Rmu_sc0004094.1_g000043

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004094.1
OG0000345
Physical Location & Seq
Forward (+)
207707 .. 208169
463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004094.1_g000043.1.cds

Sequence Viewer

Length: 324 bp
atgcattccaccggcgagcttgccggccaaattcacggttcatggaatttggcccacgaatatttccaagcaggaggtgatcgaataacaccgtcgctggaaaccgatcttctccaatcttggcccagcttccggagagataaagataatatgactttttgggaacacgaatataataagcatgggtcctgtactgcaccagctatcactcagacagcgtattttgagagagctcacaagttctggaatgagtatgatctctattctgttttggaacaaaaaaaaaatcaagccggggcagtctcgatggtattcactggctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

107

Amino Acids

12.32

Weight (kDa)

5.8

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 132
AcoI YGGCCR 1 cut(s) 25
AcsI RAATTY 2 cut(s) 30, 46
AfaI GTAC 1 cut(s) 193
AfiI CCNNNNNNNGG 1 cut(s) 132
AluBI AGCT 4 cut(s) 19, 129, 203, 233
AluI AGCT 4 cut(s) 19, 129, 203, 233
Alw21I GWGCWC 1 cut(s) 235
Alw26I GTCTC 1 cut(s) 307
Aor13HI TCCGGA 1 cut(s) 132
AoxI GGCC 3 cut(s) 25, 51, 122
ApoI RAATTY 2 cut(s) 30, 46
AspS9I GGNCC 3 cut(s) 52, 123, 186
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 186
BanII GRGCYC 1 cut(s) 235
Bbv12I GWGCWC 1 cut(s) 235
BccI CCATC 1 cut(s) 301
BcnI CCSGG 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 307
Bme1390I CCNGG 1 cut(s) 295
Bme18I GGWCC 1 cut(s) 186
BmgT120I GGNCC 3 cut(s) 52, 123, 186
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 295
BpuMI CCSGG 1 cut(s) 295
BsaJI CCNNGG 1 cut(s) 294
BsaWI WCCGGW 1 cut(s) 132
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse118I RCCGGY 2 cut(s) 11, 23
Bse1I ACTGG 1 cut(s) 322
BseAI TCCGGA 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 322
BseYI CCCAGC 1 cut(s) 125
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 3 cut(s) 27, 53, 124
BsiHKAI GWGCWC 1 cut(s) 235
BsiSI CCGG 4 cut(s) 12, 24, 133, 294
BslI CCNNNNNNNGG 1 cut(s) 132
BsmAI GTCTC 1 cut(s) 307
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 3 cut(s) 27, 53, 124
Bsp1286I GDGCHC 1 cut(s) 235
Bsp13I TCCGGA 1 cut(s) 132
Bsp143I GATC 3 cut(s) 79, 106, 256
BspANI GGCC 3 cut(s) 27, 53, 124
BspCNI CTCAG 1 cut(s) 223
BspEI TCCGGA 1 cut(s) 132
BspLI GGNNCC 1 cut(s) 187
BsrFI RCCGGY 2 cut(s) 11, 23
BsrI ACTGG 1 cut(s) 322
BssAI RCCGGY 2 cut(s) 11, 23
BssECI CCNNGG 1 cut(s) 294
BssMI GATC 3 cut(s) 79, 106, 256
Bst4CI ACNGT 2 cut(s) 38, 93
BstC8I GCNNGC 3 cut(s) 17, 21, 25
BstDEI CTNAG 1 cut(s) 210
BstKTI GATC 3 cut(s) 82, 109, 259
BstMAI GTCTC 1 cut(s) 307
BstMBI GATC 3 cut(s) 79, 106, 256
BstSCI CCNGG 1 cut(s) 293
BsuRI GGCC 3 cut(s) 27, 53, 124
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 3 cut(s) 17, 21, 25
Cfr10I RCCGGY 2 cut(s) 11, 23
Cfr13I GGNCC 3 cut(s) 52, 123, 186
Csp6I GTAC 1 cut(s) 192
CviAII CATG 2 cut(s) 42, 182
CviJI RGCY 9 cut(s) 19, 27, 53, 124, 129, 203, 233, 293, 321
CviKI_1 RGCY 9 cut(s) 19, 27, 53, 124, 129, 203, 233, 293, 321
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 1 cut(s) 210
DpnI GATC 3 cut(s) 81, 108, 258
DpnII GATC 3 cut(s) 79, 106, 256
EaeI YGGCCR 1 cut(s) 25
Ecl136II GAGCTC 1 cut(s) 233
Eco24I GRGCYC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 233
EcoO109I RGGNCCY 1 cut(s) 186
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 2 cut(s) 45, 185
FaiI YATR 5 cut(s) 43, 152, 174, 183, 255
FatI CATG 2 cut(s) 41, 181
FriOI GRGCYC 1 cut(s) 235
GsaI CCCAGC 1 cut(s) 129
HaeIII GGCC 3 cut(s) 27, 53, 124
HapII CCGG 4 cut(s) 12, 24, 133, 294
Hin1II CATG 2 cut(s) 45, 185
HpaII CCGG 4 cut(s) 12, 24, 133, 294
HphI GGTGA 1 cut(s) 89
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 3 cut(s) 133, 244, 304
Hpy99I CGWCG 1 cut(s) 97
HpyCH4III ACNGT 2 cut(s) 38, 93
HpyCH4V TGCA 2 cut(s) 4, 197
HpyF3I CTNAG 1 cut(s) 210
Hsp92II CATG 2 cut(s) 45, 185
Kpn2I TCCGGA 1 cut(s) 132
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Kzo9I GATC 3 cut(s) 79, 106, 256
MalI GATC 3 cut(s) 81, 108, 258
MboI GATC 3 cut(s) 79, 106, 256
MboII GAAGA 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 2 cut(s) 30, 46
MnlI CCTC 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 132
MroNI GCCGGC 1 cut(s) 23
MspI CCGG 4 cut(s) 12, 24, 133, 294
MspR9I CCNGG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 4
NaeI GCCGGC 1 cut(s) 25
NciI CCSGG 1 cut(s) 295
NdeII GATC 3 cut(s) 79, 106, 256
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 2 cut(s) 45, 185
NlaIV GGNNCC 1 cut(s) 187
NsiI ATGCAT 1 cut(s) 6
PctI GAATGC 1 cut(s) 4
PdiI GCCGGC 1 cut(s) 25
PpuMI RGGWCCY 1 cut(s) 186
Psp124BI GAGCTC 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 186
PspFI CCCAGC 1 cut(s) 125
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 3 cut(s) 52, 123, 186
PspPPI RGGWCCY 1 cut(s) 186
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
SacI GAGCTC 1 cut(s) 235
Sau3AI GATC 3 cut(s) 79, 106, 256
Sau96I GGNCC 3 cut(s) 52, 123, 186
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 1 cut(s) 235
SetI ASST 5 cut(s) 21, 79, 131, 205, 235
SgrAI CRCCGGYG 1 cut(s) 11
SinI GGWCC 1 cut(s) 186
Sse9I AATT 2 cut(s) 30, 46
SspI AATATT 1 cut(s) 62
SstI GAGCTC 1 cut(s) 235
StyD4I CCNGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 38, 93
TaqI TCGA 2 cut(s) 82, 305
TasI AATT 2 cut(s) 30, 46
TatI WGTACW 1 cut(s) 191
TscAI CASTG 1 cut(s) 322
TspDTI ATGAA 1 cut(s) 30
TspRI CASTG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 186
XapI RAATTY 2 cut(s) 30, 46
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.