MD15G1167800.v1.1

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000345
Physical Location & Seq
Reverse (-)
12842773 .. 12844293
1521 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1167800.v1.1.491

Sequence Viewer

Length: 753 bp
ATGGAGTCGAAGTTAGTGCAAGTCATATTTTTCTTCTTGTTTGTCACAGTTTCATCCGCTTACACACCATTCCAATACTTTATGCATGTTCAGTTCTGGCCAGCTGCTGAATGCCAAGCTACAGGGGGGGTGAATAAATGCCACATCATGCAACCATCTCATCTTCAGTTCACTATTCATGGTCTCTGGCCTGCGAATAAATCATCCTCTTCATCCCTTGGCTGCAAAGGGGATGCCTTTAATGTGAATGAGATGAATGATACACTGAAAAAGGAGCTACTCAGTTCGTGGTGGGATTGGAGAAAAGGGGAGCATGTCGAATTCTGGCAACGAGAGTATGATAAGCACGGAAAGTGCTCCGACAATGTGTTCCTGAAGACCGAGTACTTCCGGAAGACCCTAGCGATGTACCATGATTTTGATATAGCTCAAATCCTGCAGAAGGCTAATATTGTACCCCAACTACTTCAGCCCAAGACGTCGTTGTACAAATTGTACTCCATCGATCAGATAACAAAGGCCATTGAGTCCAAAACCGGCGGACATTCCGTCAATATCAGATGCTATCAACAAAACCAGAAGAAGAGCAACAATGGAAGTAAACCATTAATATATTTGGCCCAAGTCGCCCTCTGCTATAATAGATCTGGCAATAGCAGAACTAACTGTGATCCAGGCGAAACAACCAGCTGCAACTTTAATGAATCAGAACCGAAAACTGTACCAAGAATAGCCCACATAAAGCTCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

251

Amino Acids

28.8

Weight (kDa)

8.92

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 24 - 223 3.4e-36 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 482
AccIII TCCGGA 1 cut(s) 390
AciI CCGC 2 cut(s) 57, 540
AclWI GGATC 1 cut(s) 665
AcoI YGGCCR 1 cut(s) 98
AcsI RAATTY 1 cut(s) 320
AcuI CTGAAG 3 cut(s) 149, 395, 452
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 6 cut(s) 386, 410, 456, 488, 497, 723
AfiI CCNNNNNNNGG 1 cut(s) 442
AhdI GACNNNNNGTC 1 cut(s) 548
AjnI CCWGG 1 cut(s) 673
AluBI AGCT 6 cut(s) 104, 119, 277, 428, 690, 745
AluI AGCT 6 cut(s) 104, 119, 277, 428, 690, 745
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 1 cut(s) 188
AlwI GGATC 1 cut(s) 665
AlwNI CAGNNNCTG 1 cut(s) 107
Aor13HI TCCGGA 1 cut(s) 390
AoxI GGCC 4 cut(s) 98, 188, 519, 618
ApeKI GCWGC 3 cut(s) 104, 222, 690
ApoI RAATTY 1 cut(s) 320
AseI ATTAAT 1 cut(s) 608
AspS9I GGNCC 1 cut(s) 619
AsuHPI GGTGA 1 cut(s) 142
BalI TGGCCA 1 cut(s) 100
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
BbsI GAAGAC 2 cut(s) 383, 401
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 3 cut(s) 91, 209, 677
BccI CCATC 2 cut(s) 163, 509
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 675
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 1 cut(s) 401
BfmI CTRYAG 2 cut(s) 120, 437
BglII AGATCT 1 cut(s) 644
BisI GCNGC 3 cut(s) 105, 223, 691
BlsI GCNGC 3 cut(s) 106, 224, 692
BmcAI AGTACT 1 cut(s) 386
Bme1390I CCNGG 1 cut(s) 675
BmeRI GACNNNNNGTC 1 cut(s) 548
BmgT120I GGNCC 1 cut(s) 619
BmrFI CCNGG 1 cut(s) 675
BmsI GCATC 2 cut(s) 223, 551
BpiI GAAGAC 2 cut(s) 383, 401
Bsa29I ATCGAT 1 cut(s) 504
BsaHI GRCGYC 1 cut(s) 479
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 217
BsaWI WCCGGW 1 cut(s) 390
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse118I RCCGGY 1 cut(s) 536
BseAI TCCGGA 1 cut(s) 390
BseBI CCWGG 1 cut(s) 675
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 4 cut(s) 53, 203, 212, 238
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMII CTCAG 1 cut(s) 295
BseXI GCAGC 3 cut(s) 91, 209, 677
BshFI GGCC 4 cut(s) 100, 190, 521, 620
BshVI ATCGAT 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 359
BsiSI CCGG 2 cut(s) 391, 537
BslI CCNNNNNNNGG 1 cut(s) 442
BsmAI GTCTC 1 cut(s) 188
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 4 cut(s) 100, 190, 521, 620
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 359
Bsp13I TCCGGA 1 cut(s) 390
Bsp1407I TGTACA 1 cut(s) 486
Bsp143I GATC 3 cut(s) 505, 644, 670
BspACI CCGC 2 cut(s) 57, 540
BspANI GGCC 4 cut(s) 100, 190, 521, 620
BspCNI CTCAG 1 cut(s) 294
BspDI ATCGAT 1 cut(s) 504
BspEI TCCGGA 1 cut(s) 390
BspMAI CTGCAG 1 cut(s) 441
BspPI GGATC 1 cut(s) 665
BspQI GCTCTTC 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 188
BsrFI RCCGGY 1 cut(s) 536
BsrGI TGTACA 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 536
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 3 cut(s) 505, 644, 670
BssNI GRCGYC 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 3 cut(s) 49, 668, 721
Bst6I CTCTTC 2 cut(s) 214, 578
BstACI GRCGYC 1 cut(s) 479
BstAUI TGTACA 1 cut(s) 486
BstC8I GCNNGC 2 cut(s) 102, 192
BstDEI CTNAG 1 cut(s) 281
BstENI CCTNNNNNAGG 1 cut(s) 440
BstF5I GGATG 4 cut(s) 53, 203, 212, 238
BstKTI GATC 3 cut(s) 508, 647, 673
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 3 cut(s) 505, 644, 670
BstMWI GCNNNNNNNGC 1 cut(s) 626
BstNI CCWGG 1 cut(s) 675
BstNSI RCATGY 2 cut(s) 89, 317
BstSCI CCNGG 1 cut(s) 673
BstSFI CTRYAG 2 cut(s) 120, 437
BstV1I GCAGC 3 cut(s) 91, 209, 677
BstV2I GAAGAC 2 cut(s) 383, 401
BstX2I RGATCY 1 cut(s) 644
BstYI RGATCY 1 cut(s) 644
Bsu15I ATCGAT 1 cut(s) 504
BsuRI GGCC 4 cut(s) 100, 190, 521, 620
BsuTUI ATCGAT 1 cut(s) 504
BtgZI GCGATG 1 cut(s) 419
BtsCI GGATG 4 cut(s) 53, 203, 212, 238
BtsIMutI CAGTG 1 cut(s) 263
Cac8I GCNNGC 2 cut(s) 102, 192
CaiI CAGNNNCTG 1 cut(s) 107
Cfr10I RCCGGY 1 cut(s) 536
Cfr13I GGNCC 1 cut(s) 619
ClaI ATCGAT 1 cut(s) 504
Csp6I GTAC 6 cut(s) 385, 409, 455, 487, 496, 722
CviAII CATG 5 cut(s) 86, 148, 179, 314, 413
CviQI GTAC 6 cut(s) 385, 409, 455, 487, 496, 722
DdeI CTNAG 1 cut(s) 281
DpnI GATC 3 cut(s) 507, 646, 672
DpnII GATC 3 cut(s) 505, 644, 670
DriI GACNNNNNGTC 1 cut(s) 548
EaeI YGGCCR 1 cut(s) 98
Eam1104I CTCTTC 2 cut(s) 214, 578
Eam1105I GACNNNNNGTC 1 cut(s) 548
EarI CTCTTC 2 cut(s) 214, 578
EciI GGCGGA 1 cut(s) 555
Eco130I CCWWGG 1 cut(s) 217
Eco31I GGTCTC 1 cut(s) 188
Eco57I CTGAAG 3 cut(s) 149, 395, 452
EcoNI CCTNNNNNAGG 1 cut(s) 440
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 217
EcoT22I ATGCAT 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 5 cut(s) 89, 151, 182, 317, 416
FatI CATG 5 cut(s) 85, 147, 178, 313, 412
Fnu4HI GCNGC 3 cut(s) 105, 223, 691
FokI GGATG 4 cut(s) 40, 190, 199, 245
Fsp4HI GCNGC 3 cut(s) 105, 223, 691
FspBI CTAG 1 cut(s) 401
GluI GCNGC 3 cut(s) 105, 223, 691
HaeIII GGCC 4 cut(s) 100, 190, 521, 620
HapII CCGG 2 cut(s) 391, 537
Hin1I GRCGYC 1 cut(s) 479
Hin1II CATG 5 cut(s) 89, 151, 182, 317, 416
HinfI GANTC 3 cut(s) 5, 527, 704
HpaII CCGG 2 cut(s) 391, 537
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 171, 602
Hpy188I TCNGA 4 cut(s) 361, 510, 560, 709
Hpy188III TCNNGA 2 cut(s) 373, 391
Hpy8I GTNNAC 2 cut(s) 171, 602
Hpy99I CGWCG 1 cut(s) 484
HpyAV CCTTC 1 cut(s) 436
HpyCH4III ACNGT 3 cut(s) 49, 668, 721
HpyCH4IV ACGT 1 cut(s) 479
HpyCH4V TGCA 6 cut(s) 19, 85, 151, 225, 439, 693
HpyF10VI GCNNNNNNNGC 1 cut(s) 626
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 1 cut(s) 479
Hsp92I GRCGYC 1 cut(s) 479
Hsp92II CATG 5 cut(s) 89, 151, 182, 317, 416
Kpn2I TCCGGA 1 cut(s) 390
Kzo9I GATC 3 cut(s) 505, 644, 670
LguI GCTCTTC 1 cut(s) 578
LmnI GCTCC 3 cut(s) 274, 310, 362
Lsp1109I GCAGC 3 cut(s) 91, 209, 677
LweI GCATC 2 cut(s) 223, 551
MaeI CTAG 1 cut(s) 401
MaeII ACGT 1 cut(s) 479
MaeIII GTNAC 1 cut(s) 43
MalI GATC 3 cut(s) 507, 646, 672
MboI GATC 3 cut(s) 505, 644, 670
MboII GAAGA 7 cut(s) 25, 155, 201, 388, 406, 592, 595
MflI RGATCY 1 cut(s) 644
MhlI GDGCHC 1 cut(s) 359
MlsI TGGCCA 1 cut(s) 100
MluCI AATT 2 cut(s) 320, 491
MluNI TGGCCA 1 cut(s) 100
MlyI GAGTC 2 cut(s) 14, 536
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 2 cut(s) 217, 641
Mox20I TGGCCA 1 cut(s) 100
Mph1103I ATGCAT 1 cut(s) 87
MroI TCCGGA 1 cut(s) 390
MscI TGGCCA 1 cut(s) 100
MseI TTAA 3 cut(s) 240, 608, 699
Msp20I TGGCCA 1 cut(s) 100
MspA1I CMGCKG 2 cut(s) 104, 690
MspI CCGG 2 cut(s) 391, 537
MspR9I CCNGG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 116
MvaI CCWGG 1 cut(s) 675
MwoI GCNNNNNNNGC 1 cut(s) 626
NdeII GATC 3 cut(s) 505, 644, 670
NlaIII CATG 5 cut(s) 89, 151, 182, 317, 416
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 87
NspI RCATGY 2 cut(s) 89, 317
PciSI GCTCTTC 1 cut(s) 578
PctI GAATGC 1 cut(s) 116
PfeI GAWTC 1 cut(s) 704
PkrI GCNGC 3 cut(s) 106, 224, 692
PleI GAGTC 2 cut(s) 13, 535
PpsI GAGTC 2 cut(s) 13, 535
PshBI ATTAAT 1 cut(s) 608
Psp6I CCWGG 1 cut(s) 673
PspGI CCWGG 1 cut(s) 673
PspPI GGNCC 1 cut(s) 619
PstI CTGCAG 1 cut(s) 441
PstNI CAGNNNCTG 1 cut(s) 107
PsuI RGATCY 1 cut(s) 644
PvuII CAGCTG 2 cut(s) 104, 690
RsaI GTAC 6 cut(s) 386, 410, 456, 488, 497, 723
RsaNI GTAC 6 cut(s) 385, 409, 455, 487, 496, 722
SapI GCTCTTC 1 cut(s) 578
SaqAI TTAA 3 cut(s) 240, 608, 699
SatI GCNGC 3 cut(s) 105, 223, 691
Sau3AI GATC 3 cut(s) 505, 644, 670
Sau96I GGNCC 1 cut(s) 619
ScaI AGTACT 1 cut(s) 386
SchI GAGTC 2 cut(s) 14, 536
ScrFI CCNGG 1 cut(s) 675
SduI GDGCHC 1 cut(s) 359
SetI ASST 7 cut(s) 106, 121, 279, 430, 482, 692, 747
SfaNI GCATC 2 cut(s) 223, 551
SfcI CTRYAG 2 cut(s) 120, 437
Sse9I AATT 2 cut(s) 320, 491
SsiI CCGC 2 cut(s) 57, 540
SspI AATATT 1 cut(s) 451
SspMI CTAG 1 cut(s) 401
StyD4I CCNGG 1 cut(s) 673
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 3 cut(s) 49, 668, 721
TaiI ACGT 1 cut(s) 482
TaqI TCGA 3 cut(s) 8, 318, 504
TaqII GACCGA 1 cut(s) 395
TasI AATT 2 cut(s) 320, 491
TatI WGTACW 3 cut(s) 384, 486, 495
TfiI GAWTC 1 cut(s) 704
Tru1I TTAA 3 cut(s) 240, 608, 699
Tru9I TTAA 3 cut(s) 240, 608, 699
TscAI CASTG 1 cut(s) 270
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 3 cut(s) 104, 222, 690
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 5 cut(s) 42, 167, 201, 269, 717
TspGWI ACGGA 2 cut(s) 363, 538
TspRI CASTG 1 cut(s) 270
VspI ATTAAT 1 cut(s) 608
XagI CCTNNNNNAGG 1 cut(s) 440
XapI RAATTY 1 cut(s) 320
XceI RCATGY 2 cut(s) 89, 317
XspI CTAG 1 cut(s) 401
ZraI GACGTC 1 cut(s) 480
ZrmI AGTACT 1 cut(s) 386
Zsp2I ATGCAT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.