FaeI CATG 3 cut(s) 65, 176, 356
FaqI GGGAC 1 cut(s) 646
FatI CATG 3 cut(s) 61, 172, 352
Fnu4HI GCNGC 2 cut(s) 12, 504
FokI GGATG 1 cut(s) 530
FriOI GRGCYC 1 cut(s) 406
Fsp4HI GCNGC 2 cut(s) 12, 504
FspBI CTAG 1 cut(s) 105
GluI GCNGC 2 cut(s) 12, 504
GsaI CCCAGC 1 cut(s) 300
HaeIII GGCC 2 cut(s) 184, 295
HapII CCGG 2 cut(s) 143, 464
HgaI GACGC 1 cut(s) 289
Hin1II CATG 3 cut(s) 65, 176, 356
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HpaI GTTAAC 1 cut(s) 121
HpaII CCGG 2 cut(s) 143, 464
HphI GGTGA 1 cut(s) 617
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 2 cut(s) 199, 384
Hpy188III TCNNGA 3 cut(s) 304, 415, 474
Hpy8I GTNNAC 1 cut(s) 121
Hpy99I CGWCG 2 cut(s) 268, 305
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 4 cut(s) 48, 87, 116, 169
HpyCH4V TGCA 4 cut(s) 65, 97, 135, 368
HpyF3I CTNAG 2 cut(s) 381, 696
Hsp92II CATG 3 cut(s) 65, 176, 356
KpnI GGTACC 1 cut(s) 528
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 7 cut(s) 277, 427, 476, 519, 612, 622, 656
LmnI GCTCC 1 cut(s) 576
Lsp1109I GCAGC 2 cut(s) 23, 515
MaeI CTAG 1 cut(s) 105
MaeIII GTNAC 4 cut(s) 110, 215, 304, 668
MalI GATC 7 cut(s) 279, 429, 478, 521, 614, 624, 658
MboI GATC 7 cut(s) 277, 427, 476, 519, 612, 622, 656
MboII GAAGA 2 cut(s) 272, 694
MfeI CAATTG 1 cut(s) 646
MhlI GDGCHC 2 cut(s) 406, 581
MluCI AATT 4 cut(s) 161, 177, 606, 646
MnlI CCTC 3 cut(s) 101, 549, 594
Mph1103I ATGCAT 1 cut(s) 137
MseI TTAA 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 213
MspI CCGG 2 cut(s) 143, 464
MspR9I CCNGG 2 cut(s) 465, 629
MunI CAATTG 1 cut(s) 646
Mva1269I GAATGC 1 cut(s) 135
MvaI CCWGG 1 cut(s) 629
NciI CCSGG 1 cut(s) 465
NdeII GATC 7 cut(s) 277, 427, 476, 519, 612, 622, 656
NlaIII CATG 3 cut(s) 65, 176, 356
NlaIV GGNNCC 3 cut(s) 58, 358, 526
NmuCI GTSAC 3 cut(s) 215, 304, 668
NsiI ATGCAT 1 cut(s) 137
PctI GAATGC 1 cut(s) 135
PkrI GCNGC 2 cut(s) 13, 505
Ple19I CGATCG 1 cut(s) 479
PpuMI RGGWCCY 1 cut(s) 357
Psp124BI GAGCTC 1 cut(s) 406
Psp5II RGGWCCY 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 627
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 1 cut(s) 627
PspN4I GGNNCC 3 cut(s) 58, 358, 526
PspPI GGNCC 3 cut(s) 183, 294, 357
PspPPI RGGWCCY 1 cut(s) 357
PvuI CGATCG 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 213
RsaI GTAC 3 cut(s) 364, 526, 652
RsaNI GTAC 3 cut(s) 363, 525, 651
SacI GAGCTC 1 cut(s) 406
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 2 cut(s) 12, 504
Sau3AI GATC 7 cut(s) 277, 427, 476, 519, 612, 622, 656
Sau96I GGNCC 3 cut(s) 183, 294, 357
ScrFI CCNGG 2 cut(s) 465, 629
SduI GDGCHC 2 cut(s) 406, 581
SetI ASST 8 cut(s) 84, 112, 152, 215, 255, 376, 406, 541
SfcI CTRYAG 1 cut(s) 83
SgrAI CRCCGGYG 1 cut(s) 142
SinI GGWCC 1 cut(s) 357
Sse9I AATT 4 cut(s) 161, 177, 606, 646
SspI AATATT 1 cut(s) 193
SspMI CTAG 1 cut(s) 105
SstI GAGCTC 1 cut(s) 406
StyD4I CCNGG 2 cut(s) 463, 627
TaaI ACNGT 4 cut(s) 48, 87, 116, 169
TaqI TCGA 2 cut(s) 475, 621
TasI AATT 4 cut(s) 161, 177, 606, 646
TatI WGTACW 1 cut(s) 362
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 1 cut(s) 492
TseFI GTSAC 3 cut(s) 215, 304, 668
TseI GCWGC 2 cut(s) 11, 503
Tsp45I GTSAC 3 cut(s) 215, 304, 668
TspDTI ATGAA 3 cut(s) 17, 161, 583
TspGWI ACGGA 2 cut(s) 252, 261
TspRI CASTG 1 cut(s) 492
VpaK11BI GGWCC 1 cut(s) 357
XapI RAATTY 2 cut(s) 161, 177
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.