RLG00000025231

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000345
Physical Location & Seq
Reverse (-)
43209565 .. 43210186
622 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025231

Sequence Viewer

Length: 495 bp
ATGGCATTCCTAGCAGTTAGGACACTAATAATTGTTCTTATGCTTATTTCTACATTTGCTAAAGCCGCAAAAAAATATGACTATCTACAACCAGTGCAACAATGGCCTAAAACGTTCTGCCATAATAACCAAGCTTGCATTCAAGGTGCAGCCCTCCCGGAGCTGTTCTCGATACATGGTATGTGGCCATCTAATTTCTCCAGCCAGAACGACGCTTGTGTTGGAACTCGGTTCAGCATGAGAGAGATGCGTCGACACAATCATCTGGAAACTCAATTGCTGTCATCATCATGGCCAAGCTACACTGGTCTGTCCAACCTGCACTTTTGGGAGTACGAGTATAACAAGCACGGCACTTGTTCAGAGAATAACCTCAAACAGACGGACTATTTCACCAGAGCCGACGCTTTGTGGAGGCGCTACAATGTTACCAATTTGCTTGTAACATCGCGCCACCAAATCTCGCCGGGATCCTCCTACCGTACGTTACGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

165

Amino Acids

19.1

Weight (kDa)

9.44

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 26 - 160 1.6e-26 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 327
AccI GTMKAC 1 cut(s) 253
AccII CGCG 1 cut(s) 451
AciI CCGC 1 cut(s) 66
AclI AACGTT 1 cut(s) 113
AclWI GGATC 2 cut(s) 465, 478
AcoI YGGCCR 2 cut(s) 185, 293
AfaI GTAC 2 cut(s) 335, 484
AgsI TTSAA 1 cut(s) 143
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
AluBI AGCT 3 cut(s) 134, 163, 300
AluI AGCT 3 cut(s) 134, 163, 300
AlwI GGATC 2 cut(s) 465, 478
AoxI GGCC 3 cut(s) 104, 185, 293
ApeKI GCWGC 1 cut(s) 149
AspLEI GCGC 2 cut(s) 420, 453
AsuC2I CCSGG 2 cut(s) 158, 468
AsuHPI GGTGA 1 cut(s) 385
BalI TGGCCA 2 cut(s) 187, 295
BamHI GGATCC 1 cut(s) 470
BbvI GCAGC 1 cut(s) 161
BccI CCATC 1 cut(s) 196
BceAI ACGGC 1 cut(s) 367
BcnI CCSGG 2 cut(s) 158, 468
BfaI CTAG 1 cut(s) 11
BfoI RGCGCY 1 cut(s) 421
BfuAI ACCTGC 1 cut(s) 327
BisI GCNGC 2 cut(s) 66, 150
BlsI GCNGC 2 cut(s) 67, 151
Bme1390I CCNGG 2 cut(s) 158, 468
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 2 cut(s) 158, 468
BmsI GCATC 1 cut(s) 237
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BpmI CTGGAG 1 cut(s) 184
BpuMI CCSGG 2 cut(s) 158, 468
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bse1I ACTGG 2 cut(s) 92, 310
BseNI ACTGG 2 cut(s) 92, 310
BseXI GCAGC 1 cut(s) 161
BsgI GTGCAG 2 cut(s) 168, 305
Bsh1236I CGCG 1 cut(s) 451
BshFI GGCC 3 cut(s) 106, 187, 295
BsiSI CCGG 2 cut(s) 158, 467
BsiWI CGTACG 1 cut(s) 482
BsmI GAATGC 2 cut(s) 5, 138
BsnI GGCC 3 cut(s) 106, 187, 295
Bsp143I GATC 1 cut(s) 470
BspACI CCGC 1 cut(s) 66
BspANI GGCC 3 cut(s) 106, 187, 295
BspFNI CGCG 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 472
BspMI ACCTGC 1 cut(s) 327
BspPI GGATC 2 cut(s) 465, 478
BsrI ACTGG 2 cut(s) 92, 310
BssMI GATC 1 cut(s) 470
Bst4CI ACNGT 1 cut(s) 482
BstC8I GCNNGC 1 cut(s) 136
BstFNI CGCG 1 cut(s) 451
BstH2I RGCGCY 1 cut(s) 421
BstHHI GCGC 2 cut(s) 420, 453
BstKTI GATC 1 cut(s) 473
BstMBI GATC 1 cut(s) 470
BstMWI GCNNNNNNNGC 3 cut(s) 11, 65, 103
BstSCI CCNGG 2 cut(s) 156, 466
BstUI CGCG 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 161
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BsuRI GGCC 3 cut(s) 106, 187, 295
BtgZI GCGATG 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 99, 303
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 136
CfoI GCGC 2 cut(s) 420, 453
CseI GACGC 3 cut(s) 221, 239, 413
Csp6I GTAC 2 cut(s) 334, 483
CviAII CATG 3 cut(s) 176, 238, 291
CviQI GTAC 2 cut(s) 334, 483
DpnI GATC 1 cut(s) 472
DpnII GATC 1 cut(s) 470
EaeI YGGCCR 2 cut(s) 185, 293
FaeI CATG 3 cut(s) 179, 241, 294
FaiI YATR 8 cut(s) 41, 78, 123, 177, 182, 239, 292, 342
FatI CATG 3 cut(s) 175, 237, 290
FblI GTMKAC 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 66, 150
Fsp4HI GCNGC 2 cut(s) 66, 150
FspBI CTAG 1 cut(s) 11
GlaI GCGC 2 cut(s) 419, 452
GluI GCNGC 2 cut(s) 66, 150
GsuI CTGGAG 1 cut(s) 184
HaeII RGCGCY 1 cut(s) 421
HaeIII GGCC 3 cut(s) 106, 187, 295
HapII CCGG 2 cut(s) 158, 467
HgaI GACGC 3 cut(s) 221, 239, 413
HhaI GCGC 2 cut(s) 420, 453
Hin1II CATG 3 cut(s) 179, 241, 294
Hin6I GCGC 2 cut(s) 418, 451
HinP1I GCGC 2 cut(s) 418, 451
HincII GTYRAC 1 cut(s) 254
HindII GTYRAC 1 cut(s) 254
HindIII AAGCTT 1 cut(s) 132
HpaII CCGG 2 cut(s) 158, 467
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 1 cut(s) 254
Hpy188I TCNGA 1 cut(s) 364
Hpy188III TCNNGA 2 cut(s) 169, 266
Hpy8I GTNNAC 1 cut(s) 254
Hpy99I CGWCG 3 cut(s) 215, 255, 407
HpyCH4III ACNGT 1 cut(s) 482
HpyCH4IV ACGT 2 cut(s) 113, 485
HpyCH4V TGCA 4 cut(s) 97, 138, 149, 322
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 65, 103
HpySE526I ACGT 2 cut(s) 113, 485
Hsp92II CATG 3 cut(s) 179, 241, 294
HspAI GCGC 2 cut(s) 418, 451
Kzo9I GATC 1 cut(s) 470
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 9 cut(s) 105, 171, 214, 218, 251, 291, 332, 409, 480
Lsp1109I GCAGC 1 cut(s) 161
LweI GCATC 1 cut(s) 237
MaeI CTAG 1 cut(s) 11
MaeII ACGT 2 cut(s) 113, 485
MaeIII GTNAC 3 cut(s) 427, 442, 486
MalI GATC 1 cut(s) 472
MboI GATC 1 cut(s) 470
MfeI CAATTG 1 cut(s) 275
MflI RGATCY 1 cut(s) 470
MlsI TGGCCA 2 cut(s) 187, 295
MluCI AATT 4 cut(s) 30, 193, 275, 433
MluNI TGGCCA 2 cut(s) 187, 295
MmeI TCCRAC 2 cut(s) 202, 339
MnlI CCTC 4 cut(s) 164, 383, 408, 484
Mox20I TGGCCA 2 cut(s) 187, 295
MscI TGGCCA 2 cut(s) 187, 295
MslI CAYNNNNRTG 1 cut(s) 289
Msp20I TGGCCA 2 cut(s) 187, 295
MspI CCGG 2 cut(s) 158, 467
MspR9I CCNGG 2 cut(s) 158, 468
MunI CAATTG 1 cut(s) 275
Mva1269I GAATGC 2 cut(s) 5, 138
MvnI CGCG 1 cut(s) 451
MwoI GCNNNNNNNGC 3 cut(s) 11, 65, 103
NciI CCSGG 2 cut(s) 158, 468
NdeII GATC 1 cut(s) 470
NlaIII CATG 3 cut(s) 179, 241, 294
NlaIV GGNNCC 1 cut(s) 472
PctI GAATGC 2 cut(s) 5, 138
Pfl23II CGTACG 1 cut(s) 482
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 2 cut(s) 67, 151
Psp1406I AACGTT 1 cut(s) 113
PspLI CGTACG 1 cut(s) 482
PspN4I GGNNCC 1 cut(s) 472
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 2 cut(s) 335, 484
RsaNI GTAC 2 cut(s) 334, 483
RseI CAYNNNNRTG 1 cut(s) 289
SalI GTCGAC 1 cut(s) 252
SatI GCNGC 2 cut(s) 66, 150
Sau3AI GATC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 158, 468
SetI ASST 8 cut(s) 116, 136, 148, 165, 302, 321, 375, 488
SfaNI GCATC 1 cut(s) 237
SmiMI CAYNNNNRTG 1 cut(s) 289
Sse9I AATT 4 cut(s) 30, 193, 275, 433
SsiI CCGC 1 cut(s) 66
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 2 cut(s) 156, 466
TaaI ACNGT 1 cut(s) 482
TaiI ACGT 2 cut(s) 116, 488
TaqI TCGA 2 cut(s) 170, 253
TasI AATT 4 cut(s) 30, 193, 275, 433
TauI GCSGC 1 cut(s) 68
TscAI CASTG 2 cut(s) 99, 310
TseI GCWGC 1 cut(s) 149
TspGWI ACGGA 1 cut(s) 398
TspRI CASTG 2 cut(s) 99, 310
XcmI CCANNNNNNNNNTGG 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 253
XspI CTAG 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.