AT5G28730
MYB Family

nuclease HARBI1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000469
Physical Location & Seq
Forward (+)
10779003 .. 10780218
1216 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28730.1

Sequence Viewer

Length: 891 bp
ATGTTACTAGAAGAAGAAGCGAATATGGAAGAATGCATAGCTCATCAAATTTACAGTAATGAAGTTTCATGTCAGACACTTATCCGAATGAGTTCAGAGGCATTCACACAGTTATGTGAAATATTACATGGGAAGTATGGTTTACAAAGCTCGACGAACATAAGCCTCGACGAGAGTGTTGCAATCTTTCTAATCATTTGTGCCTCAAATGATACACAACGTGATATTGCATTAAGGTTTGGACATGCCCAAGAGACAATTTGGCGAAAGTTTCATGATGTGCTTAAAGCAATGGAACGATTGGCTGTAGAGTATATACGCCCAAGAAAAGTCGAAGAACTTAGAGCTATCTCTAATCGTTTGCAAGATGATACAAGATATTGGCCATTTTTAATGGATTTATTGGGAATTGCAAGCTTCAATGTTCTAGCTATATGCGATCTTGACATGCTTTTCACATATTGTTTTGTCGGGATGGCAGGATCTACCCATGATGCACGTGTCCTTTCGGCTGCGATAAGTGATGATCCACTATTTCATGTACCTCCAGATAGTAAGTATTATCTTGTAGATTCTGGATATGCTAATAAACGTGGATATTTAGCTCCATACCGAAGAGAACATAGAGAGGCACAAGATATCATCTCCAATAATTTTTTAACGGTGAACCTCTTCGAAACACATAATATTAAAGACTATGATTTTGACAATGTTGATTCTGAGAATAATGTTGTACAAGCTCCACATGATGCGACAGATGAGCAAGATAACAATGATGGTATTGAAATTGAAGAATCATCAGAGGCAGAAATGTATATGCGGATAGTTCGTGATCAAATTGCTGAACACATATGGTCAGTAAAAGGATATGGATCTCGACGTTGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

296

Amino Acids

34.1

Weight (kDa)

4.79

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 10 - 98 8e-23 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 139 - 213 1.5e-07 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 820
AclWI GGATC 3 cut(s) 490, 521, 880
AcoI YGGCCR 1 cut(s) 383
AcsI RAATTY 1 cut(s) 48
AcvI CACGTG 1 cut(s) 500
AdeI CACNNNGTG 1 cut(s) 221
AfaI GTAC 2 cut(s) 543, 735
AflIII ACRYGT 1 cut(s) 499
AgsI TTSAA 3 cut(s) 421, 785, 791
AluBI AGCT 7 cut(s) 41, 150, 347, 417, 431, 605, 740
AluI AGCT 7 cut(s) 41, 150, 347, 417, 431, 605, 740
Alw26I GTCTC 1 cut(s) 248
AlwI GGATC 3 cut(s) 490, 521, 880
AoxI GGCC 1 cut(s) 383
ApeKI GCWGC 1 cut(s) 512
ApoI RAATTY 1 cut(s) 48
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
Asp700I GAANNNNTTC 2 cut(s) 91, 671
AsuHPI GGTGA 1 cut(s) 676
AsuII TTCGAA 1 cut(s) 675
BalI TGGCCA 1 cut(s) 385
BbrPI CACGTG 1 cut(s) 500
BbvI GCAGC 1 cut(s) 499
BccI CCATC 2 cut(s) 469, 770
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BclI TGATCA 1 cut(s) 832
BcoDI GTCTC 1 cut(s) 248
BfaI CTAG 2 cut(s) 8, 428
BfmI CTRYAG 1 cut(s) 306
BisI GCNGC 1 cut(s) 513
BlsI GCNGC 1 cut(s) 514
BmsI GCATC 2 cut(s) 484, 739
BpmI CTGGAG 1 cut(s) 531
Bpu14I TTCGAA 1 cut(s) 675
BsaAI YACGTR 1 cut(s) 500
BsaBI GATNNNNATC 1 cut(s) 871
Bse3DI GCAATG 1 cut(s) 297
Bse8I GATNNNNATC 1 cut(s) 871
BseGI GGATG 1 cut(s) 480
BseJI GATNNNNATC 1 cut(s) 871
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 711
BseXI GCAGC 1 cut(s) 499
BshFI GGCC 1 cut(s) 385
BsmAI GTCTC 1 cut(s) 248
BsmI GAATGC 2 cut(s) 38, 101
BsnI GGCC 1 cut(s) 385
Bsp119I TTCGAA 1 cut(s) 675
Bsp1407I TGTACA 1 cut(s) 733
Bsp143I GATC 5 cut(s) 439, 482, 526, 832, 872
BspACI CCGC 1 cut(s) 820
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 1 cut(s) 712
BspHI TCATGA 1 cut(s) 274
BspPI GGATC 3 cut(s) 490, 521, 880
BspT104I TTCGAA 1 cut(s) 675
BsrDI GCAATG 1 cut(s) 297
BsrGI TGTACA 1 cut(s) 733
BssMI GATC 5 cut(s) 439, 482, 526, 832, 872
Bst4CI ACNGT 3 cut(s) 56, 111, 664
Bst6I CTCTTC 2 cut(s) 610, 677
BstAUI TGTACA 1 cut(s) 733
BstBAI YACGTR 1 cut(s) 500
BstBI TTCGAA 1 cut(s) 675
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 2 cut(s) 341, 720
BstF5I GGATG 1 cut(s) 480
BstKTI GATC 5 cut(s) 442, 485, 529, 835, 875
BstMAI GTCTC 1 cut(s) 248
BstMBI GATC 5 cut(s) 439, 482, 526, 832, 872
BstNSI RCATGY 2 cut(s) 248, 451
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 499
BstX2I RGATCY 2 cut(s) 482, 872
BstYI RGATCY 2 cut(s) 482, 872
BsuRI GGCC 1 cut(s) 385
BtsCI GGATG 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 415
CciI TCATGA 1 cut(s) 274
Csp6I GTAC 2 cut(s) 542, 734
CviAII CATG 8 cut(s) 69, 128, 245, 275, 448, 491, 539, 746
CviQI GTAC 2 cut(s) 542, 734
DdeI CTNAG 2 cut(s) 341, 720
DpnI GATC 5 cut(s) 441, 484, 528, 834, 874
DpnII GATC 5 cut(s) 439, 482, 526, 832, 872
DraIII CACNNNGTG 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 383
Eam1104I CTCTTC 2 cut(s) 610, 677
EarI CTCTTC 2 cut(s) 610, 677
Eco32I GATATC 1 cut(s) 640
Eco72I CACGTG 1 cut(s) 500
EcoRV GATATC 1 cut(s) 640
EcoT22I ATGCAT 1 cut(s) 38
FaeI CATG 8 cut(s) 72, 131, 248, 278, 451, 494, 542, 749
FatI CATG 8 cut(s) 68, 127, 244, 274, 447, 490, 538, 745
FauNDI CATATG 1 cut(s) 851
FbaI TGATCA 1 cut(s) 832
Fnu4HI GCNGC 1 cut(s) 513
FokI GGATG 1 cut(s) 487
Fsp4HI GCNGC 1 cut(s) 513
FspBI CTAG 2 cut(s) 8, 428
GluI GCNGC 1 cut(s) 513
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 1 cut(s) 385
Hin1II CATG 8 cut(s) 72, 131, 248, 278, 451, 494, 542, 749
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 3 cut(s) 572, 716, 794
HphI GGTGA 1 cut(s) 676
Hpy166II GTNNAC 2 cut(s) 143, 667
Hpy188I TCNGA 5 cut(s) 75, 86, 97, 721, 802
Hpy188III TCNNGA 7 cut(s) 275, 443, 472, 548, 576, 830, 876
Hpy8I GTNNAC 2 cut(s) 143, 667
Hpy99I CGWCG 3 cut(s) 157, 173, 882
HpyCH4III ACNGT 3 cut(s) 56, 111, 664
HpyCH4IV ACGT 4 cut(s) 220, 499, 592, 880
HpyCH4V TGCA 6 cut(s) 36, 182, 230, 364, 413, 497
HpyF3I CTNAG 2 cut(s) 341, 720
HpySE526I ACGT 4 cut(s) 220, 499, 592, 880
Hsp92II CATG 8 cut(s) 72, 131, 248, 278, 451, 494, 542, 749
Ksp22I TGATCA 1 cut(s) 832
Kzo9I GATC 5 cut(s) 439, 482, 526, 832, 872
LmnI GCTCC 2 cut(s) 610, 745
LpnPI CCDG 3 cut(s) 465, 561, 561
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 2 cut(s) 484, 739
MaeI CTAG 2 cut(s) 8, 428
MaeII ACGT 4 cut(s) 220, 499, 592, 880
MaeIII GTNAC 1 cut(s) 3
MalI GATC 5 cut(s) 441, 484, 528, 834, 874
MboI GATC 5 cut(s) 439, 482, 526, 832, 872
MboII GAAGA 7 cut(s) 23, 26, 41, 347, 627, 664, 803
MflI RGATCY 2 cut(s) 482, 872
MlsI TGGCCA 1 cut(s) 385
MluCI AATT 6 cut(s) 48, 258, 408, 652, 786, 837
MluNI TGGCCA 1 cut(s) 385
MnlI CCTC 7 cut(s) 91, 176, 214, 555, 622, 680, 796
Mox20I TGGCCA 1 cut(s) 385
Mph1103I ATGCAT 1 cut(s) 38
MroXI GAANNNNTTC 2 cut(s) 91, 671
MscI TGGCCA 1 cut(s) 385
MseI TTAA 5 cut(s) 233, 285, 392, 659, 690
MslI CAYNNNNRTG 1 cut(s) 112
Msp20I TGGCCA 1 cut(s) 385
Mva1269I GAATGC 2 cut(s) 38, 101
NdeI CATATG 1 cut(s) 851
NdeII GATC 5 cut(s) 439, 482, 526, 832, 872
NlaIII CATG 8 cut(s) 72, 131, 248, 278, 451, 494, 542, 749
NsiI ATGCAT 1 cut(s) 38
NspI RCATGY 2 cut(s) 248, 451
NspV TTCGAA 1 cut(s) 675
PagI TCATGA 1 cut(s) 274
PcsI WCGNNNNNNNCGW 1 cut(s) 883
PctI GAATGC 2 cut(s) 38, 101
PdmI GAANNNNTTC 2 cut(s) 91, 671
PfeI GAWTC 3 cut(s) 572, 716, 794
PflFI GACNNNGTC 1 cut(s) 882
PkrI GCNGC 1 cut(s) 514
PmaCI CACGTG 1 cut(s) 500
PmlI CACGTG 1 cut(s) 500
Ppu21I YACGTR 1 cut(s) 500
PspCI CACGTG 1 cut(s) 500
PsuI RGATCY 2 cut(s) 482, 872
PsyI GACNNNGTC 1 cut(s) 882
RsaI GTAC 2 cut(s) 543, 735
RsaNI GTAC 2 cut(s) 542, 734
RseI CAYNNNNRTG 1 cut(s) 112
SaqAI TTAA 5 cut(s) 233, 285, 392, 659, 690
SatI GCNGC 1 cut(s) 513
Sau3AI GATC 5 cut(s) 439, 482, 526, 832, 872
SfaNI GCATC 2 cut(s) 484, 739
SfcI CTRYAG 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 675
SmiMI CAYNNNNRTG 1 cut(s) 112
Sse9I AATT 6 cut(s) 48, 258, 408, 652, 786, 837
SsiI CCGC 1 cut(s) 820
SspI AATATT 2 cut(s) 123, 688
SspMI CTAG 2 cut(s) 8, 428
TaaI ACNGT 3 cut(s) 56, 111, 664
TaiI ACGT 4 cut(s) 223, 502, 595, 883
TaqI TCGA 6 cut(s) 152, 168, 333, 675, 877, 886
TasI AATT 6 cut(s) 48, 258, 408, 652, 786, 837
TatI WGTACW 1 cut(s) 733
TfiI GAWTC 3 cut(s) 572, 716, 794
Tru1I TTAA 5 cut(s) 233, 285, 392, 659, 690
Tru9I TTAA 5 cut(s) 233, 285, 392, 659, 690
TseI GCWGC 1 cut(s) 512
TspDTI ATGAA 4 cut(s) 57, 75, 263, 527
Tth111I GACNNNGTC 1 cut(s) 882
XapI RAATTY 1 cut(s) 48
XceI RCATGY 2 cut(s) 248, 451
XmnI GAANNNNTTC 2 cut(s) 91, 671
XspI CTAG 2 cut(s) 8, 428
Zsp2I ATGCAT 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.