pycom04g16990

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000345
Physical Location & Seq
Reverse (-)
19523629 .. 19524450
822 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g16990.2

Sequence Viewer

Length: 711 bp
ATGTCATCGCTATTATTCCATTTATACATTCTCCTTGCAATATTAGTATTATACAATTTATACACACACATTGCAATATTTCATCTTCCAGTTTCCTCCGTCTTCCCCACTCAAACTTTGGGTGCCACACCATACAAGTTTCTCATGTTCACACAGCAGTGGCCGAAAGCTGTGAACAATAGTAGCCGATTGACCACTTTTACAATCCACGGCCTGTGGCCGTCAAATGGTTCCGCAGTGCTGATTTGCAAGGGAACAAATTTTACTAGAAGCAAGATGACGACTGGCCTAGAAGGGAAACTCAATATCTCTTGGCCGAATCTCAAAGGTGATAATAATACGCGGTTTTGGGAATACGAGTACAATAATCACGGTACGTGTTCCGAAGATACCTTCGGGTACAACCAGACCAAATACTTTGAGCTCGCCAACGAGATGTGGGGAAAATTGAACATAGCTCAAATGCTTAAAAAGTGGAAAATCGTTCCAGGTGCAAATTATACCACATCTCAATTTGTGGCTGCCATTAAGAAAGAAAATCAGAATAAATCACCCTCCATTCGTTGTAAAAATGGATCAACAGAGTCAGAGGTATTATTGCTTGAGGTGGTTGTATGTTACGACCACTACGGAAAAAAAGTGATCGACTGCAAGCATAACATGAGCAGCAGCTGCAGGTCTCAGAATCAGAACATATCATACGTGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

237

Amino Acids

26.94

Weight (kDa)

9.29

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000345)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g19170 FvH4_6g07690 FvH4_6g07740 FvH4_6g07750 FvH4_6g08450
malus_domestica MD01G1175200.v1.1 MD04G1192300.v1.1 MD10G1162000.v1.1 MD10G1162800.v1.1 MD15G1167100.v1.1 MD15G1167600.v1.1 MD15G1167800.v1.1
prunus_persica Prupe.6G319200_v2.0.a1 Prupe.8G219600_v2.0.a1
pyrus_communis pycom04g16990 pycom10g14020 pycom15g14910
rosa_chinensis RchiOBHm_Chr2g0110251 RchiOBHm_Chr2g0111581 RchiOBHm_Chr2g0111611 RchiOBHm_Chr2g0123571 RchiOBHm_Chr3g0455911 RchiOBHm_Chr3g0457381 RchiOBHm_Chr3g0457441 RchiOBHm_Chr3g0458491 RchiOBHm_Chr3g0491491 RchiOBHm_Chr3g0491511 RchiOBHm_Chr4g0398371 RchiOBHm_Chr6g0286961 RchiOBHm_Chr6g0293901
rosa_laevigata RLG00000012522 RLG00000015565 RLG00000017900 RLG00000017902 RLG00000017904 RLG00000017906 RLG00000017908 RLG00000017910 RLG00000017912 RLG00000017914 RLG00000024924 RLG00000025153 RLG00000025197 RLG00000025222 RLG00000025224 RLG00000025227 RLG00000025231 RLG00000025323
rosa_multiflora Rmu_sc0004094.1_g000043 Rmu_sc0005442.1_g000003 Rmu_sc0005791.1_g000016 Rmu_sc0006034.1_g000014 Rmu_sc0008973.1_g000002 Rmu_sc0008973.1_g000014 Rmu_sc0008973.1_g000018 Rmu_sc0013809.1_g000009 Rmu_ssc0000091.1_g000008
rosa_roxburghii Rroxscaffold_2G00132220 Rroxscaffold_6G00418220 Rroxscaffold_6G00418550 Rroxscaffold_6G00421070
rosa_rugosa Rorug02G0174900 Rorug02G0461000 Rorug03G0020400 Rorug03G0028600 Rorug03G0029200 Rorug06G0126000 Rorug06G0185700
rosa_samantha Rh2AG218200 Rh2AG218400 Rh2AG227600 Rh2AG305200 Rh2BG239900 Rh2BG313300 Rh2CG220000 Rh2CG220300 Rh2CG291400 Rh2DG224000 Rh2DG224200 Rh2DG329100 Rh3AG071200 Rh3AG080700 Rh3AG081000 Rh3AG298900 Rh3BG073600 Rh3BG082600 Rh3BG083100 Rh3CG072200 Rh3CG082800 Rh3CG083300 Rh3CG331700 Rh3DG074900 Rh3DG080700 Rh3DG084000 Rh3DG084500 Rh3DG091600 Rh3DG333300 Rh4AG085900 Rh4CG093600 Rh4DG077700 Rh6AG352300 Rh6CG366500
rosa_wichuraiana Rw2G017590 Rw3G005760 Rw3G006100 Rw3G007480 Rw3G026400 Rw4G007170 Rw6G030670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 666
AccB1I GGYRCC 1 cut(s) 122
AccII CGCG 1 cut(s) 343
AciI CCGC 2 cut(s) 234, 343
AclWI GGATC 1 cut(s) 583
AcoI YGGCCR 3 cut(s) 161, 218, 314
AcsI RAATTY 1 cut(s) 259
AfaI GTAC 3 cut(s) 362, 376, 401
AfiI CCNNNNNNNGG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 377
AgsI TTSAA 1 cut(s) 451
AjnI CCWGG 1 cut(s) 487
AleI CACNNNNGTG 1 cut(s) 157
AluBI AGCT 4 cut(s) 170, 424, 458, 672
AluI AGCT 4 cut(s) 170, 424, 458, 672
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 1 cut(s) 583
AlwNI CAGNNNCTG 1 cut(s) 672
AoxI GGCC 5 cut(s) 161, 211, 218, 286, 314
ApeKI GCWGC 4 cut(s) 521, 666, 669, 672
ApoI RAATTY 1 cut(s) 259
AsuHPI GGTGA 2 cut(s) 341, 543
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 426
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 4 cut(s) 508, 659, 678, 681
BceAI ACGGC 2 cut(s) 205, 226
BciT130I CCWGG 1 cut(s) 489
BcoDI GTCTC 1 cut(s) 684
BfaI CTAG 2 cut(s) 267, 290
BfmI CTRYAG 1 cut(s) 673
BfuAI ACCTGC 1 cut(s) 666
BisI GCNGC 4 cut(s) 522, 667, 670, 673
BlsI GCNGC 4 cut(s) 523, 668, 671, 674
Bme1390I CCNGG 1 cut(s) 489
BmiI GGNNCC 2 cut(s) 124, 232
BmrFI CCNGG 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 94
BpuEI CTTGAG 1 cut(s) 623
BsaAI YACGTR 2 cut(s) 378, 703
BsaI GGTCTC 1 cut(s) 684
BsaJI CCNNGG 1 cut(s) 208
Bsc4I CCNNNNNNNGG 1 cut(s) 227
Bse1I ACTGG 2 cut(s) 89, 289
Bse3DI GCAATG 1 cut(s) 69
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 208
BseLI CCNNNNNNNGG 1 cut(s) 227
BseMI GCAATG 1 cut(s) 69
BseMII CTCAG 1 cut(s) 695
BseNI ACTGG 2 cut(s) 89, 289
BseXI GCAGC 4 cut(s) 508, 659, 678, 681
Bsh1236I CGCG 1 cut(s) 343
BshFI GGCC 5 cut(s) 163, 213, 220, 288, 316
BshNI GGYRCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 426
BslI CCNNNNNNNGG 1 cut(s) 227
BsmAI GTCTC 1 cut(s) 684
BsnI GGCC 5 cut(s) 163, 213, 220, 288, 316
Bso31I GGTCTC 1 cut(s) 684
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 2 cut(s) 575, 642
BspACI CCGC 2 cut(s) 234, 343
BspANI GGCC 5 cut(s) 163, 213, 220, 288, 316
BspCNI CTCAG 1 cut(s) 694
BspFNI CGCG 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 124, 232
BspMAI CTGCAG 1 cut(s) 677
BspMI ACCTGC 1 cut(s) 666
BspPI GGATC 1 cut(s) 583
BspT107I GGYRCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 684
BsrDI GCAATG 1 cut(s) 69
BsrI ACTGG 2 cut(s) 89, 289
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 2 cut(s) 575, 642
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 1 cut(s) 374
BstAPI GCANNNNNTGC 1 cut(s) 672
BstBAI YACGTR 2 cut(s) 378, 703
BstC8I GCNNGC 2 cut(s) 426, 653
BstDEI CTNAG 1 cut(s) 681
BstDSI CCRYGG 1 cut(s) 208
BstFNI CGCG 1 cut(s) 343
BstKTI GATC 2 cut(s) 578, 645
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 2 cut(s) 575, 642
BstMWI GCNNNNNNNGC 1 cut(s) 672
BstNI CCWGG 1 cut(s) 489
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 673
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 4 cut(s) 508, 659, 678, 681
BstV2I GAAGAC 1 cut(s) 94
BsuRI GGCC 5 cut(s) 163, 213, 220, 288, 316
BtgI CCRYGG 1 cut(s) 208
BtsI GCAGTG 2 cut(s) 164, 243
BtsIMutI CAGTG 2 cut(s) 164, 243
BveI ACCTGC 1 cut(s) 666
Cac8I GCNNGC 2 cut(s) 426, 653
CaiI CAGNNNCTG 1 cut(s) 672
Csp6I GTAC 3 cut(s) 361, 375, 400
CviAII CATG 2 cut(s) 145, 661
CviQI GTAC 3 cut(s) 361, 375, 400
DdeI CTNAG 1 cut(s) 681
DpnI GATC 2 cut(s) 577, 644
DpnII GATC 2 cut(s) 575, 642
EaeI YGGCCR 3 cut(s) 161, 218, 314
Ecl136II GAGCTC 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 426
Eco31I GGTCTC 1 cut(s) 684
Eco53kI GAGCTC 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 487
EcoT38I GRGCYC 1 cut(s) 426
FaeI CATG 2 cut(s) 148, 664
FatI CATG 2 cut(s) 144, 660
Fnu4HI GCNGC 4 cut(s) 522, 667, 670, 673
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 4 cut(s) 522, 667, 670, 673
FspBI CTAG 2 cut(s) 267, 290
GluI GCNGC 4 cut(s) 522, 667, 670, 673
HaeIII GGCC 5 cut(s) 163, 213, 220, 288, 316
Hin1II CATG 2 cut(s) 148, 664
HinfI GANTC 3 cut(s) 319, 584, 685
HphI GGTGA 2 cut(s) 341, 543
Hpy166II GTNNAC 2 cut(s) 150, 175
Hpy188I TCNGA 5 cut(s) 385, 543, 589, 684, 690
Hpy8I GTNNAC 2 cut(s) 150, 175
HpyAV CCTTC 2 cut(s) 287, 403
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 2 cut(s) 377, 702
HpyCH4V TGCA 6 cut(s) 38, 74, 249, 494, 651, 675
HpyF10VI GCNNNNNNNGC 1 cut(s) 672
HpyF3I CTNAG 1 cut(s) 681
HpySE526I ACGT 2 cut(s) 377, 702
Hsp92II CATG 2 cut(s) 148, 664
Kzo9I GATC 2 cut(s) 575, 642
LpnPI CCDG 7 cut(s) 102, 227, 270, 419, 474, 501, 661
Lsp1109I GCAGC 4 cut(s) 508, 659, 678, 681
MaeI CTAG 2 cut(s) 267, 290
MaeII ACGT 2 cut(s) 377, 702
MaeIII GTNAC 1 cut(s) 617
MalI GATC 2 cut(s) 577, 644
MboI GATC 2 cut(s) 575, 642
MboII GAAGA 3 cut(s) 77, 94, 398
MhlI GDGCHC 1 cut(s) 426
MluCI AATT 5 cut(s) 55, 259, 446, 496, 512
MlyI GAGTC 1 cut(s) 593
MnlI CCTC 4 cut(s) 106, 565, 583, 598
MseI TTAA 2 cut(s) 468, 528
MslI CAYNNNNRTG 1 cut(s) 157
MspA1I CMGCKG 1 cut(s) 672
MspR9I CCNGG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 489
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 1 cut(s) 672
NdeII GATC 2 cut(s) 575, 642
NlaIII CATG 2 cut(s) 148, 664
NlaIV GGNNCC 2 cut(s) 124, 232
OliI CACNNNNGTG 1 cut(s) 157
PfeI GAWTC 2 cut(s) 319, 685
PkrI GCNGC 4 cut(s) 523, 668, 671, 674
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
Ppu21I YACGTR 2 cut(s) 378, 703
Psp124BI GAGCTC 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 2 cut(s) 124, 232
PsrI GAACNNNNNNTAC 3 cut(s) 247, 279, 683
PstI CTGCAG 1 cut(s) 677
PstNI CAGNNNCTG 1 cut(s) 672
PvuII CAGCTG 1 cut(s) 672
RsaI GTAC 3 cut(s) 362, 376, 401
RsaNI GTAC 3 cut(s) 361, 375, 400
RseI CAYNNNNRTG 1 cut(s) 157
SacI GAGCTC 1 cut(s) 426
SaqAI TTAA 2 cut(s) 468, 528
SatI GCNGC 4 cut(s) 522, 667, 670, 673
Sau3AI GATC 2 cut(s) 575, 642
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 1 cut(s) 426
SfcI CTRYAG 1 cut(s) 673
SmiMI CAYNNNNRTG 1 cut(s) 157
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
Sse9I AATT 5 cut(s) 55, 259, 446, 496, 512
SsiI CCGC 2 cut(s) 234, 343
SspI AATATT 2 cut(s) 42, 78
SspMI CTAG 2 cut(s) 267, 290
SstI GAGCTC 1 cut(s) 426
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 2 cut(s) 380, 705
TaqI TCGA 1 cut(s) 645
TasI AATT 5 cut(s) 55, 259, 446, 496, 512
TatI WGTACW 1 cut(s) 360
TfiI GAWTC 2 cut(s) 319, 685
Tru1I TTAA 2 cut(s) 468, 528
Tru9I TTAA 2 cut(s) 468, 528
TscAI CASTG 2 cut(s) 164, 243
TseI GCWGC 4 cut(s) 521, 666, 669, 672
TspDTI ATGAA 1 cut(s) 71
TspGWI ACGGA 2 cut(s) 88, 645
TspRI CASTG 2 cut(s) 164, 243
XapI RAATTY 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XspI CTAG 2 cut(s) 267, 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.