RLG00000025222

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000345
Physical Location & Seq
Reverse (-)
43094399 .. 43095167
769 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025222

Sequence Viewer

Length: 654 bp
ATGGGGATCATGCAACTCGTCCTGCTTCTTCTCTCTGCGTGGGGGGCAGCTGATGCAGCCAATCCCTACGACTACCTCCAATTTGTCCAGCAGTGGCCTGCTACATTCTGTTCCGGCAGGTCAGATTGTATCCCAAATCCCCCGCAGAATTACTTTACTATCCACGGGCTTTGGCCATCCAATTACTCGACTCCTGGACAGGATTGCCATGGCACACCTTTTAGTGGAGCTGAGATGTCTGCTACCGAAAACCATGGTTTGAGGTACTATTTTCTGCCGGTAGCATGGCCCCAATTGATAGCTCATTATTCCGATATGGACTTCTGGGAAACAGAGTACACTAGGCATGGTACTTGCTCGGAGGACAATCTGAACCAGACGGAATATTTTAAGAAAGCTTACTGGATGTGGTATCAATATAATGCATATCAGCTGTTTGCAATCGCACCGTCACCAATCTACCCTGGAAATTACTATTATCGCATTGACCTTGAAAATGCCATTCAGCGAGTGACTGTATACCAGCCTGTGCTTAGGTGCCGGCAGATAAGAGTAGGTAGAATTGATGTTTGGTATTTGGAAGAAGTCATCATTGCTTTGATGATATGGGAAACAACAGTGTCCCTTGTGGACGACGGACAAATTGCGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

218

Amino Acids

25.24

Weight (kDa)

4.74

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 23 - 196 5e-31 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 108
AccB1I GGYRCC 1 cut(s) 537
AccI GTMKAC 1 cut(s) 519
AciI CCGC 2 cut(s) 143, 647
AclWI GGATC 1 cut(s) 14
AcoI YGGCCR 1 cut(s) 173
AfaI GTAC 3 cut(s) 266, 338, 352
AfiI CCNNNNNNNGG 1 cut(s) 224
AgsI TTSAA 1 cut(s) 494
AjnI CCWGG 2 cut(s) 193, 463
AjuI GAANNNNNNNTTGG 2 cut(s) 553, 585
AluBI AGCT 5 cut(s) 50, 230, 302, 398, 433
AluI AGCT 5 cut(s) 50, 230, 302, 398, 433
AlwI GGATC 1 cut(s) 14
AoxI GGCC 3 cut(s) 95, 173, 287
ApeKI GCWGC 2 cut(s) 47, 56
AspS9I GGNCC 1 cut(s) 288
AsuHPI GGTGA 1 cut(s) 444
BalI TGGCCA 1 cut(s) 175
BanI GGYRCC 1 cut(s) 537
BbvI GCAGC 2 cut(s) 59, 68
BccI CCATC 1 cut(s) 184
BciT130I CCWGG 2 cut(s) 195, 465
BciVI GTATCC 1 cut(s) 140
BfaI CTAG 1 cut(s) 342
BfuAI ACCTGC 1 cut(s) 108
BfuI GTATCC 1 cut(s) 140
BisI GCNGC 2 cut(s) 48, 57
BlsI GCNGC 2 cut(s) 49, 58
Bme1390I CCNGG 2 cut(s) 195, 465
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 2 cut(s) 290, 539
BmrFI CCNGG 2 cut(s) 195, 465
BmsI GCATC 1 cut(s) 43
Bpu10I CCTNAGC 1 cut(s) 533
BsaJI CCNNGG 4 cut(s) 163, 208, 253, 463
Bsc4I CCNNNNNNNGG 1 cut(s) 224
Bse118I RCCGGY 2 cut(s) 277, 540
Bse1I ACTGG 1 cut(s) 407
Bse3DI GCAATG 1 cut(s) 591
BseBI CCWGG 2 cut(s) 195, 465
BseDI CCNNGG 4 cut(s) 163, 208, 253, 463
BseGI GGATG 2 cut(s) 176, 411
BseLI CCNNNNNNNGG 1 cut(s) 224
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 407
BseXI GCAGC 2 cut(s) 59, 68
BshFI GGCC 3 cut(s) 97, 175, 289
BshNI GGYRCC 1 cut(s) 537
BsiSI CCGG 3 cut(s) 114, 278, 541
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 1 cut(s) 224
BsmFI GGGAC 1 cut(s) 607
BsnI GGCC 3 cut(s) 97, 175, 289
Bsp143I GATC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 208, 253
BspACI CCGC 2 cut(s) 143, 647
BspANI GGCC 3 cut(s) 97, 175, 289
BspCNI CTCAG 1 cut(s) 223
BspLI GGNNCC 2 cut(s) 290, 539
BspMI ACCTGC 1 cut(s) 108
BspPI GGATC 1 cut(s) 14
BspT107I GGYRCC 1 cut(s) 537
BsrDI GCAATG 1 cut(s) 591
BsrFI RCCGGY 2 cut(s) 277, 540
BsrI ACTGG 1 cut(s) 407
BssAI RCCGGY 2 cut(s) 277, 540
BssECI CCNNGG 4 cut(s) 163, 208, 253, 463
BssMI GATC 1 cut(s) 6
BssNAI GTATAC 1 cut(s) 520
BssT1I CCWWGG 2 cut(s) 208, 253
Bst1107I GTATAC 1 cut(s) 520
Bst2UI CCWGG 2 cut(s) 195, 465
Bst4CI ACNGT 3 cut(s) 450, 517, 619
BstAPI GCANNNNNTGC 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 99, 542
BstDEI CTNAG 2 cut(s) 231, 533
BstDSI CCRYGG 3 cut(s) 163, 208, 253
BstF5I GGATG 2 cut(s) 176, 411
BstKTI GATC 1 cut(s) 9
BstMBI GATC 1 cut(s) 6
BstMWI GCNNNNNNNGC 3 cut(s) 44, 53, 56
BstNI CCWGG 2 cut(s) 195, 465
BstSCI CCNGG 2 cut(s) 193, 463
BstV1I GCAGC 2 cut(s) 59, 68
BstZ17I GTATAC 1 cut(s) 520
BsuI GTATCC 1 cut(s) 140
BsuRI GGCC 3 cut(s) 97, 175, 289
BtgI CCRYGG 3 cut(s) 163, 208, 253
BtsCI GGATG 2 cut(s) 176, 411
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 2 cut(s) 98, 624
BveI ACCTGC 1 cut(s) 108
Cac8I GCNNGC 2 cut(s) 99, 542
Cfr10I RCCGGY 2 cut(s) 277, 540
Cfr13I GGNCC 1 cut(s) 288
Csp6I GTAC 3 cut(s) 265, 337, 351
CspCI CAANNNNNGTGG 2 cut(s) 152, 187
CviAII CATG 5 cut(s) 10, 209, 254, 285, 347
CviQI GTAC 3 cut(s) 265, 337, 351
DdeI CTNAG 2 cut(s) 231, 533
DpnI GATC 1 cut(s) 8
DpnII GATC 1 cut(s) 6
EaeI YGGCCR 1 cut(s) 173
Eco130I CCWWGG 2 cut(s) 208, 253
EcoRII CCWGG 2 cut(s) 193, 463
EcoT14I CCWWGG 2 cut(s) 208, 253
EcoT22I ATGCAT 1 cut(s) 427
ErhI CCWWGG 2 cut(s) 208, 253
FaeI CATG 5 cut(s) 13, 212, 257, 288, 350
FaqI GGGAC 1 cut(s) 607
FatI CATG 5 cut(s) 9, 208, 253, 284, 346
FauI CCCGC 1 cut(s) 150
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 2 cut(s) 48, 57
FokI GGATG 2 cut(s) 163, 418
Fsp4HI GCNGC 2 cut(s) 48, 57
FspBI CTAG 1 cut(s) 342
GluI GCNGC 2 cut(s) 48, 57
HaeIII GGCC 3 cut(s) 97, 175, 289
HapII CCGG 3 cut(s) 114, 278, 541
Hin1II CATG 5 cut(s) 13, 212, 257, 288, 350
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 1 cut(s) 190
HpaII CCGG 3 cut(s) 114, 278, 541
HphI GGTGA 1 cut(s) 444
Hpy166II GTNNAC 3 cut(s) 339, 520, 631
Hpy188I TCNGA 4 cut(s) 124, 313, 361, 372
Hpy8I GTNNAC 3 cut(s) 339, 520, 631
Hpy99I CGWCG 1 cut(s) 638
HpyCH4III ACNGT 3 cut(s) 450, 517, 619
HpyCH4V TGCA 4 cut(s) 13, 56, 425, 440
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 53, 56
HpyF3I CTNAG 2 cut(s) 231, 533
Hsp92II CATG 5 cut(s) 13, 212, 257, 288, 350
KroI GCCGGC 1 cut(s) 540
KroNI GCCGGC 1 cut(s) 542
Kzo9I GATC 1 cut(s) 6
LmnI GCTCC 1 cut(s) 227
Lsp1109I GCAGC 2 cut(s) 59, 68
LweI GCATC 1 cut(s) 43
MaeI CTAG 1 cut(s) 342
MaeIII GTNAC 2 cut(s) 450, 511
MalI GATC 1 cut(s) 8
MboI GATC 1 cut(s) 6
MboII GAAGA 2 cut(s) 20, 593
MfeI CAATTG 1 cut(s) 293
MlsI TGGCCA 1 cut(s) 175
MluCI AATT 7 cut(s) 80, 148, 181, 293, 469, 561, 642
MluNI TGGCCA 1 cut(s) 175
MlyI GAGTC 1 cut(s) 184
MnlI CCTC 3 cut(s) 86, 255, 355
Mox20I TGGCCA 1 cut(s) 175
Mph1103I ATGCAT 1 cut(s) 427
MroNI GCCGGC 1 cut(s) 540
MscI TGGCCA 1 cut(s) 175
MseI TTAA 1 cut(s) 390
Msp20I TGGCCA 1 cut(s) 175
MspA1I CMGCKG 2 cut(s) 50, 433
MspI CCGG 3 cut(s) 114, 278, 541
MspR9I CCNGG 2 cut(s) 195, 465
MunI CAATTG 1 cut(s) 293
MvaI CCWGG 2 cut(s) 195, 465
MwoI GCNNNNNNNGC 3 cut(s) 44, 53, 56
NaeI GCCGGC 1 cut(s) 542
NcoI CCATGG 2 cut(s) 208, 253
NdeII GATC 1 cut(s) 6
NgoMIV GCCGGC 1 cut(s) 540
NlaIII CATG 5 cut(s) 13, 212, 257, 288, 350
NlaIV GGNNCC 2 cut(s) 290, 539
NmuCI GTSAC 2 cut(s) 450, 511
NsiI ATGCAT 1 cut(s) 427
PdiI GCCGGC 1 cut(s) 542
PfoI TCCNGGA 1 cut(s) 193
PkrI GCNGC 2 cut(s) 49, 58
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 193, 463
PspGI CCWGG 2 cut(s) 193, 463
PspN4I GGNNCC 2 cut(s) 290, 539
PspPI GGNCC 1 cut(s) 288
PvuII CAGCTG 2 cut(s) 50, 433
RsaI GTAC 3 cut(s) 266, 338, 352
RsaNI GTAC 3 cut(s) 265, 337, 351
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 2 cut(s) 48, 57
Sau3AI GATC 1 cut(s) 6
Sau96I GGNCC 1 cut(s) 288
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 2 cut(s) 195, 465
SfaNI GCATC 1 cut(s) 43
Sse9I AATT 7 cut(s) 80, 148, 181, 293, 469, 561, 642
SsiI CCGC 2 cut(s) 143, 647
SspI AATATT 1 cut(s) 386
SspMI CTAG 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 193, 463
StyI CCWWGG 2 cut(s) 208, 253
TaaI ACNGT 3 cut(s) 450, 517, 619
TaqI TCGA 1 cut(s) 188
TasI AATT 7 cut(s) 80, 148, 181, 293, 469, 561, 642
TatI WGTACW 1 cut(s) 336
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 2 cut(s) 98, 624
TseFI GTSAC 2 cut(s) 450, 511
TseI GCWGC 2 cut(s) 47, 56
Tsp45I GTSAC 2 cut(s) 450, 511
TspGWI ACGGA 2 cut(s) 395, 651
TspRI CASTG 2 cut(s) 98, 624
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 1 cut(s) 342
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.